UniProtKB - P0AD96 (LIVJ_ECOLI)

BLAST

>sp|P0AD96|LIVJ_ECOLI Leu/Ile/Val-binding protein OS=Escherichia coli (strain K12) OX=83333 GN=livJ PE=1 SV=1
MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQEFTGAEQAVADINAKG
GIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCSSSTQPASDIYEDEGILMI
TPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVKPQRIAIVHDKQQYGEGLARA
VQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENIDFVYYGGYHPEMGQILRQARAAG
LKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYDQVPANKPIVDAIKAKKQDPSGAFV
WTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDTVMGPLTWDEKGDLKGFEFGVFDWHAN
GTATDAK

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History
Entry version 101 (07 Nov 2018)
Sequence version 1 (06 Dec 2005)
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Protein

Leu/Ile/Val-binding protein

Gene

livJ

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

This protein is a component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids.

GO - Biological processⁱ

isoleucine transport
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 323236
leucine transport
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 323236
valine transport
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 323236

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Amino-acid transport, Transport

Biological process

Amino-acid transport, Transport

Enzyme and pathway databases

BioCycⁱ	EcoCyc:LIVJ-MONOMER MetaCyc:LIVJ-MONOMER

BioCycⁱ

EcoCyc:LIVJ-MONOMER
MetaCyc:LIVJ-MONOMER

Protein family/group databases

TCDBⁱ	3.A.1.4.1 the atp-binding cassette (abc) superfamily

TCDBⁱ

3.A.1.4.1 the atp-binding cassette (abc) superfamily

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Leu/Ile/Val-binding protein Short name: LIV-BP
Gene namesⁱ	Name:livJ Ordered Locus Names:b3460, JW3425
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10539 livJ

EcoGeneⁱ

EG10539 livJ

Subcellular locationⁱ

Periplasm

GO - Cellular componentⁱ

outer membrane-bounded periplasmic space
Source: EcoCyc
Inferred from direct assayⁱ
- 15911532

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Periplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 23	3 PublicationsAdd BLAST		23
ChainⁱPRO_0000017700	24 – 367	Leu/Ile/Val-binding proteinAdd BLAST		344

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Disulfide bondⁱ	76 ↔ 101

Keywords - PTMⁱ

Disulfide bond

Proteomic databases

EPDⁱ	P0AD96
PaxDbⁱ	P0AD96
PRIDEⁱ	P0AD96

2D gel databases

SWISS-2DPAGEⁱ	P0AD96

SWISS-2DPAGEⁱ

P0AD96

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4259328, 24 interactors
IntActⁱ	P0AD96, 4 interactors
STRINGⁱ	316385.ECDH10B_3634

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	26 – 32	Combined sources	7
Beta strandⁱ	34 – 36	Combined sources	3
Helixⁱ	39 – 58	Combined sources	20
Turnⁱ	59 – 61	Combined sources	3
Beta strandⁱ	67 – 73	Combined sources	7
Helixⁱ	78 – 90	Combined sources	13
Beta strandⁱ	95 – 97	Combined sources	3
Helixⁱ	102 – 115	Combined sources	14
Beta strandⁱ	118 – 123	Combined sources	6
Helixⁱ	127 – 130	Combined sources	4
Beta strandⁱ	135 – 141	Combined sources	7
Helixⁱ	144 – 146	Combined sources	3
Helixⁱ	147 – 157	Combined sources	11
Beta strandⁱ	162 – 168	Combined sources	7
Helixⁱ	172 – 187	Combined sources	16
Beta strandⁱ	192 – 197	Combined sources	6
Helixⁱ	206 – 214	Combined sources	9
Beta strandⁱ	219 – 224	Combined sources	6
Helixⁱ	226 – 238	Combined sources	13
Beta strandⁱ	244 – 247	Combined sources	4
Helixⁱ	249 – 251	Combined sources	3
Helixⁱ	254 – 260	Combined sources	7
Helixⁱ	261 – 264	Combined sources	4
Beta strandⁱ	268 – 271	Combined sources	4
Helixⁱ	275 – 277	Combined sources	3
Helixⁱ	279 – 281	Combined sources	3
Helixⁱ	282 – 290	Combined sources	9
Helixⁱ	298 – 314	Combined sources	17
Helixⁱ	320 – 329	Combined sources	10
Beta strandⁱ	332 – 334	Combined sources	3
Beta strandⁱ	337 – 339	Combined sources	3
Beta strandⁱ	347 – 349	Combined sources	3
Beta strandⁱ	353 – 357	Combined sources	5
Beta strandⁱ	363 – 365	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	P0AD96
SMRⁱ	P0AD96
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P0AD96

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the leucine-binding protein family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	ENOG4105CP9 Bacteria COG0683 LUCA
HOGENOMⁱ	HOG000130989
InParanoidⁱ	P0AD96
KEGG Orthology (KO) More...KOⁱ	K01999
PhylomeDBⁱ	P0AD96

Family and domain databases

InterProⁱ	View protein in InterPro IPR028081 Leu-bd IPR000709 Leu_Ile_Val-bd IPR028082 Peripla_BP_I
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF13458 Peripla_BP_6, 1 hit
PRINTSⁱ	PR00337 LEUILEVALBP
SUPFAMⁱ	SSF53822 SSF53822, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P0AD96-1 [UniParc]FASTA Add to basket

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        10         20         30         40         50
MNIKGKALLA GCIALAFSNM ALAEDIKVAV VGAMSGPVAQ YGDQEFTGAE 
        60         70         80         90        100
QAVADINAKG GIKGNKLQIV KYDDACDPKQ AVAVANKVVN DGIKYVIGHL 
       110        120        130        140        150
CSSSTQPASD IYEDEGILMI TPAATAPELT ARGYQLILRT TGLDSDQGPT 
       160        170        180        190        200
AAKYILEKVK PQRIAIVHDK QQYGEGLARA VQDGLKKGNA NVVFFDGITA 
       210        220        230        240        250
GEKDFSTLVA RLKKENIDFV YYGGYHPEMG QILRQARAAG LKTQFMGPEG 
       260        270        280        290        300
VANVSLSNIA GESAEGLLVT KPKNYDQVPA NKPIVDAIKA KKQDPSGAFV 
       310        320        330        340        350
WTTYAALQSL QAGLNQSDDP AEIAKYLKAN SVDTVMGPLT WDEKGDLKGF 
       360 
EFGVFDWHAN GTATDAK

Length:367

Mass (Da):39,076

Last modified:December 6, 2005 - v1

Checksum:ⁱ63F0729C251F18FD

Sequence cautionⁱ

The sequence AAB18435 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	3	I → T (PubMed:3891753).Curated	1
Sequence conflictⁱ	3	I → T (PubMed:4077929).Curated	1
Sequence conflictⁱ	3	I → T (PubMed:2195019).Curated	1
Sequence conflictⁱ	12	C → L (PubMed:3891753).Curated	1
Sequence conflictⁱ	12	C → L (PubMed:4077929).Curated	1
Sequence conflictⁱ	12	C → L (PubMed:2195019).Curated	1
Sequence conflictⁱ	16	A → G (PubMed:3891753).Curated	1
Sequence conflictⁱ	70	V → A (PubMed:3891753).Curated	1
Sequence conflictⁱ	70	V → A (PubMed:4077929).Curated	1
Sequence conflictⁱ	70	V → A (PubMed:2195019).Curated	1
Sequence conflictⁱ	70	V → A (PubMed:328304).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J05516 Genomic DNA Translation: AAA83881.1 M29377 Genomic DNA Translation: AAA24075.1 U00039 Genomic DNA Translation: AAB18435.1 Different initiation. U00096 Genomic DNA Translation: AAT48185.1 AP009048 Genomic DNA Translation: BAE77833.1
PIRⁱ	G65142 BLEC
NCBI Reference Sequences More...RefSeqⁱ	WP_001021996.1, NZ_LN832404.1 YP_026223.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaⁱ	AAT48185; AAT48185; b3460 BAE77833; BAE77833; BAE77833
GeneIDⁱ	947971
KEGGⁱ	ecj:JW3425 eco:b3460
PATRICⁱ	fig\|511145.12.peg.3559

Protein	Similar proteins		Species	Score	Length	Source
P0AD96	Leu/Ile/Val-binding protein		ECO57		367	UniRef100_P0AD98
Leu/Ile/Val-binding protein		ECOL6		367
Periplasmic binding protein of high-affinity amino acid transport system		ECOLX		386
High-affinity amino acid transport protein, periplasmic binding protein		ECOCB		386
High-affinity amino acid transport protein, periplasmic binding protein		SHIF2		386
+85

Protein	Similar proteins		Species	Score	Length	Source
P0AD96	Leu/Ile/Val-binding protein		ECO57		367	UniRef90_P0AD98
Leu/Ile/Val-binding protein		ECOL6		367
Leu/Ile/Val/Thr-binding protein		SALTY		365
Periplasmic binding protein of high-affinity amino acid transport system		ECOLX		386
High-affinity amino acid transport protein, periplasmic binding protein		ECOCB		386
+1361

Protein	Similar proteins		Species	Score	Length	Source
P0AD96	Leucine-specific-binding protein	)	ECOLI		369	UniRef50_P04816
Leucine-specific-binding protein		SALTI		369
Leucine-specific-binding protein		SALTY		369
Leu/Ile/Val-binding protein		ECO57		367
Leu/Ile/Val-binding protein		ECOL6		367
+3405

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J05516 Genomic DNA Translation: AAA83881.1 M29377 Genomic DNA Translation: AAA24075.1 U00039 Genomic DNA Translation: AAB18435.1 Different initiation. U00096 Genomic DNA Translation: AAT48185.1 AP009048 Genomic DNA Translation: BAE77833.1
PIRⁱ	G65142 BLEC
RefSeqⁱ	WP_001021996.1, NZ_LN832404.1 YP_026223.1, NC_000913.3

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1Z15	X-ray	1.70	A	24-367	[»]
	1Z16	X-ray	1.72	A	24-367	[»]
	1Z17	X-ray	1.96	A	24-367	[»]
	1Z18	X-ray	2.10	A	24-367	[»]
	2LIV	X-ray	2.40	A	24-367	[»]
ProteinModelPortalⁱ	P0AD96
SMRⁱ	P0AD96
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4259328, 24 interactors
IntActⁱ	P0AD96, 4 interactors
STRINGⁱ	316385.ECDH10B_3634

Protein family/group databases

TCDBⁱ	3.A.1.4.1 the atp-binding cassette (abc) superfamily

TCDBⁱ

3.A.1.4.1 the atp-binding cassette (abc) superfamily

2D gel databases

SWISS-2DPAGEⁱ	P0AD96

SWISS-2DPAGEⁱ

P0AD96

Proteomic databases

EPDⁱ	P0AD96
PaxDbⁱ	P0AD96
PRIDEⁱ	P0AD96

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAT48185; AAT48185; b3460 BAE77833; BAE77833; BAE77833
GeneIDⁱ	947971
KEGGⁱ	ecj:JW3425 eco:b3460
PATRICⁱ	fig\|511145.12.peg.3559

Organism-specific databases

EchoBASEⁱ	EB0534
EcoGeneⁱ	EG10539 livJ

Phylogenomic databases

eggNOGⁱ	ENOG4105CP9 Bacteria COG0683 LUCA
HOGENOMⁱ	HOG000130989
InParanoidⁱ	P0AD96
KOⁱ	K01999
PhylomeDBⁱ	P0AD96

Enzyme and pathway databases

BioCycⁱ	EcoCyc:LIVJ-MONOMER MetaCyc:LIVJ-MONOMER

BioCycⁱ

EcoCyc:LIVJ-MONOMER
MetaCyc:LIVJ-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	P0AD96
Protein Ontology More...PROⁱ	PR:P0AD96

Family and domain databases

InterProⁱ	View protein in InterPro IPR028081 Leu-bd IPR000709 Leu_Ile_Val-bd IPR028082 Peripla_BP_I
Pfamⁱ	View protein in Pfam PF13458 Peripla_BP_6, 1 hit
PRINTSⁱ	PR00337 LEUILEVALBP
SUPFAMⁱ	SSF53822 SSF53822, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	LIVJ_ECOLI
Accessionⁱ	P0AD96Primary (citable) accession number: P0AD96 Secondary accession number(s): P02917 , P76698, Q2M7C3, Q8CVL7, Q8X6S2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	December 6, 2005
	Last modified:	November 7, 2018
	This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0AD96 (LIVJ_ECOLI)

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Leu/Ile/Val-binding protein

livJ

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

2D gel databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ