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Protein

Replicative DNA helicase

Gene

dnaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi231 – 238ATPCurated8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • DNA helicase activity Source: EcoCyc
  • helicase activity Source: EcoCyc
  • identical protein binding Source: IntAct

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10236-MONOMER
MetaCyc:EG10236-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Replicative DNA helicase (EC:3.6.4.12)
Gene namesi
Name:dnaB
Synonyms:groP, grpA
Ordered Locus Names:b4052, JW4012
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10236 dnaB

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001020191 – 471Replicative DNA helicaseAdd BLAST471

Proteomic databases

EPDiP0ACB0
PaxDbiP0ACB0
PRIDEiP0ACB0

2D gel databases

SWISS-2DPAGEiP0ACB0

Expressioni

Inductioni

Slightly induced by hydroxyurea.1 Publication

Interactioni

Subunit structurei

Homohexamer.

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4261652, 171 interactors
ComplexPortaliCPX-1933 DnaB-DnaG complex
CPX-1934 DnaB-DnaC complex
CPX-1935 DnaA-DnaB complex
CPX-1946 DnaB helicase complex
CPX-1950 DnaA-DnaB-DnaC complex
CPX-1951 DnaB-DnaC-DnaT-PriA-PriB complex
CPX-1952 DnaB-DnaC-DnaT-PriA-PriC complex
CPX-1953 DnaB-DnaC-Rep-PriC complex
DIPiDIP-9456N
IntActiP0ACB0, 39 interactors
MINTiP0ACB0
STRINGi316385.ECDH10B_4241

Structurei

Secondary structure

1471
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0ACB0
SMRiP0ACB0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ACB0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini200 – 467SF4 helicasePROSITE-ProRule annotationAdd BLAST268

Sequence similaritiesi

Belongs to the helicase family. DnaB subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CDU Bacteria
COG0305 LUCA
HOGENOMiHOG000113196
InParanoidiP0ACB0
KOiK02314
PhylomeDBiP0ACB0

Family and domain databases

CDDicd00984 DnaB_C, 1 hit
Gene3Di1.10.860.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR036185 DNA_heli_DnaB-like_N_sf
IPR007692 DNA_helicase_DnaB
IPR007694 DNA_helicase_DnaB-like_C
IPR007693 DNA_helicase_DnaB-like_N
IPR016136 DNA_helicase_N/primase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00772 DnaB, 1 hit
PF03796 DnaB_C, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF48024 SSF48024, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00665 DnaB, 1 hit
PROSITEiView protein in PROSITE
PS51199 SF4_HELICASE, 1 hit

Sequencei

Sequence statusi: Complete.

P0ACB0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGNKPFNKQ QAEPRERDPQ VAGLKVPPHS IEAEQSVLGG LMLDNERWDD
60 70 80 90 100
VAERVVADDF YTRPHRHIFT EMARLQESGS PIDLITLAES LERQGQLDSV
110 120 130 140 150
GGFAYLAELS KNTPSAANIS AYADIVRERA VVREMISVAN EIAEAGFDPQ
160 170 180 190 200
GRTSEDLLDL AESRVFKIAE SRANKDEGPK NIADVLDATV ARIEQLFQQP
210 220 230 240 250
HDGVTGVNTG YDDLNKKTAG LQPSDLIIVA ARPSMGKTTF AMNLVENAAM
260 270 280 290 300
LQDKPVLIFS LEMPSEQIMM RSLASLSRVD QTKIRTGQLD DEDWARISGT
310 320 330 340 350
MGILLEKRNI YIDDSSGLTP TEVRSRARRI AREHGGIGLI MIDYLQLMRV
360 370 380 390 400
PALSDNRTLE IAEISRSLKA LAKELNVPVV ALSQLNRSLE QRADKRPVNS
410 420 430 440 450
DLRESGSIEQ DADLIMFIYR DEVYHENSDL KGIAEIIIGK QRNGPIGTVR
460 470
LTFNGQWSRF DNYAGPQYDD E
Length:471
Mass (Da):52,390
Last modified:August 13, 1987 - v1
Checksum:i5B5ED3625A7F7DE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01174 Genomic DNA Translation: AAA23689.1
L02312 Genomic DNA Translation: AAA23690.1
U00006 Genomic DNA Translation: AAC43146.1
U00096 Genomic DNA Translation: AAC77022.1
AP009048 Genomic DNA Translation: BAE78054.1
PIRiC65213 IQECDB
RefSeqiNP_418476.1, NC_000913.3
WP_000918363.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77022; AAC77022; b4052
BAE78054; BAE78054; BAE78054
GeneIDi948555
KEGGiecj:JW4012
eco:b4052
PATRICifig|1411691.4.peg.2655

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01174 Genomic DNA Translation: AAA23689.1
L02312 Genomic DNA Translation: AAA23690.1
U00006 Genomic DNA Translation: AAC43146.1
U00096 Genomic DNA Translation: AAC77022.1
AP009048 Genomic DNA Translation: BAE78054.1
PIRiC65213 IQECDB
RefSeqiNP_418476.1, NC_000913.3
WP_000918363.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B79X-ray2.30A/B/C/D16-129[»]
1JWENMR-A24-137[»]
ProteinModelPortaliP0ACB0
SMRiP0ACB0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261652, 171 interactors
ComplexPortaliCPX-1933 DnaB-DnaG complex
CPX-1934 DnaB-DnaC complex
CPX-1935 DnaA-DnaB complex
CPX-1946 DnaB helicase complex
CPX-1950 DnaA-DnaB-DnaC complex
CPX-1951 DnaB-DnaC-DnaT-PriA-PriB complex
CPX-1952 DnaB-DnaC-DnaT-PriA-PriC complex
CPX-1953 DnaB-DnaC-Rep-PriC complex
DIPiDIP-9456N
IntActiP0ACB0, 39 interactors
MINTiP0ACB0
STRINGi316385.ECDH10B_4241

2D gel databases

SWISS-2DPAGEiP0ACB0

Proteomic databases

EPDiP0ACB0
PaxDbiP0ACB0
PRIDEiP0ACB0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77022; AAC77022; b4052
BAE78054; BAE78054; BAE78054
GeneIDi948555
KEGGiecj:JW4012
eco:b4052
PATRICifig|1411691.4.peg.2655

Organism-specific databases

EchoBASEiEB0232
EcoGeneiEG10236 dnaB

Phylogenomic databases

eggNOGiENOG4105CDU Bacteria
COG0305 LUCA
HOGENOMiHOG000113196
InParanoidiP0ACB0
KOiK02314
PhylomeDBiP0ACB0

Enzyme and pathway databases

BioCyciEcoCyc:EG10236-MONOMER
MetaCyc:EG10236-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0ACB0
PROiPR:P0ACB0

Family and domain databases

CDDicd00984 DnaB_C, 1 hit
Gene3Di1.10.860.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR036185 DNA_heli_DnaB-like_N_sf
IPR007692 DNA_helicase_DnaB
IPR007694 DNA_helicase_DnaB-like_C
IPR007693 DNA_helicase_DnaB-like_N
IPR016136 DNA_helicase_N/primase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00772 DnaB, 1 hit
PF03796 DnaB_C, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF48024 SSF48024, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00665 DnaB, 1 hit
PROSITEiView protein in PROSITE
PS51199 SF4_HELICASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDNAB_ECOLI
AccessioniPrimary (citable) accession number: P0ACB0
Secondary accession number(s): P03005, Q2M6Q2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: November 7, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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