UniProtKB - P0AE88 (CPXR_ECOLI)
Protein
Transcriptional regulatory protein CpxR
Gene
cpxR
Organism
Escherichia coli (strain K12)
Status
Functioni
Response regulator member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA (PubMed:7883164, PubMed:9401031). Required for efficient binding of stationary phase cells to hydrophobic surfaces, part of the process of biofilm formation (PubMed:11830644). Induced upon cell surface binding, subsequently induces genes it controls (cpxP, dsbA and spy, degP is only partially induced) (PubMed:11830644). Binds and activates transcription from the degP promoter (PubMed:7883164); binding is enhanced by phosphorylation (PubMed:9401031). This system combats a variety of extracytoplasmic protein-mediated toxicities by increasing the transcription of the periplasmic protease, DegP in concert with sigma factor E (PubMed:7883164), as well as that of CpxP protein. Other downstream effectors may include SrkA (PubMed:23416055).4 Publications
Activity regulationi
The two-component system is activated by envelope stress such as overexpression of some (misfolded) periplasmic proteins (PubMed:7883164, PubMed:9351822). Activated by spheroplasting (which removes the periplasm) in an autoregulatory cpxA-cpxR-dependent fashion (PubMed:10972835). Cpx two-component system is activated at pH 8.0; in a degP deletion mutant activation is halved (PubMed:9473036, PubMed:16166523). The CpxA kinase activity is inhibited by periplasmic accessory protein CpxP; proteolysis of CpxP relieves inhibition (PubMed:16166523, PubMed:17259177, PubMed:25207645).5 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 131 – 230 | OmpR/PhoB-typePROSITE-ProRule annotationAdd BLAST | 100 |
GO - Molecular functioni
- DNA-binding transcription activator activity Source: GO_Central
- DNA-binding transcription factor activity Source: EcoCyc
- phosphorelay response regulator activity Source: CACAO
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- cell adhesion Source: UniProtKB-KW
- negative regulation of transcription, DNA-templated Source: EcoCyc
- positive regulation of transcription, DNA-templated Source: EcoCyc
- regulation of cell-substrate adhesion Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Cell adhesion, Stress response, Transcription, Transcription regulation, Two-component regulatory system |
Enzyme and pathway databases
BioCyci | EcoCyc:CPXR-MONOMER MetaCyc:CPXR-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional regulatory protein CpxR |
Gene namesi | Name:cpxR Synonyms:yiiA Ordered Locus Names:b3912, JW3883 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
- cytosol Source: EcoCyc
- protein-DNA complex Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Loss of the Cpx envelope stress response (PubMed:10972835). A double cpxR-cpxA mutant decreases transcription of degP (PubMed:7883164). Cells are less sensitive to killing by nalidixic acid; double cpxR-srkA disruption mutants are as sensitive to killing as single srkA mutants, suggesting the SrkA protein kinase is partially regulated by CpxR. Hypersensitive to alkaline pH (greater than pH 8.8) (PubMed:9473036). Decreased numbers of stationary phase cells bind to hydrophobic surfaces, cellular adhesion has altered dynamic properties; no induction of cpxR when cells bind to hydrophobic surfaces (PubMed:11830644).5 Publications
Miscellaneous databases
PHI-basei | PHI:4523 PHI:8273 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081078 | 1 – 232 | Transcriptional regulatory protein CpxRAdd BLAST | 232 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 51 | 4-aspartylphosphatePROSITE-ProRule annotation | 1 |
Post-translational modificationi
Phosphorylated by CpxA.1 Publication2 Publications
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P0AE88 |
PaxDbi | P0AE88 |
PRIDEi | P0AE88 |
Expressioni
Inductioni
Induced about 3-fold when stationary phase cells bind to hydrophobic surfaces; requires direct contact with hydrophobic surfaces for up-regulation of Cpx activity (PubMed:11830644).1 Publication
Interactioni
Subunit structurei
Interacts with cognate sensor kinase CpxA.
1 PublicationBinary interactionsi
Hide detailsP0AE88
With | #Exp. | IntAct |
---|---|---|
cpxA [P0AE82] | 3 | EBI-550918,EBI-9141330 |
lysA [P00861] | 4 | EBI-550918,EBI-553837 |
secB [P0AG86] | 3 | EBI-550918,EBI-555877 |
Protein-protein interaction databases
BioGRIDi | 4262645, 290 interactors 852701, 2 interactors |
DIPi | DIP-47991N |
IntActi | P0AE88, 11 interactors |
STRINGi | 511145.b3912 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AE88 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 115 | Response regulatoryPROSITE-ProRule annotationAdd BLAST | 113 |
Phylogenomic databases
eggNOGi | COG0745, Bacteria |
HOGENOMi | CLU_000445_30_4_6 |
InParanoidi | P0AE88 |
PhylomeDBi | P0AE88 |
Family and domain databases
CDDi | cd00156, REC, 1 hit cd00383, trans_reg_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR001867, OmpR/PhoB-type_DNA-bd IPR001789, Sig_transdc_resp-reg_receiver IPR039420, WalR-like IPR036388, WH-like_DNA-bd_sf |
PANTHERi | PTHR26402, PTHR26402, 1 hit |
Pfami | View protein in Pfam PF00072, Response_reg, 1 hit PF00486, Trans_reg_C, 1 hit |
SMARTi | View protein in SMART SM00448, REC, 1 hit SM00862, Trans_reg_C, 1 hit |
SUPFAMi | SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS51755, OMPR_PHOB, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AE88-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNKILLVDDD RELTSLLKEL LEMEGFNVIV AHDGEQALDL LDDSIDLLLL
60 70 80 90 100
DVMMPKKNGI DTLKALRQTH QTPVIMLTAR GSELDRVLGL ELGADDYLPK
110 120 130 140 150
PFNDRELVAR IRAILRRSHW SEQQQNNDNG SPTLEVDALV LNPGRQEASF
160 170 180 190 200
DGQTLELTGT EFTLLYLLAQ HLGQVVSREH LSQEVLGKRL TPFDRAIDMH
210 220 230
ISNLRRKLPD RKDGHPWFKT LRGRGYLMVS AS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L14579 Unassigned DNA Translation: AAC36868.1 L19201 Genomic DNA Translation: AAB03045.1 U00096 Genomic DNA Translation: AAC76894.1 AP009048 Genomic DNA Translation: BAE77397.1 X13307 Genomic DNA Translation: CAA31686.1 M36795 Genomic DNA Translation: AAA23599.1 |
PIRi | C65197 |
RefSeqi | NP_418348.1, NC_000913.3 WP_001033722.1, NZ_STEB01000017.1 |
Genome annotation databases
EnsemblBacteriai | AAC76894; AAC76894; b3912 BAE77397; BAE77397; BAE77397 |
GeneIDi | 52078816 948404 |
KEGGi | ecj:JW3883 eco:b3912 |
PATRICi | fig|1411691.4.peg.2792 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L14579 Unassigned DNA Translation: AAC36868.1 L19201 Genomic DNA Translation: AAB03045.1 U00096 Genomic DNA Translation: AAC76894.1 AP009048 Genomic DNA Translation: BAE77397.1 X13307 Genomic DNA Translation: CAA31686.1 M36795 Genomic DNA Translation: AAA23599.1 |
PIRi | C65197 |
RefSeqi | NP_418348.1, NC_000913.3 WP_001033722.1, NZ_STEB01000017.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4UHJ | X-ray | 1.90 | A/B/C | 1-123 | [»] | |
4UHK | X-ray | 2.60 | A/B/C | 1-123 | [»] | |
4UHS | X-ray | 5.00 | A/B/C | 1-123 | [»] | |
4UHT | X-ray | 1.15 | A/B | 131-231 | [»] | |
SMRi | P0AE88 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262645, 290 interactors 852701, 2 interactors |
DIPi | DIP-47991N |
IntActi | P0AE88, 11 interactors |
STRINGi | 511145.b3912 |
Proteomic databases
jPOSTi | P0AE88 |
PaxDbi | P0AE88 |
PRIDEi | P0AE88 |
Genome annotation databases
EnsemblBacteriai | AAC76894; AAC76894; b3912 BAE77397; BAE77397; BAE77397 |
GeneIDi | 52078816 948404 |
KEGGi | ecj:JW3883 eco:b3912 |
PATRICi | fig|1411691.4.peg.2792 |
Organism-specific databases
EchoBASEi | EB0019 |
Phylogenomic databases
eggNOGi | COG0745, Bacteria |
HOGENOMi | CLU_000445_30_4_6 |
InParanoidi | P0AE88 |
PhylomeDBi | P0AE88 |
Enzyme and pathway databases
BioCyci | EcoCyc:CPXR-MONOMER MetaCyc:CPXR-MONOMER |
Miscellaneous databases
PHI-basei | PHI:4523 PHI:8273 |
PROi | PR:P0AE88 |
Family and domain databases
CDDi | cd00156, REC, 1 hit cd00383, trans_reg_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR001867, OmpR/PhoB-type_DNA-bd IPR001789, Sig_transdc_resp-reg_receiver IPR039420, WalR-like IPR036388, WH-like_DNA-bd_sf |
PANTHERi | PTHR26402, PTHR26402, 1 hit |
Pfami | View protein in Pfam PF00072, Response_reg, 1 hit PF00486, Trans_reg_C, 1 hit |
SMARTi | View protein in SMART SM00448, REC, 1 hit SM00862, Trans_reg_C, 1 hit |
SUPFAMi | SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS51755, OMPR_PHOB, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CPXR_ECOLI | |
Accessioni | P0AE88Primary (citable) accession number: P0AE88 Secondary accession number(s): P16244, P76777, Q2M8K9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | December 2, 2020 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references