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UniProtKB - P0ACC1 (PRMC_ECOLI)

Entry version 120 (07 Apr 2021)
Sequence version 1 (08 Nov 2005)
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Protein

Release factor glutamine methyltransferase

Gene

prmC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif, i.e. on 'Gln-235' in RF1 and on 'Gln-252' in RF2.3 Publications

Caution

Was originally thought to be involved in the oxidation of protoporphyrinogen into protoporphyrin IX.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei140S-adenosyl-L-methionine2 Publications1
Binding sitei168S-adenosyl-L-methionine2 Publications1
Binding sitei183S-adenosyl-L-methionine1 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:EG12424-MONOMER
MetaCyc:EG12424-MONOMER

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.1.1.297, 2026

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Release factor glutamine methyltransferaseUniRule annotation (EC:2.1.1.2972 Publications)
Short name:
RF MTaseUniRule annotation
Alternative name(s):
M.EcoKHemKP
N5-glutamine methyltransferase PrmCUniRule annotation
Protein release factor methylation C
Protein-(glutamine-N5) MTase PrmCUniRule annotation
Protein-glutamine N-methyltransferase PrmCUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:prmC
Synonyms:hemK
Ordered Locus Names:b1212, JW1203
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Cells lacking this gene suffer severe growth defects, but also show a global shift in gene expression to anaerobic respiration. Also shows defects in translational termination via an enhanced rate of read-through of nonsense codons and induction of transfer-mRNA-mediated tagging of proteins within the cell.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi36R → A: 17% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi40L → A: 60% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi41A → R: 7% of wild-type RF1 methylation activity. Strongly reduced affinity for S-adenosyl-L-methionine. 1 Publication1
Mutagenesisi42F → A: 40% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi44E → A: 62% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi44E → R: 33% of wild-type RF1 methylation activity. 1 Publication1
Mutagenesisi183N → A: Less than 2% of wild-type RF1 methylation activity. 1 Publication1

Chemistry databases

Drug and drug target database

More...DrugBanki		DB01752, S-adenosyl-L-homocysteine

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001571561 – 277Release factor glutamine methyltransferaseAdd BLAST277

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P0ACC1

PRoteomics IDEntifications database

More...PRIDEi		P0ACC1

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with PrfA and PrfB.

2 Publications

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4260113, 35 interactors

Protein interaction database and analysis system

More...IntActi		P0ACC1, 1 interactor

STRING: functional protein association networks

More...STRINGi		511145.b1212

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1277
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P0ACC1

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P0ACC1

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni117 – 121S-adenosyl-L-methionine bindingUniRule annotation1 Publication5
Regioni183 – 186Substrate binding1 Publication4

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG2890, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_018398_3_1_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P0ACC1

Database for complete collections of gene phylogenies

More...PhylomeDBi		P0ACC1

Family and domain databases

HAMAP database of protein families

More...HAMAPi		MF_02126, RF_methyltr_PrmC, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR002052, DNA_methylase_N6_adenine_CS
IPR004556, HemK-like
IPR025714, Methyltranfer_dom
IPR040758, PrmC_N
IPR019874, Release_fac_Glu-N5_MeTfrase
IPR029063, SAM-dependent_MTases

Pfam protein domain database

More...Pfami		View protein in Pfam
PF13847, Methyltransf_31, 1 hit
PF17827, PrmC_N, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF53335, SSF53335, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00536, hemK_fam, 1 hit
TIGR03534, RF_mod_PrmC, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0ACC1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEYQHWLREA ISQLQASESP RRDAEILLEH VTGRGRTFIL AFGETQLTDE 
        60         70         80         90        100
QCQQLDALLT RRRDGEPIAH LTGVREFWSL PLFVSPATLI PRPDTECLVE 
       110        120        130        140        150
QALARLPEQP CRILDLGTGT GAIALALASE RPDCEIIAVD RMPDAVSLAQ 
       160        170        180        190        200
RNAQHLAIKN IHILQSDWFS ALAGQQFAMI VSNPPYIDEQ DPHLQQGDVR 
       210        220        230        240        250
FEPLTALVAA DSGMADIVHI IEQSRNALVS GGFLLLEHGW QQGEAVRQAF 
       260        270 
ILAGYHDVET CRDYGDNERV TLGRYYQ
Length:277
Mass (Da):30,975
Last modified:November 8, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i73859DF7E983B9AA
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence BAA05915 differs from that shown. Reason: Frameshift.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
D28567 Genomic DNA Translation: BAA05915.1 Frameshift.
U18555 Genomic DNA Translation: AAC43438.1
AB078778 Genomic DNA Translation: BAB84109.1
U00096 Genomic DNA Translation: AAC74296.1
AP009048 Genomic DNA Translation: BAA36070.1

Protein sequence database of the Protein Information Resource

More...PIRi		I83570

NCBI Reference Sequences

More...RefSeqi		NP_415730.1, NC_000913.3
WP_000456467.1, NZ_SSZK01000010.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC74296; AAC74296; b1212
BAA36070; BAA36070; BAA36070

Database of genes from NCBI RefSeq genomes

More...GeneIDi		58389078
945779

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW1203
eco:b1212

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.1072

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28567 Genomic DNA Translation: BAA05915.1 Frameshift.
U18555 Genomic DNA Translation: AAC43438.1
AB078778 Genomic DNA Translation: BAB84109.1
U00096 Genomic DNA Translation: AAC74296.1
AP009048 Genomic DNA Translation: BAA36070.1
PIRiI83570
RefSeqiNP_415730.1, NC_000913.3
WP_000456467.1, NZ_SSZK01000010.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T43X-ray3.20A1-277[»]
2B3TX-ray3.10A1-276[»]
SMRiP0ACC1
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi4260113, 35 interactors
IntActiP0ACC1, 1 interactor
STRINGi511145.b1212

Chemistry databases

DrugBankiDB01752, S-adenosyl-L-homocysteine

Proteomic databases

PaxDbiP0ACC1
PRIDEiP0ACC1

Genome annotation databases

EnsemblBacteriaiAAC74296; AAC74296; b1212
BAA36070; BAA36070; BAA36070
GeneIDi58389078
945779
KEGGiecj:JW1203
eco:b1212
PATRICifig|1411691.4.peg.1072

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB2323

Phylogenomic databases

eggNOGiCOG2890, Bacteria
HOGENOMiCLU_018398_3_1_6
InParanoidiP0ACC1
PhylomeDBiP0ACC1

Enzyme and pathway databases

BioCyciEcoCyc:EG12424-MONOMER
MetaCyc:EG12424-MONOMER
BRENDAi2.1.1.297, 2026

Miscellaneous databases

EvolutionaryTraceiP0ACC1

Protein Ontology

More...PROi		PR:P0ACC1

Family and domain databases

HAMAPiMF_02126, RF_methyltr_PrmC, 1 hit
InterProiView protein in InterPro
IPR002052, DNA_methylase_N6_adenine_CS
IPR004556, HemK-like
IPR025714, Methyltranfer_dom
IPR040758, PrmC_N
IPR019874, Release_fac_Glu-N5_MeTfrase
IPR029063, SAM-dependent_MTases
PfamiView protein in Pfam
PF13847, Methyltransf_31, 1 hit
PF17827, PrmC_N, 1 hit
SUPFAMiSSF53335, SSF53335, 1 hit
TIGRFAMsiTIGR00536, hemK_fam, 1 hit
TIGR03534, RF_mod_PrmC, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPRMC_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0ACC1
Secondary accession number(s): P37186, Q46754
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: April 7, 2021
This is version 120 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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