UniProtKB - P0ACG1 (STPA_ECOLI)
Protein
DNA-binding protein StpA
Gene
stpA
Organism
Escherichia coli (strain K12)
Status
Functioni
A DNA-binding protein that acts in a fashion similar to H-NS protein upon overexpression, represses a number of genes including the cryptic blg operon, hns, papB and the proU locus (PubMed:8890170). A subset of H-NS/StpA-regulated genes also require Hha for repression; Hha and Cnu (YdgT) increases the number of genes DNA bound by H-NS/StpA and may also modulate the oligomerization of the H-NS/StpA-complex (PubMed:23543115). Repression can be inhibited by dominant-negative mutants of StpA or H-NS (PubMed:8755860).3 Publications
(Microbial infection) Originally isolated as a suppressor of a splicing defect of the thymidylate synthase (td) gene from bacteriophage T4 (PubMed:1480493). Acts as an RNA chaperone, accelerating splicing of viral pre-mRNA. Binds preferentially to unstructured over structured RNA; does not have a detectable high-affinity RNA-binding site in the pre-mRNA. There do not seem to be any specific RNA targets in transcribed E.coli DNA (PubMed:17267410).2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 112 – 117 | By similarity | 6 |
GO - Molecular functioni
- bent DNA binding Source: GO_Central
- DNA binding Source: EcoliWiki
- DNA-binding transcription repressor activity Source: GO_Central
- minor groove of adenine-thymine-rich DNA binding Source: GO_Central
- protein dimerization activity Source: InterPro
- RNA binding Source: UniProtKB-KW
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- gene silencing Source: GO_Central
Keywordsi
Molecular function | Chaperone, DNA-binding, RNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11554-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-binding protein StpAAlternative name(s): H-NS homolog StpA |
Gene namesi | Name:stpA1 Publication Synonyms:hnsB Ordered Locus Names:b2669, JW2644 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytosol Source: EcoCyc
- nucleoid Source: UniProtKB-SubCell
- protein-DNA complex Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
No visible phenotype in rich medium; double hns-stpA mutants grow slower and have reduced viable cell counts compared to single hns mutants in MC1029 and MC4100 backgrounds (PubMed:8890170). In 0.3M NaCl a double hns-stpA deletion up-regulates 583 and down-regulates 86 genes, 363 of which are thought to have been horizontally acquired; 131 are also up-regulated in a double cnu-hha deletion (PubMed:23543115).2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 26 | L → P: Partial loss of repressor function when overexpressed in the absence of hns. 1 Publication | 1 | |
Mutagenesisi | 65 – 134 | Missing : Partial loss of repressor function when overexpressed in the absence of hns. 1 PublicationAdd BLAST | 70 | |
Mutagenesisi | 97 | Y → C: Partial loss of repressor function when overexpressed in the absence of hns. 1 Publication | 1 | |
Mutagenesisi | 116 | P → S: Partial loss of repressor function when overexpressed in the absence of hns. 1 Publication | 1 | |
Mutagenesisi | 126 | G → V: Higher RNA chaperone activity than wild-type, decreased ability to bind structured RNA. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000168513 | 1 – 134 | DNA-binding protein StpAAdd BLAST | 134 |
Proteomic databases
jPOSTi | P0ACG1 |
PaxDbi | P0ACG1 |
PRIDEi | P0ACG1 |
Expressioni
Inductioni
Expressed at low levels at 37 degrees Celsius, barely detectable at 26 degrees Celsius; expression is repressed by hns and partially activated by lrp (PubMed:8890170).1 Publication
Interactioni
Subunit structurei
When overexpressed forms homodimers, can interact with the N-terminus (residues 1-64) of H-NS (PubMed:8755860). May interact with Hha and/or Cnu.
Curated1 PublicationBinary interactionsi
P0ACG1
With | #Exp. | IntAct |
---|---|---|
hns [P0ACF8] | 4 | EBI-551928,EBI-544934 |
GO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 4262261, 8 interactors 851464, 2 interactors |
DIPi | DIP-35951N |
IntActi | P0ACG1, 24 interactors |
STRINGi | 511145.b2669 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0ACG1 |
SMRi | P0ACG1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 42 – 45 | Poly-Glu | 4 |
Domaini
Both the N-terminus (residues 1-76) and the C-terminus (residues 90-134) have RNA chaperone activity for splicing of td, a bacteriophage T4 pre-mRNA (PubMed:17267410).1 Publication
Sequence similaritiesi
Belongs to the histone-like protein H-NS family.Curated
Phylogenomic databases
eggNOGi | COG2916, Bacteria |
HOGENOMi | CLU_117503_0_0_6 |
Family and domain databases
Gene3Di | 1.10.287.1050, 1 hit 4.10.430.10, 1 hit |
InterProi | View protein in InterPro IPR027444, H-NS_C_dom IPR037150, H-NS_C_dom_sf IPR001801, Histone_HNS IPR027454, Histone_HNS_N |
Pfami | View protein in Pfam PF00816, Histone_HNS, 1 hit |
PIRSFi | PIRSF002096, HnS, 1 hit |
SMARTi | View protein in SMART SM00528, HNS, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ACG1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVMLQSLNN IRTLRAMARE FSIDVLEEML EKFRVVTKER REEEEQQQRE
60 70 80 90 100
LAERQEKIST WLELMKADGI NPEELLGNSS AAAPRAGKKR QPRPAKYKFT
110 120 130
DVNGETKTWT GQGRTPKPIA QALAEGKSLD DFLI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69210 Genomic DNA Translation: CAA49146.1 U07823 Genomic DNA Translation: AAA64940.1 U00096 Genomic DNA Translation: AAC75716.1 AP009048 Genomic DNA Translation: BAA16535.1 |
PIRi | JH0774 |
RefSeqi | NP_417155.1, NC_000913.3 WP_000115383.1, NZ_STEB01000042.1 |
Genome annotation databases
EnsemblBacteriai | AAC75716; AAC75716; b2669 BAA16535; BAA16535; BAA16535 |
GeneIDi | 48726518 947130 |
KEGGi | ecj:JW2644 eco:b2669 |
PATRICi | fig|1411691.4.peg.4072 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69210 Genomic DNA Translation: CAA49146.1 U07823 Genomic DNA Translation: AAA64940.1 U00096 Genomic DNA Translation: AAC75716.1 AP009048 Genomic DNA Translation: BAA16535.1 |
PIRi | JH0774 |
RefSeqi | NP_417155.1, NC_000913.3 WP_000115383.1, NZ_STEB01000042.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LRX | NMR | - | A | 90-134 | [»] | |
BMRBi | P0ACG1 | |||||
SMRi | P0ACG1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262261, 8 interactors 851464, 2 interactors |
DIPi | DIP-35951N |
IntActi | P0ACG1, 24 interactors |
STRINGi | 511145.b2669 |
Proteomic databases
jPOSTi | P0ACG1 |
PaxDbi | P0ACG1 |
PRIDEi | P0ACG1 |
Genome annotation databases
EnsemblBacteriai | AAC75716; AAC75716; b2669 BAA16535; BAA16535; BAA16535 |
GeneIDi | 48726518 947130 |
KEGGi | ecj:JW2644 eco:b2669 |
PATRICi | fig|1411691.4.peg.4072 |
Organism-specific databases
EchoBASEi | EB1515 |
Phylogenomic databases
eggNOGi | COG2916, Bacteria |
HOGENOMi | CLU_117503_0_0_6 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11554-MONOMER |
Miscellaneous databases
PROi | PR:P0ACG1 |
Family and domain databases
Gene3Di | 1.10.287.1050, 1 hit 4.10.430.10, 1 hit |
InterProi | View protein in InterPro IPR027444, H-NS_C_dom IPR037150, H-NS_C_dom_sf IPR001801, Histone_HNS IPR027454, Histone_HNS_N |
Pfami | View protein in Pfam PF00816, Histone_HNS, 1 hit |
PIRSFi | PIRSF002096, HnS, 1 hit |
SMARTi | View protein in SMART SM00528, HNS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | STPA_ECOLI | |
Accessioni | P0ACG1Primary (citable) accession number: P0ACG1 Secondary accession number(s): P30017 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 22, 2005 |
Last sequence update: | November 22, 2005 | |
Last modified: | December 2, 2020 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families