UniProtKB - P0AC84 (GLO2_ECOLI)
Protein
Hydroxyacylglutathione hydrolase GloB
Gene
gloB
Organism
Escherichia coli (strain K12)
Status
Functioni
Type II glyoxalase that catalyzes the hydrolysis of (R)-S-lactoylglutathione to (R)-lactate and glutathione (PubMed:25670698, PubMed:17196158). Is more efficient than the isozyme GloC, and plays a major contribution to methylglyoxal (MG) detoxification in E.coli (PubMed:25670698). The two isoenzymes have additive effects and ensure maximal MG degradation (PubMed:25670698).2 Publications
Catalytic activityi
Cofactori
Zn2+1 Publication1 PublicationNote: Binds 2 Zn2+ ions per subunit. Mn2+ and Co2+ can substitute for zinc in reconstitution experiments.1 Publication
Activity regulationi
Is inhibited by Cu2+.1 Publication
Kineticsi
kcat is 53 sec(-1) for the hydrolysis of (R)-S-lactoylglutathione (PubMed:17196158). kcat is 15.6 sec(-1) for the hydrolysis of (R)-S-lactoylglutathione (PubMed:25670698).2 Publications
- KM=184 µM for (R)-S-lactoylglutathione1 Publication
- KM=0.5 mM for (R)-S-lactoylglutathione1 Publication
- Vmax=112 µmol/min/mg enzyme for the hydrolysis of (R)-S-lactoylglutathione1 Publication
: methylglyoxal degradation Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.1 PublicationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Lactoylglutathione lyase (gloA), Lactoylglutathione lyase (gloA)
- Hydroxyacylglutathione hydrolase (gloB), Hydroxyacylglutathione hydrolase GloC (gloC), Hydroxyacylglutathione hydrolase GloB (gloB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 53 | Zinc 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 55 | Zinc 1; via pros nitrogenBy similarity | 1 | |
Metal bindingi | 57 | Zinc 2By similarity | 1 | |
Metal bindingi | 58 | Zinc 2; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 110 | Zinc 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 127 | Zinc 1By similarity | 1 | |
Metal bindingi | 127 | Zinc 2By similarity | 1 | |
Metal bindingi | 165 | Zinc 2; via tele nitrogenBy similarity | 1 |
GO - Molecular functioni
- hydroxyacylglutathione hydrolase activity Source: EcoCyc
- zinc ion binding Source: EcoCyc
GO - Biological processi
- methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: EcoCyc
- response to heat Source: EcoCyc
- response to toxic substance Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Detoxification |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:GLYOXII-MONOMER MetaCyc:GLYOXII-MONOMER |
BRENDAi | 3.1.2.6, 2026 |
SABIO-RKi | P0AC84 |
UniPathwayi | UPA00619;UER00676 |
Names & Taxonomyi
Protein namesi | Recommended name: Hydroxyacylglutathione hydrolase GloB2 Publications (EC:3.1.2.62 Publications)Alternative name(s): Glyoxalase II2 Publications Short name: Glx II2 Publications |
Gene namesi | Name:gloB1 Publication Synonyms:yafR Ordered Locus Names:b0212, JW0202 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene show decreased methylglyoxal tolerance. A double deletion mutant lacking both gloB and gloC exhibits almost no resistance to exogenously supplied methylglyoxal, and is unable to grow at MG concentrations as low as 0.1 mM.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000192351 | 1 – 251 | Hydroxyacylglutathione hydrolase GloBAdd BLAST | 251 |
Proteomic databases
jPOSTi | P0AC84 |
PaxDbi | P0AC84 |
PRIDEi | P0AC84 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262005, 24 interactors |
DIPi | DIP-48248N |
IntActi | P0AC84, 1 interactor |
STRINGi | 511145.b0212 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AC84 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 136 – 138 | Substrate bindingBy similarity | 3 | |
Regioni | 165 – 167 | Substrate bindingBy similarity | 3 | |
Regioni | 245 – 248 | Substrate bindingBy similarity | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0491, Bacteria |
HOGENOMi | CLU_030571_4_1_6 |
InParanoidi | P0AC84 |
PhylomeDBi | P0AC84 |
Family and domain databases
CDDi | cd07723, hydroxyacylglutathione_hydrolase_MBL-fold, 1 hit |
Gene3Di | 3.60.15.10, 1 hit |
HAMAPi | MF_01374, Glyoxalase_2, 1 hit |
InterProi | View protein in InterPro IPR035680, Clx_II_MBL IPR032282, HAGH_C IPR017782, Hydroxyacylglutathione_Hdrlase IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro |
Pfami | View protein in Pfam PF16123, HAGH_C, 1 hit PF00753, Lactamase_B, 1 hit |
PIRSFi | PIRSF005457, Glx, 1 hit |
SMARTi | View protein in SMART SM00849, Lactamase_B, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR03413, GSH_gloB, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AC84-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNLNSIPAFD DNYIWVLNDE AGRCLIVDPG DAEPVLNAIA ANNWQPEAIF
60 70 80 90 100
LTHHHHDHVG GVKELVEKFP QIVVYGPQET QDKGTTQVVK DGETAFVLGH
110 120 130 140 150
EFSVIATPGH TLGHICYFSK PYLFCGDTLF SGGCGRLFEG TASQMYQSLK
160 170 180 190 200
KLSALPDDTL VCCAHEYTLS NMKFALSILP HDLSINDYYR KVKELRAKNQ
210 220 230 240 250
ITLPVILKNE RQINVFLRTE DIDLINVINE ETLLQQPEER FAWLRSKKDR
F
Mass spectrometryi
Molecular mass is 28432 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U70214 Genomic DNA Translation: AAB08634.1 U00096 Genomic DNA Translation: AAC73317.1 AP009048 Genomic DNA Translation: BAA77883.1 |
PIRi | F64745 |
RefSeqi | NP_414748.1, NC_000913.3 WP_001052715.1, NZ_SSZK01000029.1 |
Genome annotation databases
EnsemblBacteriai | AAC73317; AAC73317; b0212 BAA77883; BAA77883; BAA77883 |
GeneIDi | 57729485 944902 |
KEGGi | ecj:JW0202 eco:b0212 |
PATRICi | fig|1411691.4.peg.2072 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U70214 Genomic DNA Translation: AAB08634.1 U00096 Genomic DNA Translation: AAC73317.1 AP009048 Genomic DNA Translation: BAA77883.1 |
PIRi | F64745 |
RefSeqi | NP_414748.1, NC_000913.3 WP_001052715.1, NZ_SSZK01000029.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6RZ0 | X-ray | 1.10 | A | 1-251 | [»] | |
6S0I | X-ray | 1.80 | A | 1-251 | [»] | |
SMRi | P0AC84 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262005, 24 interactors |
DIPi | DIP-48248N |
IntActi | P0AC84, 1 interactor |
STRINGi | 511145.b0212 |
Proteomic databases
jPOSTi | P0AC84 |
PaxDbi | P0AC84 |
PRIDEi | P0AC84 |
Genome annotation databases
EnsemblBacteriai | AAC73317; AAC73317; b0212 BAA77883; BAA77883; BAA77883 |
GeneIDi | 57729485 944902 |
KEGGi | ecj:JW0202 eco:b0212 |
PATRICi | fig|1411691.4.peg.2072 |
Organism-specific databases
EchoBASEi | EB3114 |
Phylogenomic databases
eggNOGi | COG0491, Bacteria |
HOGENOMi | CLU_030571_4_1_6 |
InParanoidi | P0AC84 |
PhylomeDBi | P0AC84 |
Enzyme and pathway databases
UniPathwayi | UPA00619;UER00676 |
BioCyci | EcoCyc:GLYOXII-MONOMER MetaCyc:GLYOXII-MONOMER |
BRENDAi | 3.1.2.6, 2026 |
SABIO-RKi | P0AC84 |
Miscellaneous databases
PROi | PR:P0AC84 |
Family and domain databases
CDDi | cd07723, hydroxyacylglutathione_hydrolase_MBL-fold, 1 hit |
Gene3Di | 3.60.15.10, 1 hit |
HAMAPi | MF_01374, Glyoxalase_2, 1 hit |
InterProi | View protein in InterPro IPR035680, Clx_II_MBL IPR032282, HAGH_C IPR017782, Hydroxyacylglutathione_Hdrlase IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro |
Pfami | View protein in Pfam PF16123, HAGH_C, 1 hit PF00753, Lactamase_B, 1 hit |
PIRSFi | PIRSF005457, Glx, 1 hit |
SMARTi | View protein in SMART SM00849, Lactamase_B, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR03413, GSH_gloB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLO2_ECOLI | |
Accessioni | P0AC84Primary (citable) accession number: P0AC84 Secondary accession number(s): Q47677 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | April 7, 2021 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families