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UniProtKB - P0ACN4 (ALLR_ECOLI)
Protein
HTH-type transcriptional repressor AllR
Gene
allR
Organism
Escherichia coli (strain K12)
Status
Functioni
Negative regulator of allantoin and glyoxylate utilization operons. Binds to the gcl promoter and to the allS-allA intergenic region. Binding to DNA is abolished by glyoxylate.
3 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 207 | Glyoxylate | 1 | |
Binding sitei | 217 | Glyoxylate | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 43 – 62 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: GO_Central
- DNA-binding transcription factor activity Source: EcoCyc
- identical protein binding Source: IntAct
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
- negative regulation of transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:G6276-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional repressor AllRAlternative name(s): Negative regulator of allantoin and glyoxylate utilization operons |
Gene namesi | Name:allR Synonyms:glxA3, ybbU Ordered Locus Names:b0506, JW0494 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 135 – 142 | CKSMVRMC → APA: Loss of repressor activity. Loss of DNA binding. Reduces assembly into functionally active tetramers. 1 Publication | 8 | |
Mutagenesisi | 141 | M → A: No effect on repressor activity. Loss of glyoxylate-triggered dissociation from DNA. 1 Publication | 1 | |
Mutagenesisi | 142 | C → W: Strongly reduced repressor activity. Reduces DNA binding. Prevents assembly into functionally active tetramers. 1 Publication | 1 | |
Mutagenesisi | 215 | L → A: Reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. 1 Publication | 1 | |
Mutagenesisi | 217 | C → A or S: Slightly reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. 1 Publication | 1 | |
Mutagenesisi | 234 | S → A or N: Reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. 1 Publication | 1 | |
Mutagenesisi | 236 | S → A or M: Slightly reduced repressor activity. Loss of glyoxylate-triggered dissociation from DNA. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201753 | 1 – 271 | HTH-type transcriptional repressor AllRAdd BLAST | 271 |
Proteomic databases
jPOSTi | P0ACN4 |
PaxDbi | P0ACN4 |
PRIDEi | P0ACN4 |
Interactioni
Subunit structurei
Homotetramer and homodimer. Homotetramer in its active, DNA-bound form. Homodimer in its inactive form.
1 PublicationBinary interactionsi
P0ACN4
With | #Exp. | IntAct |
---|---|---|
itself | 4 | EBI-561736,EBI-561736 |
caiC [P31552] | 3 | EBI-561736,EBI-1112975 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259871, 7 interactors 849709, 2 interactors |
DIPi | DIP-47907N |
IntActi | P0ACN4, 21 interactors |
STRINGi | 511145.b0506 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0ACN4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ACN4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 83 | HTH iclR-typePROSITE-ProRule annotationAdd BLAST | 63 | |
Domaini | 98 – 267 | IclR-EDPROSITE-ProRule annotationAdd BLAST | 170 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 154 – 156 | Glyoxylate binding | 3 | |
Regioni | 234 – 236 | Glyoxylate binding | 3 |
Phylogenomic databases
eggNOGi | COG1414, Bacteria |
HOGENOMi | CLU_062618_7_1_6 |
InParanoidi | P0ACN4 |
PhylomeDBi | P0ACN4 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 3.30.450.40, 1 hit |
InterProi | View protein in InterPro IPR029016, GAF-like_dom_sf IPR014757, Tscrpt_reg_IclR_C IPR005471, Tscrpt_reg_IclR_N IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF09339, HTH_IclR, 1 hit PF01614, IclR, 1 hit |
SMARTi | View protein in SMART SM00346, HTH_ICLR, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS51077, HTH_ICLR, 1 hit PS51078, ICLR_ED, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ACN4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTEVRRRGRP GQAEPVAQKG AQALERGIAI LQYLEKSGGS SSVSDISLNL
60 70 80 90 100
DLPLSTTFRL LKVLQAADFV YQDSQLGWWH IGLGVFNVGA AYIHNRDVLS
110 120 130 140 150
VAGPFMRRLM LLSGETVNVA IRNGNEAVLI GQLECKSMVR MCAPLGSRLP
160 170 180 190 200
LHASGAGKAL LYPLAEEELM SIILQTGLQQ FTPTTLVDMP TLLKDLEQAR
210 220 230 240 250
ELGYTVDKEE HVVGLNCIAS AIYDDVGSVV AAISISGPSS RLTEDRFVSQ
260 270
GELVRDTARD ISTALGLKAH P
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U89024 Genomic DNA Translation: AAB93847.1 U82664 Genomic DNA Translation: AAB40259.1 U00096 Genomic DNA Translation: AAC73608.1 AP009048 Genomic DNA Translation: BAE76284.1 |
PIRi | A64782 |
RefSeqi | NP_415039.1, NC_000913.3 WP_000141275.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73608; AAC73608; b0506 BAE76284; BAE76284; BAE76284 |
GeneIDi | 67416416 945333 |
KEGGi | ecj:JW0494 eco:b0506 |
PATRICi | fig|1411691.4.peg.1771 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U89024 Genomic DNA Translation: AAB93847.1 U82664 Genomic DNA Translation: AAB40259.1 U00096 Genomic DNA Translation: AAC73608.1 AP009048 Genomic DNA Translation: BAE76284.1 |
PIRi | A64782 |
RefSeqi | NP_415039.1, NC_000913.3 WP_000141275.1, NZ_STEB01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1TF1 | X-ray | 1.80 | A/B/C/D | 97-271 | [»] | |
SMRi | P0ACN4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259871, 7 interactors 849709, 2 interactors |
DIPi | DIP-47907N |
IntActi | P0ACN4, 21 interactors |
STRINGi | 511145.b0506 |
Proteomic databases
jPOSTi | P0ACN4 |
PaxDbi | P0ACN4 |
PRIDEi | P0ACN4 |
Genome annotation databases
EnsemblBacteriai | AAC73608; AAC73608; b0506 BAE76284; BAE76284; BAE76284 |
GeneIDi | 67416416 945333 |
KEGGi | ecj:JW0494 eco:b0506 |
PATRICi | fig|1411691.4.peg.1771 |
Organism-specific databases
EchoBASEi | EB3382 |
Phylogenomic databases
eggNOGi | COG1414, Bacteria |
HOGENOMi | CLU_062618_7_1_6 |
InParanoidi | P0ACN4 |
PhylomeDBi | P0ACN4 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6276-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0ACN4 |
PROi | PR:P0ACN4 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 3.30.450.40, 1 hit |
InterProi | View protein in InterPro IPR029016, GAF-like_dom_sf IPR014757, Tscrpt_reg_IclR_C IPR005471, Tscrpt_reg_IclR_N IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF09339, HTH_IclR, 1 hit PF01614, IclR, 1 hit |
SMARTi | View protein in SMART SM00346, HTH_ICLR, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS51077, HTH_ICLR, 1 hit PS51078, ICLR_ED, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ALLR_ECOLI | |
Accessioni | P0ACN4Primary (citable) accession number: P0ACN4 Secondary accession number(s): P77734, Q2MBS2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | November 22, 2005 | |
Last modified: | February 23, 2022 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references