UniProtKB - P0ADI4 (ENTB_ECOLI)
Enterobactin synthase component B
entB
Functioni
Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. The serine trilactone serves as a scaffolding for the three catechol functionalities that provide hexadentate coordination for the tightly ligated iron2+ atoms. EntB is a bifunctional protein that serves as an isochorismate lyase and an aryl carrier protein (ArCP). Catalyzes the conversion of isochorismate to 2,3-dihydro-2,3-dihydroxybenzoate (2,3-diDHB), the precursor of 2,3-dihydroxybenzoate (DHB). In the enterobactin assembly, EntB functions as an aryl carrier protein phosphopantetheinylated near the C terminus by EntD to yield holo-EntB, which is then acylated by EntE with 2,3-dihydroxybenzoyl-AMP to form DHB-holo-EntB. Then this product will serve in the formation of the amide bond between 2,3-dihydroxybenzoate (DHB) and L-serine.
8 PublicationsCatalytic activityi
- 3 2,3-dihydroxybenzoate + 6 ATP + 3 L-serine = 6 AMP + 6 diphosphate + enterobactin + 4 H+4 PublicationsEC:6.3.2.144 Publications
- EC:3.3.2.14 Publications
Cofactori
Activity regulationi
Kineticsi
- KM=14.7 µM for isochorismate (at pH 7 and 37 degrees Celsius)1 Publication
- KM=23 µM for 4,5-dihydroisochorismate (at pH 7 and 37 degrees Celsius)1 Publication
- KM=86 µM for 3-[(carboxyethenyl)oxy]-1-cyclohexene-1-carboxylic acid (at pH 7 and 37 degrees Celsius)1 Publication
- KM=120 µM for 3-[(1-carboxylatoethenyl)oxy]-cyclohepta-1,6-diene-1-carboxylate (at pH 7 and 37 degrees Celsius)1 Publication
- KM=280 µM for cis-3-[(carboxyethenyl)oxy]-4-cyclohexene-1-carboxylic acid (at pH 7 and 37 degrees Celsius)1 Publication
- Vmax=18.5 µmol/min/mg enzyme (at pH 7 and 37 degrees Celsius)1 Publication
pH dependencei
: enterobactin biosynthesis Pathwayi
This protein is involved in the pathway enterobactin biosynthesis, which is part of Siderophore biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway enterobactin biosynthesis and in Siderophore biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 227 | Magnesium1 Publication | 1 | |
Metal bindingi | 242 | Magnesium; via carbonyl oxygen1 Publication | 1 | |
Metal bindingi | 244 | Magnesium1 Publication | 1 |
GO - Molecular functioni
- 2,3-dihydroxybenzoate-serine ligase activity Source: UniProtKB
- identical protein binding Source: IntAct
- isochorismatase activity Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- phosphopantetheine binding Source: UniProtKB
- transferase activity, transferring alkyl or aryl (other than methyl) groups Source: EcoliWiki
GO - Biological processi
- enterobactin biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Ligase, Multifunctional enzyme |
Biological process | Enterobactin biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:ENTB-MONOMER |
BRENDAi | 3.3.2.1, 2026 6.3.2.14, 2026 |
SABIO-RKi | P0ADI4 |
UniPathwayi | UPA00017 |
Names & Taxonomyi
Protein namesi | Recommended name: Enterobactin synthase component B1 Publication (EC:6.3.2.144 Publications)Alternative name(s): Enterobactin biosynthesis bifunctional protein EntB1 Publication Enterochelin synthase B1 Publication Including the following 2 domains: Alternative name(s): 2,3-dihydro-2,3-dihydroxybenzoate synthase1 Publication Isochorismate lyase1 Publication Aryl carrier protein1 Publication Short name: ArCP1 Publication |
Gene namesi | Name:entB1 Publication Synonyms:entG1 Publication Ordered Locus Names:b0595, JW0587 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: EcoCyc
Plasma Membrane
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 240 | D → R: The catalytic efficiency slightly increases, but a 8-fold decrease of the affinity for the S-dihydroxybenzoyltransferase EntE is observed. 1 Publication | 1 | |
Mutagenesisi | 242 | G → A: Not efficiently phosphopantetheinylated by EntD. 1 Publication | 1 | |
Mutagenesisi | 244 | D → A: Efficiently phosphopantetheinylated by EntD. 1 Publication | 1 | |
Mutagenesisi | 244 | D → R: Not efficiently phosphopantetheinylated by EntD. 1 Publication | 1 | |
Mutagenesisi | 249 | M → A: Unable to recognize EntE and EntF. Exhibits a decrease in the enterobactin production compared to the wild-type. Still able to be phosphopantetheinylated. 1 Publication | 1 | |
Mutagenesisi | 263 | D → R: The catalytic efficiency is the same as the wild-type, but a 3-fold decrease of the affinity for the S-dihydroxybenzoyltransferase EntE is observed. 1 Publication | 1 | |
Mutagenesisi | 264 | F → E: The affinity for EntE and catalytic efficiency of the S-dihydroxybenzoyltransferase EntE are below that of the wild-type. 2 Publications | 1 | |
Mutagenesisi | 268 | A → Q: Unable to recognize EntE and EntF. Exhibits a decrease in the enterobactin production compared to the wild-type. Still able to be phosphopantetheinylated. 1 Publication | 1 | |
Mutagenesisi | 269 | K → A: Behaves similarly to the wild-type. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000201822 | 2 – 285 | Enterobactin synthase component BAdd BLAST | 284 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 245 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphopantetheine, PhosphoproteinProteomic databases
jPOSTi | P0ADI4 |
PaxDbi | P0ADI4 |
PRIDEi | P0ADI4 |
2D gel databases
SWISS-2DPAGEi | P0ADI4 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Proteins EntB, EntD, EntE, and EntF form a multienzyme complex called enterobactin synthase. Dimer.
4 PublicationsBinary interactionsi
P0ADI4
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-547993,EBI-547993 |
entE [P10378] | 3 | EBI-547993,EBI-550322 |
entH [P0A8Y8] | 3 | EBI-547993,EBI-1118982 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4260905, 201 interactors 850538, 5 interactors |
ComplexPortali | CPX-5748, entBE aryl carrier complex |
DIPi | DIP-9512N |
IntActi | P0ADI4, 29 interactors |
STRINGi | 511145.b0595 |
Structurei
Secondary structure
3D structure databases
SMRi | P0ADI4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ADI4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 209 – 284 | CarrierPROSITE-ProRule annotationAdd BLAST | 76 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 213 | Isochorismatase1 PublicationAdd BLAST | 212 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1535, Bacteria COG3433, Bacteria |
HOGENOMi | CLU_068979_2_0_6 |
InParanoidi | P0ADI4 |
PhylomeDBi | P0ADI4 |
Family and domain databases
Gene3Di | 1.10.1200.10, 1 hit 3.40.50.850, 1 hit |
InterProi | View protein in InterPro IPR036736, ACP-like_sf IPR016291, Isochorismatase IPR000868, Isochorismatase-like IPR036380, Isochorismatase-like_sf IPR009081, PP-bd_ACP |
Pfami | View protein in Pfam PF00857, Isochorismatase, 1 hit PF00550, PP-binding, 1 hit |
PIRSFi | PIRSF001111, Isochorismatase, 1 hit |
PRINTSi | PR01398, ISCHRISMTASE |
SUPFAMi | SSF47336, SSF47336, 1 hit SSF52499, SSF52499, 1 hit |
PROSITEi | View protein in PROSITE PS50075, CARRIER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MAIPKLQAYA LPESHDIPQN KVDWAFEPQR AALLIHDMQD YFVSFWGENC
60 70 80 90 100
PMMEQVIANI AALRDYCKQH NIPVYYTAQP KEQSDEDRAL LNDMWGPGLT
110 120 130 140 150
RSPEQQKVVD RLTPDADDTV LVKWRYSAFH RSPLEQMLKE SGRNQLIITG
160 170 180 190 200
VYAHIGCMTT ATDAFMRDIK PFMVADALAD FSRDEHLMSL KYVAGRSGRV
210 220 230 240 250
VMTEELLPAP IPASKAALRE VILPLLDESD EPFDDDNLID YGLDSVRMMA
260 270 280
LAARWRKVHG DIDFVMLAKN PTIDAWWKLL SREVK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24148 Unassigned DNA Translation: AAA16102.1 M24143 Genomic DNA Translation: AAA76835.1 U82598 Genomic DNA Translation: AAB40795.1 U00096 Genomic DNA Translation: AAC73696.1 AP009048 Genomic DNA Translation: BAE76350.1 |
PIRi | C91904, YXECIC |
RefSeqi | NP_415127.1, NC_000913.3 WP_001007138.1, NZ_SSUV01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC73696; AAC73696; b0595 BAE76350; BAE76350; BAE76350 |
GeneIDi | 946178 |
KEGGi | ecj:JW0587 eco:b0595 |
PATRICi | fig|1411691.4.peg.1674 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24148 Unassigned DNA Translation: AAA16102.1 M24143 Genomic DNA Translation: AAA76835.1 U82598 Genomic DNA Translation: AAB40795.1 U00096 Genomic DNA Translation: AAC73696.1 AP009048 Genomic DNA Translation: BAE76350.1 |
PIRi | C91904, YXECIC |
RefSeqi | NP_415127.1, NC_000913.3 WP_001007138.1, NZ_SSUV01000008.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2FQ1 | X-ray | 2.30 | A/B | 1-285 | [»] | |
3RG2 | X-ray | 3.10 | A/B/C/D/E/F/G/H/I/J | 209-285 | [»] | |
4IZ6 | X-ray | 2.40 | A/B | 211-285 | [»] | |
SMRi | P0ADI4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260905, 201 interactors 850538, 5 interactors |
ComplexPortali | CPX-5748, entBE aryl carrier complex |
DIPi | DIP-9512N |
IntActi | P0ADI4, 29 interactors |
STRINGi | 511145.b0595 |
2D gel databases
SWISS-2DPAGEi | P0ADI4 |
Proteomic databases
jPOSTi | P0ADI4 |
PaxDbi | P0ADI4 |
PRIDEi | P0ADI4 |
Genome annotation databases
EnsemblBacteriai | AAC73696; AAC73696; b0595 BAE76350; BAE76350; BAE76350 |
GeneIDi | 946178 |
KEGGi | ecj:JW0587 eco:b0595 |
PATRICi | fig|1411691.4.peg.1674 |
Organism-specific databases
EchoBASEi | EB0256 |
Phylogenomic databases
eggNOGi | COG1535, Bacteria COG3433, Bacteria |
HOGENOMi | CLU_068979_2_0_6 |
InParanoidi | P0ADI4 |
PhylomeDBi | P0ADI4 |
Enzyme and pathway databases
UniPathwayi | UPA00017 |
BioCyci | EcoCyc:ENTB-MONOMER |
BRENDAi | 3.3.2.1, 2026 6.3.2.14, 2026 |
SABIO-RKi | P0ADI4 |
Miscellaneous databases
EvolutionaryTracei | P0ADI4 |
PROi | PR:P0ADI4 |
Family and domain databases
Gene3Di | 1.10.1200.10, 1 hit 3.40.50.850, 1 hit |
InterProi | View protein in InterPro IPR036736, ACP-like_sf IPR016291, Isochorismatase IPR000868, Isochorismatase-like IPR036380, Isochorismatase-like_sf IPR009081, PP-bd_ACP |
Pfami | View protein in Pfam PF00857, Isochorismatase, 1 hit PF00550, PP-binding, 1 hit |
PIRSFi | PIRSF001111, Isochorismatase, 1 hit |
PRINTSi | PR01398, ISCHRISMTASE |
SUPFAMi | SSF47336, SSF47336, 1 hit SSF52499, SSF52499, 1 hit |
PROSITEi | View protein in PROSITE PS50075, CARRIER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ENTB_ECOLI | |
Accessioni | P0ADI4Primary (citable) accession number: P0ADI4 Secondary accession number(s): P15048, Q2MBK6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | February 23, 2022 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families