UniProtKB - P0ACU5 (FABR_ECOLI)
Protein
HTH-type transcriptional repressor FabR
Gene
fabR
Organism
Escherichia coli (strain K12)
Status
Functioni
Binds the promoter region of at least fabA and fabB, but probably not yqfA (PubMed:11160901, PubMed:19854834, PubMed:21276098). Represses the transcription of fabA and fabB, involved in unsaturated fatty acid (UFA) biosynthesis (PubMed:11859088). By controlling UFA production, FabR directly influences the physical properties of the membrane bilayer.4 Publications
Miscellaneous
Probably part of the fabR-yijD operon.1 Publication
Caution
It is uncertain whether Met-1 or Met-20 is the initiator.Curated
Activity regulationi
Has been suggested to require either an unsaturated acyl carrier protein or unsaturated acyl-CoA (but not their saturated equivalents) for DNA-binding (PubMed:19854834). Another group suggests that unsaturated thioesters are not essential but act instead to enhance DNA-binding (PubMed:21276098).2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 52 – 71 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-binding transcription factor activity Source: EcoCyc
GO - Biological processi
- fatty acid biosynthetic process Source: UniProtKB-UniRule
- negative regulation of fatty acid biosynthetic process Source: UniProtKB-UniRule
- negative regulation of transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11394-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional repressor FabR1 Publication |
Gene namesi | Name:fabR1 Publication Synonyms:yijC Ordered Locus Names:b3963, JW3935 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Significantly increased levels of unsaturated fatty acids (UFA) (PubMed:11859088, PubMed:19854834). Double fadR-fabR deletions have increased levels of saturated fatty acids, suggesting a functional fadR gene is required for fabR function (PubMed:11859088). Increased transcription of fabA and fabB (PubMed:11859088, PubMed:21276098).3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070641 | 1 – 234 | HTH-type transcriptional repressor FabRAdd BLAST | 234 |
Proteomic databases
PaxDbi | P0ACU5 |
PRIDEi | P0ACU5 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4261883, 156 interactors |
DIPi | DIP-12520N |
STRINGi | 511145.b3963 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 89 | HTH tetR-typePROSITE-ProRule annotationAdd BLAST | 61 |
Phylogenomic databases
eggNOGi | COG1309, Bacteria |
HOGENOMi | CLU_081861_0_0_6 |
InParanoidi | P0ACU5 |
Family and domain databases
HAMAPi | MF_01190, HTH_type_FabR, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR001647, HTH_TetR IPR023764, Tscrpt_reg_HTH_FabR |
Pfami | View protein in Pfam PF00440, TetR_N, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS50977, HTH_TETR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ACU5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFILWYSASS TFGKDSDIVM GVRAQQKEKT RRSLVEAAFS QLSAERSFAS
60 70 80 90 100
LSLREVAREA GIAPTSFYRH FRDVDELGLT MVDESGLMLR QLMRQARQRI
110 120 130 140 150
AKGGSVIRTS VSTFMEFIGN NPNAFRLLLR ERSGTSAAFR AAVAREIQHF
160 170 180 190 200
IAELADYLEL ENHMPRAFTE AQAEAMVTIV FSAGAEALDV GVEQRRQLEE
210 220 230
RLVLQLRMIS KGAYYWYRRE QEKTAIIPGN VKDE
Sequence cautioni
The sequence AAC76945 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 47 | S → T in CAA46823 (PubMed:1447162).Curated | 1 | |
Sequence conflicti | 61 | G → V in CAA46823 (PubMed:1447162).Curated | 1 | |
Sequence conflicti | 88 – 89 | ML → IV in CAA46823 (PubMed:1447162).Curated | 2 | |
Sequence conflicti | 93 – 94 | MR → IG in CAA46823 (PubMed:1447162).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 61 | G → V in strain K12 / BW25113, may not bind DNA. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66026 Genomic DNA Translation: CAA46823.1 U00006 Genomic DNA Translation: AAC43069.1 U00096 Genomic DNA Translation: AAC76945.2 Different initiation. AP009048 Genomic DNA Translation: BAE77348.1 |
RefSeqi | NP_418398.2, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAC76945; AAC76945; b3963 BAE77348; BAE77348; BAE77348 |
GeneIDi | 948460 |
KEGGi | ecj:JW3935 eco:b3963 |
PATRICi | fig|1411691.4.peg.2741 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66026 Genomic DNA Translation: CAA46823.1 U00006 Genomic DNA Translation: AAC43069.1 U00096 Genomic DNA Translation: AAC76945.2 Different initiation. AP009048 Genomic DNA Translation: BAE77348.1 |
RefSeqi | NP_418398.2, NC_000913.3 |
3D structure databases
SMRi | P0ACU5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261883, 156 interactors |
DIPi | DIP-12520N |
STRINGi | 511145.b3963 |
Proteomic databases
PaxDbi | P0ACU5 |
PRIDEi | P0ACU5 |
Genome annotation databases
EnsemblBacteriai | AAC76945; AAC76945; b3963 BAE77348; BAE77348; BAE77348 |
GeneIDi | 948460 |
KEGGi | ecj:JW3935 eco:b3963 |
PATRICi | fig|1411691.4.peg.2741 |
Organism-specific databases
EchoBASEi | EB1367 |
Phylogenomic databases
eggNOGi | COG1309, Bacteria |
HOGENOMi | CLU_081861_0_0_6 |
InParanoidi | P0ACU5 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11394-MONOMER |
Miscellaneous databases
PROi | PR:P0ACU5 |
Family and domain databases
HAMAPi | MF_01190, HTH_type_FabR, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR001647, HTH_TetR IPR023764, Tscrpt_reg_HTH_FabR |
Pfami | View protein in Pfam PF00440, TetR_N, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS50977, HTH_TETR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FABR_ECOLI | |
Accessioni | P0ACU5Primary (citable) accession number: P0ACU5 Secondary accession number(s): P27307, Q2M8Q8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | November 2, 2016 | |
Last modified: | December 2, 2020 | |
This is version 113 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |