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Protein

Iron-sulfur cluster assembly scaffold protein IscU

Gene

iscU

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A scaffold on which IscS assembles Fe-S clusters. Exists as 2 interconverting forms, a structured (S) and disordered (D) form. The D-state is the preferred substrate for IscS. Converts to the S-state when an Fe-S cluster is assembled, which helps it dissociate from IscS to transfer the Fe-S to an acceptor. It is likely that Fe-S cluster coordination is flexible as the role of this complex is to build and then hand off Fe-S clusters.2 Publications

Activity regulationi

Carboxymethylation by iodoacetic acid blocks sulfur transfer to this protein.1 Publication

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: EcoCyc
  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • ferrous iron binding Source: GO_Central
  • identical protein binding Source: IntAct
  • iron-sulfur transferase activity Source: GO_Central

GO - Biological processi

Enzyme and pathway databases

BioCyciEcoCyc:G7324-MONOMER
MetaCyc:G7324-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-sulfur cluster assembly scaffold protein IscU
Alternative name(s):
Sulfur acceptor protein IscU
Gene namesi
Name:iscU
Synonyms:nifU, yfhN
Ordered Locus Names:b2529, JW2513
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13395 iscU

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39D → A, L or V: Stabilizes apo-protein in the S-state (structured). 2 Publications1
Mutagenesisi39D → G: No effect on equilibrium between S- and D-state (disordered). 2 Publications1
Mutagenesisi89K → A: Stabilizes apo-protein in the D-state; Fe-S cluster assembly 3-fold slower than wild-type. 1 Publication1
Mutagenesisi90N → A: Stabilizes apo-protein in the S-state; biphasic Fe-S cluster assembly 7-fold slower than wild-type. 2 Publications1
Mutagenesisi90N → D: Stabilizes apo-protein in the D-state; Fe-S cluster assembly 3-fold slower than wild-type. 2 Publications1
Mutagenesisi107S → A: Stabilizes apo-protein in the S-state; biphasic Fe-S cluster assembly 7-fold slower than wild-type. 2 Publications1
Mutagenesisi111E → A: Stabilizes apo-protein in the S-state; biphasic Fe-S cluster assembly 7-fold slower than wild-type. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001661841 – 128Iron-sulfur cluster assembly scaffold protein IscUAdd BLAST128

Proteomic databases

EPDiP0ACD4
PaxDbiP0ACD4
PRIDEiP0ACD4

Interactioni

Subunit structurei

Homodimer. Forms a heterotetramer with IscS; each subunit of the IscS dimer contacts an IscU monomer.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4259432, 63 interactors
851341, 1 interactor
ComplexPortaliCPX-2141 IscS-IscU complex
DIPiDIP-48025N
IntActiP0ACD4, 17 interactors
STRINGi316385.ECDH10B_2696

Structurei

Secondary structure

1128
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0ACD4
SMRiP0ACD4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACD4

Family & Domainsi

Sequence similaritiesi

Belongs to the NifU family.Curated

Phylogenomic databases

eggNOGiENOG4107XTC Bacteria
COG0822 LUCA
HOGENOMiHOG000069228
InParanoidiP0ACD4
KOiK04488
PhylomeDBiP0ACD4

Family and domain databases

CDDicd06664 IscU_like, 1 hit
InterProiView protein in InterPro
IPR011339 ISC_FeS_clus_asmbl_IscU
IPR002871 NIF_FeS_clus_asmbl_NifU_N
PANTHERiPTHR10093 PTHR10093, 1 hit
PfamiView protein in Pfam
PF01592 NifU_N, 1 hit
TIGRFAMsiTIGR01999 iscU, 1 hit

Sequencei

Sequence statusi: Complete.

P0ACD4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAYSEKVIDH YENPRNVGSF DNNDENVGSG MVGAPACGDV MKLQIKVNDE
60 70 80 90 100
GIIEDARFKT YGCGSAIASS SLVTEWVKGK SLDEAQAIKN TDIAEELELP
110 120
PVKIHCSILA EDAIKAAIAD YKSKREAK
Length:128
Mass (Da):13,849
Last modified:November 22, 2005 - v1
Checksum:i47516021442B6535
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75582.1
AP009048 Genomic DNA Translation: BAA16423.1
PIRiH65029
RefSeqiNP_417024.1, NC_000913.3
WP_000331707.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75582; AAC75582; b2529
BAA16423; BAA16423; BAA16423
GeneIDi947002
KEGGiecj:JW2513
eco:b2529
PATRICifig|1411691.4.peg.4205

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75582.1
AP009048 Genomic DNA Translation: BAA16423.1
PIRiH65029
RefSeqiNP_417024.1, NC_000913.3
WP_000331707.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KQKNMR-A1-128[»]
2L4XNMR-A1-128[»]
ProteinModelPortaliP0ACD4
SMRiP0ACD4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259432, 63 interactors
851341, 1 interactor
ComplexPortaliCPX-2141 IscS-IscU complex
DIPiDIP-48025N
IntActiP0ACD4, 17 interactors
STRINGi316385.ECDH10B_2696

Proteomic databases

EPDiP0ACD4
PaxDbiP0ACD4
PRIDEiP0ACD4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75582; AAC75582; b2529
BAA16423; BAA16423; BAA16423
GeneIDi947002
KEGGiecj:JW2513
eco:b2529
PATRICifig|1411691.4.peg.4205

Organism-specific databases

EchoBASEiEB3176
EcoGeneiEG13395 iscU

Phylogenomic databases

eggNOGiENOG4107XTC Bacteria
COG0822 LUCA
HOGENOMiHOG000069228
InParanoidiP0ACD4
KOiK04488
PhylomeDBiP0ACD4

Enzyme and pathway databases

BioCyciEcoCyc:G7324-MONOMER
MetaCyc:G7324-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0ACD4
PROiPR:P0ACD4

Family and domain databases

CDDicd06664 IscU_like, 1 hit
InterProiView protein in InterPro
IPR011339 ISC_FeS_clus_asmbl_IscU
IPR002871 NIF_FeS_clus_asmbl_NifU_N
PANTHERiPTHR10093 PTHR10093, 1 hit
PfamiView protein in Pfam
PF01592 NifU_N, 1 hit
TIGRFAMsiTIGR01999 iscU, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiISCU_ECOLI
AccessioniPrimary (citable) accession number: P0ACD4
Secondary accession number(s): P77310
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: November 7, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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