UniProtKB - P0ACI0 (ROB_ECOLI)

BLAST

>sp|P0ACI0|ROB_ECOLI Right origin-binding protein OS=Escherichia coli (strain K12) OX=83333 GN=rob PE=1 SV=1
MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLS
KSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRL
GEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYG
LNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFI
LTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRPINLRCELLIPIRR

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History
Entry version 106 (12 Sep 2018)
Sequence version 1 (22 Nov 2005)
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Protein

Right origin-binding protein

Gene

rob

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
DNA bindingⁱ	24 – 43	H-T-H motifPROSITE-ProRule annotationAdd BLAST		20

GO - Molecular functionⁱ

DNA-binding transcription factor activity Source: InterPro
glyoxalase III activity Source: GO_Central
sequence-specific DNA binding
Source: EcoliWiki
Inferred from direct assayⁱ
- 10551881

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: GO_Central

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding

Molecular function

DNA-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:PD04418

BioCycⁱ

EcoCyc:PD04418

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Right origin-binding protein
Gene namesⁱ	Name:rob Ordered Locus Names:b4396, JW4359
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11366 rob

EcoGeneⁱ

EG11366 rob

Subcellular locationⁱ

GO - Cellular componentⁱ

cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 18304323

View the complete GO annotation on QuickGO ...

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000194578	1 – 289	Right origin-binding proteinAdd BLAST		289

Proteomic databases

PaxDbⁱ	P0ACI0
PRIDEⁱ	P0ACI0

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4259321, 108 interactors
Database of interacting proteins More...DIPⁱ	DIP-47868N
IntActⁱ	P0ACI0, 17 interactors
STRINGⁱ	316385.ECDH10B_4554

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	4 – 15	Combined sources	12
Beta strandⁱ	18 – 21	Combined sources	4
Helixⁱ	25 – 28	Combined sources	4
Turnⁱ	29 – 31	Combined sources	3
Helixⁱ	35 – 46	Combined sources	12
Helixⁱ	50 – 68	Combined sources	19
Helixⁱ	73 – 79	Combined sources	7
Helixⁱ	85 – 96	Combined sources	12
Helixⁱ	100 – 105	Combined sources	6
Beta strandⁱ	107 – 110	Combined sources	4
Beta strandⁱ	128 – 133	Combined sources	6
Beta strandⁱ	136 – 144	Combined sources	9
Helixⁱ	149 – 151	Combined sources	3
Helixⁱ	152 – 170	Combined sources	19
Beta strandⁱ	178 – 186	Combined sources	9
Beta strandⁱ	188 – 190	Combined sources	3
Beta strandⁱ	194 – 203	Combined sources	10
Helixⁱ	204 – 207	Combined sources	4
Helixⁱ	208 – 210	Combined sources	3
Beta strandⁱ	215 – 219	Combined sources	5
Beta strandⁱ	222 – 232	Combined sources	11
Helixⁱ	233 – 235	Combined sources	3
Helixⁱ	236 – 245	Combined sources	10
Helixⁱ	247 – 250	Combined sources	4
Beta strandⁱ	260 – 264	Combined sources	5
Helixⁱ	266 – 268	Combined sources	3
Beta strandⁱ	278 – 288	Combined sources	11

Show more details

3D structure databases

ProteinModelPortalⁱ	P0ACI0
SMRⁱ	P0ACI0
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P0ACI0

Family & Domainsⁱ

Phylogenomic databases

eggNOGⁱ	ENOG4105UEI Bacteria COG2207 LUCA
HOGENOMⁱ	HOG000127886
InParanoidⁱ	P0ACI0
KEGG Orthology (KO) More...KOⁱ	K05804
OMAⁱ	FWRQYLG
PhylomeDBⁱ	P0ACI0

Family and domain databases

Gene3Dⁱ	3.20.80.10, 1 hit
InterProⁱ	View protein in InterPro IPR010499 AraC_E-bd IPR029442 GyrI-like IPR009057 Homeobox-like_sf IPR018060 HTH_AraC IPR018062 HTH_AraC-typ_CS IPR011256 Reg_factor_effector_dom_sf IPR020449 Tscrpt_reg_HTH_AraC-type
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06445 GyrI-like, 1 hit PF12833 HTH_18, 1 hit
PRINTSⁱ	PR00032 HTHARAC
SMARTⁱ	View protein in SMART SM00871 AraC_E_bind, 1 hit SM00342 HTH_ARAC, 1 hit
SUPFAMⁱ	SSF46689 SSF46689, 2 hits SSF55136 SSF55136, 1 hit
PROSITEⁱ	View protein in PROSITE PS00041 HTH_ARAC_FAMILY_1, 1 hit PS01124 HTH_ARAC_FAMILY_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0ACI0-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDQAGIIRDL LIWLEGHLDQ PLSLDNVAAK AGYSKWHLQR MFKDVTGHAI 
        60         70         80         90        100
GAYIRARRLS KSAVALRLTA RPILDIALQY RFDSQQTFTR AFKKQFAQTP 
       110        120        130        140        150
ALYRRSPEWS AFGIRPPLRL GEFTMPEHKF VTLEDTPLIG VTQSYSCSLE 
       160        170        180        190        200
QISDFRHEMR YQFWHDFLGN APTIPPVLYG LNETRPSQDK DDEQEVFYTT 
       210        220        230        240        250
ALAQDQADGY VLTGHPVMLQ GGEYVMFTYE GLGTGVQEFI LTVYGTCMPM 
       260        270        280 
LNLTRRKGQD IERYYPAEDA KAGDRPINLR CELLIPIRR

Length:289

Mass (Da):33,145

Last modified:November 22, 2005 - v1

Checksum:ⁱ4436B1A04BE86A93

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M97495 Genomic DNA Translation: AAA24569.1 U14003 Genomic DNA Translation: AAA97292.1 U00096 Genomic DNA Translation: AAC77349.1 AP009048 Genomic DNA Translation: BAE78385.1
PIRⁱ	JU0158
NCBI Reference Sequences More...RefSeqⁱ	NP_418813.1, NC_000913.3 WP_000371666.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC77349; AAC77349; b4396 BAE78385; BAE78385; BAE78385
GeneIDⁱ	948916
KEGGⁱ	ecj:JW4359 eco:b4396
PATRICⁱ	fig\|1411691.4.peg.2288

Protein	Similar proteins		Species	Score	Length	Source
P0ACI0	Right origin-binding protein		ECO57		289	UniRef100_P0ACI1
Right origin-binding protein		SHIFL		289
GyrI-like and HTH 18 domain containing protein		TRITR		289
AraC-family transcriptional regulator		uncultured Escherichia sp.		289
GyrI-like small molecule binding domain protein		uncultured bacterium		289
+151

Protein	Similar proteins		Species	Score	Length	Source
P0ACI0	Right origin-binding protein		ECO57		289	UniRef90_P0ACI1
Right origin-binding protein		SHIFL		289
GyrI-like and HTH 18 domain containing protein		TRITR		289
AraC-family transcriptional regulator		uncultured Escherichia sp.		289
GyrI-like small molecule binding domain protein		uncultured bacterium		289
+672

Protein	Similar proteins		Species	Score	Length	Source
P0ACI0	Right origin-binding protein		ECO57		289	UniRef50_P0ACI1
Right origin-binding protein		SHIFL		289
GyrI-like and HTH 18 domain containing protein		TRITR		289
AraC-family transcriptional regulator		uncultured Escherichia sp.		289
GyrI-like small molecule binding domain protein		uncultured bacterium		289
+1685

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M97495 Genomic DNA Translation: AAA24569.1 U14003 Genomic DNA Translation: AAA97292.1 U00096 Genomic DNA Translation: AAC77349.1 AP009048 Genomic DNA Translation: BAE78385.1
PIRⁱ	JU0158
RefSeqⁱ	NP_418813.1, NC_000913.3 WP_000371666.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1D5Y	X-ray	2.70	A/B/C/D	3-289	[»]
ProteinModelPortalⁱ	P0ACI0
SMRⁱ	P0ACI0
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4259321, 108 interactors
DIPⁱ	DIP-47868N
IntActⁱ	P0ACI0, 17 interactors
STRINGⁱ	316385.ECDH10B_4554

Proteomic databases

PaxDbⁱ	P0ACI0
PRIDEⁱ	P0ACI0

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC77349; AAC77349; b4396 BAE78385; BAE78385; BAE78385
GeneIDⁱ	948916
KEGGⁱ	ecj:JW4359 eco:b4396
PATRICⁱ	fig\|1411691.4.peg.2288

Organism-specific databases

EchoBASEⁱ	EB1340
EcoGeneⁱ	EG11366 rob

Phylogenomic databases

eggNOGⁱ	ENOG4105UEI Bacteria COG2207 LUCA
HOGENOMⁱ	HOG000127886
InParanoidⁱ	P0ACI0
KOⁱ	K05804
OMAⁱ	FWRQYLG
PhylomeDBⁱ	P0ACI0

Enzyme and pathway databases

BioCycⁱ	EcoCyc:PD04418

BioCycⁱ

EcoCyc:PD04418

Miscellaneous databases

EvolutionaryTraceⁱ	P0ACI0
Protein Ontology More...PROⁱ	PR:P0ACI0

Family and domain databases

Gene3Dⁱ	3.20.80.10, 1 hit
InterProⁱ	View protein in InterPro IPR010499 AraC_E-bd IPR029442 GyrI-like IPR009057 Homeobox-like_sf IPR018060 HTH_AraC IPR018062 HTH_AraC-typ_CS IPR011256 Reg_factor_effector_dom_sf IPR020449 Tscrpt_reg_HTH_AraC-type
Pfamⁱ	View protein in Pfam PF06445 GyrI-like, 1 hit PF12833 HTH_18, 1 hit
PRINTSⁱ	PR00032 HTHARAC
SMARTⁱ	View protein in SMART SM00871 AraC_E_bind, 1 hit SM00342 HTH_ARAC, 1 hit
SUPFAMⁱ	SSF46689 SSF46689, 2 hits SSF55136 SSF55136, 1 hit
PROSITEⁱ	View protein in PROSITE PS00041 HTH_ARAC_FAMILY_1, 1 hit PS01124 HTH_ARAC_FAMILY_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ROB_ECOLI
Accessionⁱ	P0ACI0Primary (citable) accession number: P0ACI0 Secondary accession number(s): P27292, Q2M5S1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
	Last sequence update:	November 22, 2005
	Last modified:	September 12, 2018
	This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0ACI0 (ROB_ECOLI)

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Right origin-binding protein

rob

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ