fabR

Functionⁱ

Binds the promoter region of at least fabA and fabB, but probably not yqfA (PubMed:11160901, PubMed:19854834, PubMed:21276098). Represses the transcription of fabA and fabB, involved in unsaturated fatty acid (UFA) biosynthesis (PubMed:11859088). By controlling UFA production, FabR directly influences the physical properties of the membrane bilayer.4 Publications

Miscellaneous

Probably part of the fabR-yijD operon.1 Publication

Caution

It is uncertain whether Met-1 or Met-20 is the initiator.Curated

Activity regulationⁱ

Has been suggested to require either an unsaturated acyl carrier protein or unsaturated acyl-CoA (but not their saturated equivalents) for DNA-binding (PubMed:19854834). Another group suggests that unsaturated thioesters are not essential but act instead to enhance DNA-binding (PubMed:21276098).2 Publications

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
DNA bindingⁱ	52 – 71	H-T-H motifPROSITE-ProRule annotationAdd BLAST		20

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

fatty acid biosynthetic process Source: UniProtKB-KW
negative regulation of transcription, DNA-templated
Source: EcoCyc
Inferred from direct assayⁱ
- 11160901
- 11859088
transcription, DNA-templated Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Repressor
Biological process	Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transcription, Transcription regulation

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG11394-MONOMER

BioCycⁱ

EcoCyc:EG11394-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: HTH-type transcriptional repressor FabR1 Publication
Gene namesⁱ	Name:fabR1 Publication Synonyms:yijC Ordered Locus Names:b3963, JW3935
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11394 fabR

EcoGeneⁱ

EG11394 fabR

Subcellular locationⁱ

Cytoplasm Curated

GO - Cellular componentⁱ

cytosol Source: GO_Central

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Significantly increased levels of unsaturated fatty acids (UFA) (PubMed:11859088, PubMed:19854834). Double fadR-fabR deletions have increased levels of saturated fatty acids, suggesting a functional fadR gene is required for fabR function (PubMed:11859088). Increased transcription of fabA and fabB (PubMed:11859088, PubMed:21276098).3 Publications

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000070641	1 – 234	HTH-type transcriptional repressor FabRAdd BLAST		234

Proteomic databases

PaxDbⁱ	P0ACU5
PRIDEⁱ	P0ACU5

Interactionⁱ

Subunit structureⁱ

Homodimer.2 Publications

Protein-protein interaction databases

BioGridⁱ	4261883, 156 interactors
Database of interacting proteins More...DIPⁱ	DIP-12520N
STRINGⁱ	316385.ECDH10B_4152

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P0ACU5
SMRⁱ	P0ACU5
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	29 – 89	HTH tetR-typePROSITE-ProRule annotationAdd BLAST		61

Phylogenomic databases

eggNOGⁱ	ENOG4105EJ2 Bacteria COG1309 LUCA
HOGENOMⁱ	HOG000270364
InParanoidⁱ	P0ACU5
KEGG Orthology (KO) More...KOⁱ	K22105

Family and domain databases

HAMAPⁱ	MF_01190 HTH_type_FabR, 1 hit
InterProⁱ	View protein in InterPro IPR009057 Homeobox-like_sf IPR001647 HTH_TetR IPR023764 Tscrpt_reg_HTH_FabR
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00440 TetR_N, 1 hit
SUPFAMⁱ	SSF46689 SSF46689, 1 hit
PROSITEⁱ	View protein in PROSITE PS50977 HTH_TETR_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0ACU5-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MFILWYSASS TFGKDSDIVM GVRAQQKEKT RRSLVEAAFS QLSAERSFAS 
        60         70         80         90        100
LSLREVAREA GIAPTSFYRH FRDVDELGLT MVDESGLMLR QLMRQARQRI 
       110        120        130        140        150
AKGGSVIRTS VSTFMEFIGN NPNAFRLLLR ERSGTSAAFR AAVAREIQHF 
       160        170        180        190        200
IAELADYLEL ENHMPRAFTE AQAEAMVTIV FSAGAEALDV GVEQRRQLEE 
       210        220        230 
RLVLQLRMIS KGAYYWYRRE QEKTAIIPGN VKDE

Length:234

Mass (Da):26,553

Last modified:November 2, 2016 - v2

Checksum:ⁱ326336C26008274E

GO

Sequence cautionⁱ

The sequence AAC76945 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	47	S → T in CAA46823 (PubMed:1447162).Curated	1
Sequence conflictⁱ	61	G → V in CAA46823 (PubMed:1447162).Curated	1
Sequence conflictⁱ	88 – 89	ML → IV in CAA46823 (PubMed:1447162).Curated	2
Sequence conflictⁱ	93 – 94	MR → IG in CAA46823 (PubMed:1447162).Curated	2

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱ	61	G → V in strain K12 / BW25113, may not bind DNA. 1 Publication		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X66026 Genomic DNA Translation: CAA46823.1 U00006 Genomic DNA Translation: AAC43069.1 U00096 Genomic DNA Translation: AAC76945.2 Different initiation. AP009048 Genomic DNA Translation: BAE77348.1
NCBI Reference Sequences More...RefSeqⁱ	NP_418398.2, NC_000913.3

Genome annotation databases

EnsemblBacteriaⁱ	AAC76945; AAC76945; b3963 BAE77348; BAE77348; BAE77348
GeneIDⁱ	948460
KEGGⁱ	ecj:JW3935 eco:b3963
PATRICⁱ	fig\|1411691.4.peg.2741

Protein	Similar proteins		Species	Score	Length	Source
P0ACU5	HTH-type transcriptional repressor FabR		ECO24		215	UniRef100_P0ACU5
HTH-type transcriptional repressor FabR		ECO57		215
HTH-type transcriptional repressor FabR		ECODH		215
HTH-type transcriptional repressor FabR		SHIDS		215
HTH-type transcriptional repressor FabR		SHIF8		215
+146

Protein	Similar proteins		Species	Score	Length	Source
P0ACU5	HTH-type transcriptional repressor FabR		ECO24		215	UniRef90_P0ACU5
HTH-type transcriptional repressor FabR		ECO57		215
HTH-type transcriptional repressor FabR		ECODH		215
HTH-type transcriptional repressor FabR		SHIDS		215
HTH-type transcriptional repressor FabR		SHIF8		215
+242

Protein	Similar proteins		Species	Score	Length	Source
P0ACU5	HTH-type transcriptional repressor FabR		ECO24		215	UniRef50_P0ACU5
HTH-type transcriptional repressor FabR		ECO57		215
HTH-type transcriptional repressor FabR		ECODH		215
HTH-type transcriptional repressor FabR		SHIDS		215
HTH-type transcriptional repressor FabR		SHIF8		215
+258

Entry informationⁱ

Entry nameⁱ	FABR_ECOLI
Accessionⁱ	P0ACU5Primary (citable) accession number: P0ACU5 Secondary accession number(s): P27307, Q2M8Q8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
	Last sequence update:	November 2, 2016
	Last modified:	September 12, 2018
	This is version 98 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

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UniRef

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Annotation systems

Supporting data

UniProtKB - P0ACU5 (FABR_ECOLI)

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HTH-type transcriptional repressor FabR

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Caution

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence cautionⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ