UniProtKB - P0ACC1 (PRMC_ECOLI)
Protein
Release factor glutamine methyltransferase
Gene
prmC
Organism
Escherichia coli (strain K12)
Status
Functioni
Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif, i.e. on 'Gln-235' in RF1 and on 'Gln-252' in RF2.3 Publications
Caution
Was originally thought to be involved in the oxidation of protoporphyrinogen into protoporphyrin IX.1 Publication
Catalytic activityi
- L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine = H+ + N5-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine2 PublicationsEC:2.1.1.2972 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 140 | S-adenosyl-L-methionine2 Publications | 1 | |
Binding sitei | 168 | S-adenosyl-L-methionine2 Publications | 1 | |
Binding sitei | 183 | S-adenosyl-L-methionine1 Publication | 1 |
GO - Molecular functioni
- nucleic acid binding Source: InterPro
- protein-(glutamine-N5) methyltransferase activity Source: UniProtKB-EC
- protein-glutamine N-methyltransferase activity Source: UniProtKB
- protein methyltransferase activity Source: UniProtKB
- S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB
GO - Biological processi
- peptidyl-glutamine methylation Source: UniProtKB
- protein methylation Source: UniProtKB
- regulation of gene expression Source: UniProtKB
- translational termination Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12424-MONOMER MetaCyc:EG12424-MONOMER |
BRENDAi | 2.1.1.297, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Release factor glutamine methyltransferaseUniRule annotation (EC:2.1.1.2972 Publications)Short name: RF MTaseUniRule annotation Alternative name(s): M.EcoKHemKP N5-glutamine methyltransferase PrmCUniRule annotation Protein release factor methylation C Protein-(glutamine-N5) MTase PrmCUniRule annotation Protein-glutamine N-methyltransferase PrmCUniRule annotation |
Gene namesi | Name:prmC Synonyms:hemK Ordered Locus Names:b1212, JW1203 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene suffer severe growth defects, but also show a global shift in gene expression to anaerobic respiration. Also shows defects in translational termination via an enhanced rate of read-through of nonsense codons and induction of transfer-mRNA-mediated tagging of proteins within the cell.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 36 | R → A: 17% of wild-type RF1 methylation activity. 1 Publication | 1 | |
Mutagenesisi | 40 | L → A: 60% of wild-type RF1 methylation activity. 1 Publication | 1 | |
Mutagenesisi | 41 | A → R: 7% of wild-type RF1 methylation activity. Strongly reduced affinity for S-adenosyl-L-methionine. 1 Publication | 1 | |
Mutagenesisi | 42 | F → A: 40% of wild-type RF1 methylation activity. 1 Publication | 1 | |
Mutagenesisi | 44 | E → A: 62% of wild-type RF1 methylation activity. 1 Publication | 1 | |
Mutagenesisi | 44 | E → R: 33% of wild-type RF1 methylation activity. 1 Publication | 1 | |
Mutagenesisi | 183 | N → A: Less than 2% of wild-type RF1 methylation activity. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB01752, S-adenosyl-L-homocysteine |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000157156 | 1 – 277 | Release factor glutamine methyltransferaseAdd BLAST | 277 |
Proteomic databases
PaxDbi | P0ACC1 |
PRIDEi | P0ACC1 |
Interactioni
Subunit structurei
Interacts with PrfA and PrfB.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4260113, 35 interactors |
IntActi | P0ACC1, 1 interactor |
STRINGi | 511145.b1212 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0ACC1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ACC1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 117 – 121 | S-adenosyl-L-methionine bindingUniRule annotation1 Publication | 5 | |
Regioni | 183 – 186 | Substrate binding1 Publication | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2890, Bacteria |
HOGENOMi | CLU_018398_3_1_6 |
InParanoidi | P0ACC1 |
PhylomeDBi | P0ACC1 |
Family and domain databases
HAMAPi | MF_02126, RF_methyltr_PrmC, 1 hit |
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR004556, HemK-like IPR025714, Methyltranfer_dom IPR040758, PrmC_N IPR019874, Release_fac_Glu-N5_MeTfrase IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF13847, Methyltransf_31, 1 hit PF17827, PrmC_N, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00536, hemK_fam, 1 hit TIGR03534, RF_mod_PrmC, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ACC1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEYQHWLREA ISQLQASESP RRDAEILLEH VTGRGRTFIL AFGETQLTDE
60 70 80 90 100
QCQQLDALLT RRRDGEPIAH LTGVREFWSL PLFVSPATLI PRPDTECLVE
110 120 130 140 150
QALARLPEQP CRILDLGTGT GAIALALASE RPDCEIIAVD RMPDAVSLAQ
160 170 180 190 200
RNAQHLAIKN IHILQSDWFS ALAGQQFAMI VSNPPYIDEQ DPHLQQGDVR
210 220 230 240 250
FEPLTALVAA DSGMADIVHI IEQSRNALVS GGFLLLEHGW QQGEAVRQAF
260 270
ILAGYHDVET CRDYGDNERV TLGRYYQ
Sequence cautioni
The sequence BAA05915 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D28567 Genomic DNA Translation: BAA05915.1 Frameshift. U18555 Genomic DNA Translation: AAC43438.1 AB078778 Genomic DNA Translation: BAB84109.1 U00096 Genomic DNA Translation: AAC74296.1 AP009048 Genomic DNA Translation: BAA36070.1 |
PIRi | I83570 |
RefSeqi | NP_415730.1, NC_000913.3 WP_000456467.1, NZ_SSZK01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC74296; AAC74296; b1212 BAA36070; BAA36070; BAA36070 |
GeneIDi | 48106281 945779 |
KEGGi | ecj:JW1203 eco:b1212 |
PATRICi | fig|1411691.4.peg.1072 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D28567 Genomic DNA Translation: BAA05915.1 Frameshift. U18555 Genomic DNA Translation: AAC43438.1 AB078778 Genomic DNA Translation: BAB84109.1 U00096 Genomic DNA Translation: AAC74296.1 AP009048 Genomic DNA Translation: BAA36070.1 |
PIRi | I83570 |
RefSeqi | NP_415730.1, NC_000913.3 WP_000456467.1, NZ_SSZK01000010.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1T43 | X-ray | 3.20 | A | 1-277 | [»] | |
2B3T | X-ray | 3.10 | A | 1-276 | [»] | |
SMRi | P0ACC1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260113, 35 interactors |
IntActi | P0ACC1, 1 interactor |
STRINGi | 511145.b1212 |
Chemistry databases
DrugBanki | DB01752, S-adenosyl-L-homocysteine |
Proteomic databases
PaxDbi | P0ACC1 |
PRIDEi | P0ACC1 |
Genome annotation databases
EnsemblBacteriai | AAC74296; AAC74296; b1212 BAA36070; BAA36070; BAA36070 |
GeneIDi | 48106281 945779 |
KEGGi | ecj:JW1203 eco:b1212 |
PATRICi | fig|1411691.4.peg.1072 |
Organism-specific databases
EchoBASEi | EB2323 |
Phylogenomic databases
eggNOGi | COG2890, Bacteria |
HOGENOMi | CLU_018398_3_1_6 |
InParanoidi | P0ACC1 |
PhylomeDBi | P0ACC1 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12424-MONOMER MetaCyc:EG12424-MONOMER |
BRENDAi | 2.1.1.297, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0ACC1 |
PROi | PR:P0ACC1 |
Family and domain databases
HAMAPi | MF_02126, RF_methyltr_PrmC, 1 hit |
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR004556, HemK-like IPR025714, Methyltranfer_dom IPR040758, PrmC_N IPR019874, Release_fac_Glu-N5_MeTfrase IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF13847, Methyltransf_31, 1 hit PF17827, PrmC_N, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00536, hemK_fam, 1 hit TIGR03534, RF_mod_PrmC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRMC_ECOLI | |
Accessioni | P0ACC1Primary (citable) accession number: P0ACC1 Secondary accession number(s): P37186, Q46754 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | December 2, 2020 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families