hemG

Functionⁱ

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX using menaquinone as electron acceptor.

Catalytic activityⁱ

Protoporphyrinogen IX + 3 menaquinone = protoporphyrin IX + 3 menaquinol.

Pathwayⁱ: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.
Proteins known to be involved in this subpathway in this organism are:

Protoporphyrinogen IX dehydrogenase [menaquinone] (hemG)

This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functionⁱ

electron transfer activity Source: InterPro
FMN binding
Source: EcoCyc
Inferred from direct assayⁱ
- 19583219
menaquinone-dependent protoporphyrinogen oxidase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 19583219
oxygen-dependent protoporphyrinogen oxidase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 7916647

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

heme biosynthetic process
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 390093
porphyrin-containing compound biosynthetic process
Source: EcoliWiki
Inferred from mutant phenotypeⁱ
- 7788523
protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Oxidoreductase
Biological process	Porphyrin biosynthesis

Enzyme and pathway databases

BioCycⁱ	EcoCyc:PROTOPORGENOXI-MONOMER MetaCyc:PROTOPORGENOXI-MONOMER
UniPathwayⁱ	UPA00251;UER00324

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Protoporphyrinogen IX dehydrogenase [menaquinone] (EC:1.3.5.3)
Gene namesⁱ	Name:hemG Synonyms:yihB Ordered Locus Names:b3850, JW3827
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11485 hemG

EcoGeneⁱ

EG11485 hemG

Subcellular locationⁱ

GO - Cellular componentⁱ

cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 17309111

View the complete GO annotation on QuickGO ...

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000135259	1 – 181	Protoporphyrinogen IX dehydrogenase [menaquinone]Add BLAST		181

Proteomic databases

PaxDbⁱ	P0ACB4
PRIDEⁱ	P0ACB4

Interactionⁱ

Subunit structureⁱ

Belongs to a multi-protein complex.

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
frdA	P00363	2	EBI-1115706,EBI-550480

With

Entry

#Exp.

IntAct

Notes

frdA

P00363

2

EBI-1115706,EBI-550480

Protein-protein interaction databases

BioGridⁱ	4262620, 2 interactors
Database of interacting proteins More...DIPⁱ	DIP-35890N
IntActⁱ	P0ACB4, 19 interactors
STRINGⁱ	316385.ECDH10B_4039

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P0ACB4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	3 – 172	Flavodoxin-likePROSITE-ProRule annotationAdd BLAST		170

Phylogenomic databases

eggNOGⁱ	ENOG4108RR8 Bacteria COG4635 LUCA
HOGENOMⁱ	HOG000251295
InParanoidⁱ	P0ACB4
KEGG Orthology (KO) More...KOⁱ	K00230
PhylomeDBⁱ	P0ACB4

Family and domain databases

Gene3Dⁱ	3.40.50.360, 1 hit
InterProⁱ	View protein in InterPro IPR008254 Flavodoxin/NO_synth IPR001226 Flavodoxin_CS IPR026816 Flavodoxin_dom IPR029039 Flavoprotein-like_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF12724 Flavodoxin_5, 1 hit
SUPFAMⁱ	SSF52218 SSF52218, 1 hit
PROSITEⁱ	View protein in PROSITE PS00201 FLAVODOXIN, 1 hit PS50902 FLAVODOXIN_LIKE, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0ACB4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKTLILFSTR DGQTREIASY LASELKELGI QADVANVHRI EEPQWENYDR 
        60         70         80         90        100
VVIGASIRYG HYHSAFQEFV KKHATRLNSM PSAFYSVNLV ARKPEKRTPQ 
       110        120        130        140        150
TNSYARKFLM NSQWRPDRCA VIAGALRYPR YRWYDRFMIK LIMKMSGGET 
       160        170        180 
DTRKEVVYTD WEQVANFARE IAHLTDKPTL K

Length:181

Mass (Da):21,226

Last modified:November 8, 2005 - v1

Checksum:ⁱBCCB5AE594010026

GO

Sequence cautionⁱ

The sequence X54687 differs from that shown. Reason: Frameshift at position 159.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X54687 Genomic DNA No translation available. X68660 Genomic DNA Translation: CAA48626.1 M87049 Genomic DNA Translation: AAA67647.1 U00096 Genomic DNA Translation: AAC76853.1 AP009048 Genomic DNA Translation: BAE77453.1
PIRⁱ	JC2513
NCBI Reference Sequences More...RefSeqⁱ	NP_418292.1, NC_000913.3 WP_000853959.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC76853; AAC76853; b3850 BAE77453; BAE77453; BAE77453
GeneIDⁱ	948331
KEGGⁱ	ecj:JW3827 eco:b3850
PATRICⁱ	fig\|1411691.4.peg.2860

Protein	Similar proteins		Species	Score	Length	Source
P0ACB4	Protoporphyrinogen IX dehydrogenase [menaquinone]		ECO57		181	UniRef100_P0ACB6
Protoporphyrinogen IX dehydrogenase [menaquinone]		ECOL6		181
Protoporphyrinogen oxidase		Shigella sp.		181
Protoporphyrinogen oxidase		Escherichia coli O145 str. RM9872		181
Protoporphyrinogen IX oxidase,oxygen-independent, HemG		Klebsiella pneumoniae IS22		181
+93

Protein	Similar proteins		Species	Score	Length	Source
P0ACB4	Protoporphyrinogen IX dehydrogenase [menaquinone]		ECO57		181	UniRef90_P0ACB6
Protoporphyrinogen IX dehydrogenase [menaquinone]		ECOL6		181
Protoporphyrinogen oxidase		Shigella sp.		181
Protoporphyrinogen oxidase		Escherichia coli O145 str. RM9872		181
Protoporphyrinogen IX oxidase,oxygen-independent, HemG		Klebsiella pneumoniae IS22		181
+384

Protein	Similar proteins		Species	Score	Length	Source
P0ACB4	Protoporphyrinogen IX dehydrogenase [menaquinone]		SALTY		181	UniRef50_Q9L6L1
Protoporphyrinogen IX dehydrogenase [menaquinone]		ECO57		181
Protoporphyrinogen IX dehydrogenase [menaquinone]		ECOL6		181
Flavodoxin/nitric oxide synthase		SALET		181
Protoporphyrinogen oxidase		SALCE		181
+1399

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X54687 Genomic DNA No translation available. X68660 Genomic DNA Translation: CAA48626.1 M87049 Genomic DNA Translation: AAA67647.1 U00096 Genomic DNA Translation: AAC76853.1 AP009048 Genomic DNA Translation: BAE77453.1
PIRⁱ	JC2513
RefSeqⁱ	NP_418292.1, NC_000913.3 WP_000853959.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P0ACB4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4262620, 2 interactors
DIPⁱ	DIP-35890N
IntActⁱ	P0ACB4, 19 interactors
STRINGⁱ	316385.ECDH10B_4039

Proteomic databases

PaxDbⁱ	P0ACB4
PRIDEⁱ	P0ACB4

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76853; AAC76853; b3850 BAE77453; BAE77453; BAE77453
GeneIDⁱ	948331
KEGGⁱ	ecj:JW3827 eco:b3850
PATRICⁱ	fig\|1411691.4.peg.2860

Organism-specific databases

EchoBASEⁱ	EB1448
EcoGeneⁱ	EG11485 hemG

Phylogenomic databases

eggNOGⁱ	ENOG4108RR8 Bacteria COG4635 LUCA
HOGENOMⁱ	HOG000251295
InParanoidⁱ	P0ACB4
KOⁱ	K00230
PhylomeDBⁱ	P0ACB4

Enzyme and pathway databases

UniPathwayⁱ	UPA00251;UER00324
BioCycⁱ	EcoCyc:PROTOPORGENOXI-MONOMER MetaCyc:PROTOPORGENOXI-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P0ACB4

PROⁱ

PR:P0ACB4

Family and domain databases

Gene3Dⁱ	3.40.50.360, 1 hit
InterProⁱ	View protein in InterPro IPR008254 Flavodoxin/NO_synth IPR001226 Flavodoxin_CS IPR026816 Flavodoxin_dom IPR029039 Flavoprotein-like_sf
Pfamⁱ	View protein in Pfam PF12724 Flavodoxin_5, 1 hit
SUPFAMⁱ	SSF52218 SSF52218, 1 hit
PROSITEⁱ	View protein in PROSITE PS00201 FLAVODOXIN, 1 hit PS50902 FLAVODOXIN_LIKE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	HEMG_ECOLI
Accessionⁱ	P0ACB4Primary (citable) accession number: P0ACB4 Secondary accession number(s): P27863, Q2M8F3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
	Last sequence update:	November 8, 2005
	Last modified:	November 7, 2018
	This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

PATHWAY comments
Index of metabolic and biosynthesis pathways
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0ACB4 (HEMG_ECOLI)

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Protoporphyrinogen IX dehydrogenase [menaquinone]

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protoporphyrin-IX biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Pathwayⁱ: protoporphyrin-IX biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ