UniProtKB - P0ACB4 (HEMG_ECOLI)
Protoporphyrinogen IX dehydrogenase [quinone]
hemG
Functioni
Catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX; under anaerobic conditions uses menaquinone as an electron acceptor, under aerobic condition uses ubiquinone as an electron acceptor.
UniRule annotationAnaerobically in vitro transfers electrons to fumarate reductase and nitrate reductase; transfer to nitrate reductase couples this reaction to electron transfer across the cell inner membrane and thus ATP synthesis (PubMed:19583219, PubMed:20484676).
Neither mesoporphyrinogen nor coproporphyrinogen are substrates (PubMed:19583219).
Under aerobic conditions in vitro forms protoporphyrin IX using ubiquinone as an electron acceptor, is able to transfer electrons to cytochrome bd oxidase and cytochrome bo oxidase; transfer to these oxidases couples this reaction to electron transfer across the cell inner membrane and thus ATP synthesis. In cell free extracts deletion of both cytochrome oxidases prevents formation of protoporphyrin IX (PubMed:20484676).
2 PublicationsCatalytic activityi
- 3 a menaquinone + protoporphyrinogen IX = 3 a menaquinol + protoporphyrin IXUniRule annotation2 PublicationsEC:1.3.5.3UniRule annotation2 Publications
- 3 a ubiquinone + protoporphyrinogen IX = 3 a ubiquinol + protoporphyrin IXUniRule annotation1 Publication
- EC:1.3.5.3UniRule annotation1 Publication
Cofactori
Activity regulationi
Redox potential
Kineticsi
- KM=7.0 µM for protoporphyrinogen IX1 Publication
- KM=3.76 µM for menadione (a soluble menaquinone analog)1 Publication
- KM=17.3 µM for menaquinone1 Publication
- Vmax=960 µmol/h/mg enzyme1 Publication
: protoporphyrin-IX biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.UniRule annotation2 Publications This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 9 – 13 | FMNUniRule annotation | 5 | |
Nucleotide bindingi | 84 – 152 | FMNUniRule annotationAdd BLAST | 69 |
GO - Molecular functioni
- electron transfer activity Source: InterPro
- FMN binding Source: EcoCyc
- menaquinone-dependent protoporphyrinogen oxidase activity Source: EcoCyc
- oxygen-dependent protoporphyrinogen oxidase activity Source: EcoCyc
GO - Biological processi
- heme biosynthetic process Source: EcoCyc
- porphyrin-containing compound biosynthetic process Source: EcoliWiki
- protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Porphyrin biosynthesis |
Ligand | Flavoprotein, FMN, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:PROTOPORGENOXI-MONOMER |
SABIO-RKi | P0ACB4 |
UniPathwayi | UPA00251;UER00324 |
Names & Taxonomyi
Protein namesi | Recommended name: Protoporphyrinogen IX dehydrogenase [quinone]UniRule annotation (EC:1.3.5.3UniRule annotation2 Publications)Alternative name(s): Protoporphyrinogen IX dehydrogenase [menaquinone]UniRule annotation Protoporphyrinogen IX dehydrogenase [ubiquinone]UniRule annotation Protoporphyrinogen oxidase1 PublicationUniRule annotation Short name: PPO1 PublicationUniRule annotation |
Gene namesi | Name:hemG1 PublicationUniRule annotation Synonyms:yihB Ordered Locus Names:b3850, JW3827 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation1 Publication; Peripheral membrane protein UniRule annotation1 Publication
Cytosol
- cytosol Source: EcoCyc
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000135259 | 1 – 181 | Protoporphyrinogen IX dehydrogenase [quinone]Add BLAST | 181 |
Proteomic databases
jPOSTi | P0ACB4 |
PaxDbi | P0ACB4 |
PRIDEi | P0ACB4 |
Interactioni
Subunit structurei
Homotetramer (Probable). Homohexamer. Purified from membrane fractions associated with fumarate reductase (subunit FrdA) (PubMed:20484676).
1 Publication1 PublicationBinary interactionsi
P0ACB4
With | #Exp. | IntAct |
---|---|---|
frdA [P00363] | 2 | EBI-1115706,EBI-550480 |
Protein-protein interaction databases
BioGRIDi | 4262620, 2 interactors |
DIPi | DIP-35890N |
IntActi | P0ACB4, 19 interactors |
STRINGi | 511145.b3850 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 172 | Flavodoxin-likeUniRule annotationAdd BLAST | 170 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG4635, Bacteria |
HOGENOMi | CLU_094839_0_1_6 |
InParanoidi | P0ACB4 |
PhylomeDBi | P0ACB4 |
Family and domain databases
Gene3Di | 3.40.50.360, 1 hit |
HAMAPi | MF_00853, HemG, 1 hit |
InterProi | View protein in InterPro IPR008254, Flavodoxin/NO_synth IPR001226, Flavodoxin_CS IPR026816, Flavodoxin_dom IPR029039, Flavoprotein-like_sf IPR044264, HemG |
Pfami | View protein in Pfam PF12724, Flavodoxin_5, 1 hit |
SUPFAMi | SSF52218, SSF52218, 1 hit |
PROSITEi | View protein in PROSITE PS00201, FLAVODOXIN, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKTLILFSTR DGQTREIASY LASELKELGI QADVANVHRI EEPQWENYDR
60 70 80 90 100
VVIGASIRYG HYHSAFQEFV KKHATRLNSM PSAFYSVNLV ARKPEKRTPQ
110 120 130 140 150
TNSYARKFLM NSQWRPDRCA VIAGALRYPR YRWYDRFMIK LIMKMSGGET
160 170 180
DTRKEVVYTD WEQVANFARE IAHLTDKPTL K
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X54687 Genomic DNA No translation available. X68660 Genomic DNA Translation: CAA48626.1 M87049 Genomic DNA Translation: AAA67647.1 U00096 Genomic DNA Translation: AAC76853.1 AP009048 Genomic DNA Translation: BAE77453.1 |
PIRi | JC2513 |
RefSeqi | NP_418292.1, NC_000913.3 WP_000853959.1, NZ_STEB01000021.1 |
Genome annotation databases
EnsemblBacteriai | AAC76853; AAC76853; b3850 BAE77453; BAE77453; BAE77453 |
GeneIDi | 58462188 948331 |
KEGGi | ecj:JW3827 eco:b3850 |
PATRICi | fig|1411691.4.peg.2860 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X54687 Genomic DNA No translation available. X68660 Genomic DNA Translation: CAA48626.1 M87049 Genomic DNA Translation: AAA67647.1 U00096 Genomic DNA Translation: AAC76853.1 AP009048 Genomic DNA Translation: BAE77453.1 |
PIRi | JC2513 |
RefSeqi | NP_418292.1, NC_000913.3 WP_000853959.1, NZ_STEB01000021.1 |
3D structure databases
SMRi | P0ACB4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262620, 2 interactors |
DIPi | DIP-35890N |
IntActi | P0ACB4, 19 interactors |
STRINGi | 511145.b3850 |
Proteomic databases
jPOSTi | P0ACB4 |
PaxDbi | P0ACB4 |
PRIDEi | P0ACB4 |
Genome annotation databases
EnsemblBacteriai | AAC76853; AAC76853; b3850 BAE77453; BAE77453; BAE77453 |
GeneIDi | 58462188 948331 |
KEGGi | ecj:JW3827 eco:b3850 |
PATRICi | fig|1411691.4.peg.2860 |
Organism-specific databases
EchoBASEi | EB1448 |
Phylogenomic databases
eggNOGi | COG4635, Bacteria |
HOGENOMi | CLU_094839_0_1_6 |
InParanoidi | P0ACB4 |
PhylomeDBi | P0ACB4 |
Enzyme and pathway databases
UniPathwayi | UPA00251;UER00324 |
BioCyci | EcoCyc:PROTOPORGENOXI-MONOMER |
SABIO-RKi | P0ACB4 |
Miscellaneous databases
PROi | PR:P0ACB4 |
Family and domain databases
Gene3Di | 3.40.50.360, 1 hit |
HAMAPi | MF_00853, HemG, 1 hit |
InterProi | View protein in InterPro IPR008254, Flavodoxin/NO_synth IPR001226, Flavodoxin_CS IPR026816, Flavodoxin_dom IPR029039, Flavoprotein-like_sf IPR044264, HemG |
Pfami | View protein in Pfam PF12724, Flavodoxin_5, 1 hit |
SUPFAMi | SSF52218, SSF52218, 1 hit |
PROSITEi | View protein in PROSITE PS00201, FLAVODOXIN, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HEMG_ECOLI | |
Accessioni | P0ACB4Primary (citable) accession number: P0ACB4 Secondary accession number(s): P27863, Q2M8F3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | February 23, 2022 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families