UniProtKB - P0AD57 (ISPB_ECOLI)
Protein
Octaprenyl diphosphate synthase
Gene
ispB
Organism
Escherichia coli (strain K12)
Status
Functioni
Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone.1 Publication
Catalytic activityi
- (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate = all-trans-octaprenyl diphosphate + 5 diphosphate1 PublicationEC:2.5.1.901 Publication
Cofactori
Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 45 | Isopentenyl diphosphateBy similarity | 1 | |
Binding sitei | 48 | Isopentenyl diphosphateBy similarity | 1 | |
Binding sitei | 77 | Isopentenyl diphosphateBy similarity | 1 | |
Metal bindingi | 84 | Magnesium 1By similarity | 1 | |
Metal bindingi | 84 | Magnesium 2By similarity | 1 | |
Metal bindingi | 88 | Magnesium 1By similarity | 1 | |
Metal bindingi | 88 | Magnesium 2By similarity | 1 | |
Binding sitei | 93 | Polyprenyl diphosphateBy similarity | 1 | |
Binding sitei | 94 | Isopentenyl diphosphateBy similarity | 1 | |
Binding sitei | 170 | Polyprenyl diphosphateBy similarity | 1 | |
Binding sitei | 171 | Polyprenyl diphosphateBy similarity | 1 | |
Binding sitei | 208 | Polyprenyl diphosphateBy similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
- prenyltransferase activity Source: EcoCyc
GO - Biological processi
- isoprenoid biosynthetic process Source: GO_Central
- polyprenol biosynthetic process Source: EcoCyc
- ubiquinone biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Transferase |
Biological process | Isoprene biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:OPPSYN-MONOMER MetaCyc:OPPSYN-MONOMER |
BRENDAi | 2.5.1.90, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Octaprenyl diphosphate synthase1 Publication (EC:2.5.1.901 Publication)Alternative name(s): All-trans-octaprenyl-diphosphate synthase Octaprenyl pyrophosphate synthase Short name: OPP synthase |
Gene namesi | Name:ispB Synonyms:cel, yhbD Ordered Locus Names:b3187, JW3154 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000124005 | 1 – 323 | Octaprenyl diphosphate synthaseAdd BLAST | 323 |
Proteomic databases
jPOSTi | P0AD57 |
PaxDbi | P0AD57 |
PRIDEi | P0AD57 |
Interactioni
Binary interactionsi
Hide detailsP0AD57
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4261111, 137 interactors 851687, 3 interactors |
IntActi | P0AD57, 5 interactors |
MINTi | P0AD57 |
STRINGi | 511145.b3187 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AD57 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the FPP/GGPP synthase family.Curated
Phylogenomic databases
eggNOGi | COG0142, Bacteria |
HOGENOMi | CLU_014015_2_0_6 |
InParanoidi | P0AD57 |
PhylomeDBi | P0AD57 |
Family and domain databases
CDDi | cd00685, Trans_IPPS_HT, 1 hit |
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR000092, Polyprenyl_synt IPR033749, Polyprenyl_synt_CS |
Pfami | View protein in Pfam PF00348, polyprenyl_synt, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
PROSITEi | View protein in PROSITE PS00723, POLYPRENYL_SYNTHASE_1, 1 hit PS00444, POLYPRENYL_SYNTHASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AD57-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNLEKINELT AQDMAGVNAA ILEQLNSDVQ LINQLGYYIV SGGGKRIRPM
60 70 80 90 100
IAVLAARAVG YEGNAHVTIA ALIEFIHTAT LLHDDVVDES DMRRGKATAN
110 120 130 140 150
AAFGNAASVL VGDFIYTRAF QMMTSLGSLK VLEVMSEAVN VIAEGEVLQL
160 170 180 190 200
MNVNDPDITE ENYMRVIYSK TARLFEAAAQ CSGILAGCTP EEEKGLQDYG
210 220 230 240 250
RYLGTAFQLI DDLLDYNADG EQLGKNVGDD LNEGKPTLPL LHAMHHGTPE
260 270 280 290 300
QAQMIRTAIE QGNGRHLLEP VLEAMNACGS LEWTRQRAEE EADKAIAALQ
310 320
VLPDTPWREA LIGLAHIAVQ RDR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13267 Genomic DNA No translation available. U18997 Genomic DNA Translation: AAA57988.1 U00096 Genomic DNA Translation: AAC76219.1 AP009048 Genomic DNA Translation: BAE77231.1 X68873 Genomic DNA Translation: CAA48735.1 |
PIRi | E65109 |
RefSeqi | NP_417654.1, NC_000913.3 WP_001047336.1, NZ_STEB01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC76219; AAC76219; b3187 BAE77231; BAE77231; BAE77231 |
GeneIDi | 58460038 947364 |
KEGGi | ecj:JW3154 eco:b3187 |
PATRICi | fig|1411691.4.peg.3544 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13267 Genomic DNA No translation available. U18997 Genomic DNA Translation: AAA57988.1 U00096 Genomic DNA Translation: AAC76219.1 AP009048 Genomic DNA Translation: BAE77231.1 X68873 Genomic DNA Translation: CAA48735.1 |
PIRi | E65109 |
RefSeqi | NP_417654.1, NC_000913.3 WP_001047336.1, NZ_STEB01000012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5ZE6 | X-ray | 2.50 | A/B/C/D | 1-323 | [»] | |
5ZLF | X-ray | 2.85 | A/B/C/D | 1-323 | [»] | |
SMRi | P0AD57 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261111, 137 interactors 851687, 3 interactors |
IntActi | P0AD57, 5 interactors |
MINTi | P0AD57 |
STRINGi | 511145.b3187 |
Chemistry databases
ChEMBLi | CHEMBL3309021 |
Proteomic databases
jPOSTi | P0AD57 |
PaxDbi | P0AD57 |
PRIDEi | P0AD57 |
Genome annotation databases
EnsemblBacteriai | AAC76219; AAC76219; b3187 BAE77231; BAE77231; BAE77231 |
GeneIDi | 58460038 947364 |
KEGGi | ecj:JW3154 eco:b3187 |
PATRICi | fig|1411691.4.peg.3544 |
Organism-specific databases
EchoBASEi | EB0017 |
Phylogenomic databases
eggNOGi | COG0142, Bacteria |
HOGENOMi | CLU_014015_2_0_6 |
InParanoidi | P0AD57 |
PhylomeDBi | P0AD57 |
Enzyme and pathway databases
BioCyci | EcoCyc:OPPSYN-MONOMER MetaCyc:OPPSYN-MONOMER |
BRENDAi | 2.5.1.90, 2026 |
Miscellaneous databases
PROi | PR:P0AD57 |
Family and domain databases
CDDi | cd00685, Trans_IPPS_HT, 1 hit |
Gene3Di | 1.10.600.10, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR000092, Polyprenyl_synt IPR033749, Polyprenyl_synt_CS |
Pfami | View protein in Pfam PF00348, polyprenyl_synt, 1 hit |
SUPFAMi | SSF48576, SSF48576, 1 hit |
PROSITEi | View protein in PROSITE PS00723, POLYPRENYL_SYNTHASE_1, 1 hit PS00444, POLYPRENYL_SYNTHASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ISPB_ECOLI | |
Accessioni | P0AD57Primary (citable) accession number: P0AD57 Secondary accession number(s): P19641, Q2M925 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | April 7, 2021 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families