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Protein

DNA-binding protein HU-beta

Gene

hupB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct

GO - Biological processi

  • chromosome condensation Source: UniProtKB-KW
  • transcription, DNA-templated Source: EcoCyc

Keywordsi

Molecular functionDNA-binding
Biological processDNA condensation

Enzyme and pathway databases

BioCyciEcoCyc:EG10467-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein HU-beta
Alternative name(s):
HU-1
NS1
Gene namesi
Name:hupB
Synonyms:hopD
Ordered Locus Names:b0440, JW0430
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10467 hupB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30A → D: No effect. 1 Publication1
Mutagenesisi37K → Q: No effect. 1 Publication1
Mutagenesisi47F → T: Reduced DNA-binding. 1 Publication1
Mutagenesisi58 – 61RTGR → GTGG: Reduced DNA-binding. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001049391 – 90DNA-binding protein HU-betaAdd BLAST90

Proteomic databases

EPDiP0ACF4
PaxDbiP0ACF4
PRIDEiP0ACF4

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4260733, 44 interactors
ComplexPortaliCPX-1958 HU complex
CPX-1960 HU complex variant 2
CPX-1961 DnaA-HU complex
DIPiDIP-31833N
IntActiP0ACF4, 77 interactors
STRINGi316385.ECDH10B_0396

Structurei

Secondary structure

190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 14Combined sources12
Helixi18 – 37Combined sources20
Beta strandi42 – 44Combined sources3
Turni45 – 47Combined sources3
Beta strandi48 – 54Combined sources7
Beta strandi75 – 81Combined sources7
Helixi83 – 88Combined sources6

3D structure databases

ProteinModelPortaliP0ACF4
SMRiP0ACF4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACF4

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105K70 Bacteria
COG0776 LUCA
HOGENOMiHOG000043828
InParanoidiP0ACF4
KOiK03530
OMAiRSNATKA
PhylomeDBiP0ACF4

Family and domain databases

Gene3Di4.10.520.10, 1 hit
InterProiView protein in InterPro
IPR000119 Hist_DNA-bd
IPR020816 Histone-like_DNA-bd_CS
IPR010992 IHF-like_DNA-bd_dom_sf
PANTHERiPTHR33175 PTHR33175, 1 hit
PfamiView protein in Pfam
PF00216 Bac_DNA_binding, 1 hit
PRINTSiPR01727 DNABINDINGHU
SMARTiView protein in SMART
SM00411 BHL, 1 hit
SUPFAMiSSF47729 SSF47729, 1 hit
PROSITEiView protein in PROSITE
PS00045 HISTONE_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

P0ACF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKSQLIDKI AAGADISKAA AGRALDAIIA SVTESLKEGD DVALVGFGTF
60 70 80 90
AVKERAARTG RNPQTGKEIT IAAAKVPSFR AGKALKDAVN
Length:90
Mass (Da):9,226
Last modified:July 21, 1986 - v1
Checksum:iC56A3C766D53BB96
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12A → E AA sequence (PubMed:350619).Curated1
Sequence conflicti16I → L AA sequence (PubMed:350619).Curated1
Sequence conflicti26D → E AA sequence (PubMed:350619).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16540 Genomic DNA Translation: CAA34539.1
U82664 Genomic DNA Translation: AAB40196.1
U00096 Genomic DNA Translation: AAC73543.1
AP009048 Genomic DNA Translation: BAE76220.1
X53241 Genomic DNA Translation: CAA37332.1
D82943 Genomic DNA Translation: BAA11644.1
PIRiS06880 DNECS1
RefSeqiNP_414974.1, NC_000913.3
WP_001043542.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73543; AAC73543; b0440
BAE76220; BAE76220; BAE76220
GeneIDi949095
KEGGiecj:JW0430
eco:b0440
PATRICifig|1411691.4.peg.1836

Similar proteinsi

Entry informationi

Entry nameiDBHB_ECOLI
AccessioniPrimary (citable) accession number: P0ACF4
Secondary accession number(s): P02341, Q2MBY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 20, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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