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Entry version 105 (05 Jun 2019)
Sequence version 1 (22 Nov 2005)
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Protein

Hydrogenase-1 large chain

Gene

hyaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Ni2+By similarityNote: Binds 1 nickel ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi76NickelSequence analysis1
Metal bindingi79NickelSequence analysis1
Metal bindingi576NickelSequence analysis1
Metal bindingi579NickelSequence analysis1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, Nickel

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:HYAB-MONOMER
ECOL316407:JW0955-MONOMER
MetaCyc:HYAB-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
1.12.99.6 2026

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Hydrogenase-1 large chain (EC:1.12.99.6)
Short name:
HYD1
Alternative name(s):
Membrane-bound hydrogenase 1 large subunit
NiFe hydrogenase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:hyaB
Ordered Locus Names:b0973, JW0955
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

Escherichia coli strain K12 genome database

More...
EcoGenei
EG10469 hyaB

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001997121 – 597Hydrogenase-1 large chainAdd BLAST597

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P0ACD8

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P0ACD8

PRoteomics IDEntifications database

More...
PRIDEi
P0ACD8

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Heterodimer of a large and a small subunit.

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4260043, 18 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-281 Hydrogenase-1 complex

Database of interacting proteins

More...
DIPi
DIP-36180N

Protein interaction database and analysis system

More...
IntActi
P0ACD8, 10 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b0973

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1597
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P0ACD8

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105DPX Bacteria
COG0374 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000278799

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P0ACD8

KEGG Orthology (KO)

More...
KOi
K06281

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P0ACD8

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.645.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR001501 Ni-dep_hyd_lsu
IPR018194 Ni-dep_hyd_lsu_Ni_BS
IPR029014 NiFe-Hase_large

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00374 NiFeSe_Hases, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF56762 SSF56762, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00507 NI_HGENASE_L_1, 1 hit
PS00508 NI_HGENASE_L_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0ACD8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTQYETQGY TINNAGRRLV VDPITRIEGH MRCEVNINDQ NVITNAVSCG
60 70 80 90 100
TMFRGLEIIL QGRDPRDAWA FVERICGVCT GVHALASVYA IEDAIGIKVP
110 120 130 140 150
DNANIIRNIM LATLWCHDHL VHFYQLAGMD WIDVLDALKA DPRKTSELAQ
160 170 180 190 200
SLSSWPKSSP GYFFDVQNRL KKFVEGGQLG IFRNGYWGHP QYKLPPEANL
210 220 230 240 250
MGFAHYLEAL DFQREIVKIH AVFGGKNPHP NWIVGGMPCA INIDESGAVG
260 270 280 290 300
AVNMERLNLV QSIITRTADF INNVMIPDAL AIGQFNKPWS EIGTGLSDKC
310 320 330 340 350
VLSYGAFPDI ANDFGEKSLL MPGGAVINGD FNNVLPVDLV DPQQVQEFVD
360 370 380 390 400
HAWYRYPNDQ VGRHPFDGIT DPWYNPGDVK GSDTNIQQLN EQERYSWIKA
410 420 430 440 450
PRWRGNAMEV GPLARTLIAY HKGDAATVES VDRMMSALNL PLSGIQSTLG
460 470 480 490 500
RILCRAHEAQ WAAGKLQYFF DKLMTNLKNG NLATASTEKW EPATWPTECR
510 520 530 540 550
GVGFTEAPRG ALGHWAAIRD GKIDLYQCVV PTTWNASPRD PKGQIGAYEA
560 570 580 590
ALMNTKMAIP EQPLEILRTL HSFDPCLACS THVLGDDGSE LISVQVR
Length:597
Mass (Da):66,253
Last modified:November 22, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i659DD7EDE16D6342
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti52M → I in AAA23998 (PubMed:2180913).Curated1
Sequence conflicti69W → R in AAA23998 (PubMed:2180913).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M34825 Genomic DNA Translation: AAA23998.1
U00096 Genomic DNA Translation: AAC74058.1
AP009048 Genomic DNA Translation: BAA35738.2

Protein sequence database of the Protein Information Resource

More...
PIRi
C64838 HQECL

NCBI Reference Sequences

More...
RefSeqi
NP_415492.1, NC_000913.3
WP_000107384.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC74058; AAC74058; b0973
BAA35738; BAA35738; BAA35738

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
945580

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW0955
eco:b0973

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.1301

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34825 Genomic DNA Translation: AAA23998.1
U00096 Genomic DNA Translation: AAC74058.1
AP009048 Genomic DNA Translation: BAA35738.2
PIRiC64838 HQECL
RefSeqiNP_415492.1, NC_000913.3
WP_000107384.1, NZ_LN832404.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UQYX-ray1.47L/M1-582[»]
3USCX-ray2.00L/M1-582[»]
3USEX-ray1.67L/M1-582[»]
4GD3X-ray3.30J/K/L/M1-582[»]
4UE3X-ray1.40L/M1-582[»]
5A4FX-ray1.25L/M1-582[»]
5A4IX-ray1.23L/M1-582[»]
5A4MX-ray1.70L/M1-582[»]
5ADUX-ray1.10L/M1-582[»]
5JRDX-ray1.20L/M1-582[»]
5LMMX-ray1.20L/M1-582[»]
5LRYX-ray1.40L/M1-582[»]
6FPIX-ray1.50L/M1-582[»]
6FPOX-ray1.05L/M1-582[»]
6FPWX-ray1.35L/M1-582[»]
6G7RX-ray1.20L/M1-582[»]
6G94X-ray2.50J/K/L/M1-582[»]
6GALX-ray1.25L/M1-582[»]
SMRiP0ACD8
ModBaseiSearch...

Protein-protein interaction databases

BioGridi4260043, 18 interactors
ComplexPortaliCPX-281 Hydrogenase-1 complex
DIPiDIP-36180N
IntActiP0ACD8, 10 interactors
STRINGi511145.b0973

Proteomic databases

jPOSTiP0ACD8
PaxDbiP0ACD8
PRIDEiP0ACD8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74058; AAC74058; b0973
BAA35738; BAA35738; BAA35738
GeneIDi945580
KEGGiecj:JW0955
eco:b0973
PATRICifig|1411691.4.peg.1301

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0464
EcoGeneiEG10469 hyaB

Phylogenomic databases

eggNOGiENOG4105DPX Bacteria
COG0374 LUCA
HOGENOMiHOG000278799
InParanoidiP0ACD8
KOiK06281
PhylomeDBiP0ACD8

Enzyme and pathway databases

BioCyciEcoCyc:HYAB-MONOMER
ECOL316407:JW0955-MONOMER
MetaCyc:HYAB-MONOMER
BRENDAi1.12.99.6 2026

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P0ACD8

Family and domain databases

Gene3Di1.10.645.10, 1 hit
InterProiView protein in InterPro
IPR001501 Ni-dep_hyd_lsu
IPR018194 Ni-dep_hyd_lsu_Ni_BS
IPR029014 NiFe-Hase_large
PfamiView protein in Pfam
PF00374 NiFeSe_Hases, 1 hit
SUPFAMiSSF56762 SSF56762, 1 hit
PROSITEiView protein in PROSITE
PS00507 NI_HGENASE_L_1, 1 hit
PS00508 NI_HGENASE_L_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMBHL_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0ACD8
Secondary accession number(s): P19927, P78056
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: June 5, 2019
This is version 105 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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