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Protein

Acetolactate synthase isozyme 1 small subunit

Gene

ilvN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

E.coli contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.

Catalytic activityi

2 pyruvate = 2-acetolactate + CO2.

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 small subunit (ilvN), Acetolactate synthase isozyme 3 small subunit (ilvH), Acetolactate synthase isozyme 3 large subunit (ilvI), Putative acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 large subunit (ilvB)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 small subunit (ilvN), Acetolactate synthase isozyme 3 small subunit (ilvH), Acetolactate synthase isozyme 3 large subunit (ilvI), Putative acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 large subunit (ilvB)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • acetolactate synthase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:SMALLILVN-MONOMER
MetaCyc:SMALLILVN-MONOMER
UniPathwayi
UPA00047;UER00055

UPA00049;UER00059

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase isozyme 1 small subunit (EC:2.2.1.6)
Alternative name(s):
Acetohydroxy-acid synthase I small subunit
Short name:
AHAS-I
Short name:
ALS-I
Gene namesi
Name:ilvN
Ordered Locus Names:b3670, JW3645
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10502 ilvN

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001514191 – 96Acetolactate synthase isozyme 1 small subunitAdd BLAST96

Proteomic databases

PaxDbiP0ADF8
PRIDEiP0ADF8

Interactioni

Subunit structurei

Dimer of large and small chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
ilvBP081424EBI-1133508,EBI-552948

Protein-protein interaction databases

BioGridi4262585, 7 interactors
IntActiP0ADF8, 6 interactors
STRINGi316385.ECDH10B_3853

Structurei

Secondary structure

196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0ADF8
SMRiP0ADF8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 83ACTPROSITE-ProRule annotationAdd BLAST74

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105M71 Bacteria
COG0440 LUCA
HOGENOMiHOG000126293
KOiK01653
OMAiQSHENVI
PhylomeDBiP0ADF8

Family and domain databases

InterProiView protein in InterPro
IPR004789 Acetalactate_synth_ssu
IPR002912 ACT_dom
PANTHERiPTHR30239 PTHR30239, 1 hit
PfamiView protein in Pfam
PF01842 ACT, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit

Sequencei

Sequence statusi: Complete.

P0ADF8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQNTTHDNVI LELTVRNHPG VMTHVCGLFA RRAFNVEGIL CLPIQDSDKS
60 70 80 90
HIWLLVNDDQ RLEQMISQID KLEDVVKVQR NQSDPTMFNK IAVFFQ
Length:96
Mass (Da):11,106
Last modified:December 6, 2005 - v1
Checksum:i7F37DD421EDD3802
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24H → D (PubMed:2989782).Curated1
Sequence conflicti24H → D (PubMed:2989781).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02541 Genomic DNA Translation: CAA26388.1
L10328 Genomic DNA Translation: AAA62022.1
U00096 Genomic DNA Translation: AAC76693.1
AP009048 Genomic DNA Translation: BAE77623.1
M17102 Genomic DNA Translation: AAA24719.1
PIRiG65168 YCEC1S
RefSeqiNP_418126.1, NC_000913.3
WP_001181706.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76693; AAC76693; b3670
BAE77623; BAE77623; BAE77623
GeneIDi948183
KEGGiecj:JW3645
eco:b3670
PATRICifig|1411691.4.peg.3035

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02541 Genomic DNA Translation: CAA26388.1
L10328 Genomic DNA Translation: AAA62022.1
U00096 Genomic DNA Translation: AAC76693.1
AP009048 Genomic DNA Translation: BAE77623.1
M17102 Genomic DNA Translation: AAA24719.1
PIRiG65168 YCEC1S
RefSeqiNP_418126.1, NC_000913.3
WP_001181706.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LVWNMR-A/B1-96[»]
ProteinModelPortaliP0ADF8
SMRiP0ADF8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262585, 7 interactors
IntActiP0ADF8, 6 interactors
STRINGi316385.ECDH10B_3853

Proteomic databases

PaxDbiP0ADF8
PRIDEiP0ADF8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76693; AAC76693; b3670
BAE77623; BAE77623; BAE77623
GeneIDi948183
KEGGiecj:JW3645
eco:b3670
PATRICifig|1411691.4.peg.3035

Organism-specific databases

EchoBASEiEB0497
EcoGeneiEG10502 ilvN

Phylogenomic databases

eggNOGiENOG4105M71 Bacteria
COG0440 LUCA
HOGENOMiHOG000126293
KOiK01653
OMAiQSHENVI
PhylomeDBiP0ADF8

Enzyme and pathway databases

UniPathwayi
UPA00047;UER00055

UPA00049;UER00059

BioCyciEcoCyc:SMALLILVN-MONOMER
MetaCyc:SMALLILVN-MONOMER

Miscellaneous databases

PROiPR:P0ADF8

Family and domain databases

InterProiView protein in InterPro
IPR004789 Acetalactate_synth_ssu
IPR002912 ACT_dom
PANTHERiPTHR30239 PTHR30239, 1 hit
PfamiView protein in Pfam
PF01842 ACT, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiILVN_ECOLI
AccessioniPrimary (citable) accession number: P0ADF8
Secondary accession number(s): P08143, Q2M7Y3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: December 6, 2005
Last modified: October 10, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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