UniProtKB - P0ADX9 (RSMD_ECOLI)
Protein
Ribosomal RNA small subunit methyltransferase D
Gene
rsmD
Organism
Escherichia coli (strain K12)
Status
Functioni
Specifically methylates the guanine in position 966 of 16S rRNA in the assembled 30S particle.1 Publication
Catalytic activityi
- guanosine966 in 16S rRNA + S-adenosyl-L-methionine = H+ + N2-methylguanosine966 in 16S rRNA + S-adenosyl-L-homocysteineEC:2.1.1.171
GO - Molecular functioni
- 16S rRNA (guanine(966)-N(2))-methyltransferase activity Source: EcoCyc
- nucleic acid binding Source: InterPro
GO - Biological processi
- rRNA base methylation Source: EcoCyc
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | rRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10343-MONOMER MetaCyc:EG10343-MONOMER |
BRENDAi | 2.1.1.171, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal RNA small subunit methyltransferase D (EC:2.1.1.171)Alternative name(s): 16S rRNA m2G966 methyltransferase rRNA (guanine-N(2)-)-methyltransferase |
Gene namesi | Name:rsmD Synonyms:yhhF Ordered Locus Names:b3465, JW3430 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169548 | 1 – 198 | Ribosomal RNA small subunit methyltransferase DAdd BLAST | 198 |
Proteomic databases
jPOSTi | P0ADX9 |
PaxDbi | P0ADX9 |
PRIDEi | P0ADX9 |
2D gel databases
SWISS-2DPAGEi | P0ADX9 |
Interactioni
Binary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 4262500, 62 interactors |
DIPi | DIP-48275N |
IntActi | P0ADX9, 6 interactors |
STRINGi | 511145.b3465 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0ADX9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ADX9 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0742, Bacteria |
HOGENOMi | CLU_075826_2_2_6 |
InParanoidi | P0ADX9 |
PhylomeDBi | P0ADX9 |
Family and domain databases
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR004398, RNA_MeTrfase_RsmD IPR029063, SAM-dependent_MTases |
PIRSFi | PIRSF004553, CHP00095, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00095, TIGR00095, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ADX9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKPNHSGSG QIRIIGGQWR GRKLPVPDSP GLRPTTDRVR ETLFNWLAPV
60 70 80 90 100
IVDAQCLDCF AGSGALGLEA LSRYAAGATL IEMDRAVSQQ LIKNLATLKA
110 120 130 140 150
GNARVVNSNA MSFLAQKGTP HNIVFVDPPF RRGLLEETIN LLEDNGWLAD
160 170 180 190
EALIYVESEV ENGLPTVPAN WSLHREKVAG QVAYRLYQRE AQGESDAD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04398 Genomic DNA Translation: CAA27983.1 U00039 Genomic DNA Translation: AAB18440.1 U00096 Genomic DNA Translation: AAC76490.1 AP009048 Genomic DNA Translation: BAE77828.1 |
PIRi | S03129, QQECX3 |
RefSeqi | NP_417922.1, NC_000913.3 WP_000743193.1, NZ_STEB01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC76490; AAC76490; b3465 BAE77828; BAE77828; BAE77828 |
GeneIDi | 58462550 947977 |
KEGGi | ecj:JW3430 eco:b3465 |
PATRICi | fig|511145.12.peg.3564 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04398 Genomic DNA Translation: CAA27983.1 U00039 Genomic DNA Translation: AAB18440.1 U00096 Genomic DNA Translation: AAC76490.1 AP009048 Genomic DNA Translation: BAE77828.1 |
PIRi | S03129, QQECX3 |
RefSeqi | NP_417922.1, NC_000913.3 WP_000743193.1, NZ_STEB01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2FPO | X-ray | 2.05 | A/B/C/D/E/F | 1-198 | [»] | |
SMRi | P0ADX9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262500, 62 interactors |
DIPi | DIP-48275N |
IntActi | P0ADX9, 6 interactors |
STRINGi | 511145.b3465 |
2D gel databases
SWISS-2DPAGEi | P0ADX9 |
Proteomic databases
jPOSTi | P0ADX9 |
PaxDbi | P0ADX9 |
PRIDEi | P0ADX9 |
Genome annotation databases
EnsemblBacteriai | AAC76490; AAC76490; b3465 BAE77828; BAE77828; BAE77828 |
GeneIDi | 58462550 947977 |
KEGGi | ecj:JW3430 eco:b3465 |
PATRICi | fig|511145.12.peg.3564 |
Organism-specific databases
EchoBASEi | EB0339 |
Phylogenomic databases
eggNOGi | COG0742, Bacteria |
HOGENOMi | CLU_075826_2_2_6 |
InParanoidi | P0ADX9 |
PhylomeDBi | P0ADX9 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10343-MONOMER MetaCyc:EG10343-MONOMER |
BRENDAi | 2.1.1.171, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0ADX9 |
PROi | PR:P0ADX9 |
Family and domain databases
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR004398, RNA_MeTrfase_RsmD IPR029063, SAM-dependent_MTases |
PIRSFi | PIRSF004553, CHP00095, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00095, TIGR00095, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RSMD_ECOLI | |
Accessioni | P0ADX9Primary (citable) accession number: P0ADX9 Secondary accession number(s): P10120, Q2M7C8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | April 7, 2021 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families