mrdA

Functionⁱ

Catalyzes cross-linking of the peptidoglycan cell wall (PubMed:3009484). Responsible for the determination of the rod shape of the cell (PubMed:1103132). Is probably required for lateral peptidoglycan synthesis and maintenance of the correct diameter during lateral and centripetal growth (PubMed:12519203).3 Publications

Catalytic activityⁱ

Preferential cleavage: (Ac)₂-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.UniRule annotation1 Publication

Activity regulationⁱ

Inhibited by mecillinam and benzylpenicillin.2 Publications

Pathwayⁱ: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	330	Acyl-ester intermediateUniRule annotation1 Publication		1

GO - Molecular functionⁱ

drug binding
Source: EcoCyc
Inferred from direct assayⁱ
penicillin binding
Source: EcoliWiki
Inferred from mutant phenotypeⁱ
- 1103132
peptidoglycan L,D-transpeptidase activity
Source: EcoliWiki
Inferred from direct assayⁱ
- 6297485
serine-type D-Ala-D-Ala carboxypeptidase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 3009484

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell wall organization
Source: EcoCyc
Inferred from mutant phenotypeⁱ
peptidoglycan biosynthetic process
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 18390656
- 8760906
regulation of cell shape
Source: EcoliWiki
Inferred from mutant phenotypeⁱ
- 1103132
response to antibiotic
Source: EcoliWiki
Inferred from mutant phenotypeⁱ
- 1103132
response to drug
Source: EcoCyc
Inferred from direct assayⁱ

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Carboxypeptidase, Hydrolase, Protease
Biological process	Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG10606-MONOMER MetaCyc:EG10606-MONOMER
UniPathwayⁱ	UPA00219

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Peptidoglycan D,D-transpeptidase MrdAUniRule annotationCurated (EC:3.4.16.4UniRule annotation1 Publication) Alternative name(s): Penicillin-binding protein 22 PublicationsUniRule annotation Short name: PBP-21 PublicationUniRule annotation
Gene namesⁱ	Name:mrdA1 PublicationUniRule annotation Synonyms:pbpA2 Publications Ordered Locus Names:b0635, JW0630
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10606 mrdA

EcoGeneⁱ

EG10606 mrdA

Subcellular locationⁱ

Cell inner membrane
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_02081
2 Publications
Manual assertion based on experiment inⁱ
- Ref.8
  "Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12."
  Spratt B.G.
  Proc. Natl. Acad. Sci. U.S.A. 72:2999-3003(1975) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBCELLULAR LOCATION, ACTIVITY REGULATION.
- Ref.10
  "Peptidoglycan synthetic activities in membranes of Escherichia coli caused by overproduction of penicillin-binding protein 2 and rodA protein."
  Ishino F., Park W., Tomioka S., Tamaki S., Takase I., Kunugita K., Matsuzawa H., Asoh S., Ohta T., Spratt B.G.
  J. Biol. Chem. 261:7024-7031(1986) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, PATHWAY, SUBCELLULAR LOCATION.
; Single-pass membrane protein
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_02081

Note:

Localizes preferentially in the lateral wall and at mid-cell in comparison with the old cell pole. Localization at mid-cell is dependent on active FtsI.1 Publication

Topology

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transmembraneⁱ	22 – 42	HelicalUniRule annotationAdd BLAST		21

GO - Cellular componentⁱ

integral component of plasma membrane
Source: EcoliWiki
Inferred from direct assayⁱ
- 1103132

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Temperature-sensitive mutants grow as normal rods at 30 degrees Celsius but grow and divide as cocci during prolonged culturing at 42 degrees Celsius.1 Publication

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	330	S → C: No longer binds penicillin. 1 Publication		1

Chemistry databases

ChEMBLⁱ	CHEMBL1840
Drug and drug target database More...DrugBankⁱ	DB01163 Amdinocillin DB01327 Cefazolin DB01413 Cefepime DB01328 Cefonicid DB01329 Cefoperazone DB00438 Ceftazidime DB01415 Ceftibuten DB06211 Doripenem DB00303 Ertapenem DB01598 Imipenem DB00948 Mezlocillin

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000195444	1 – 633	Peptidoglycan D,D-transpeptidase MrdAAdd BLAST		633

Proteomic databases

PaxDbⁱ	P0AD65
PRIDEⁱ	P0AD65

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4259912, 573 interactors
Database of interacting proteins More...DIPⁱ	DIP-48190N
IntActⁱ	P0AD65, 2 interactors
STRINGⁱ	316385.ECDH10B_0704

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P0AD65
SMRⁱ	P0AD65
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the transpeptidase family. MrdA subfamily.UniRule annotationCurated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG4105CJN Bacteria COG0768 LUCA
HOGENOMⁱ	HOG000266120
InParanoidⁱ	P0AD65
KEGG Orthology (KO) More...KOⁱ	K05515
OMAⁱ	SCDTYYY
PhylomeDBⁱ	P0AD65

Family and domain databases

HAMAPⁱ	MF_02081 MrdA_transpept, 1 hit
InterProⁱ	View protein in InterPro IPR012338 Beta-lactam/transpept-like IPR005311 PBP_dimer IPR036138 PBP_dimer_sf IPR001460 PCN-bd_Tpept IPR017790 Penicillin-binding_protein_2
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03717 PBP_dimer, 1 hit PF00905 Transpeptidase, 1 hit
SUPFAMⁱ	SSF56519 SSF56519, 1 hit SSF56601 SSF56601, 1 hit
TIGRFAMsⁱ	TIGR03423 pbp2_mrdA, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0AD65-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKLQNSFRDY TAESALFVRR ALVAFLGILL LTGVLIANLY NLQIVRFTDY 
        60         70         80         90        100
QTRSNENRIK LVPIAPSRGI IYDRNGIPLA LNRTIYQIEM MPEKVDNVQQ 
       110        120        130        140        150
TLDALRSVVD LTDDDIAAFR KERARSHRFT SIPVKTNLTE VQVARFAVNQ 
       160        170        180        190        200
YRFPGVEVKG YKRRYYPYGS ALTHVIGYVS KINDKDVERL NNDGKLANYA 
       210        220        230        240        250
ATHDIGKLGI ERYYEDVLHG QTGYEEVEVN NRGRVIRQLK EVPPQAGHDI 
       260        270        280        290        300
YLTLDLKLQQ YIETLLAGSR AAVVVTDPRT GGVLALVSTP SYDPNLFVDG 
       310        320        330        340        350
ISSKDYSALL NDPNTPLVNR ATQGVYPPAS TVKPYVAVSA LSAGVITRNT 
       360        370        380        390        400
TLFDPGWWQL PGSEKRYRDW KKWGHGRLNV TRSLEESADT FFYQVAYDMG 
       410        420        430        440        450
IDRLSEWMGK FGYGHYTGID LAEERSGNMP TREWKQKRFK KPWYQGDTIP 
       460        470        480        490        500
VGIGQGYWTA TPIQMSKALM ILINDGIVKV PHLLMSTAED GKQVPWVQPH 
       510        520        530        540        550
EPPVGDIHSG YWELAKDGMY GVANRPNGTA HKYFASAPYK IAAKSGTAQV 
       560        570        580        590        600
FGLKANETYN AHKIAERLRD HKLMTAFAPY NNPQVAVAMI LENGGAGPAV 
       610        620        630 
GTLMRQILDH IMLGDNNTDL PAENPAVAAA EDH

Length:633

Mass (Da):70,857

Last modified:August 1, 1988 - v1

Checksum:ⁱFE2305C002743AF6

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X04516 Genomic DNA Translation: CAA28201.1 U82598 Genomic DNA Translation: AAB40835.1 U00096 Genomic DNA Translation: AAC73736.1 AP009048 Genomic DNA Translation: BAA35282.1 M22857 Genomic DNA Translation: AAA24570.1
PIRⁱ	C24995 ZPECP2
NCBI Reference Sequences More...RefSeqⁱ	NP_415168.1, NC_000913.3 WP_000776191.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73736; AAC73736; b0635 BAA35282; BAA35282; BAA35282
GeneIDⁱ	945240
KEGGⁱ	ecj:JW0630 eco:b0635
PATRICⁱ	fig\|1411691.4.peg.1633

Protein	Similar proteins		Species	Score	Length	Source
P0AD65	Peptidoglycan D,D-transpeptidase MrdA		ECO57		633	UniRef100_P0AD67
Peptidoglycan D,D-transpeptidase MrdA		ECOL6		633
Peptidoglycan D,D-transpeptidase MrdA		Escherichia coli TW10509		636
Peptidoglycan D,D-transpeptidase MrdA		Escherichia coli NCCP15648		633
Penicillin-binding protein 2		Escherichia coli O145 str. RM9872		633
+212

Protein	Similar proteins		Species	Score	Length	Source
P0AD65	Peptidoglycan D,D-transpeptidase MrdA		ECO57		633	UniRef90_P0AD67
Peptidoglycan D,D-transpeptidase MrdA		ECOL6		633
Peptidoglycan D,D-transpeptidase MrdA		Escherichia coli TW10509		636
Peptidoglycan D,D-transpeptidase MrdA		Escherichia coli NCCP15648		633
Penicillin-binding protein 2		Escherichia coli O145 str. RM9872		633
+1119

Protein	Similar proteins		Species	Score	Length	Source
P0AD65	Peptidoglycan D,D-transpeptidase MrdA		ECO57		633	UniRef50_P0AD67
Peptidoglycan D,D-transpeptidase MrdA		ECOL6		633
Peptidoglycan D,D-transpeptidase MrdA		Escherichia coli TW10509		636
Peptidoglycan D,D-transpeptidase MrdA		Escherichia coli NCCP15648		633
Penicillin-binding protein 2		Escherichia coli O145 str. RM9872		633
+1926

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X04516 Genomic DNA Translation: CAA28201.1 U82598 Genomic DNA Translation: AAB40835.1 U00096 Genomic DNA Translation: AAC73736.1 AP009048 Genomic DNA Translation: BAA35282.1 M22857 Genomic DNA Translation: AAA24570.1
PIRⁱ	C24995 ZPECP2
RefSeqⁱ	NP_415168.1, NC_000913.3 WP_000776191.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P0AD65
SMRⁱ	P0AD65
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4259912, 573 interactors
DIPⁱ	DIP-48190N
IntActⁱ	P0AD65, 2 interactors
STRINGⁱ	316385.ECDH10B_0704

Chemistry databases

ChEMBLⁱ	CHEMBL1840
DrugBankⁱ	DB01163 Amdinocillin DB01327 Cefazolin DB01413 Cefepime DB01328 Cefonicid DB01329 Cefoperazone DB00438 Ceftazidime DB01415 Ceftibuten DB06211 Doripenem DB00303 Ertapenem DB01598 Imipenem DB00948 Mezlocillin

Proteomic databases

PaxDbⁱ	P0AD65
PRIDEⁱ	P0AD65

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73736; AAC73736; b0635 BAA35282; BAA35282; BAA35282
GeneIDⁱ	945240
KEGGⁱ	ecj:JW0630 eco:b0635
PATRICⁱ	fig\|1411691.4.peg.1633

Organism-specific databases

EchoBASEⁱ	EB0601
EcoGeneⁱ	EG10606 mrdA

Phylogenomic databases

eggNOGⁱ	ENOG4105CJN Bacteria COG0768 LUCA
HOGENOMⁱ	HOG000266120
InParanoidⁱ	P0AD65
KOⁱ	K05515
OMAⁱ	SCDTYYY
PhylomeDBⁱ	P0AD65

Enzyme and pathway databases

UniPathwayⁱ	UPA00219
BioCycⁱ	EcoCyc:EG10606-MONOMER MetaCyc:EG10606-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P0AD65

PROⁱ

PR:P0AD65

Family and domain databases

HAMAPⁱ	MF_02081 MrdA_transpept, 1 hit
InterProⁱ	View protein in InterPro IPR012338 Beta-lactam/transpept-like IPR005311 PBP_dimer IPR036138 PBP_dimer_sf IPR001460 PCN-bd_Tpept IPR017790 Penicillin-binding_protein_2
Pfamⁱ	View protein in Pfam PF03717 PBP_dimer, 1 hit PF00905 Transpeptidase, 1 hit
SUPFAMⁱ	SSF56519 SSF56519, 1 hit SSF56601 SSF56601, 1 hit
TIGRFAMsⁱ	TIGR03423 pbp2_mrdA, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MRDA_ECOLI
Accessionⁱ	P0AD65Primary (citable) accession number: P0AD65 Secondary accession number(s): P08150
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
	Last sequence update:	August 1, 1988
	Last modified:	September 12, 2018
	This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0AD65 (MRDA_ECOLI)

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Peptidoglycan D,D-transpeptidase MrdA

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: peptidoglycan biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

Pathwayⁱ: peptidoglycan biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ