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Protein

Lipid A biosynthesis palmitoleoyltransferase

Gene

lpxP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of palmitoleate from palmitoleoyl-acyl carrier protein (ACP) to Kdo2-lipid IV(A) to form Kdo2-(palmitoleoyl)-lipid IV(A). Required for the biosynthesis of a distinct molecular species of lipid A, which is present only in cells grown at low temperatures. It may confer a selective advantage to cells growing at lower temperatures by making the outer membrane a more effective barrier to harmful chemicals.2 Publications

Miscellaneous

About two-thirds of the lipid A residues of cells grown at 12 degrees Celsius are acylated with palmitoleate rather than laurate.1 Publication

Catalytic activityi

A (9Z)-hexadecenoyl-[acyl-carrier protein] + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) = (Kdo)2-((9Z)-hexadecenoyl)-lipid IV(A) + a holo-[acyl-carrier protein].UniRule annotation1 Publication

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

  • Kdo2-lipid A biosynthetic process Source: EcoCyc
  • lipid A biosynthetic process Source: InterPro
  • lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
  • response to cold Source: EcoCyc

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipopolysaccharide biosynthesis, Stress response

Enzyme and pathway databases

BioCyciEcoCyc:PALMITOTRANS-MONOMER
MetaCyc:PALMITOTRANS-MONOMER
BRENDAi2.3.1.B30 2026
UniPathwayi
UPA00030

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid A biosynthesis palmitoleoyltransferase1 PublicationUniRule annotation (EC:2.3.1.242UniRule annotation1 Publication)
Alternative name(s):
Kdo(2)-lipid IV(A) palmitoleoyltransferaseUniRule annotation
Gene namesi
Name:lpxP1 PublicationUniRule annotation
Synonyms:ddg
Ordered Locus Names:b2378, JW2375
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12901 lpxP

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei20 – 40HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption does not confer any obvious growth phenotype in nutrient broth at low temperatures, but mutant shows a 10-fold increase in sensitivity to rifampicin and vancomycin at 12 degrees Celsius compared with wild type cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002017811 – 306Lipid A biosynthesis palmitoleoyltransferaseAdd BLAST306

Proteomic databases

PaxDbiP0ACV2
PRIDEiP0ACV2

Expressioni

Inductioni

Induced more than 30-fold upon cold shock. The induced activity is maximal after 2 h of cold shock, and then gradually declines but does not disappear (PubMed:10092655). Induced in persister cells (PubMed:16768798).2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4260930, 144 interactors
IntActiP0ACV2, 6 interactors
STRINGi316385.ECDH10B_2543

Structurei

3D structure databases

ProteinModelPortaliP0ACV2
SMRiP0ACV2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi132 – 137HXXXXD motifUniRule annotation6

Sequence similaritiesi

Belongs to the LpxL/LpxM/LpxP family. LpxP subfamily.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D1S Bacteria
COG1560 LUCA
HOGENOMiHOG000265960
InParanoidiP0ACV2
KOiK12974
OMAiFWFHRRF
PhylomeDBiP0ACV2

Family and domain databases

CDDicd07984 LPLAT_LABLAT-like, 1 hit
HAMAPiMF_01942 Lipid_A_LpxL_LpxP, 1 hit
MF_01943 Lipid_A_LpxP, 1 hit
InterProiView protein in InterPro
IPR004960 LipA_acyltrans
IPR011920 Lipid_A_LpxL_LpxP
IPR030857 Lipid_A_LpxP
PANTHERiPTHR30606 PTHR30606, 1 hit
PfamiView protein in Pfam
PF03279 Lip_A_acyltrans, 1 hit
PIRSFiPIRSF026649 MsbB, 1 hit
TIGRFAMsiTIGR02207 lipid_A_htrB, 1 hit

Sequencei

Sequence statusi: Complete.

P0ACV2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFPQCKFSRE FLHPRYWLTW FGLGVLWLWV QLPYPVLCFL GTRIGAMARP
60 70 80 90 100
FLKRRESIAR KNLELCFPQH SAEEREKMIA ENFRSLGMAL VETGMAWFWP
110 120 130 140 150
DSRVRKWFDV EGLDNLKRAQ MQNRGVMVVG VHFMSLELGG RVMGLCQPMM
160 170 180 190 200
ATYRPHNNQL MEWVQTRGRM RSNKAMIGRN NLRGIVGALK KGEAVWFAPD
210 220 230 240 250
QDYGRKGSSF APFFAVENVA TTNGTYVLSR LSGAAMLTVT MVRKADYSGY
260 270 280 290 300
RLFITPEMEG YPTDENQAAA YMNKIIEKEI MRAPEQYLWI HRRFKTRPVG

ESSLYI
Length:306
Mass (Da):35,493
Last modified:November 22, 2005 - v1
Checksum:i58327F3D96C9E7B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49787 Genomic DNA Translation: AAB66658.1
U00096 Genomic DNA Translation: AAC75437.2
AP009048 Genomic DNA Translation: BAA16248.1
RefSeqiNP_416879.4, NC_000913.3
WP_000484404.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75437; AAC75437; b2378
BAA16248; BAA16248; BAA16248
GeneIDi946847
KEGGiecj:JW2375
eco:b2378
PATRICifig|1411691.4.peg.4351

Similar proteinsi

Entry informationi

Entry nameiLPXP_ECOLI
AccessioniPrimary (citable) accession number: P0ACV2
Secondary accession number(s): P76522, P76949
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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