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Protein

3-deoxy-D-manno-octulosonic acid transferase

Gene

waaA

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of two 3-deoxy-D-manno-octulosonate (Kdo) residues from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A.By similarity

Catalytic activityi

Lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->6)-lipid IV(A) + CMP.
Alpha-Kdo-(2->6)-lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP.

Pathwayi: KDO(2)-lipid A biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A).
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 3-deoxy-D-manno-octulosonic acid transferase (waaA)
  2. 3-deoxy-D-manno-octulosonic acid transferase (waaA)
  3. Lipid A biosynthesis lauroyltransferase (htrB)
  4. Lipid A biosynthesis myristoyltransferase (msbB)
This subpathway is part of the pathway KDO(2)-lipid A biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A), the pathway KDO(2)-lipid A biosynthesis and in Glycolipid biosynthesis.

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei60Proton acceptorBy similarity1
Sitei130Transition state stabilizerBy similarity1
Sitei208Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processLipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciECOL199310:C4457-MONOMER
UniPathwayi
UPA00360;UER00483

UPA00360;UER00484

UPA00958

Protein family/group databases

CAZyiGT30 Glycosyltransferase Family 30

Names & Taxonomyi

Protein namesi
Recommended name:
3-deoxy-D-manno-octulosonic acid transferase (EC:2.4.99.12, EC:2.4.99.13)
Short name:
Kdo transferase
Alternative name(s):
Bifunctional Kdo transferase
Kdo-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Gene namesi
Name:waaA
Synonyms:kdtA
Ordered Locus Names:c4457
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001410 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei3 – 23Helical; Signal-anchorSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802881 – 4253-deoxy-D-manno-octulosonic acid transferaseAdd BLAST425

Interactioni

Protein-protein interaction databases

STRINGi199310.c4457

Structurei

3D structure databases

ProteinModelPortaliP0AC76
SMRiP0AC76
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni268 – 269CMP-Kdo bindingBy similarity2
Regioni309 – 311CMP-Kdo bindingBy similarity3
Regioni335 – 338CMP-Kdo bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D8A Bacteria
COG1519 LUCA
HOGENOMiHOG000257156
KOiK02527
OMAiFIKYEFW

Family and domain databases

Gene3Di3.40.50.11720, 1 hit
InterProiView protein in InterPro
IPR001296 Glyco_trans_1
IPR007507 Glycos_transf_N
IPR038107 Glycos_transf_N_sf
PfamiView protein in Pfam
PF00534 Glycos_transf_1, 1 hit
PF04413 Glycos_transf_N, 1 hit

Sequencei

Sequence statusi: Complete.

P0AC76-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLELLYTALL YLIQPLIWIR LWVRGRKAPA YRKRWGERYG FYRHPLKPGG
60 70 80 90 100
IMLHSVSVGE TLAAIPLVRA LRHRYPDLPI TVTTMTPTGS ERVQSAFGKD
110 120 130 140 150
VQHVYLPYDL PDALNRFLNK VDPKLVLIME TELWPNLIAA LHKRKIPLVI
160 170 180 190 200
ANARLSARSA AGYAKLGKFV RRLLRRITLI AAQNEEDGAR FVALGAKNNQ
210 220 230 240 250
VTVTGSLKFD ISVTPQLAAK AVTLRRQWAP HRPVWIATST HEGEESVVIA
260 270 280 290 300
AHQALLQQFP NLLLILVPRH PERFPDAINL VRQAGLSYIT RSSGEVPSTS
310 320 330 340 350
TQVVVGDTMG ELMLLYGIAD LAFVGGSLVE RGGHNPLEAA AHAIPVLMGP
360 370 380 390 400
HTFNFKDICA RLEQASGLIT VTDATTLAKE VSSLLTDADY RSFYGRHAVE
410 420
VLYQNQGALQ RLLQLLEPYL PPKTH
Length:425
Mass (Da):47,291
Last modified:November 8, 2005 - v1
Checksum:iB063850A1FF392AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA Translation: AAN82893.1
RefSeqiWP_000891564.1, NC_004431.1

Genome annotation databases

EnsemblBacteriaiAAN82893; AAN82893; c4457
KEGGiecc:c4457

Similar proteinsi

Entry informationi

Entry nameiKDTA_ECOL6
AccessioniPrimary (citable) accession number: P0AC76
Secondary accession number(s): P23282
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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