UniProtKB - P0ACA7 (GSTB_ECOLI)
Protein
Glutathione S-transferase GstB
Gene
gstB
Organism
Escherichia coli (strain K12)
Status
Functioni
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes the glutathione-dependent dehalogenation of bromoacetate.1 Publication
Catalytic activityi
- EC:2.5.1.181 Publication
Kineticsi
- KM=5 mM for bromoacetate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 12 | GlutathioneBy similarity | 1 | |
Binding sitei | 39 | GlutathioneBy similarity | 1 | |
Binding sitei | 53 | Glutathione; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
GO - Molecular functioni
- arsenate reductase activity Source: EcoCyc
- glutathione binding Source: GO_Central
- glutathione transferase activity Source: EcoCyc
- identical protein binding Source: IntAct
GO - Biological processi
- glutathione metabolic process Source: InterPro
Keywordsi
Molecular function | Transferase |
Enzyme and pathway databases
BioCyci | EcoCyc:G6438-MONOMER MetaCyc:G6438-MONOMER |
SABIO-RKi | P0ACA7 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:gstB Synonyms:yliJ Ordered Locus Names:b0838, JW0822 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
Pathology & Biotechi
Disruption phenotypei
Mutants are hypersensitive to bromoacetate.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186018 | 1 – 208 | Glutathione S-transferase GstBAdd BLAST | 208 |
Proteomic databases
jPOSTi | P0ACA7 |
PaxDbi | P0ACA7 |
PRIDEi | P0ACA7 |
Interactioni
Binary interactionsi
Hide detailsP0ACA7
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-1120568,EBI-1120568 |
hisB [P06987] | 4 | EBI-1120568,EBI-1126930 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4262824, 14 interactors 849843, 2 interactors |
DIPi | DIP-48220N |
IntActi | P0ACA7, 2 interactors |
STRINGi | 511145.b0838 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 83 | GST N-terminalAdd BLAST | 83 | |
Domaini | 88 – 208 | GST C-terminalAdd BLAST | 121 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 67 – 68 | Glutathione bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the GST superfamily.Curated
Phylogenomic databases
eggNOGi | COG0625, Bacteria |
HOGENOMi | CLU_011226_6_2_6 |
InParanoidi | P0ACA7 |
PhylomeDBi | P0ACA7 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR004045, Glutathione_S-Trfase_N IPR004046, GST_C IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF00043, GST_C, 1 hit PF13417, GST_N_3, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit PS50404, GST_NTER, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ACA7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MITLWGRNNS TNVKKVLLTL EELELPYEQI LAGREFGINH DADFLAMNPN
60 70 80 90 100
GLVPLLRDDE SDLILWESNA IVRYLAAQYG QKRLWIDSPA RRAEAEKWMD
110 120 130 140 150
WANQTLSNAH RGILMGLVRT PPEERDQAAI DASCKECDAL FALLDAELAK
160 170 180 190 200
VKWFSGDEFG VGDIAIAPFI YNLFNVGLTW TPRPNLQRWY QQLTERPAVR
KVVMIPVS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73925.2 AP009048 Genomic DNA Translation: BAA35541.1 |
RefSeqi | NP_415359.4, NC_000913.3 WP_001295292.1, NZ_STEB01000019.1 |
Genome annotation databases
EnsemblBacteriai | AAC73925; AAC73925; b0838 BAA35541; BAA35541; BAA35541 |
GeneIDi | 58460681 945469 |
KEGGi | ecj:JW0822 eco:b0838 |
PATRICi | fig|1411691.4.peg.1440 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73925.2 AP009048 Genomic DNA Translation: BAA35541.1 |
RefSeqi | NP_415359.4, NC_000913.3 WP_001295292.1, NZ_STEB01000019.1 |
3D structure databases
SMRi | P0ACA7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262824, 14 interactors 849843, 2 interactors |
DIPi | DIP-48220N |
IntActi | P0ACA7, 2 interactors |
STRINGi | 511145.b0838 |
Proteomic databases
jPOSTi | P0ACA7 |
PaxDbi | P0ACA7 |
PRIDEi | P0ACA7 |
Genome annotation databases
EnsemblBacteriai | AAC73925; AAC73925; b0838 BAA35541; BAA35541; BAA35541 |
GeneIDi | 58460681 945469 |
KEGGi | ecj:JW0822 eco:b0838 |
PATRICi | fig|1411691.4.peg.1440 |
Organism-specific databases
EchoBASEi | EB3254 |
Phylogenomic databases
eggNOGi | COG0625, Bacteria |
HOGENOMi | CLU_011226_6_2_6 |
InParanoidi | P0ACA7 |
PhylomeDBi | P0ACA7 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6438-MONOMER MetaCyc:G6438-MONOMER |
SABIO-RKi | P0ACA7 |
Miscellaneous databases
PROi | PR:P0ACA7 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR004045, Glutathione_S-Trfase_N IPR004046, GST_C IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF00043, GST_C, 1 hit PF13417, GST_N_3, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit PS50404, GST_NTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GSTB_ECOLI | |
Accessioni | P0ACA7Primary (citable) accession number: P0ACA7 Secondary accession number(s): P75805, Q9R7R4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | February 10, 2021 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families