UniProtKB - P0ADF8 (ILVN_ECOLI)
Protein
Acetolactate synthase isozyme 1 small subunit
Gene
ilvN
Organism
Escherichia coli (strain K12)
Status
Functioni
Miscellaneous
E.coli contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.
Catalytic activityi
- EC:2.2.1.6
: L-isoleucine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- Acetolactate synthase isozyme 1 small subunit (ilvN), Putative acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 large subunit (ilvB), Acetolactate synthase (ilvB), Acetolactate synthase (FAZ83_14160), Acetolactate synthase (ilvI), Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase (ilvN), Acetolactate synthase isozyme 3 small subunit (ilvH), Acetolactate synthase (ilvN)
- Ketol-acid reductoisomerase (NADP(+)) (ilvC), Ketol-acid reductoisomerase (NADP(+)) (ilvC)
- Dihydroxy-acid dehydratase (ilvD), Dihydroxy-acid dehydratase (ilvD)
- Branched-chain-amino-acid aminotransferase (ilvE), Branched-chain-amino-acid aminotransferase (ilvE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.
Pathwayi: L-valine biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes L-valine from pyruvate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- Acetolactate synthase isozyme 1 small subunit (ilvN), Putative acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 large subunit (ilvB), Acetolactate synthase (ilvB), Acetolactate synthase (FAZ83_14160), Acetolactate synthase (ilvI), Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase (ilvN), Acetolactate synthase isozyme 3 small subunit (ilvH), Acetolactate synthase (ilvN)
- Ketol-acid reductoisomerase (NADP(+)) (ilvC), Ketol-acid reductoisomerase (NADP(+)) (ilvC)
- Dihydroxy-acid dehydratase (ilvD), Dihydroxy-acid dehydratase (ilvD)
- Branched-chain-amino-acid aminotransferase (ilvE), Branched-chain-amino-acid aminotransferase (ilvE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- acetolactate synthase activity Source: EcoCyc
- acetolactate synthase regulator activity Source: InterPro
GO - Biological processi
- isoleucine biosynthetic process Source: EcoCyc
- valine biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Transferase |
Biological process | Amino-acid biosynthesis, Branched-chain amino acid biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:SMALLILVN-MONOMER MetaCyc:SMALLILVN-MONOMER |
UniPathwayi | UPA00047;UER00055 UPA00049;UER00059 |
Names & Taxonomyi
Protein namesi | Recommended name: Acetolactate synthase isozyme 1 small subunit (EC:2.2.1.6)Alternative name(s): Acetohydroxy-acid synthase I small subunit Short name: AHAS-I Short name: ALS-I |
Gene namesi | Name:ilvN Ordered Locus Names:b3670, JW3645 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000151419 | 1 – 96 | Acetolactate synthase isozyme 1 small subunitAdd BLAST | 96 |
Proteomic databases
jPOSTi | P0ADF8 |
PaxDbi | P0ADF8 |
PRIDEi | P0ADF8 |
Interactioni
Subunit structurei
Dimer of large and small chains.
Binary interactionsi
P0ADF8
With | #Exp. | IntAct |
---|---|---|
ilvB [P08142] | 4 | EBI-1133508,EBI-552948 |
Protein-protein interaction databases
BioGRIDi | 4262585, 7 interactors 852489, 3 interactors |
ComplexPortali | CPX-3573, Acetolactate synthase I complex |
IntActi | P0ADF8, 6 interactors |
STRINGi | 511145.b3670 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0ADF8 |
SMRi | P0ADF8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 83 | ACTPROSITE-ProRule annotationAdd BLAST | 74 |
Sequence similaritiesi
Belongs to the acetolactate synthase small subunit family.Curated
Phylogenomic databases
eggNOGi | COG0440, Bacteria |
HOGENOMi | CLU_165363_0_0_6 |
PhylomeDBi | P0ADF8 |
Family and domain databases
CDDi | cd04878, ACT_AHAS, 1 hit |
InterProi | View protein in InterPro IPR004789, Acetalactate_synth_ssu IPR002912, ACT_dom IPR039557, AHAS_ACT |
PANTHERi | PTHR30239, PTHR30239, 1 hit |
Pfami | View protein in Pfam PF01842, ACT, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ADF8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQNTTHDNVI LELTVRNHPG VMTHVCGLFA RRAFNVEGIL CLPIQDSDKS
60 70 80 90
HIWLLVNDDQ RLEQMISQID KLEDVVKVQR NQSDPTMFNK IAVFFQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 24 | H → D (PubMed:2989782).Curated | 1 | |
Sequence conflicti | 24 | H → D (PubMed:2989781).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02541 Genomic DNA Translation: CAA26388.1 L10328 Genomic DNA Translation: AAA62022.1 U00096 Genomic DNA Translation: AAC76693.1 AP009048 Genomic DNA Translation: BAE77623.1 M17102 Genomic DNA Translation: AAA24719.1 |
PIRi | G65168, YCEC1S |
RefSeqi | NP_418126.1, NC_000913.3 WP_001181706.1, NZ_STEB01000015.1 |
Genome annotation databases
EnsemblBacteriai | AAC76693; AAC76693; b3670 BAE77623; BAE77623; BAE77623 |
GeneIDi | 56816289 948183 |
KEGGi | ecj:JW3645 eco:b3670 |
PATRICi | fig|1411691.4.peg.3035 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02541 Genomic DNA Translation: CAA26388.1 L10328 Genomic DNA Translation: AAA62022.1 U00096 Genomic DNA Translation: AAC76693.1 AP009048 Genomic DNA Translation: BAE77623.1 M17102 Genomic DNA Translation: AAA24719.1 |
PIRi | G65168, YCEC1S |
RefSeqi | NP_418126.1, NC_000913.3 WP_001181706.1, NZ_STEB01000015.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LVW | NMR | - | A/B | 1-96 | [»] | |
5YPP | X-ray | 1.90 | A/B/C/D/E/F | 1-96 | [»] | |
5YPY | X-ray | 1.97 | A/B/C/D | 1-96 | [»] | |
5YUM | X-ray | 2.43 | A | 1-96 | [»] | |
BMRBi | P0ADF8 | |||||
SMRi | P0ADF8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262585, 7 interactors 852489, 3 interactors |
ComplexPortali | CPX-3573, Acetolactate synthase I complex |
IntActi | P0ADF8, 6 interactors |
STRINGi | 511145.b3670 |
Proteomic databases
jPOSTi | P0ADF8 |
PaxDbi | P0ADF8 |
PRIDEi | P0ADF8 |
Genome annotation databases
EnsemblBacteriai | AAC76693; AAC76693; b3670 BAE77623; BAE77623; BAE77623 |
GeneIDi | 56816289 948183 |
KEGGi | ecj:JW3645 eco:b3670 |
PATRICi | fig|1411691.4.peg.3035 |
Organism-specific databases
EchoBASEi | EB0497 |
Phylogenomic databases
eggNOGi | COG0440, Bacteria |
HOGENOMi | CLU_165363_0_0_6 |
PhylomeDBi | P0ADF8 |
Enzyme and pathway databases
UniPathwayi | UPA00047;UER00055 UPA00049;UER00059 |
BioCyci | EcoCyc:SMALLILVN-MONOMER MetaCyc:SMALLILVN-MONOMER |
Miscellaneous databases
PROi | PR:P0ADF8 |
Family and domain databases
CDDi | cd04878, ACT_AHAS, 1 hit |
InterProi | View protein in InterPro IPR004789, Acetalactate_synth_ssu IPR002912, ACT_dom IPR039557, AHAS_ACT |
PANTHERi | PTHR30239, PTHR30239, 1 hit |
Pfami | View protein in Pfam PF01842, ACT, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ILVN_ECOLI | |
Accessioni | P0ADF8Primary (citable) accession number: P0ADF8 Secondary accession number(s): P08143, Q2M7Y3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | December 6, 2005 | |
Last modified: | February 10, 2021 | |
This is version 112 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families