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UniProtKB - P0ADA3 (NLPD_ECOLI)
Protein
Murein hydrolase activator NlpD
Gene
nlpD
Organism
Escherichia coli (strain K12)
Status
Functioni
Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.
2 PublicationsGO - Molecular functioni
- metalloendopeptidase activity Source: GO_Central
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- positive regulation of hydrolase activity Source: EcoCyc
- septum digestion after cytokinesis Source: EcoliWiki
Keywordsi
Biological process | Cell cycle, Cell division |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12111-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Murein hydrolase activator NlpD |
Gene namesi | Name:nlpD Ordered Locus Names:b2742, JW2712 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane Curated; Lipid-anchor PROSITE-ProRule annotation
Note: Localizes at the septal ring.1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cell division site Source: EcoliWiki
- cell outer membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Cells are shorter in a single mutant, double envC-nlpD disruptions have defects in septation and cell separation and form long filaments (8-fold longer). Cell length increase is more exacerbated with a triple mepM (yebA) or ygeR disruption (15-fold longer) and further yet by the quadruple disruption mutant (envC-nlpD-mepM(yebA)-ygeR, over 21-fold longer). Quadruple mutants are less sensitive to ampicillin lysis.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Add BLAST | 25 | |
ChainiPRO_0000018029 | 26 – 379 | Murein hydrolase activator NlpDAdd BLAST | 354 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 26 | N-palmitoyl cysteinePROSITE-ProRule annotation1 Publication | 1 | |
Lipidationi | 26 | S-diacylglycerol cysteine1 Publication | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
jPOSTi | P0ADA3 |
PaxDbi | P0ADA3 |
PRIDEi | P0ADA3 |
2D gel databases
SWISS-2DPAGEi | P0ADA3 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261894, 278 interactors |
DIPi | DIP-48067N |
IntActi | P0ADA3, 4 interactors |
STRINGi | 511145.b2742 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 66 – 73 | 1-1 | 8 | |
Repeati | 74 – 81 | 1-2; approximate | 8 | |
Repeati | 82 – 89 | 1-3 | 8 | |
Repeati | 90 – 97 | 1-4; approximate | 8 | |
Domaini | 121 – 165 | LysMPROSITE-ProRule annotationAdd BLAST | 45 | |
Repeati | 205 – 211 | 2-1 | 7 | |
Repeati | 227 – 233 | 2-2 | 7 | |
Repeati | 239 – 245 | 2-3 | 7 | |
Repeati | 246 – 252 | 2-4 | 7 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 30 – 67 | DisorderedSequence analysisAdd BLAST | 38 | |
Regioni | 66 – 97 | 4 X 8 AA tandem repeats of Q-Q-P-Q-I-Q-P-VAdd BLAST | 32 | |
Regioni | 205 – 252 | 4 X 7 AA approximate repeatsAdd BLAST | 48 | |
Regioni | 210 – 231 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 240 – 259 | DisorderedSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Belongs to the E.coli NlpD/Haemophilus LppB family.Curated
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | COG1388, Bacteria COG4942, Bacteria |
HOGENOMi | CLU_029425_0_1_6 |
InParanoidi | P0ADA3 |
PhylomeDBi | P0ADA3 |
Family and domain databases
CDDi | cd00118, LysM, 1 hit |
Gene3Di | 2.70.70.10, 1 hit 3.10.350.10, 1 hit |
InterProi | View protein in InterPro IPR011055, Dup_hybrid_motif IPR018392, LysM_dom IPR036779, LysM_dom_sf IPR016047, Peptidase_M23 |
Pfami | View protein in Pfam PF01476, LysM, 1 hit PF01551, Peptidase_M23, 1 hit |
SMARTi | View protein in SMART SM00257, LysM, 1 hit |
SUPFAMi | SSF51261, SSF51261, 1 hit |
PROSITEi | View protein in PROSITE PS51782, LYSM, 1 hit PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0ADA3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAGSPKFTV RRIAALSLVS LWLAGCSDTS NPPAPVSSVN GNAPANTNSG
60 70 80 90 100
MLITPPPKMG TTSTAQQPQI QPVQQPQIQA TQQPQIQPVQ PVAQQPVQME
110 120 130 140 150
NGRIVYNRQY GNIPKGSYSG STYTVKKGDT LFYIAWITGN DFRDLAQRNN
160 170 180 190 200
IQAPYALNVG QTLQVGNASG TPITGGNAIT QADAAEQGVV IKPAQNSTVA
210 220 230 240 250
VASQPTITYS ESSGEQSANK MLPNNKPTAT TVTAPVTVPT ASTTEPTVSS
260 270 280 290 300
TSTSTPISTW RWPTEGKVIE TFGASEGGNK GIDIAGSKGQ AIIATADGRV
310 320 330 340 350
VYAGNALRGY GNLIIIKHND DYLSAYAHND TMLVREQQEV KAGQKIATMG
360 370
STGTSSTRLH FEIRYKGKSV NPLRYLPQR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 139 | G → A in BAA04487 (PubMed:8208244).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07869 Unassigned DNA Translation: AAA17875.1 U29579 Genomic DNA Translation: AAA69252.1 U00096 Genomic DNA Translation: AAC75784.1 AP009048 Genomic DNA Translation: BAE76819.1 D17549 Genomic DNA Translation: BAA04487.1 |
PIRi | B55522 |
RefSeqi | NP_417222.1, NC_000913.3 WP_001272592.1, NZ_STEB01000027.1 |
Genome annotation databases
EnsemblBacteriai | AAC75784; AAC75784; b2742 BAE76819; BAE76819; BAE76819 |
GeneIDi | 66673384 947011 |
KEGGi | ecj:JW2712 eco:b2742 |
PATRICi | fig|1411691.4.peg.3998 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07869 Unassigned DNA Translation: AAA17875.1 U29579 Genomic DNA Translation: AAA69252.1 U00096 Genomic DNA Translation: AAC75784.1 AP009048 Genomic DNA Translation: BAE76819.1 D17549 Genomic DNA Translation: BAA04487.1 |
PIRi | B55522 |
RefSeqi | NP_417222.1, NC_000913.3 WP_001272592.1, NZ_STEB01000027.1 |
3D structure databases
SMRi | P0ADA3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261894, 278 interactors |
DIPi | DIP-48067N |
IntActi | P0ADA3, 4 interactors |
STRINGi | 511145.b2742 |
2D gel databases
SWISS-2DPAGEi | P0ADA3 |
Proteomic databases
jPOSTi | P0ADA3 |
PaxDbi | P0ADA3 |
PRIDEi | P0ADA3 |
Genome annotation databases
EnsemblBacteriai | AAC75784; AAC75784; b2742 BAE76819; BAE76819; BAE76819 |
GeneIDi | 66673384 947011 |
KEGGi | ecj:JW2712 eco:b2742 |
PATRICi | fig|1411691.4.peg.3998 |
Organism-specific databases
EchoBASEi | EB2034 |
Phylogenomic databases
eggNOGi | COG1388, Bacteria COG4942, Bacteria |
HOGENOMi | CLU_029425_0_1_6 |
InParanoidi | P0ADA3 |
PhylomeDBi | P0ADA3 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12111-MONOMER |
Miscellaneous databases
PROi | PR:P0ADA3 |
Family and domain databases
CDDi | cd00118, LysM, 1 hit |
Gene3Di | 2.70.70.10, 1 hit 3.10.350.10, 1 hit |
InterProi | View protein in InterPro IPR011055, Dup_hybrid_motif IPR018392, LysM_dom IPR036779, LysM_dom_sf IPR016047, Peptidase_M23 |
Pfami | View protein in Pfam PF01476, LysM, 1 hit PF01551, Peptidase_M23, 1 hit |
SMARTi | View protein in SMART SM00257, LysM, 1 hit |
SUPFAMi | SSF51261, SSF51261, 1 hit |
PROSITEi | View protein in PROSITE PS51782, LYSM, 1 hit PS51257, PROKAR_LIPOPROTEIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NLPD_ECOLI | |
Accessioni | P0ADA3Primary (citable) accession number: P0ADA3 Secondary accession number(s): P33648, Q2MA87 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | February 23, 2022 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families