UniProtKB - P0AE10 (AHPC_ECO57)
Protein
Alkyl hydroperoxide reductase C
Gene
ahpC
Organism
Escherichia coli O157:H7
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.By similarity
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by AhpF.By similarity
Catalytic activityi
- EC:1.11.1.26By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 47 | Cysteine sulfenic acid (-SOH) intermediateBy similarity | 1 |
GO - Molecular functioni
- alkylhydroperoxide reductase activity Source: UniProtKB-EC
- peroxidase activity Source: UniProtKB-KW
- peroxiredoxin activity Source: InterPro
GO - Biological processi
- response to oxidative stress Source: InterPro
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Names & Taxonomyi
Protein namesi | Recommended name: Alkyl hydroperoxide reductase C (EC:1.11.1.26By similarity)Alternative name(s): Alkyl hydroperoxide reductase protein C22 Peroxiredoxin SCRP-23 Sulfate starvation-induced protein 8 Short name: SSI8 Thioredoxin peroxidase |
Gene namesi | Name:ahpC Ordered Locus Names:Z0749, ECs0644 |
Organismi | Escherichia coli O157:H7 |
Taxonomic identifieri | 83334 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000135116 | 2 – 187 | Alkyl hydroperoxide reductase CAdd BLAST | 186 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 17 | N6-acetyllysineBy similarity | 1 | |
Disulfide bondi | 47 | Interchain (with C-166); in linked formBy similarity | ||
Modified residuei | 93 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 153 | N6-acetyllysineBy similarity | 1 | |
Disulfide bondi | 166 | Interchain (with C-47); in linked formBy similarity | ||
Modified residuei | 169 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 171 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, Disulfide bondInteractioni
Subunit structurei
Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer.
By similarityProtein-protein interaction databases
STRINGi | 155864.EDL933_0677 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 157 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 156 |
Sequence similaritiesi
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | COG0450, Bacteria |
HOGENOMi | CLU_042529_21_3_6 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR017559, AhpC IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
PANTHERi | PTHR10681:SF121, PTHR10681:SF121, 1 hit |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR03137, AhpC, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AE10-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLINTKIKP FKNQAFKNGE FIEITEKDTE GRWSVFFFYP ADFTFVCPTE
60 70 80 90 100
LGDVADHYEE LQKLGVDVYA VSTDTHFTHK AWHSSSETIA KIKYAMIGDP
110 120 130 140 150
TGALTRNFDN MREDEGLADR ATFVVDPQGI IQAIEVTAEG IGRDASDLLR
160 170 180
KIKAAQYVAS HPGEVCPAKW KEGEATLAPS LDLVGKI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005174 Genomic DNA Translation: AAG54940.1 BA000007 Genomic DNA Translation: BAB34067.1 |
PIRi | D90709 H85559 |
RefSeqi | NP_308671.1, NC_002695.1 WP_000052796.1, NZ_SWKA01000005.1 |
Genome annotation databases
EnsemblBacteriai | AAG54940; AAG54940; Z0749 BAB34067; BAB34067; ECs_0644 |
GeneIDi | 58462627 917003 |
KEGGi | ece:Z0749 ecs:ECs0644 |
PATRICi | fig|386585.9.peg.755 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005174 Genomic DNA Translation: AAG54940.1 BA000007 Genomic DNA Translation: BAB34067.1 |
PIRi | D90709 H85559 |
RefSeqi | NP_308671.1, NC_002695.1 WP_000052796.1, NZ_SWKA01000005.1 |
3D structure databases
SMRi | P0AE10 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 155864.EDL933_0677 |
Genome annotation databases
EnsemblBacteriai | AAG54940; AAG54940; Z0749 BAB34067; BAB34067; ECs_0644 |
GeneIDi | 58462627 917003 |
KEGGi | ece:Z0749 ecs:ECs0644 |
PATRICi | fig|386585.9.peg.755 |
Phylogenomic databases
eggNOGi | COG0450, Bacteria |
HOGENOMi | CLU_042529_21_3_6 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR017559, AhpC IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
PANTHERi | PTHR10681:SF121, PTHR10681:SF121, 1 hit |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR03137, AhpC, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AHPC_ECO57 | |
Accessioni | P0AE10Primary (citable) accession number: P0AE10 Secondary accession number(s): P26427 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 98 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families