We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P0ACB2 (HEM2_ECOLI)
Protein
Delta-aminolevulinic acid dehydratase
Gene
hemB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.
Catalytic activityi
- EC:4.2.1.241 Publication
Cofactori
Zn2+5 PublicationsNote: Binds 1 zinc ion per monomer.4 Publications
Activity regulationi
Allosteric enzyme. Stimulated by magnesium ions.1 Publication
Kineticsi
- KM=800 µM for 5-aminolevulinic acid1 Publication
pH dependencei
Optimum pH is 8.5.1 Publication
: protoporphyrin-IX biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate. This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 120 | Zinc; catalytic2 Publications | 1 | |
Metal bindingi | 122 | Zinc; catalytic2 Publications | 1 | |
Metal bindingi | 130 | Zinc; catalytic2 Publications | 1 | |
Active sitei | 195 | Schiff-base intermediate with substrate | 1 | |
Binding sitei | 205 | Substrate 1 | 1 | |
Binding sitei | 216 | Substrate 1 | 1 | |
Metal bindingi | 232 | Magnesium2 Publications | 1 | |
Active sitei | 247 | Schiff-base intermediate with substrate | 1 | |
Binding sitei | 273 | Substrate 2 | 1 | |
Binding sitei | 312 | Substrate 2 | 1 |
GO - Molecular functioni
- magnesium ion binding Source: EcoCyc
- porphobilinogen synthase activity Source: EcoCyc
- zinc ion binding Source: EcoCyc
GO - Biological processi
- heme biosynthetic process Source: EcoliWiki
- protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Allosteric enzyme, Lyase |
Biological process | Heme biosynthesis, Porphyrin biosynthesis |
Ligand | Magnesium, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:PORPHOBILSYNTH-MONOMER |
BRENDAi | 4.2.1.24, 2026 |
UniPathwayi | UPA00251;UER00318 |
Names & Taxonomyi
Protein namesi | Recommended name: Delta-aminolevulinic acid dehydratase (EC:4.2.1.241 Publication)Short name: ALAD Short name: ALADH Alternative name(s): Porphobilinogen synthase |
Gene namesi | Name:hemB Synonyms:ncf Ordered Locus Names:b0369, JW0361 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 127 | H → A: No significant effect on activity; when associated with A-129. 1 Publication | 1 | |
Mutagenesisi | 129 | H → A: No significant effect on activity; when associated with A-127. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL3286083 |
DrugBanki | DB04560, 4,7-Dioxosebacic Acid DB02260, 4-Oxosebacic Acid DB04530, S,S-(2-Hydroxyethyl)Thiocysteine |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000140501 | 2 – 324 | Delta-aminolevulinic acid dehydrataseAdd BLAST | 323 |
Proteomic databases
jPOSTi | P0ACB2 |
PaxDbi | P0ACB2 |
PRIDEi | P0ACB2 |
2D gel databases
SWISS-2DPAGEi | P0ACB2 |
Interactioni
Subunit structurei
Homooctamer.
4 PublicationsProtein-protein interaction databases
BioGRIDi | 4261502, 26 interactors |
DIPi | DIP-36209N |
IntActi | P0ACB2, 8 interactors |
MINTi | P0ACB2 |
STRINGi | 511145.b0369 |
Chemistry databases
BindingDBi | P0ACB2 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0ACB2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ACB2 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ALAD family.Curated
Phylogenomic databases
eggNOGi | COG0113, Bacteria |
HOGENOMi | CLU_035731_0_0_6 |
InParanoidi | P0ACB2 |
PhylomeDBi | P0ACB2 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR001731, ALAD IPR030656, ALAD_AS IPR013785, Aldolase_TIM |
PANTHERi | PTHR11458, PTHR11458, 1 hit |
Pfami | View protein in Pfam PF00490, ALAD, 1 hit |
PIRSFi | PIRSF001415, Porphbilin_synth, 1 hit |
PRINTSi | PR00144, DALDHYDRTASE |
SMARTi | View protein in SMART SM01004, ALAD, 1 hit |
PROSITEi | View protein in PROSITE PS00169, D_ALA_DEHYDRATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0ACB2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDLIQRPRR LRKSPALRAM FEETTLSLND LVLPIFVEEE IDDYKAVEAM
60 70 80 90 100
PGVMRIPEKH LAREIERIAN AGIRSVMTFG ISHHTDETGS DAWREDGLVA
110 120 130 140 150
RMSRICKQTV PEMIVMSDTC FCEYTSHGHC GVLCEHGVDN DATLENLGKQ
160 170 180 190 200
AVVAAAAGAD FIAPSAAMDG QVQAIRQALD AAGFKDTAIM SYSTKFASSF
210 220 230 240 250
YGPFREAAGS ALKGDRKSYQ MNPMNRREAI RESLLDEAQG ADCLMVKPAG
260 270 280 290 300
AYLDIVRELR ERTELPIGAY QVSGEYAMIK FAALAGAIDE EKVVLESLGS
310 320
IKRAGADLIF SYFALDLAEK KILR
Sequence cautioni
The sequence AAB18092 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence BAA12842 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 18 – 19 | RA → PR in AAA62629 (PubMed:2656410).Curated | 2 | |
Sequence conflicti | 19 – 42 | AMFEE…EEEID → VCLKRQHLSLTTWCCRSLLK KKLT in CAA35467 (PubMed:2464127).CuratedAdd BLAST | 24 | |
Sequence conflicti | 90 – 91 | SD → ER in AAA62629 (PubMed:2656410).Curated | 2 | |
Sequence conflicti | 104 | R → P in AAA62629 (PubMed:2656410).Curated | 1 | |
Sequence conflicti | 226 | R → P in CAA35467 (PubMed:2464127).Curated | 1 | |
Sequence conflicti | 227 | R → A in AAA62629 (PubMed:2656410).Curated | 1 | |
Sequence conflicti | 229 | A → G in AAA62629 (PubMed:2656410).Curated | 1 | |
Sequence conflicti | 231 | R → A in AAA62629 (PubMed:2656410).Curated | 1 | |
Sequence conflicti | 233 | S → Y in AAA62629 (PubMed:2656410).Curated | 1 | |
Sequence conflicti | 241 | A → P in AAA62629 (PubMed:2656410).Curated | 1 | |
Sequence conflicti | 254 | D → N in AAA62629 (PubMed:2656410).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24488 Genomic DNA Translation: AAA62629.1 X17417 Genomic DNA Translation: CAA35467.1 L44595 Genomic DNA Translation: AAB52499.1 D85613 Genomic DNA Translation: BAA12842.1 Different initiation. U73857 Genomic DNA Translation: AAB18092.1 Different initiation. U00096 Genomic DNA Translation: AAC73472.2 AP009048 Genomic DNA Translation: BAE76150.1 |
PIRi | A64765, SYECPF |
RefSeqi | NP_414903.4, NC_000913.3 WP_001295337.1, NZ_STEB01000036.1 |
Genome annotation databases
EnsemblBacteriai | AAC73472; AAC73472; b0369 BAE76150; BAE76150; BAE76150 |
GeneIDi | 66671329 945017 |
KEGGi | ecj:JW0361 eco:b0369 |
PATRICi | fig|1411691.4.peg.1910 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24488 Genomic DNA Translation: AAA62629.1 X17417 Genomic DNA Translation: CAA35467.1 L44595 Genomic DNA Translation: AAB52499.1 D85613 Genomic DNA Translation: BAA12842.1 Different initiation. U73857 Genomic DNA Translation: AAB18092.1 Different initiation. U00096 Genomic DNA Translation: AAC73472.2 AP009048 Genomic DNA Translation: BAE76150.1 |
PIRi | A64765, SYECPF |
RefSeqi | NP_414903.4, NC_000913.3 WP_001295337.1, NZ_STEB01000036.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1B4E | X-ray | 2.00 | A | 2-324 | [»] | |
1I8J | X-ray | 1.90 | A/B | 2-324 | [»] | |
1L6S | X-ray | 1.70 | A/B | 2-324 | [»] | |
1L6Y | X-ray | 1.90 | A/B | 2-324 | [»] | |
5MHB | X-ray | 2.10 | A | 1-324 | [»] | |
SMRi | P0ACB2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261502, 26 interactors |
DIPi | DIP-36209N |
IntActi | P0ACB2, 8 interactors |
MINTi | P0ACB2 |
STRINGi | 511145.b0369 |
Chemistry databases
BindingDBi | P0ACB2 |
ChEMBLi | CHEMBL3286083 |
DrugBanki | DB04560, 4,7-Dioxosebacic Acid DB02260, 4-Oxosebacic Acid DB04530, S,S-(2-Hydroxyethyl)Thiocysteine |
2D gel databases
SWISS-2DPAGEi | P0ACB2 |
Proteomic databases
jPOSTi | P0ACB2 |
PaxDbi | P0ACB2 |
PRIDEi | P0ACB2 |
Genome annotation databases
EnsemblBacteriai | AAC73472; AAC73472; b0369 BAE76150; BAE76150; BAE76150 |
GeneIDi | 66671329 945017 |
KEGGi | ecj:JW0361 eco:b0369 |
PATRICi | fig|1411691.4.peg.1910 |
Organism-specific databases
EchoBASEi | EB0423 |
Phylogenomic databases
eggNOGi | COG0113, Bacteria |
HOGENOMi | CLU_035731_0_0_6 |
InParanoidi | P0ACB2 |
PhylomeDBi | P0ACB2 |
Enzyme and pathway databases
UniPathwayi | UPA00251;UER00318 |
BioCyci | EcoCyc:PORPHOBILSYNTH-MONOMER |
BRENDAi | 4.2.1.24, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0ACB2 |
PROi | PR:P0ACB2 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR001731, ALAD IPR030656, ALAD_AS IPR013785, Aldolase_TIM |
PANTHERi | PTHR11458, PTHR11458, 1 hit |
Pfami | View protein in Pfam PF00490, ALAD, 1 hit |
PIRSFi | PIRSF001415, Porphbilin_synth, 1 hit |
PRINTSi | PR00144, DALDHYDRTASE |
SMARTi | View protein in SMART SM01004, ALAD, 1 hit |
PROSITEi | View protein in PROSITE PS00169, D_ALA_DEHYDRATASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HEM2_ECOLI | |
Accessioni | P0ACB2Primary (citable) accession number: P0ACB2 Secondary accession number(s): P15002, P78247, Q2MC56 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 127 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families