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Protein

Acetolactate synthase isozyme 2 small subunit

Gene

ilvM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

E.coli contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.

Catalytic activityi

2 pyruvate = 2-acetolactate + CO2.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
  • thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase isozyme 1 small subunit (ilvN), Putative acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 large subunit (ilvB), Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase isozyme 3 small subunit (ilvH)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase isozyme 1 small subunit (ilvN), Putative acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 large subunit (ilvB), Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase isozyme 3 small subunit (ilvH)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • acetolactate synthase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
LigandMagnesium, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciEcoCyc:SMALLILVM-MONOMER
MetaCyc:SMALLILVM-MONOMER
SABIO-RKiP0ADG1
UniPathwayiUPA00047; UER00055
UPA00049; UER00059

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase isozyme 2 small subunit (EC:2.2.1.6)
Alternative name(s):
ALS-II
Acetohydroxy-acid synthase II small subunit
Short name:
AHAS-II
Gene namesi
Name:ilvM
Ordered Locus Names:b3769, JW3742
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10501 ilvM

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001514291 – 87Acetolactate synthase isozyme 2 small subunitAdd BLAST87

Proteomic databases

PaxDbiP0ADG1
PRIDEiP0ADG1

Interactioni

Subunit structurei

Tetramer of two large and two small chains.

Protein-protein interaction databases

BioGridi4263292, 7 interactors
IntActiP0ADG1, 3 interactors
STRINGi316385.ECDH10B_3958

Structurei

3D structure databases

ProteinModelPortaliP0ADG1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 78ACTPROSITE-ProRule annotationAdd BLAST74

Phylogenomic databases

eggNOGiENOG4105P3Y Bacteria
COG3978 LUCA
HOGENOMiHOG000286146
KOiK11258
OMAiTRHRGFQ

Family and domain databases

InterProiView protein in InterPro
IPR002912 ACT_dom
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit

Sequencei

Sequence statusi: Complete.

P0ADG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMQHQVNVSA RFNPETLERV LRVVRHRGFH VCSMNMAAAS DAQNINIELT
60 70 80
VASPRSVDLL FSQLNKLVDV AHVAICQSTT TSQQIRA
Length:87
Mass (Da):9,703
Last modified:December 4, 2007 - v2
Checksum:iCEB6B5211262E0AF
GO

Sequence cautioni

The sequence AAB59051 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA26261 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02413 Genomic DNA Translation: CAA26261.1 Different initiation.
M10313 Genomic DNA Translation: AAB59051.1 Different initiation.
X04890 Genomic DNA Translation: CAA28574.1
M87049 Genomic DNA Translation: AAA67572.1
U00096 Genomic DNA Translation: AAC77489.1
AP009048 Genomic DNA Translation: BAE77528.1
RefSeqiNP_418217.1, NC_000913.3
WP_000983255.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77489; AAC77489; b3769
BAE77528; BAE77528; BAE77528
GeneIDi948279
KEGGiecj:JW3742
eco:b3769
PATRICifig|1411691.4.peg.2937

Similar proteinsi

Entry informationi

Entry nameiILVM_ECOLI
AccessioniPrimary (citable) accession number: P0ADG1
Secondary accession number(s): P13048, P78269, Q2M878
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 4, 2007
Last modified: March 28, 2018
This is version 87 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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