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UniProtKB - P0AC81 (LGUL_ECOLI)
Protein
Lactoylglutathione lyase
Gene
gloA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the isomerization of the hemithioacetal formed spontaneously from methylglyoxal and glutathione, to S-lactoylglutathione, which is then hydrolyzed by a type II glyoxalase (GloB or GloC). Is involved in methylglyoxal (MG) detoxification (PubMed:10913283) (Probable).
Involved in resistance to hypochlorous acid (HOCl), which is the active component of household bleach and a powerful antimicrobial during the innate immune response (PubMed:23536188).
1 Publication2 PublicationsCatalytic activityi
- EC:4.4.1.51 Publication
Cofactori
Ni2+1 PublicationNote: Binds 1 nickel ion per subunit. In the homodimer, two nickel ions are bound between subunits. Is not active with zinc ions.1 Publication
: methylglyoxal degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (R)-lactate from methylglyoxal.1 Publication This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 5 | Nickel; via tele nitrogen; shared with dimeric partner1 Publication | 1 | |
Binding sitei | 9 | Substrate; shared with dimeric partnerBy similarity | 1 | |
Metal bindingi | 56 | Nickel; shared with dimeric partner1 Publication | 1 | |
Binding sitei | 60 | Substrate; shared with dimeric partnerBy similarity | 1 | |
Metal bindingi | 74 | Nickel; via tele nitrogen1 Publication | 1 | |
Binding sitei | 74 | SubstrateBy similarity | 1 | |
Active sitei | 122 | Proton donor/acceptorBy similarity | 1 | |
Metal bindingi | 122 | Nickel1 Publication | 1 |
GO - Molecular functioni
- lactoylglutathione lyase activity Source: EcoCyc
- nickel cation binding Source: EcoCyc
GO - Biological processi
- methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: EcoCyc
- response to toxic substance Source: UniProtKB-KW
Keywordsi
Molecular function | Lyase |
Biological process | Detoxification |
Ligand | Metal-binding, Nickel |
Enzyme and pathway databases
BioCyci | EcoCyc:GLYOXI-MONOMER |
BRENDAi | 4.4.1.5, 2026 |
SABIO-RKi | P0AC81 |
UniPathwayi | UPA00619;UER00675 |
Names & Taxonomyi
Protein namesi | Recommended name: Lactoylglutathione lyase (EC:4.4.1.5)Alternative name(s): Aldoketomutase Glyoxalase I1 Publication Short name: Glx I1 Publication Ketone-aldehyde mutase Methylglyoxalase S-D-lactoylglutathione methylglyoxal lyase |
Gene namesi | Name:gloA Ordered Locus Names:b1651, JW1643 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Pathology & Biotechi
Disruption phenotypei
Deletion of the gene increases the HOCl sensitivity. Mutant is more sensitive to methylglyoxal treatment.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000168088 | 1 – 135 | Lactoylglutathione lyaseAdd BLAST | 135 |
Proteomic databases
jPOSTi | P0AC81 |
PaxDbi | P0AC81 |
PRIDEi | P0AC81 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
P0AC81
With | #Exp. | IntAct |
---|---|---|
dnaK [P0A6Y8] | 3 | EBI-551143,EBI-542092 |
Protein-protein interaction databases
BioGRIDi | 4259387, 22 interactors 850521, 1 interactor |
DIPi | DIP-47995N |
IntActi | P0AC81, 7 interactors |
STRINGi | 511145.b1651 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AC81 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AC81 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 126 | VOCPROSITE-ProRule annotationAdd BLAST | 125 |
Sequence similaritiesi
Belongs to the glyoxalase I family.Curated
Phylogenomic databases
eggNOGi | COG0346, Bacteria |
HOGENOMi | CLU_046006_8_1_6 |
InParanoidi | P0AC81 |
PhylomeDBi | P0AC81 |
Family and domain databases
Gene3Di | 3.10.180.10, 1 hit |
InterProi | View protein in InterPro IPR029068, Glyas_Bleomycin-R_OHBP_Dase IPR004360, Glyas_Fos-R_dOase_dom IPR004361, Glyoxalase_1 IPR018146, Glyoxalase_1_CS IPR037523, VOC |
Pfami | View protein in Pfam PF00903, Glyoxalase, 1 hit |
SUPFAMi | SSF54593, SSF54593, 1 hit |
TIGRFAMsi | TIGR00068, glyox_I, 1 hit |
PROSITEi | View protein in PROSITE PS00934, GLYOXALASE_I_1, 1 hit PS00935, GLYOXALASE_I_2, 1 hit PS51819, VOC, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AC81-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLLHTMLRV GDLQRSIDFY TKVLGMKLLR TSENPEYKYS LAFVGYGPET
60 70 80 90 100
EEAVIELTYN WGVDKYELGT AYGHIALSVD NAAEACEKIR QNGGNVTREA
110 120 130
GPVKGGTTVI AFVEDPDGYK IELIEEKDAG RGLGN
Mass spectrometryi
Molecular mass is 14919 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U57363 Genomic DNA Translation: AAC27133.1 U00096 Genomic DNA Translation: AAC74723.1 AP009048 Genomic DNA Translation: BAE76494.1 D86931 Genomic DNA Translation: BAA13187.1 |
PIRi | E64922 |
RefSeqi | NP_416168.1, NC_000913.3 WP_001237796.1, NZ_STEB01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC74723; AAC74723; b1651 BAE76494; BAE76494; BAE76494 |
GeneIDi | 66674457 946161 |
KEGGi | ecj:JW1643 eco:b1651 |
PATRICi | fig|1411691.4.peg.608 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U57363 Genomic DNA Translation: AAC27133.1 U00096 Genomic DNA Translation: AAC74723.1 AP009048 Genomic DNA Translation: BAE76494.1 D86931 Genomic DNA Translation: BAA13187.1 |
PIRi | E64922 |
RefSeqi | NP_416168.1, NC_000913.3 WP_001237796.1, NZ_STEB01000003.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1F9Z | X-ray | 1.50 | A/B | 1-135 | [»] | |
1FA5 | X-ray | 1.80 | A/B | 1-135 | [»] | |
1FA6 | X-ray | 1.90 | A/B | 1-135 | [»] | |
1FA7 | X-ray | 1.90 | A/B | 1-135 | [»] | |
1FA8 | X-ray | 1.70 | A/B | 1-135 | [»] | |
SMRi | P0AC81 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259387, 22 interactors 850521, 1 interactor |
DIPi | DIP-47995N |
IntActi | P0AC81, 7 interactors |
STRINGi | 511145.b1651 |
Proteomic databases
jPOSTi | P0AC81 |
PaxDbi | P0AC81 |
PRIDEi | P0AC81 |
Genome annotation databases
EnsemblBacteriai | AAC74723; AAC74723; b1651 BAE76494; BAE76494; BAE76494 |
GeneIDi | 66674457 946161 |
KEGGi | ecj:JW1643 eco:b1651 |
PATRICi | fig|1411691.4.peg.608 |
Organism-specific databases
EchoBASEi | EB3197 |
Phylogenomic databases
eggNOGi | COG0346, Bacteria |
HOGENOMi | CLU_046006_8_1_6 |
InParanoidi | P0AC81 |
PhylomeDBi | P0AC81 |
Enzyme and pathway databases
UniPathwayi | UPA00619;UER00675 |
BioCyci | EcoCyc:GLYOXI-MONOMER |
BRENDAi | 4.4.1.5, 2026 |
SABIO-RKi | P0AC81 |
Miscellaneous databases
EvolutionaryTracei | P0AC81 |
PROi | PR:P0AC81 |
Family and domain databases
Gene3Di | 3.10.180.10, 1 hit |
InterProi | View protein in InterPro IPR029068, Glyas_Bleomycin-R_OHBP_Dase IPR004360, Glyas_Fos-R_dOase_dom IPR004361, Glyoxalase_1 IPR018146, Glyoxalase_1_CS IPR037523, VOC |
Pfami | View protein in Pfam PF00903, Glyoxalase, 1 hit |
SUPFAMi | SSF54593, SSF54593, 1 hit |
TIGRFAMsi | TIGR00068, glyox_I, 1 hit |
PROSITEi | View protein in PROSITE PS00934, GLYOXALASE_I_1, 1 hit PS00935, GLYOXALASE_I_2, 1 hit PS51819, VOC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LGUL_ECOLI | |
Accessioni | P0AC81Primary (citable) accession number: P0AC81 Secondary accession number(s): P77036, Q2MB62, Q59384 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | February 23, 2022 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families