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UniProtKB - P0AEA5 (CYOE_ECOLI)
Protein
Protoheme IX farnesyltransferase
Gene
cyoE
Organism
Escherichia coli (strain K12)
Status
Functioni
Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.
2 PublicationsMiscellaneous
Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.
Catalytic activityi
- EC:2.5.1.1411 Publication
: heme O biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes heme O from protoheme. This subpathway is part of the pathway heme O biosynthesis, which is itself part of Porphyrin-containing compound metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes heme O from protoheme, the pathway heme O biosynthesis and in Porphyrin-containing compound metabolism.
GO - Molecular functioni
- farnesyltranstransferase activity Source: GO_Central
- protoheme IX farnesyltransferase activity Source: EcoliWiki
GO - Biological processi
- cellular respiration Source: GO_Central
- heme biosynthetic process Source: GO_Central
- heme O biosynthetic process Source: EcoliWiki
Keywordsi
Molecular function | Transferase |
Biological process | Heme biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:HEMEOSYN-MONOMER |
BRENDAi | 2.5.1.141, 2026 |
UniPathwayi | UPA00834;UER00712 |
Names & Taxonomyi
Protein namesi | Recommended name: Protoheme IX farnesyltransferase (EC:2.5.1.1411 Publication)Alternative name(s): Heme B farnesyltransferase Heme O synthase1 Publication |
Gene namesi | Name:cyoE Ordered Locus Names:b0428, JW0418 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | CytoplasmicCurated | 9 | |
Transmembranei | 10 – 28 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 29 – 37 | PeriplasmicCurated | 9 | |
Transmembranei | 38 – 56 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 57 – 78 | CytoplasmicCuratedAdd BLAST | 22 | |
Transmembranei | 79 – 97 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 98 – 107 | PeriplasmicCurated | 10 | |
Transmembranei | 108 – 126 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 127 – 197 | CytoplasmicCuratedAdd BLAST | 71 | |
Transmembranei | 198 – 216 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 217 – 228 | PeriplasmicCuratedAdd BLAST | 12 | |
Transmembranei | 229 – 247 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 248 – 268 | CytoplasmicCuratedAdd BLAST | 21 | |
Transmembranei | 269 – 287 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 288 – 296 | PeriplasmicCurated | 9 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000162895 | 1 – 296 | Protoheme IX farnesyltransferaseAdd BLAST | 296 |
Proteomic databases
PaxDbi | P0AEA5 |
PRIDEi | P0AEA5 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259839, 24 interactors |
DIPi | DIP-47946N |
STRINGi | 511145.b0428 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0109, Bacteria |
HOGENOMi | CLU_029631_0_0_6 |
InParanoidi | P0AEA5 |
PhylomeDBi | P0AEA5 |
Family and domain databases
CDDi | cd13957, PT_UbiA_Cox10, 1 hit |
Gene3Di | 1.10.357.140, 1 hit |
HAMAPi | MF_00154, CyoE_CtaB, 1 hit |
InterProi | View protein in InterPro IPR006369, Protohaem_IX_farnesylTrfase IPR000537, UbiA_prenyltransferase IPR030470, UbiA_prenylTrfase_CS IPR044878, UbiA_sf |
PANTHERi | PTHR43448, PTHR43448, 1 hit |
Pfami | View protein in Pfam PF01040, UbiA, 1 hit |
TIGRFAMsi | TIGR01473, cyoE_ctaB, 1 hit |
PROSITEi | View protein in PROSITE PS00943, UBIA, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AEA5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMFKQYLQVT KPGIIFGNLI SVIGGFLLAS KGSIDYPLFI YTLVGVSLVV
60 70 80 90 100
ASGCVFNNYI DRDIDRKMER TKNRVLVKGL ISPAVSLVYA TLLGIAGFML
110 120 130 140 150
LWFGANPLAC WLGVMGFVVY VGVYSLYMKR HSVYGTLIGS LSGAAPPVIG
160 170 180 190 200
YCAVTGEFDS GAAILLAIFS LWQMPHSYAI AIFRFKDYQA ANIPVLPVVK
210 220 230 240 250
GISVAKNHIT LYIIAFAVAT LMLSLGGYAG YKYLVVAAAV SVWWLGMALR
260 270 280 290
GYKVADDRIW ARKLFGFSII AITALSVMMS VDFMVPDSHT LLAAVW
Sequence cautioni
The sequence AAB40184 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05492 Genomic DNA Translation: AAA23635.1 U82664 Genomic DNA Translation: AAB40184.1 Different initiation. U00096 Genomic DNA Translation: AAC73531.1 AP009048 Genomic DNA Translation: BAE76208.1 |
PIRi | E42226 |
RefSeqi | NP_414962.1, NC_000913.3 WP_000971336.1, NZ_SSZK01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC73531; AAC73531; b0428 BAE76208; BAE76208; BAE76208 |
GeneIDi | 66671270 945073 |
KEGGi | ecj:JW0418 eco:b0428 |
PATRICi | fig|1411691.4.peg.1849 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05492 Genomic DNA Translation: AAA23635.1 U82664 Genomic DNA Translation: AAB40184.1 Different initiation. U00096 Genomic DNA Translation: AAC73531.1 AP009048 Genomic DNA Translation: BAE76208.1 |
PIRi | E42226 |
RefSeqi | NP_414962.1, NC_000913.3 WP_000971336.1, NZ_SSZK01000009.1 |
3D structure databases
SMRi | P0AEA5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259839, 24 interactors |
DIPi | DIP-47946N |
STRINGi | 511145.b0428 |
Proteomic databases
PaxDbi | P0AEA5 |
PRIDEi | P0AEA5 |
Genome annotation databases
EnsemblBacteriai | AAC73531; AAC73531; b0428 BAE76208; BAE76208; BAE76208 |
GeneIDi | 66671270 945073 |
KEGGi | ecj:JW0418 eco:b0428 |
PATRICi | fig|1411691.4.peg.1849 |
Organism-specific databases
EchoBASEi | EB0179 |
Phylogenomic databases
eggNOGi | COG0109, Bacteria |
HOGENOMi | CLU_029631_0_0_6 |
InParanoidi | P0AEA5 |
PhylomeDBi | P0AEA5 |
Enzyme and pathway databases
UniPathwayi | UPA00834;UER00712 |
BioCyci | EcoCyc:HEMEOSYN-MONOMER |
BRENDAi | 2.5.1.141, 2026 |
Miscellaneous databases
PROi | PR:P0AEA5 |
Family and domain databases
CDDi | cd13957, PT_UbiA_Cox10, 1 hit |
Gene3Di | 1.10.357.140, 1 hit |
HAMAPi | MF_00154, CyoE_CtaB, 1 hit |
InterProi | View protein in InterPro IPR006369, Protohaem_IX_farnesylTrfase IPR000537, UbiA_prenyltransferase IPR030470, UbiA_prenylTrfase_CS IPR044878, UbiA_sf |
PANTHERi | PTHR43448, PTHR43448, 1 hit |
Pfami | View protein in Pfam PF01040, UbiA, 1 hit |
TIGRFAMsi | TIGR01473, cyoE_ctaB, 1 hit |
PROSITEi | View protein in PROSITE PS00943, UBIA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CYOE_ECOLI | |
Accessioni | P0AEA5Primary (citable) accession number: P0AEA5 Secondary accession number(s): P18404, P77115, Q2MBZ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | February 23, 2022 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families