UniProtKB - P0AD65 (MRDA_ECOLI)
Protein
Peptidoglycan D,D-transpeptidase MrdA
Gene
mrdA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes cross-linking of the peptidoglycan cell wall (PubMed:3009484). Responsible for the determination of the rod shape of the cell (PubMed:1103132). Is probably required for lateral peptidoglycan synthesis and maintenance of the correct diameter during lateral and centripetal growth (PubMed:12519203).3 Publications
Catalytic activityi
- Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.UniRule annotation1 Publication EC:3.4.16.4
Activity regulationi
Inhibited by mecillinam and benzylpenicillin.2 Publications
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 330 | Acyl-ester intermediateUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- penicillin binding Source: EcoCyc
- peptidoglycan L,D-transpeptidase activity Source: EcoliWiki
- serine-type D-Ala-D-Ala carboxypeptidase activity Source: EcoCyc
GO - Biological processi
- cell wall organization Source: EcoCyc
- peptidoglycan biosynthetic process Source: EcoCyc
- regulation of cell shape Source: EcoliWiki
- response to antibiotic Source: EcoliWiki
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Protease |
Biological process | Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10606-MONOMER MetaCyc:EG10606-MONOMER |
UniPathwayi | UPA00219 |
Names & Taxonomyi
Protein namesi | Recommended name: Peptidoglycan D,D-transpeptidase MrdAUniRule annotationCurated (EC:3.4.16.4UniRule annotation1 Publication)Alternative name(s): Penicillin-binding protein 22 PublicationsUniRule annotation Short name: PBP-21 PublicationUniRule annotation |
Gene namesi | Name:mrdA1 PublicationUniRule annotation Synonyms:pbpA2 Publications Ordered Locus Names:b0635, JW0630 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane UniRule annotation2 Publications; Single-pass membrane protein UniRule annotation Note: Localizes preferentially in the lateral wall and at mid-cell in comparison with the old cell pole. Localization at mid-cell is dependent on active FtsI.1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 22 – 42 | HelicalUniRule annotationAdd BLAST | 21 |
GO - Cellular componenti
- integral component of plasma membrane Source: EcoliWiki
- outer membrane-bounded periplasmic space Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Temperature-sensitive mutants grow as normal rods at 30 degrees Celsius but grow and divide as cocci during prolonged culturing at 42 degrees Celsius.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 330 | S → C: No longer binds penicillin. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL1840 CHEMBL2354313 |
DrugBanki | DB01163, Amdinocillin DB01602, Bacampicillin DB00578, Carbenicillin DB09319, Carindacillin DB01327, Cefazolin DB01413, Cefepime DB01328, Cefonicid DB01329, Cefoperazone DB00438, Ceftazidime DB01415, Ceftibuten DB09050, Ceftolozane DB01000, Cyclacillin DB06211, Doripenem DB00303, Ertapenem DB01598, Imipenem DB00948, Mezlocillin |
DrugCentrali | P0AD65 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000195444 | 1 – 633 | Peptidoglycan D,D-transpeptidase MrdAAdd BLAST | 633 |
Proteomic databases
jPOSTi | P0AD65 |
PaxDbi | P0AD65 |
PRIDEi | P0AD65 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259912, 573 interactors |
DIPi | DIP-48190N |
IntActi | P0AD65, 2 interactors |
STRINGi | 511145.b0635 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AD65 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0768, Bacteria |
HOGENOMi | CLU_009289_1_2_6 |
InParanoidi | P0AD65 |
PhylomeDBi | P0AD65 |
Family and domain databases
Gene3Di | 3.40.710.10, 1 hit |
HAMAPi | MF_02081, MrdA_transpept, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR005311, PBP_dimer IPR036138, PBP_dimer_sf IPR001460, PCN-bd_Tpept IPR017790, Penicillin-binding_protein_2 |
Pfami | View protein in Pfam PF03717, PBP_dimer, 1 hit PF00905, Transpeptidase, 1 hit |
SUPFAMi | SSF56519, SSF56519, 1 hit SSF56601, SSF56601, 1 hit |
TIGRFAMsi | TIGR03423, pbp2_mrdA, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AD65-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLQNSFRDY TAESALFVRR ALVAFLGILL LTGVLIANLY NLQIVRFTDY
60 70 80 90 100
QTRSNENRIK LVPIAPSRGI IYDRNGIPLA LNRTIYQIEM MPEKVDNVQQ
110 120 130 140 150
TLDALRSVVD LTDDDIAAFR KERARSHRFT SIPVKTNLTE VQVARFAVNQ
160 170 180 190 200
YRFPGVEVKG YKRRYYPYGS ALTHVIGYVS KINDKDVERL NNDGKLANYA
210 220 230 240 250
ATHDIGKLGI ERYYEDVLHG QTGYEEVEVN NRGRVIRQLK EVPPQAGHDI
260 270 280 290 300
YLTLDLKLQQ YIETLLAGSR AAVVVTDPRT GGVLALVSTP SYDPNLFVDG
310 320 330 340 350
ISSKDYSALL NDPNTPLVNR ATQGVYPPAS TVKPYVAVSA LSAGVITRNT
360 370 380 390 400
TLFDPGWWQL PGSEKRYRDW KKWGHGRLNV TRSLEESADT FFYQVAYDMG
410 420 430 440 450
IDRLSEWMGK FGYGHYTGID LAEERSGNMP TREWKQKRFK KPWYQGDTIP
460 470 480 490 500
VGIGQGYWTA TPIQMSKALM ILINDGIVKV PHLLMSTAED GKQVPWVQPH
510 520 530 540 550
EPPVGDIHSG YWELAKDGMY GVANRPNGTA HKYFASAPYK IAAKSGTAQV
560 570 580 590 600
FGLKANETYN AHKIAERLRD HKLMTAFAPY NNPQVAVAMI LENGGAGPAV
610 620 630
GTLMRQILDH IMLGDNNTDL PAENPAVAAA EDH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04516 Genomic DNA Translation: CAA28201.1 U82598 Genomic DNA Translation: AAB40835.1 U00096 Genomic DNA Translation: AAC73736.1 AP009048 Genomic DNA Translation: BAA35282.1 M22857 Genomic DNA Translation: AAA24570.1 |
PIRi | C24995, ZPECP2 |
RefSeqi | NP_415168.1, NC_000913.3 WP_000776191.1, NZ_STEB01000031.1 |
Genome annotation databases
EnsemblBacteriai | AAC73736; AAC73736; b0635 BAA35282; BAA35282; BAA35282 |
GeneIDi | 49585602 945240 |
KEGGi | ecj:JW0630 eco:b0635 |
PATRICi | fig|1411691.4.peg.1633 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04516 Genomic DNA Translation: CAA28201.1 U82598 Genomic DNA Translation: AAB40835.1 U00096 Genomic DNA Translation: AAC73736.1 AP009048 Genomic DNA Translation: BAA35282.1 M22857 Genomic DNA Translation: AAA24570.1 |
PIRi | C24995, ZPECP2 |
RefSeqi | NP_415168.1, NC_000913.3 WP_000776191.1, NZ_STEB01000031.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6G9F | X-ray | 2.35 | A | 57-615 | [»] | |
6G9P | X-ray | 2.10 | A | 52-633 | [»] | |
6G9S | X-ray | 2.00 | A | 52-633 | [»] | |
SMRi | P0AD65 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259912, 573 interactors |
DIPi | DIP-48190N |
IntActi | P0AD65, 2 interactors |
STRINGi | 511145.b0635 |
Chemistry databases
ChEMBLi | CHEMBL1840 CHEMBL2354313 |
DrugBanki | DB01163, Amdinocillin DB01602, Bacampicillin DB00578, Carbenicillin DB09319, Carindacillin DB01327, Cefazolin DB01413, Cefepime DB01328, Cefonicid DB01329, Cefoperazone DB00438, Ceftazidime DB01415, Ceftibuten DB09050, Ceftolozane DB01000, Cyclacillin DB06211, Doripenem DB00303, Ertapenem DB01598, Imipenem DB00948, Mezlocillin |
DrugCentrali | P0AD65 |
Proteomic databases
jPOSTi | P0AD65 |
PaxDbi | P0AD65 |
PRIDEi | P0AD65 |
Genome annotation databases
EnsemblBacteriai | AAC73736; AAC73736; b0635 BAA35282; BAA35282; BAA35282 |
GeneIDi | 49585602 945240 |
KEGGi | ecj:JW0630 eco:b0635 |
PATRICi | fig|1411691.4.peg.1633 |
Organism-specific databases
EchoBASEi | EB0601 |
Phylogenomic databases
eggNOGi | COG0768, Bacteria |
HOGENOMi | CLU_009289_1_2_6 |
InParanoidi | P0AD65 |
PhylomeDBi | P0AD65 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | EcoCyc:EG10606-MONOMER MetaCyc:EG10606-MONOMER |
Miscellaneous databases
PROi | PR:P0AD65 |
Family and domain databases
Gene3Di | 3.40.710.10, 1 hit |
HAMAPi | MF_02081, MrdA_transpept, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR005311, PBP_dimer IPR036138, PBP_dimer_sf IPR001460, PCN-bd_Tpept IPR017790, Penicillin-binding_protein_2 |
Pfami | View protein in Pfam PF03717, PBP_dimer, 1 hit PF00905, Transpeptidase, 1 hit |
SUPFAMi | SSF56519, SSF56519, 1 hit SSF56601, SSF56601, 1 hit |
TIGRFAMsi | TIGR03423, pbp2_mrdA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MRDA_ECOLI | |
Accessioni | P0AD65Primary (citable) accession number: P0AD65 Secondary accession number(s): P08150 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | December 2, 2020 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families