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Protein

Transcriptional regulatory protein CpxR

Gene

cpxR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Response regulator member of the two-component regulatory system CpxA/CpxR which responds to envelope stress response by activating expression of downstream genes including cpxP, degP, dsbA and ppiA (PubMed:7883164, PubMed:9401031). Required for efficient binding of stationary phase cells to hydrophobic surfaces, part of the process of biofilm formation (PubMed:11830644). Induced upon cell surface binding, subsequently induces genes it controls (cpxP, dsbA and spy, degP is only partially induced) (PubMed:11830644). Binds and activates transcription from the degP promoter (PubMed:7883164); binding is enhanced by phosphorylation (PubMed:9401031). This system combats a variety of extracytoplasmic protein-mediated toxicities by increasing the transcription of the periplasmic protease, DegP in concert with sigma factor E (PubMed:7883164), as well as that of CpxP protein. Other downstream effectors may include SrkA (PubMed:23416055).4 Publications

Activity regulationi

The two-component system is activated by envelope stress such as overexpression of some (misfolded) periplasmic proteins (PubMed:7883164, PubMed:9351822). Activated by spheroplasting (which removes the periplasm) in an autoregulatory cpxA-cpxR-dependent fashion (PubMed:10972835). Cpx two-component system is activated at pH 8.0; in a degP deletion mutant activation is halved (PubMed:9473036, PubMed:16166523). The CpxA kinase activity is inhibited by periplasmic accessory protein CpxP; proteolysis of CpxP relieves inhibition (PubMed:16166523, PubMed:17259177, PubMed:25207645).5 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi131 – 230OmpR/PhoB-typePROSITE-ProRule annotationAdd BLAST100

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA-binding transcription factor activity Source: EcoCyc
  • phosphorelay response regulator activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processCell adhesion, Stress response, Transcription, Transcription regulation, Two-component regulatory system

Enzyme and pathway databases

BioCyciEcoCyc:CPXR-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein CpxR
Gene namesi
Name:cpxR
Synonyms:yiiA
Ordered Locus Names:b3912, JW3883
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10020 cpxR

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Loss of the Cpx envelope stress response (PubMed:10972835). A double cpxR-cpxA mutant decreases transcription of degP (PubMed:7883164). Cells are less sensitive to killing by nalidixic acid; double cpxR-srkA disruption mutants are as sensitive to killing as single srkA mutants, suggesting the SrkA protein kinase is partially regulated by CpxR. Hypersensitive to alkaline pH (greater than pH 8.8) (PubMed:9473036). Decreased numbers of stationary phase cells bind to hydrophobic surfaces, cellular adhesion has altered dynamic properties; no induction of cpxR when cells bind to hydrophobic surfaces (PubMed:11830644).5 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000810781 – 232Transcriptional regulatory protein CpxRAdd BLAST232

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei514-aspartylphosphatePROSITE-ProRule annotation1

Post-translational modificationi

Phosphorylated by CpxA.1 Publication2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP0AE88
PaxDbiP0AE88
PRIDEiP0AE88

Expressioni

Inductioni

Induced about 3-fold when stationary phase cells bind to hydrophobic surfaces; requires direct contact with hydrophobic surfaces for up-regulation of Cpx activity (PubMed:11830644).1 Publication

Interactioni

Subunit structurei

Interacts with cognate sensor kinase CpxA.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi4262645, 290 interactors
DIPiDIP-47991N
IntActiP0AE88, 11 interactors
STRINGi316385.ECDH10B_4102

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0AE88
SMRiP0AE88
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 115Response regulatoryPROSITE-ProRule annotationAdd BLAST113

Phylogenomic databases

eggNOGiENOG4105E3W Bacteria
COG0745 LUCA
HOGENOMiHOG000034819
InParanoidiP0AE88
KOiK07662
PhylomeDBiP0AE88

Family and domain databases

CDDicd00156 REC, 1 hit
cd00383 trans_reg_C, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR001867 OmpR/PhoB-type_DNA-bd
IPR001789 Sig_transdc_resp-reg_receiver
IPR039420 WalR-like
IPR036388 WH-like_DNA-bd_sf
PANTHERiPTHR26402 PTHR26402, 1 hit
PfamiView protein in Pfam
PF00072 Response_reg, 1 hit
PF00486 Trans_reg_C, 1 hit
SMARTiView protein in SMART
SM00448 REC, 1 hit
SM00862 Trans_reg_C, 1 hit
SUPFAMiSSF52172 SSF52172, 1 hit
PROSITEiView protein in PROSITE
PS51755 OMPR_PHOB, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit

Sequencei

Sequence statusi: Complete.

P0AE88-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNKILLVDDD RELTSLLKEL LEMEGFNVIV AHDGEQALDL LDDSIDLLLL
60 70 80 90 100
DVMMPKKNGI DTLKALRQTH QTPVIMLTAR GSELDRVLGL ELGADDYLPK
110 120 130 140 150
PFNDRELVAR IRAILRRSHW SEQQQNNDNG SPTLEVDALV LNPGRQEASF
160 170 180 190 200
DGQTLELTGT EFTLLYLLAQ HLGQVVSREH LSQEVLGKRL TPFDRAIDMH
210 220 230
ISNLRRKLPD RKDGHPWFKT LRGRGYLMVS AS
Length:232
Mass (Da):26,312
Last modified:December 6, 2005 - v1
Checksum:i667FC2B246082F46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14579 Unassigned DNA Translation: AAC36868.1
L19201 Genomic DNA Translation: AAB03045.1
U00096 Genomic DNA Translation: AAC76894.1
AP009048 Genomic DNA Translation: BAE77397.1
X13307 Genomic DNA Translation: CAA31686.1
M36795 Genomic DNA Translation: AAA23599.1
PIRiC65197
RefSeqiNP_418348.1, NC_000913.3
WP_001033722.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76894; AAC76894; b3912
BAE77397; BAE77397; BAE77397
GeneIDi948404
KEGGiecj:JW3883
eco:b3912
PATRICifig|1411691.4.peg.2792

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14579 Unassigned DNA Translation: AAC36868.1
L19201 Genomic DNA Translation: AAB03045.1
U00096 Genomic DNA Translation: AAC76894.1
AP009048 Genomic DNA Translation: BAE77397.1
X13307 Genomic DNA Translation: CAA31686.1
M36795 Genomic DNA Translation: AAA23599.1
PIRiC65197
RefSeqiNP_418348.1, NC_000913.3
WP_001033722.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4UHJX-ray1.90A/B/C1-123[»]
4UHKX-ray2.60A/B/C1-123[»]
4UHSX-ray5.00A/B/C1-123[»]
4UHTX-ray1.15A/B131-231[»]
ProteinModelPortaliP0AE88
SMRiP0AE88
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262645, 290 interactors
DIPiDIP-47991N
IntActiP0AE88, 11 interactors
STRINGi316385.ECDH10B_4102

Proteomic databases

EPDiP0AE88
PaxDbiP0AE88
PRIDEiP0AE88

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76894; AAC76894; b3912
BAE77397; BAE77397; BAE77397
GeneIDi948404
KEGGiecj:JW3883
eco:b3912
PATRICifig|1411691.4.peg.2792

Organism-specific databases

EchoBASEiEB0019
EcoGeneiEG10020 cpxR

Phylogenomic databases

eggNOGiENOG4105E3W Bacteria
COG0745 LUCA
HOGENOMiHOG000034819
InParanoidiP0AE88
KOiK07662
PhylomeDBiP0AE88

Enzyme and pathway databases

BioCyciEcoCyc:CPXR-MONOMER

Miscellaneous databases

PROiPR:P0AE88

Family and domain databases

CDDicd00156 REC, 1 hit
cd00383 trans_reg_C, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR001867 OmpR/PhoB-type_DNA-bd
IPR001789 Sig_transdc_resp-reg_receiver
IPR039420 WalR-like
IPR036388 WH-like_DNA-bd_sf
PANTHERiPTHR26402 PTHR26402, 1 hit
PfamiView protein in Pfam
PF00072 Response_reg, 1 hit
PF00486 Trans_reg_C, 1 hit
SMARTiView protein in SMART
SM00448 REC, 1 hit
SM00862 Trans_reg_C, 1 hit
SUPFAMiSSF52172 SSF52172, 1 hit
PROSITEiView protein in PROSITE
PS51755 OMPR_PHOB, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCPXR_ECOLI
AccessioniPrimary (citable) accession number: P0AE88
Secondary accession number(s): P16244, P76777, Q2M8K9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 7, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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