UniProtKB - P0ACH8 (MELR_ECOLI)
Protein
Melibiose operon regulatory protein
Gene
melR
Organism
Escherichia coli (strain K12)
Status
Functioni
Transcription activator for the expression of the melAB operon. MelR binds at two sites located upstream of the melAB transcription site.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 211 – 232 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 22 | |
DNA bindingi | 259 – 282 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DNA binding Source: EcoCyc
- DNA-binding transcription factor activity Source: InterPro
- sequence-specific DNA binding Source: InterPro
GO - Biological processi
- regulation of transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00208 |
Names & Taxonomyi
Protein namesi | Recommended name: Melibiose operon regulatory protein |
Gene namesi | Name:melR Ordered Locus Names:b4118, JW4079 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 222 | N → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 269 | R → G: No effect. 1 Publication | 1 | |
Mutagenesisi | 271 | S → A: 50% reduced activity. 1 Publication | 1 | |
Mutagenesisi | 272 | S → N: 75% reduced activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194537 | 1 – 302 | Melibiose operon regulatory proteinAdd BLAST | 302 |
Proteomic databases
PaxDbi | P0ACH8 |
PRIDEi | P0ACH8 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262686, 12 interactors 852929, 5 interactors |
DIPi | DIP-10181N |
IntActi | P0ACH8, 15 interactors |
STRINGi | 511145.b4118 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 194 – 292 | HTH araC/xylS-typePROSITE-ProRule annotationAdd BLAST | 99 |
Phylogenomic databases
eggNOGi | COG2169, Bacteria |
HOGENOMi | CLU_000445_88_9_6 |
InParanoidi | P0ACH8 |
PhylomeDBi | P0ACH8 |
Family and domain databases
Gene3Di | 2.60.120.10, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR018060, HTH_AraC IPR018062, HTH_AraC-typ_CS IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf IPR008917, TF_DNA-bd_sf IPR020449, Tscrpt_reg_HTH_AraC-type |
Pfami | View protein in Pfam PF12833, HTH_18, 1 hit |
PRINTSi | PR00032, HTHARAC |
SMARTi | View protein in SMART SM00342, HTH_ARAC, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47454, SSF47454, 1 hit SSF51182, SSF51182, 1 hit |
PROSITEi | View protein in PROSITE PS00041, HTH_ARAC_FAMILY_1, 1 hit PS01124, HTH_ARAC_FAMILY_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ACH8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNTDTFMCSS DEKQTRSPLS LYSEYQRMEI EFRAPHIMPT SHWHGQVEVN
60 70 80 90 100
VPFDGDVEYL INNEKVNINQ GHITLFWACT PHQLTDTGTC QSMAIFNLPM
110 120 130 140 150
HLFLSWPLDK DLINHVTHGM VIKSLATQQL SPFEVRRWQQ ELNSPNEQIR
160 170 180 190 200
QLAIDEIGLM LKRFSLSGWE PILVNKTSRT HKNSVSRHAQ FYVSQMLGFI
210 220 230 240 250
AENYDQALTI NDVAEHVKLN ANYAMGIFQR VMQLTMKQYI TAMRINHVRA
260 270 280 290 300
LLSDTDKSIL DIALTAGFRS SSRFYSTFGK YVGMSPQQYR KLSQQRRQTF
PG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18425 Genomic DNA No translation available. U14003 Genomic DNA Translation: AAA97018.1 U00096 Genomic DNA Translation: AAC77079.1 AP009048 Genomic DNA Translation: BAE78120.1 |
PIRi | A29625, RGECMB |
RefSeqi | NP_418542.1, NC_000913.3 WP_001090758.1, NZ_STEB01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC77079; AAC77079; b4118 BAE78120; BAE78120; BAE78120 |
GeneIDi | 948637 |
KEGGi | ecj:JW4079 eco:b4118 |
PATRICi | fig|1411691.4.peg.2582 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18425 Genomic DNA No translation available. U14003 Genomic DNA Translation: AAA97018.1 U00096 Genomic DNA Translation: AAC77079.1 AP009048 Genomic DNA Translation: BAE78120.1 |
PIRi | A29625, RGECMB |
RefSeqi | NP_418542.1, NC_000913.3 WP_001090758.1, NZ_STEB01000014.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4262686, 12 interactors 852929, 5 interactors |
DIPi | DIP-10181N |
IntActi | P0ACH8, 15 interactors |
STRINGi | 511145.b4118 |
Proteomic databases
PaxDbi | P0ACH8 |
PRIDEi | P0ACH8 |
Genome annotation databases
EnsemblBacteriai | AAC77079; AAC77079; b4118 BAE78120; BAE78120; BAE78120 |
GeneIDi | 948637 |
KEGGi | ecj:JW4079 eco:b4118 |
PATRICi | fig|1411691.4.peg.2582 |
Organism-specific databases
EchoBASEi | EB1212 |
Phylogenomic databases
eggNOGi | COG2169, Bacteria |
HOGENOMi | CLU_000445_88_9_6 |
InParanoidi | P0ACH8 |
PhylomeDBi | P0ACH8 |
Enzyme and pathway databases
BioCyci | EcoCyc:PD00208 |
Miscellaneous databases
PROi | PR:P0ACH8 |
Family and domain databases
Gene3Di | 2.60.120.10, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR018060, HTH_AraC IPR018062, HTH_AraC-typ_CS IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf IPR008917, TF_DNA-bd_sf IPR020449, Tscrpt_reg_HTH_AraC-type |
Pfami | View protein in Pfam PF12833, HTH_18, 1 hit |
PRINTSi | PR00032, HTHARAC |
SMARTi | View protein in SMART SM00342, HTH_ARAC, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47454, SSF47454, 1 hit SSF51182, SSF51182, 1 hit |
PROSITEi | View protein in PROSITE PS00041, HTH_ARAC_FAMILY_1, 1 hit PS01124, HTH_ARAC_FAMILY_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MELR_ECOLI | |
Accessioni | P0ACH8Primary (citable) accession number: P0ACH8 Secondary accession number(s): P10411, Q2M6I6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | November 22, 2005 | |
Last modified: | December 2, 2020 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |