UniProtKB - P0ACV0 (LPXL_ECOLI)
Protein
Lipid A biosynthesis lauroyltransferase
Gene
lpxL
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the transfer of laurate from lauroyl-acyl carrier protein (ACP) to Kdo2-lipid IV(A) to form Kdo2-(lauroyl)-lipid IV(A). Has 10 fold selectivity for lauroyl-ACP over myristoyl-ACP. In vitro, can also catalyze a slow second acylation reaction leading to the formation of Kdo2-(dilauroyl)-lipid IV(A).3 Publications
Catalytic activityi
- α-Kdo-(2→4)-α-Kdo-(2→6)-lipid IVA + dodecanoyl-[ACP] = dodecanoyl-(Kdo)2-lipid IVA + holo-[ACP]UniRule annotation1 PublicationEC:2.3.1.241UniRule annotation1 Publication
Kineticsi
- KM=7 µM for lauroyl-ACP1 Publication
- KM=15 µM for Kdo2-lipid IV(A)1 Publication
- Vmax=95 µmol/min/mg enzyme toward lauroyl-ACP1 Publication
- Vmax=221 µmol/min/mg enzyme toward Kdo2-lipid IV(A)1 Publication
: KDO(2)-lipid A biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A).UniRule annotation3 PublicationsProteins known to be involved in the 4 steps of the subpathway in this organism are:
- 3-deoxy-D-manno-octulosonic acid transferase (waaA)
- 3-deoxy-D-manno-octulosonic acid transferase (waaA)
- Lipid A biosynthesis lauroyltransferase (lpxL), Lipid A biosynthesis lauroyltransferase (lpxL)
- Lipid A biosynthesis myristoyltransferase (lpxM), Lipid A biosynthesis myristoyltransferase (msbB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A), the pathway KDO(2)-lipid A biosynthesis and in Glycolipid biosynthesis.
Pathwayi: lipopolysaccharide biosynthesis
This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation3 PublicationsView all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.
GO - Molecular functioni
- lauroyltransferase activity Source: UniProtKB-UniRule
- transferase activity Source: EcoCyc
GO - Biological processi
- Kdo2-lipid A biosynthetic process Source: UniProtKB-UniRule
- lipid A biosynthetic process Source: EcoliWiki
- lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Lipopolysaccharide biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:LAUROYLACYLTRAN-MONOMER MetaCyc:LAUROYLACYLTRAN-MONOMER |
BRENDAi | 2.3.1.B29, 2026 |
UniPathwayi | UPA00030 UPA00360;UER00485 |
Names & Taxonomyi
Protein namesi | Recommended name: Lipid A biosynthesis lauroyltransferase1 PublicationUniRule annotation (EC:2.3.1.241UniRule annotation1 Publication)Alternative name(s): Kdo(2)-lipid IV(A) lauroyltransferaseUniRule annotation |
Gene namesi | Name:lpxL1 PublicationUniRule annotation Synonyms:htrB1 Publication, waaM Ordered Locus Names:b1054Imported, JW1041Imported |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane UniRule annotation3 Publications; Single-pass membrane protein UniRule annotation
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 17 – 37 | HelicalUniRule annotationAdd BLAST | 21 |
GO - Cellular componenti
- integral component of plasma membrane Source: UniProtKB-UniRule
- membrane Source: EcoliWiki
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Inactivation leads to bacterial death at temperatures above 33 degrees Celsius. Phenotype at nonpermissive temperatures includes an arrest of cell division followed by the formation of bulges or filaments.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 132 | H → A: Almost loss of activity. 1 Publication | 1 | |
Mutagenesisi | 137 | E → A: Almost loss of activity. 1 Publication | 1 | |
Mutagenesisi | 169 | R → A: 169-fold decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 200 | D → A: 14-fold decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 238 | P → A: Slight decrease in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201774 | 1 – 306 | Lipid A biosynthesis lauroyltransferaseAdd BLAST | 306 |
Proteomic databases
jPOSTi | P0ACV0 |
PaxDbi | P0ACV0 |
PRIDEi | P0ACV0 |
Expressioni
Inductioni
Is expressed at all temperatures, but accumulation of htrB transcripts slightly declines with raising temperature. Thus, its expression is not induced by heat shock.1 Publication
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262842, 71 interactors |
IntActi | P0ACV0, 9 interactors |
STRINGi | 511145.b1054 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 132 – 137 | HXXXXD motifUniRule annotation1 Publication | 6 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1560, Bacteria |
HOGENOMi | CLU_049421_1_1_6 |
InParanoidi | P0ACV0 |
PhylomeDBi | P0ACV0 |
Family and domain databases
CDDi | cd07984, LPLAT_LABLAT-like, 1 hit |
HAMAPi | MF_01942, Lipid_A_LpxL_LpxP, 1 hit |
InterProi | View protein in InterPro IPR004960, LipA_acyltrans IPR011920, Lipid_A_LpxL_LpxP |
PANTHERi | PTHR30606, PTHR30606, 1 hit |
Pfami | View protein in Pfam PF03279, Lip_A_acyltrans, 1 hit |
PIRSFi | PIRSF026649, MsbB, 1 hit |
TIGRFAMsi | TIGR02207, lipid_A_htrB, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ACV0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTNLPKFSTA LLHPRYWLTW LGIGVLWLVV QLPYPVIYRL GCGLGKLALR
60 70 80 90 100
FMKRRAKIVH RNLELCFPEM SEQERRKMVV KNFESVGMGL METGMAWFWP
110 120 130 140 150
DRRIARWTEV IGMEHIRDVQ AQKRGILLVG IHFLTLELGA RQFGMQEPGI
160 170 180 190 200
GVYRPNDNPL IDWLQTWGRL RSNKSMLDRK DLKGMIKALK KGEVVWYAPD
210 220 230 240 250
HDYGPRSSVF VPLFAVEQAA TTTGTWMLAR MSGACLVPFV PRRKPDGKGY
260 270 280 290 300
QLIMLPPECS PPLDDAETTA AWMNKVVEKC IMMAPEQYMW LHRRFKTRPE
GVPSRY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X61000 Genomic DNA Translation: CAA43317.1 U00096 Genomic DNA Translation: AAC74138.1 AP009048 Genomic DNA Translation: BAA35852.1 X59939 Genomic DNA No translation available. |
PIRi | S16888 |
RefSeqi | NP_415572.1, NC_000913.3 WP_000183364.1, NZ_STEB01000016.1 |
Genome annotation databases
EnsemblBacteriai | AAC74138; AAC74138; b1054 BAA35852; BAA35852; BAA35852 |
GeneIDi | 48135189 946216 |
KEGGi | ecj:JW1041 eco:b1054 |
PATRICi | fig|511145.12.peg.1095 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X61000 Genomic DNA Translation: CAA43317.1 U00096 Genomic DNA Translation: AAC74138.1 AP009048 Genomic DNA Translation: BAA35852.1 X59939 Genomic DNA No translation available. |
PIRi | S16888 |
RefSeqi | NP_415572.1, NC_000913.3 WP_000183364.1, NZ_STEB01000016.1 |
3D structure databases
SMRi | P0ACV0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262842, 71 interactors |
IntActi | P0ACV0, 9 interactors |
STRINGi | 511145.b1054 |
Proteomic databases
jPOSTi | P0ACV0 |
PaxDbi | P0ACV0 |
PRIDEi | P0ACV0 |
Genome annotation databases
EnsemblBacteriai | AAC74138; AAC74138; b1054 BAA35852; BAA35852; BAA35852 |
GeneIDi | 48135189 946216 |
KEGGi | ecj:JW1041 eco:b1054 |
PATRICi | fig|511145.12.peg.1095 |
Organism-specific databases
EchoBASEi | EB0459 |
Phylogenomic databases
eggNOGi | COG1560, Bacteria |
HOGENOMi | CLU_049421_1_1_6 |
InParanoidi | P0ACV0 |
PhylomeDBi | P0ACV0 |
Enzyme and pathway databases
UniPathwayi | UPA00030 UPA00360;UER00485 |
BioCyci | EcoCyc:LAUROYLACYLTRAN-MONOMER MetaCyc:LAUROYLACYLTRAN-MONOMER |
BRENDAi | 2.3.1.B29, 2026 |
Miscellaneous databases
PROi | PR:P0ACV0 |
Family and domain databases
CDDi | cd07984, LPLAT_LABLAT-like, 1 hit |
HAMAPi | MF_01942, Lipid_A_LpxL_LpxP, 1 hit |
InterProi | View protein in InterPro IPR004960, LipA_acyltrans IPR011920, Lipid_A_LpxL_LpxP |
PANTHERi | PTHR30606, PTHR30606, 1 hit |
Pfami | View protein in Pfam PF03279, Lip_A_acyltrans, 1 hit |
PIRSFi | PIRSF026649, MsbB, 1 hit |
TIGRFAMsi | TIGR02207, lipid_A_htrB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LPXL_ECOLI | |
Accessioni | P0ACV0Primary (citable) accession number: P0ACV0 Secondary accession number(s): P24187 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | November 22, 2005 | |
Last modified: | December 2, 2020 | |
This is version 113 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families