UniProtKB - P0ACP7 (PURR_ECOLI)
HTH-type transcriptional repressor PurR
purR
Functioni
Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. In addition, it participates in the regulation or coregulation of genes involved in de novo pyrimidine nucleotide biosynthesis, salvage and uptake (pyrC, pyrD, carAB and codBA), and of several genes encoding enzymes necessary for nucleotide and polyamine biosynthesis (prsA, glyA, gcvTHP, speA, glnB). Binds to a 16-bp palindromic sequence located within the promoter region of pur regulon genes. The consensus binding sequence is 5'-ACGCAAACGTTTTCNT-3'. PurR is allosterically activated to bind its cognate DNA by binding the purine corepressors, hypoxanthine or guanine, thereby effecting transcription repression.
4 PublicationsMiscellaneous
: purine nucleotide biosynthesis Pathwayi
This protein regulates the pathway purine nucleotide biosynthesis, which is part of Purine metabolism.View all proteins of this organism that are known to be involved in the pathway purine nucleotide biosynthesis and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 73 | HypoxanthineCombined sources3 Publications | 1 | |
Binding sitei | 190 | HypoxanthineCombined sources3 Publications | 1 | |
Sitei | 190 | Is the key determinant of 6-oxopurine corepressor specificity | 1 | |
Binding sitei | 192 | HypoxanthineCombined sources3 Publications | 1 | |
Binding sitei | 221 | HypoxanthineCombined sources3 Publications | 1 | |
Binding sitei | 275 | HypoxanthineCombined sources3 Publications | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 4 – 23 | H-T-H motifAdd BLAST | 20 | |
DNA bindingi | 48 – 56 | 9 |
GO - Molecular functioni
- DNA-binding transcription factor activity Source: GO_Central
- DNA-binding transcription repressor activity Source: EcoCyc
- guanine binding Source: EcoCyc
- transcription cis-regulatory region binding Source: GO_Central
GO - Biological processi
- negative regulation of purine nucleotide biosynthetic process Source: EcoCyc
- negative regulation of transcription, DNA-templated Source: EcoCyc
- purine nucleotide biosynthetic process Source: UniProtKB-UniPathway
- regulation of transcription, DNA-templated Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Purine biosynthesis, Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00219 |
UniPathwayi | UPA00488 |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional repressor PurRAlternative name(s): Pur regulon repressor Purine nucleotide synthesis repressor |
Gene namesi | Name:purR Ordered Locus Names:b1658, JW1650 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 54 | L → M: Slight decrease in operator DNA affinity. | 1 | |
Mutagenesisi | 55 | K → A: Decrease in operator DNA affinity. 1 Publication | 1 | |
Mutagenesisi | 147 | W → A: 14-fold increase in corepressor affinity. Large increase in repressor activity. 1 Publication | 1 | |
Mutagenesisi | 147 | W → F: Large decrease in corepressor affinity and in repressor activity. 1 Publication | 1 | |
Mutagenesisi | 147 | W → R: 8-fold increase in corepressor affinity. Large increase in repressor activity. 1 Publication | 1 | |
Mutagenesisi | 190 | R → A: Functional repressor. Corepressor specificity is expanded since it allows binding of adenine and 6-methylpurine. 1 Publication | 1 | |
Mutagenesisi | 190 | R → Q: Functional repressor. Corepressor specificity is expanded since it allows binding of adenine. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000107976 | 2 – 341 | HTH-type transcriptional repressor PurRAdd BLAST | 340 |
Proteomic databases
jPOSTi | P0ACP7 |
PaxDbi | P0ACP7 |
PRIDEi | P0ACP7 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homodimer.
6 PublicationsBinary interactionsi
P0ACP7
With | #Exp. | IntAct |
---|---|---|
yebV [P64503] | 4 | EBI-1115258,EBI-9126792 |
Protein-protein interaction databases
BioGRIDi | 4260271, 7 interactors 849611, 1 interactor |
DIPi | DIP-35931N |
IntActi | P0ACP7, 8 interactors |
STRINGi | 511145.b1658 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P0ACP7 |
SMRi | P0ACP7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ACP7 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 56 | HTH lacI-typeAdd BLAST | 55 |
Domaini
Phylogenomic databases
eggNOGi | COG1609, Bacteria |
HOGENOMi | CLU_037628_6_2_6 |
InParanoidi | P0ACP7 |
OMAi | ARWVGPP |
PhylomeDBi | P0ACP7 |
Family and domain databases
CDDi | cd01392, HTH_LacI, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
HAMAPi | MF_01277, HTH_type_PurR, 1 hit |
InterProi | View protein in InterPro IPR000843, HTH_LacI IPR046335, LacI/GalR-like_sensor IPR010982, Lambda_DNA-bd_dom_sf IPR028082, Peripla_BP_I IPR023588, Tscrpt_reg_HTH_PurR |
Pfami | View protein in Pfam PF00356, LacI, 1 hit PF13377, Peripla_BP_3, 1 hit |
PRINTSi | PR00036, HTHLACI |
SMARTi | View protein in SMART SM00354, HTH_LACI, 1 hit |
SUPFAMi | SSF47413, SSF47413, 1 hit SSF53822, SSF53822, 1 hit |
PROSITEi | View protein in PROSITE PS00356, HTH_LACI_1, 1 hit PS50932, HTH_LACI_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MATIKDVAKR ANVSTTTVSH VINKTRFVAE ETRNAVWAAI KELHYSPSAV
60 70 80 90 100
ARSLKVNHTK SIGLLATSSE AAYFAEIIEA VEKNCFQKGY TLILGNAWNN
110 120 130 140 150
LEKQRAYLSM MAQKRVDGLL VMCSEYPEPL LAMLEEYRHI PMVVMDWGEA
160 170 180 190 200
KADFTDAVID NAFEGGYMAG RYLIERGHRE IGVIPGPLER NTGAGRLAGF
210 220 230 240 250
MKAMEEAMIK VPESWIVQGD FEPESGYRAM QQILSQPHRP TAVFCGGDIM
260 270 280 290 300
AMGALCAADE MGLRVPQDVS LIGYDNVRNA RYFTPALTTI HQPKDSLGET
310 320 330 340
AFNMLLDRIV NKREEPQSIE VHPRLIERRS VADGPFRDYR R
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04212 Genomic DNA Translation: AAA24457.1 X51368 Genomic DNA Translation: CAA35753.1 U00096 Genomic DNA Translation: AAC74730.1 AP009048 Genomic DNA Translation: BAA15424.1 |
PIRi | A32027, RPECDU |
RefSeqi | NP_416175.1, NC_000913.3 WP_000190982.1, NZ_STEB01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC74730; AAC74730; b1658 BAA15424; BAA15424; BAA15424 |
GeneIDi | 66674449 945226 |
KEGGi | ecj:JW1650 eco:b1658 |
PATRICi | fig|1411691.4.peg.599 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04212 Genomic DNA Translation: AAA24457.1 X51368 Genomic DNA Translation: CAA35753.1 U00096 Genomic DNA Translation: AAC74730.1 AP009048 Genomic DNA Translation: BAA15424.1 |
PIRi | A32027, RPECDU |
RefSeqi | NP_416175.1, NC_000913.3 WP_000190982.1, NZ_STEB01000003.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BDH | X-ray | 2.70 | A | 2-341 | [»] | |
1BDI | X-ray | 3.00 | A | 2-341 | [»] | |
1DBQ | X-ray | 2.20 | A/B | 53-341 | [»] | |
1JFS | X-ray | 2.90 | A | 2-341 | [»] | |
1JFT | X-ray | 2.50 | A | 2-341 | [»] | |
1JH9 | X-ray | 2.55 | A | 2-341 | [»] | |
1JHZ | X-ray | 2.40 | A/B | 53-341 | [»] | |
1PNR | X-ray | 2.70 | A | 2-341 | [»] | |
1PRU | NMR | - | A | 1-56 | [»] | |
1PRV | NMR | - | A | 1-56 | [»] | |
1QP0 | X-ray | 2.90 | A | 2-341 | [»] | |
1QP4 | X-ray | 3.00 | A | 2-341 | [»] | |
1QP7 | X-ray | 2.90 | A | 2-341 | [»] | |
1QPZ | X-ray | 2.50 | A | 2-341 | [»] | |
1QQA | X-ray | 3.00 | A | 2-341 | [»] | |
1QQB | X-ray | 2.70 | A | 2-341 | [»] | |
1VPW | X-ray | 2.70 | A | 2-341 | [»] | |
1WET | X-ray | 2.60 | A | 2-341 | [»] | |
1ZAY | X-ray | 2.70 | A | 2-341 | [»] | |
2PUA | X-ray | 2.90 | A | 2-341 | [»] | |
2PUB | X-ray | 2.70 | A | 2-341 | [»] | |
2PUC | X-ray | 2.60 | A | 2-341 | [»] | |
2PUD | X-ray | 2.60 | A | 2-341 | [»] | |
2PUE | X-ray | 2.70 | A | 2-341 | [»] | |
2PUF | X-ray | 3.00 | A | 2-341 | [»] | |
2PUG | X-ray | 2.70 | A | 2-341 | [»] | |
AlphaFoldDBi | P0ACP7 | |||||
SMRi | P0ACP7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260271, 7 interactors 849611, 1 interactor |
DIPi | DIP-35931N |
IntActi | P0ACP7, 8 interactors |
STRINGi | 511145.b1658 |
Proteomic databases
jPOSTi | P0ACP7 |
PaxDbi | P0ACP7 |
PRIDEi | P0ACP7 |
Genome annotation databases
EnsemblBacteriai | AAC74730; AAC74730; b1658 BAA15424; BAA15424; BAA15424 |
GeneIDi | 66674449 945226 |
KEGGi | ecj:JW1650 eco:b1658 |
PATRICi | fig|1411691.4.peg.599 |
Organism-specific databases
EchoBASEi | EB0793 |
Phylogenomic databases
eggNOGi | COG1609, Bacteria |
HOGENOMi | CLU_037628_6_2_6 |
InParanoidi | P0ACP7 |
OMAi | ARWVGPP |
PhylomeDBi | P0ACP7 |
Enzyme and pathway databases
UniPathwayi | UPA00488 |
BioCyci | EcoCyc:PD00219 |
Miscellaneous databases
EvolutionaryTracei | P0ACP7 |
PROi | PR:P0ACP7 |
Family and domain databases
CDDi | cd01392, HTH_LacI, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
HAMAPi | MF_01277, HTH_type_PurR, 1 hit |
InterProi | View protein in InterPro IPR000843, HTH_LacI IPR046335, LacI/GalR-like_sensor IPR010982, Lambda_DNA-bd_dom_sf IPR028082, Peripla_BP_I IPR023588, Tscrpt_reg_HTH_PurR |
Pfami | View protein in Pfam PF00356, LacI, 1 hit PF13377, Peripla_BP_3, 1 hit |
PRINTSi | PR00036, HTHLACI |
SMARTi | View protein in SMART SM00354, HTH_LACI, 1 hit |
SUPFAMi | SSF47413, SSF47413, 1 hit SSF53822, SSF53822, 1 hit |
PROSITEi | View protein in PROSITE PS00356, HTH_LACI_1, 1 hit PS50932, HTH_LACI_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PURR_ECOLI | |
Accessioni | P0ACP7Primary (citable) accession number: P0ACP7 Secondary accession number(s): P15039, Q83RB1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 128 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references