ilvN

Functionⁱ

Miscellaneous

E.coli contains genes for 3 AHAS isozymes: ilvBN, ilvGM and ilvIH.

Catalytic activityⁱ

2 pyruvate = 2-acetolactate + CO₂.

Pathwayⁱ: L-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:

Acetolactate synthase isozyme 1 small subunit (ilvN), Putative acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 1 large subunit (ilvB), Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase isozyme 3 small subunit (ilvH)
Ketol-acid reductoisomerase (NADP(+)) (ilvC)
Dihydroxy-acid dehydratase (ilvD)
Branched-chain-amino-acid aminotransferase (ilvE)

This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayⁱ: L-valine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:

This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functionⁱ

acetolactate synthase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 16326011

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

isoleucine biosynthetic process
Source: EcoCyc
Inferred from direct assayⁱ
- 370104
valine biosynthetic process
Source: EcoCyc
Inferred from direct assayⁱ
- 16326011

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Enzyme and pathway databases

BioCycⁱ	EcoCyc:SMALLILVN-MONOMER MetaCyc:SMALLILVN-MONOMER
UniPathwayⁱ	UPA00047; UER00055 UPA00049; UER00059

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Acetolactate synthase isozyme 1 small subunit (EC:2.2.1.6) Alternative name(s): Acetohydroxy-acid synthase I small subunit Short name: AHAS-I Short name: ALS-I
Gene namesⁱ	Name:ilvN Ordered Locus Names:b3670, JW3645
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10502 ilvN

EcoGeneⁱ

EG10502 ilvN

Subcellular locationⁱ

GO - Cellular componentⁱ

acetolactate synthase complex
Source: EcoCyc
Inferred from direct assayⁱ
- 6360995
cytosol Source: GO_Central

View the complete GO annotation on QuickGO ...

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000151419	1 – 96	Acetolactate synthase isozyme 1 small subunitAdd BLAST		96

Proteomic databases

PaxDbⁱ	P0ADF8
PRIDEⁱ	P0ADF8

Interactionⁱ

Subunit structureⁱ

Dimer of large and small chains.

Protein-protein interaction databases

BioGridⁱ	4262585, 7 interactors
IntActⁱ	P0ADF8, 6 interactors
STRINGⁱ	316385.ECDH10B_3853

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	7 – 16	Combined sources	10
Helixⁱ	22 – 32	Combined sources	11
Beta strandⁱ	37 – 44	Combined sources	8
Turnⁱ	45 – 48	Combined sources	4
Beta strandⁱ	49 – 57	Combined sources	9
Helixⁱ	62 – 70	Combined sources	9
Beta strandⁱ	75 – 81	Combined sources	7
Helixⁱ	86 – 94	Combined sources	9

3D structure databases

ProteinModelPortalⁱ	P0ADF8
SMRⁱ	P0ADF8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	10 – 83	ACTPROSITE-ProRule annotationAdd BLAST		74

Sequence similaritiesⁱ

Belongs to the acetolactate synthase small subunit family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105M71 Bacteria COG0440 LUCA
HOGENOMⁱ	HOG000126293
KEGG Orthology (KO) More...KOⁱ	K01653
OMAⁱ	QSHENVI
PhylomeDBⁱ	P0ADF8

Family and domain databases

InterProⁱ	View protein in InterPro IPR004789 Acetalactate_synth_ssu IPR002912 ACT_dom
PANTHERⁱ	PTHR30239 PTHR30239, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01842 ACT, 1 hit
PROSITEⁱ	View protein in PROSITE PS51671 ACT, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0ADF8-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MQNTTHDNVI LELTVRNHPG VMTHVCGLFA RRAFNVEGIL CLPIQDSDKS 
        60         70         80         90 
HIWLLVNDDQ RLEQMISQID KLEDVVKVQR NQSDPTMFNK IAVFFQ

Length:96

Mass (Da):11,106

Last modified:December 6, 2005 - v1

Checksum:ⁱ7F37DD421EDD3802

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	24	H → D (PubMed:2989782).Curated		1
Sequence conflictⁱ	24	H → D (PubMed:2989781).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X02541 Genomic DNA Translation: CAA26388.1 L10328 Genomic DNA Translation: AAA62022.1 U00096 Genomic DNA Translation: AAC76693.1 AP009048 Genomic DNA Translation: BAE77623.1 M17102 Genomic DNA Translation: AAA24719.1
PIRⁱ	G65168 YCEC1S
NCBI Reference Sequences More...RefSeqⁱ	NP_418126.1, NC_000913.3 WP_001181706.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC76693; AAC76693; b3670 BAE77623; BAE77623; BAE77623
GeneIDⁱ	948183
KEGGⁱ	ecj:JW3645 eco:b3670
PATRICⁱ	fig\|1411691.4.peg.3035

Protein	Similar proteins		Species	Score	Length
P0ADF8	Acetolactate synthase isozyme 1 small subunit		ECO57		96
Acetolactate synthase isozyme 1 small subunit		ECOL6		96
Acetolactate synthase small subunit		Klebsiella pneumoniae IS22		96
Acetolactate synthase 1 regulatory subunit		Escherichia coli O127:H6		96
Valine-sensitive acetolactate synthase I small subunit		Escherichia coli D6-113.11		96
+159

Protein	Similar proteins		Species	Score	Length
P0ADF8	Acetolactate synthase isozyme 1 small subunit		ECO57		96
Acetolactate synthase isozyme 1 small subunit		ECOL6		96
Acetolactate synthase small subunit		Klebsiella pneumoniae IS22		96
Acetolactate synthase 1 regulatory subunit		Escherichia coli O127:H6		96
Valine-sensitive acetolactate synthase I small subunit		Escherichia coli D6-113.11		96
+203

Protein	Similar proteins		Species	Score	Length
P0ADF8	Acetolactate synthase isozyme 1 small subunit		ECO57		96
Acetolactate synthase isozyme 1 small subunit		ECOL6		96
Acetolactate synthase small subunit		Klebsiella pneumoniae IS22		96
Acetolactate synthase 1 regulatory subunit		Escherichia coli O127:H6		96
Valine-sensitive acetolactate synthase I small subunit		Escherichia coli D6-113.11		96
+203

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X02541 Genomic DNA Translation: CAA26388.1 L10328 Genomic DNA Translation: AAA62022.1 U00096 Genomic DNA Translation: AAC76693.1 AP009048 Genomic DNA Translation: BAE77623.1 M17102 Genomic DNA Translation: AAA24719.1
PIRⁱ	G65168 YCEC1S
NCBI Reference Sequences More...RefSeqⁱ	NP_418126.1, NC_000913.3 WP_001181706.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2LVW	NMR	-	A/B	1-96	[»]
ProteinModelPortalⁱ	P0ADF8
SMRⁱ	P0ADF8
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4262585, 7 interactors
IntActⁱ	P0ADF8, 6 interactors
STRINGⁱ	316385.ECDH10B_3853

Proteomic databases

PaxDbⁱ	P0ADF8
PRIDEⁱ	P0ADF8

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76693; AAC76693; b3670 BAE77623; BAE77623; BAE77623
GeneIDⁱ	948183
KEGGⁱ	ecj:JW3645 eco:b3670
PATRICⁱ	fig\|1411691.4.peg.3035

Organism-specific databases

EchoBASEⁱ	EB0497
EcoGeneⁱ	EG10502 ilvN

Phylogenomic databases

eggNOGⁱ	ENOG4105M71 Bacteria COG0440 LUCA
HOGENOMⁱ	HOG000126293
KEGG Orthology (KO) More...KOⁱ	K01653
OMAⁱ	QSHENVI
PhylomeDBⁱ	P0ADF8

Enzyme and pathway databases

UniPathwayⁱ	UPA00047; UER00055 UPA00049; UER00059
BioCycⁱ	EcoCyc:SMALLILVN-MONOMER MetaCyc:SMALLILVN-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P0ADF8

PROⁱ

PR:P0ADF8

Family and domain databases

InterProⁱ	View protein in InterPro IPR004789 Acetalactate_synth_ssu IPR002912 ACT_dom
PANTHERⁱ	PTHR30239 PTHR30239, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01842 ACT, 1 hit
PROSITEⁱ	View protein in PROSITE PS51671 ACT, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ILVN_ECOLI
Accessionⁱ	P0ADF8Primary (citable) accession number: P0ADF8 Secondary accession number(s): P08143, Q2M7Y3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
	Last sequence update:	December 6, 2005
	Last modified:	March 28, 2018
	This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0ADF8 (ILVN_ECOLI)

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Acetolactate synthase isozyme 1 small subunit

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-isoleucine biosynthesis

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-valine biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

Pathwayⁱ: L-isoleucine biosynthesis

Pathwayⁱ: L-valine biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ