UniProtKB - P0AC75 (KDTA_ECOLI)
Protein
3-deoxy-D-manno-octulosonic acid transferase
Gene
waaA
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of two 3-deoxy-D-manno-octulosonate (Kdo) residues from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A.2 Publications
Catalytic activityi
- CMP-3-deoxy-β-D-manno-octulosonate + lipid IVA (E. coli) = α-Kdo-(2→6)-lipid IVA + CMP + H+2 PublicationsEC:2.4.99.122 Publications
- α-Kdo-(2→6)-lipid IVA + CMP-3-deoxy-β-D-manno-octulosonate = α-Kdo-(2→4)-α-Kdo-(2→6)-lipid IVA + CMP + H+2 PublicationsEC:2.4.99.132 Publications
Activity regulationi
Catalytic activity is inhibited by the antibiotic polymixin B and by Re endotoxin.1 Publication
Kineticsi
- KM=52 µM for lipid IV(A) (at pH 8)1 Publication
- KM=88 µM for CMP-Kdo (at pH 8)1 Publication
- Vmax=18 µmol/min/mg enzyme (at pH 8)1 Publication
pH dependencei
Optimum pH is 7.1 Publication
: KDO(2)-lipid A biosynthesis Pathwayi
This protein is involved in step 1 and 2 of the subpathway that synthesizes KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A).1 PublicationProteins known to be involved in the 4 steps of the subpathway in this organism are:
- 3-deoxy-D-manno-octulosonic acid transferase (waaA)
- 3-deoxy-D-manno-octulosonic acid transferase (waaA)
- Lipid A biosynthesis lauroyltransferase (lpxL), Lipid A biosynthesis lauroyltransferase (lpxL)
- Lipid A biosynthesis myristoyltransferase (lpxM), Lipid A biosynthesis myristoyltransferase (msbB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A), the pathway KDO(2)-lipid A biosynthesis and in Glycolipid biosynthesis.
Pathwayi: LPS core biosynthesis
This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 60 | Proton acceptorBy similarity | 1 | |
Sitei | 130 | Transition state stabilizerBy similarity | 1 | |
Sitei | 208 | Transition state stabilizerBy similarity | 1 |
GO - Molecular functioni
- Kdo transferase activity Source: UniProtKB-EC
- transferase activity Source: EcoCyc
GO - Biological processi
- Kdo2-lipid A biosynthetic process Source: UniProtKB-UniPathway
- lipid A biosynthetic process Source: EcoliWiki
- lipopolysaccharide core region biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | Lipopolysaccharide biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:KDOTRANS-MONOMER MetaCyc:KDOTRANS-MONOMER |
BRENDAi | 2.4.99.12, 2026 2.4.99.13, 2026 |
SABIO-RKi | P0AC75 |
UniPathwayi | UPA00360;UER00483 UPA00360;UER00484 UPA00958 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-deoxy-D-manno-octulosonic acid transferase (EC:2.4.99.122 Publications, EC:2.4.99.132 Publications)Short name: Kdo transferase Alternative name(s): Bifunctional Kdo transferase Kdo-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase |
Gene namesi | Name:waaA Synonyms:kdtA Ordered Locus Names:b3633, JW3608 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Single-pass membrane protein 1 Publication; Cytoplasmic side 1 Publication
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: EcoliWiki
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 3 – 23 | Helical; Signal-anchorSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Cells lacking this gene display growth defects, absence of Kdo transferase activity, and accumulate massive amounts of lipid IV(A).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080286 | 1 – 425 | 3-deoxy-D-manno-octulosonic acid transferaseAdd BLAST | 425 |
Post-translational modificationi
Degraded by the protease FtsH; therefore FtsH regulates the addition of the sugar moiety to the LPS and thus the maturation of the LPS precursor.1 Publication
Proteomic databases
jPOSTi | P0AC75 |
PaxDbi | P0AC75 |
PRIDEi | P0AC75 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263240, 354 interactors 853292, 1 interactor |
DIPi | DIP-48036N |
IntActi | P0AC75, 16 interactors |
STRINGi | 511145.b3633 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 268 – 269 | CMP-Kdo bindingBy similarity | 2 | |
Regioni | 309 – 311 | CMP-Kdo bindingBy similarity | 3 | |
Regioni | 335 – 338 | CMP-Kdo bindingBy similarity | 4 |
Domaini
The N-terminal half of KdtA is responsible for determining the number of Kdo residues that are transferred to lipid IVA.1 Publication
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1519, Bacteria |
HOGENOMi | CLU_036146_2_0_6 |
InParanoidi | P0AC75 |
PhylomeDBi | P0AC75 |
Family and domain databases
Gene3Di | 3.40.50.11720, 1 hit |
InterProi | View protein in InterPro IPR007507, Glycos_transf_N IPR038107, Glycos_transf_N_sf IPR039901, Kdotransferase |
PANTHERi | PTHR42755, PTHR42755, 1 hit |
Pfami | View protein in Pfam PF04413, Glycos_transf_N, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AC75-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLELLYTALL YLIQPLIWIR LWVRGRKAPA YRKRWGERYG FYRHPLKPGG
60 70 80 90 100
IMLHSVSVGE TLAAIPLVRA LRHRYPDLPI TVTTMTPTGS ERVQSAFGKD
110 120 130 140 150
VQHVYLPYDL PDALNRFLNK VDPKLVLIME TELWPNLIAA LHKRKIPLVI
160 170 180 190 200
ANARLSARSA AGYAKLGKFV RRLLRRITLI AAQNEEDGAR FVALGAKNNQ
210 220 230 240 250
VTVTGSLKFD ISVTPQLAAK AVTLRRQWAP HRPVWIATST HEGEESVVIA
260 270 280 290 300
AHQALLQQFP NLLLILVPRH PERFPDAINL VRQAGLSYIT RSSGEVPSTS
310 320 330 340 350
TQVVVGDTMG ELMLLYGIAD LAFVGGSLVE RGGHNPLEAA AHAIPVLMGP
360 370 380 390 400
HTFNFKDICA RLEQASGLIT VTDATTLAKE VSSLLTDADY RSFYGRHAVE
410 420
VLYQNQGALQ RLLQLLEPYL PPKTH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M60670 Genomic DNA Translation: AAA24043.1 M86305 Genomic DNA Translation: AAA03745.1 U00039 Genomic DNA Translation: AAB18610.1 U00096 Genomic DNA Translation: AAC76657.1 AP009048 Genomic DNA Translation: BAE77659.1 |
PIRi | JU0467 |
RefSeqi | NP_418090.1, NC_000913.3 WP_000891564.1, NZ_STEB01000024.1 |
Genome annotation databases
EnsemblBacteriai | AAC76657; AAC76657; b3633 BAE77659; BAE77659; BAE77659 |
GeneIDi | 58461614 949048 |
KEGGi | ecj:JW3608 eco:b3633 |
PATRICi | fig|1411691.4.peg.3073 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M60670 Genomic DNA Translation: AAA24043.1 M86305 Genomic DNA Translation: AAA03745.1 U00039 Genomic DNA Translation: AAB18610.1 U00096 Genomic DNA Translation: AAC76657.1 AP009048 Genomic DNA Translation: BAE77659.1 |
PIRi | JU0467 |
RefSeqi | NP_418090.1, NC_000913.3 WP_000891564.1, NZ_STEB01000024.1 |
3D structure databases
SMRi | P0AC75 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263240, 354 interactors 853292, 1 interactor |
DIPi | DIP-48036N |
IntActi | P0AC75, 16 interactors |
STRINGi | 511145.b3633 |
Proteomic databases
jPOSTi | P0AC75 |
PaxDbi | P0AC75 |
PRIDEi | P0AC75 |
Genome annotation databases
EnsemblBacteriai | AAC76657; AAC76657; b3633 BAE77659; BAE77659; BAE77659 |
GeneIDi | 58461614 949048 |
KEGGi | ecj:JW3608 eco:b3633 |
PATRICi | fig|1411691.4.peg.3073 |
Organism-specific databases
EchoBASEi | EB0515 |
Phylogenomic databases
eggNOGi | COG1519, Bacteria |
HOGENOMi | CLU_036146_2_0_6 |
InParanoidi | P0AC75 |
PhylomeDBi | P0AC75 |
Enzyme and pathway databases
UniPathwayi | UPA00360;UER00483 UPA00360;UER00484 UPA00958 |
BioCyci | EcoCyc:KDOTRANS-MONOMER MetaCyc:KDOTRANS-MONOMER |
BRENDAi | 2.4.99.12, 2026 2.4.99.13, 2026 |
SABIO-RKi | P0AC75 |
Miscellaneous databases
PROi | PR:P0AC75 |
Family and domain databases
Gene3Di | 3.40.50.11720, 1 hit |
InterProi | View protein in InterPro IPR007507, Glycos_transf_N IPR038107, Glycos_transf_N_sf IPR039901, Kdotransferase |
PANTHERi | PTHR42755, PTHR42755, 1 hit |
Pfami | View protein in Pfam PF04413, Glycos_transf_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KDTA_ECOLI | |
Accessioni | P0AC75Primary (citable) accession number: P0AC75 Secondary accession number(s): P23282, Q2M7U7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | February 10, 2021 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families