UniProtKB - P0AE16 (AMPG_ECOLI)
Protein
Anhydromuropeptide permease
Gene
ampG
Organism
Escherichia coli (strain K12)
Status
Functioni
Permease involved in cell wall peptidoglycan recycling (PubMed:8878601, PubMed:12426329). Transports, from the periplasm into the cytoplasm, the disaccharide N-acetylglucosaminyl-beta-1,4-anhydro-N-acetylmuramic acid (GlcNAc-anhMurNAc) and GlcNAc-anhMurNAc-peptides (PubMed:12426329). Transport is dependent on the proton motive force (PubMed:12426329). AmpG is also involved in beta-lactamase induction (PubMed:7773404).3 Publications
Activity regulationi
Uptake is inhibited by carbonyl cyanide m-chlorophenylhydrazone (CCCP).1 Publication
GO - Molecular functioni
- solute:proton symporter activity Source: GO_Central
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan transport Source: GO_Central
Keywordsi
Biological process | Cell wall biogenesis/degradation, Symport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:AMPG-MONOMER MetaCyc:AMPG-MONOMER |
Protein family/group databases
TCDBi | 2.A.1.25.2, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Anhydromuropeptide permease1 PublicationAlternative name(s): AmpG permease2 Publications Muropeptide:H(+) symporterCurated |
Gene namesi | Name:ampG1 Publication Ordered Locus Names:b0433, JW0423 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 2 Publications; Multi-pass membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | Cytoplasmic1 PublicationAdd BLAST | 11 | |
Transmembranei | 12 – 32 | Helical1 PublicationAdd BLAST | 21 | |
Topological domaini | 33 – 47 | Periplasmic1 PublicationAdd BLAST | 15 | |
Transmembranei | 48 – 61 | Helical1 PublicationAdd BLAST | 14 | |
Topological domaini | 62 – 81 | Cytoplasmic1 PublicationAdd BLAST | 20 | |
Transmembranei | 82 – 105 | Helical1 PublicationAdd BLAST | 24 | |
Topological domaini | 106 | Periplasmic1 Publication | 1 | |
Transmembranei | 107 – 124 | Helical1 PublicationAdd BLAST | 18 | |
Topological domaini | 125 – 221 | Cytoplasmic1 PublicationAdd BLAST | 97 | |
Transmembranei | 222 – 240 | Helical1 PublicationAdd BLAST | 19 | |
Topological domaini | 241 – 264 | Periplasmic1 PublicationAdd BLAST | 24 | |
Transmembranei | 265 – 284 | Helical1 PublicationAdd BLAST | 20 | |
Topological domaini | 285 – 287 | Cytoplasmic1 Publication | 3 | |
Transmembranei | 288 – 303 | Helical1 PublicationAdd BLAST | 16 | |
Topological domaini | 304 – 327 | Periplasmic1 PublicationAdd BLAST | 24 | |
Transmembranei | 328 – 346 | Helical1 PublicationAdd BLAST | 19 | |
Topological domaini | 347 – 421 | Cytoplasmic1 PublicationAdd BLAST | 75 | |
Transmembranei | 422 – 453 | Helical1 PublicationAdd BLAST | 32 | |
Topological domaini | 454 – 457 | Periplasmic1 Publication | 4 | |
Transmembranei | 458 – 485 | Helical1 PublicationAdd BLAST | 28 | |
Topological domaini | 486 – 491 | Cytoplasmic2 Publications | 6 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084830 | 1 – 491 | Anhydromuropeptide permeaseAdd BLAST | 491 |
Proteomic databases
PaxDbi | P0AE16 |
PRIDEi | P0AE16 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259838, 195 interactors |
STRINGi | 511145.b0433 |
Family & Domainsi
Domaini
Four hydrophobic segments are not embedded in the cytoplasmic membrane and form two large cytoplasmic loops.1 Publication
Sequence similaritiesi
Belongs to the major facilitator superfamily.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2223, Bacteria |
HOGENOMi | CLU_029352_1_0_6 |
InParanoidi | P0AE16 |
PhylomeDBi | P0AE16 |
Family and domain databases
InterProi | View protein in InterPro IPR004752, AmpG_permease/AT-1 IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
PANTHERi | PTHR12778, PTHR12778, 1 hit |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AE16-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSQYLRIFQ QPRSAILLIL GFASGLPLAL TSGTLQAWMT VENIDLKTIG
60 70 80 90 100
FFSLVGQAYV FKFLWSPLMD RYTPPFFGRR RGWLLATQIL LLVAIAAMGF
110 120 130 140 150
LEPGTQLRWM AALAVVIAFC SASQDIVFDA WKTDVLPAEE RGAGAAISVL
160 170 180 190 200
GYRLGMLVSG GLALWLADKW LGWQGMYWLM AALLIPCIIA TLLAPEPTDT
210 220 230 240 250
IPVPKTLEQA VVAPLRDFFG RNNAWLILLL IVLYKLGDAF AMSLTTTFLI
260 270 280 290 300
RGVGFDAGEV GVVNKTLGLL ATIVGALYGG ILMQRLSLFR ALLIFGILQG
310 320 330 340 350
ASNAGYWLLS ITDKHLYSMG AAVFFENLCG GMGTSAFVAL LMTLCNKSFS
360 370 380 390 400
ATQFALLSAL SAVGRVYVGP VAGWFVEAHG WSTFYLFSVA AAVPGLILLL
410 420 430 440 450
VCRQTLEYTR VNDNFISRTA YPAGYAFAMW TLAAGVSLLA VWLLLLTMDA
460 470 480 490
LDLTHFSFLP ALLEVGVLVA LSGVVLGGLL DYLALRKTHL T
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 151 | G → D in non-inducible mutant SN0301-1. | 1 | |
Natural varianti | 268 | G → D in non-inducible mutant SN0301-3. | 1 | |
Natural varianti | 373 | G → D in non-inducible mutant SN0301-5. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S67816 Genomic DNA Translation: AAB28884.1 X82158 mRNA Translation: CAA57651.1 X82159 mRNA Translation: CAA57652.1 X82160 mRNA Translation: CAA57653.1 U82664 Genomic DNA Translation: AAB40189.1 U00096 Genomic DNA Translation: AAC73536.1 AP009048 Genomic DNA Translation: BAE76213.1 |
PIRi | S37391 |
RefSeqi | NP_414967.1, NC_000913.3 WP_000098429.1, NZ_SSZK01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC73536; AAC73536; b0433 BAE76213; BAE76213; BAE76213 |
GeneIDi | 57731612 946438 |
KEGGi | ecj:JW0423 eco:b0433 |
PATRICi | fig|1411691.4.peg.1844 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S67816 Genomic DNA Translation: AAB28884.1 X82158 mRNA Translation: CAA57651.1 X82159 mRNA Translation: CAA57652.1 X82160 mRNA Translation: CAA57653.1 U82664 Genomic DNA Translation: AAB40189.1 U00096 Genomic DNA Translation: AAC73536.1 AP009048 Genomic DNA Translation: BAE76213.1 |
PIRi | S37391 |
RefSeqi | NP_414967.1, NC_000913.3 WP_000098429.1, NZ_SSZK01000009.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4259838, 195 interactors |
STRINGi | 511145.b0433 |
Protein family/group databases
TCDBi | 2.A.1.25.2, the major facilitator superfamily (mfs) |
Proteomic databases
PaxDbi | P0AE16 |
PRIDEi | P0AE16 |
Genome annotation databases
EnsemblBacteriai | AAC73536; AAC73536; b0433 BAE76213; BAE76213; BAE76213 |
GeneIDi | 57731612 946438 |
KEGGi | ecj:JW0423 eco:b0433 |
PATRICi | fig|1411691.4.peg.1844 |
Organism-specific databases
EchoBASEi | EB2100 |
Phylogenomic databases
eggNOGi | COG2223, Bacteria |
HOGENOMi | CLU_029352_1_0_6 |
InParanoidi | P0AE16 |
PhylomeDBi | P0AE16 |
Enzyme and pathway databases
BioCyci | EcoCyc:AMPG-MONOMER MetaCyc:AMPG-MONOMER |
Miscellaneous databases
PROi | PR:P0AE16 |
Family and domain databases
InterProi | View protein in InterPro IPR004752, AmpG_permease/AT-1 IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
PANTHERi | PTHR12778, PTHR12778, 1 hit |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AMPG_ECOLI | |
Accessioni | P0AE16Primary (citable) accession number: P0AE16 Secondary accession number(s): P36670, Q2MBZ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | February 10, 2021 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families