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UniProtKB - P0ACT4 (TETR4_ECOLX)
Protein
Tetracycline repressor protein class D
Gene
tetR
Organism
Escherichia coli
Status
Functioni
TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 64 | TetracyclineCombined sources1 Publication | 1 | |
Binding sitei | 82 | TetracyclineCombined sources1 Publication | 1 | |
Metal bindingi | 100 | MagnesiumCombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 26 – 45 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- negative regulation of transcription, DNA-templated Source: InterPro
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Antibiotic resistance, Transcription, Transcription regulation |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Tetracycline repressor protein class D |
Gene namesi | Name:tetR |
Encoded oni | Plasmid RA1 |
Organismi | Escherichia coli |
Taxonomic identifieri | 562 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000070615 | 2 – 218 | Tetracycline repressor protein class DAdd BLAST | 217 |
Expressioni
Inductioni
By the [Mg-tetracycline]+ complex.
Interactioni
Subunit structurei
Homodimer.
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0ACT4 |
SASBDBi | P0ACT4 |
SMRi | P0ACT4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ACT4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 63 | HTH tetR-typePROSITE-ProRule annotationAdd BLAST | 61 |
Family and domain databases
InterProi | View protein in InterPro IPR023772, DNA-bd_HTH_TetR-type_CS IPR009057, Homeobox-like_sf IPR001647, HTH_TetR IPR004111, Repressor_TetR_C IPR003012, Tet_transcr_reg_TetR IPR036271, Tet_transcr_reg_TetR-rel_C_sf |
Pfami | View protein in Pfam PF02909, TetR_C_1, 1 hit PF00440, TetR_N, 1 hit |
PRINTSi | PR00455, HTHTETR PR00400, TETREPRESSOR |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF48498, SSF48498, 1 hit |
PROSITEi | View protein in PROSITE PS01081, HTH_TETR_1, 1 hit PS50977, HTH_TETR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0ACT4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARLNRESVI DAALELLNET GIDGLTTRKL AQKLGIEQPT LYWHVKNKRA
60 70 80 90 100
LLDALAVEIL ARHHDYSLPA AGESWQSFLR NNAMSFRRAL LRYRDGAKVH
110 120 130 140 150
LGTRPDEKQY DTVETQLRFM TENGFSLRDG LYAISAVSHF TLGAVLEQQE
160 170 180 190 200
HTAALTDRPA APDENLPPLL REALQIMDSD DGEQAFLHGL ESLIRGFEVQ
210
LTALLQIVGG DKLIIPFC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X01083 Genomic DNA Translation: CAA25561.1 |
PIRi | S07359 |
RefSeqi | WP_000113282.1, NZ_WSXE01000114.1 YP_002995714.1, NC_012886.1 YP_007349483.1, NC_020086.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X01083 Genomic DNA Translation: CAA25561.1 |
PIRi | S07359 |
RefSeqi | WP_000113282.1, NZ_WSXE01000114.1 YP_002995714.1, NC_012886.1 YP_007349483.1, NC_020086.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A6I | X-ray | 2.40 | A | 6-218 | [»] | |
1BJZ | X-ray | 2.20 | A | 3-208 | [»] | |
1ORK | X-ray | 2.40 | A | 3-208 | [»] | |
1QPI | X-ray | 2.50 | A | 4-206 | [»] | |
2FJ1 | X-ray | 2.20 | A | 2-208 | [»] | |
2NS7 | X-ray | 2.40 | A/B/C/D | 188-208 | [»] | |
2NS8 | X-ray | 2.55 | A/B/C/D | 188-208 | [»] | |
2O7O | X-ray | 1.89 | A | 2-208 | [»] | |
2TCT | X-ray | 2.10 | A | 2-208 | [»] | |
2TRT | X-ray | 2.50 | A | 2-218 | [»] | |
2VKE | X-ray | 1.62 | A | 3-208 | [»] | |
2VKV | X-ray | 1.74 | A | 51-208 | [»] | |
2X6O | X-ray | 2.30 | A | 3-208 | [»] | |
2X9D | X-ray | 2.51 | A | 3-208 | [»] | |
2XB5 | X-ray | 2.50 | A | 2-208 | [»] | |
2XGC | X-ray | 2.15 | A/B | 51-208 | [»] | |
2XGD | X-ray | 2.25 | A | 51-208 | [»] | |
2XGE | X-ray | 2.14 | A/B | 51-208 | [»] | |
2XPU | X-ray | 1.55 | A | 3-208 | [»] | |
2XPV | X-ray | 1.49 | A | 3-208 | [»] | |
2XPW | X-ray | 1.44 | A | 3-208 | [»] | |
2XRL | X-ray | 1.85 | A | 3-208 | [»] | |
3FK6 | X-ray | 2.10 | A/B | 51-208 | [»] | |
3FK7 | X-ray | 2.06 | A/B | 51-208 | [»] | |
3ZQF | X-ray | 2.56 | A | 188-208 | [»] | |
3ZQG | X-ray | 2.45 | A | 188-208 | [»] | |
3ZQH | X-ray | 1.60 | A | 188-208 | [»] | |
3ZQI | X-ray | 1.50 | A/B | 188-208 | [»] | |
4ABZ | X-ray | 1.89 | A | 3-208 | [»] | |
4AUX | X-ray | 2.25 | A | 2-208 | [»] | |
4B1R | X-ray | 1.95 | A | 3-208 | [»] | |
4B3A | X-ray | 1.70 | A | 3-208 | [»] | |
4D7M | X-ray | 1.75 | A | 3-208 | [»] | |
4D7N | X-ray | 1.89 | A | 3-208 | [»] | |
4V2F | X-ray | 2.40 | A | 3-208 | [»] | |
4V2G | X-ray | 2.70 | A/B | 3-208 | [»] | |
5FKK | X-ray | 1.75 | A/B | 3-208 | [»] | |
5FKL | X-ray | 1.90 | A | 3-208 | [»] | |
5FKM | X-ray | 1.63 | A | 3-208 | [»] | |
5FKN | X-ray | 1.80 | A/B | 3-208 | [»] | |
5FKO | X-ray | 1.85 | A | 3-208 | [»] | |
6FPL | X-ray | 1.60 | A | 2-208 | [»] | |
6FPM | X-ray | 1.85 | A | 2-208 | [»] | |
6FTS | X-ray | 2.00 | A | 3-208 | [»] | |
6QJW | X-ray | 2.10 | A | 2-208 | [»] | |
6QJX | X-ray | 2.10 | A | 2-208 | [»] | |
6RBL | X-ray | 1.90 | A | 2-208 | [»] | |
6RBM | X-ray | 2.05 | A | 2-208 | [»] | |
6RCR | X-ray | 2.05 | A | 2-208 | [»] | |
6RGX | X-ray | 1.80 | A/B | 3-208 | [»] | |
6YR1 | X-ray | 2.30 | AAA | 3-208 | [»] | |
6YR2 | X-ray | 1.95 | AAA/BBB | 2-208 | [»] | |
AlphaFoldDBi | P0ACT4 | |||||
SASBDBi | P0ACT4 | |||||
SMRi | P0ACT4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ACT4 |
Family and domain databases
InterProi | View protein in InterPro IPR023772, DNA-bd_HTH_TetR-type_CS IPR009057, Homeobox-like_sf IPR001647, HTH_TetR IPR004111, Repressor_TetR_C IPR003012, Tet_transcr_reg_TetR IPR036271, Tet_transcr_reg_TetR-rel_C_sf |
Pfami | View protein in Pfam PF02909, TetR_C_1, 1 hit PF00440, TetR_N, 1 hit |
PRINTSi | PR00455, HTHTETR PR00400, TETREPRESSOR |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF48498, SSF48498, 1 hit |
PROSITEi | View protein in PROSITE PS01081, HTH_TETR_1, 1 hit PS50977, HTH_TETR_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TETR4_ECOLX | |
Accessioni | P0ACT4Primary (citable) accession number: P0ACT4 Secondary accession number(s): P09164 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 99 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, PlasmidDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references