UniProtKB - P0AC69 (GLRX4_ECOLI)
Protein
Glutaredoxin 4
Gene
grxD
Organism
Escherichia coli (strain K12)
Status
Functioni
Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 22 | Glutathione1 Publication | 1 | |
Metal bindingi | 30 | Iron-sulfur (2Fe-2S); shared with dimeric partner | 1 | |
Binding sitei | 59 | Glutathione1 Publication | 1 | |
Binding sitei | 71 | Glutathione; via amide nitrogen and carbonyl oxygen1 Publication | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
- protein disulfide oxidoreductase activity Source: InterPro
Keywordsi
Ligand | 2Fe-2S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12181-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Glutaredoxin 4Short name: Grx4 Alternative name(s): Monothiol glutaredoxin |
Gene namesi | Name:grxD Synonyms:ydhD Ordered Locus Names:b1654, JW1646 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000102257 | 1 – 115 | Glutaredoxin 4Add BLAST | 115 |
Proteomic databases
jPOSTi | P0AC69 |
PaxDbi | P0AC69 |
PRIDEi | P0AC69 |
2D gel databases
SWISS-2DPAGEi | P0AC69 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 4260269, 437 interactors |
ComplexPortali | CPX-5898, bolA-grxD Fe-S cluster assembly complex CPX-5899, ibaG-grxD Fe-S cluster assembly complex |
DIPi | DIP-11729N |
IntActi | P0AC69, 19 interactors |
STRINGi | 511145.b1654 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AC69 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AC69 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 107 | GlutaredoxinPROSITE-ProRule annotationAdd BLAST | 103 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 84 – 85 | Glutathione binding | 2 |
Sequence similaritiesi
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | COG0278, Bacteria |
HOGENOMi | CLU_026126_2_1_6 |
InParanoidi | P0AC69 |
PhylomeDBi | P0AC69 |
Family and domain databases
CDDi | cd03028, GRX_PICOT_like, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR002109, Glutaredoxin IPR033658, GRX_PICOT-like IPR014434, Monothiol_GRX IPR004480, Monothiol_GRX-rel IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR10293, PTHR10293, 1 hit |
Pfami | View protein in Pfam PF00462, Glutaredoxin, 1 hit |
PIRSFi | PIRSF005894, Monothiol_GRX, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR00365, TIGR00365, 1 hit |
PROSITEi | View protein in PROSITE PS51354, GLUTAREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AC69-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTTIEKIQR QIAENPILLY MKGSPKLPSC GFSAQAVQAL AACGERFAYV
60 70 80 90 100
DILQNPDIRA ELPKYANWPT FPQLWVDGEL VGGCDIVIEM YQRGELQQLI
110
KETAAKYKSE EPDAE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74726.1 AP009048 Genomic DNA Translation: BAA15420.1 L01622 Genomic DNA Translation: AAC37010.1 |
PIRi | H64922 |
RefSeqi | NP_416171.1, NC_000913.3 WP_000108172.1, NZ_STEB01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC74726; AAC74726; b1654 BAA15420; BAA15420; BAA15420 |
GeneIDi | 58462457 946169 |
KEGGi | ecj:JW1646 eco:b1654 |
PATRICi | fig|1411691.4.peg.605 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74726.1 AP009048 Genomic DNA Translation: BAA15420.1 L01622 Genomic DNA Translation: AAC37010.1 |
PIRi | H64922 |
RefSeqi | NP_416171.1, NC_000913.3 WP_000108172.1, NZ_STEB01000003.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YKA | NMR | - | A | 1-115 | [»] | |
2WCI | X-ray | 1.90 | A/B | 1-115 | [»] | |
SMRi | P0AC69 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260269, 437 interactors |
ComplexPortali | CPX-5898, bolA-grxD Fe-S cluster assembly complex CPX-5899, ibaG-grxD Fe-S cluster assembly complex |
DIPi | DIP-11729N |
IntActi | P0AC69, 19 interactors |
STRINGi | 511145.b1654 |
2D gel databases
SWISS-2DPAGEi | P0AC69 |
Proteomic databases
jPOSTi | P0AC69 |
PaxDbi | P0AC69 |
PRIDEi | P0AC69 |
Genome annotation databases
EnsemblBacteriai | AAC74726; AAC74726; b1654 BAA15420; BAA15420; BAA15420 |
GeneIDi | 58462457 946169 |
KEGGi | ecj:JW1646 eco:b1654 |
PATRICi | fig|1411691.4.peg.605 |
Organism-specific databases
EchoBASEi | EB2098 |
Phylogenomic databases
eggNOGi | COG0278, Bacteria |
HOGENOMi | CLU_026126_2_1_6 |
InParanoidi | P0AC69 |
PhylomeDBi | P0AC69 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12181-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AC69 |
PROi | PR:P0AC69 |
Family and domain databases
CDDi | cd03028, GRX_PICOT_like, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR002109, Glutaredoxin IPR033658, GRX_PICOT-like IPR014434, Monothiol_GRX IPR004480, Monothiol_GRX-rel IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR10293, PTHR10293, 1 hit |
Pfami | View protein in Pfam PF00462, Glutaredoxin, 1 hit |
PIRSFi | PIRSF005894, Monothiol_GRX, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR00365, TIGR00365, 1 hit |
PROSITEi | View protein in PROSITE PS51354, GLUTAREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLRX4_ECOLI | |
Accessioni | P0AC69Primary (citable) accession number: P0AC69 Secondary accession number(s): P37010, P77424 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | April 7, 2021 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families