UniProtKB - P0AC69 (GLRX4_ECOLI)

BLAST

>sp|P0AC69|GLRX4_ECOLI Glutaredoxin 4 OS=Escherichia coli (strain K12) OX=83333 GN=grxD PE=1 SV=1
MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACGERFAYVDILQNPDIRA
ELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKETAAKYKSEEPDAE

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History
Entry version 99 (28 Mar 2018)
Sequence version 1 (08 Nov 2005)
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Protein

Glutaredoxin 4

Gene

grxD

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters.Curated

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	22	Glutathione1 Publication	1
Metal bindingⁱ	30	Iron-sulfur (2Fe-2S); shared with dimeric partner	1
Binding siteⁱ	59	Glutathione1 Publication	1
Binding siteⁱ	71	Glutathione; via amide nitrogen and carbonyl oxygen1 Publication	1

GO - Molecular functionⁱ

2 iron, 2 sulfur cluster binding
Source: EcoCyc
Inferred from direct assayⁱ
- 19505088
electron transfer activity Source: InterPro
metal ion binding Source: UniProtKB-KW
protein disulfide oxidoreductase activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell redox homeostasis Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Ligand	2Fe-2S, Iron, Iron-sulfur, Metal-binding

Ligand

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG12181-MONOMER

BioCycⁱ

EcoCyc:EG12181-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Glutaredoxin 4 Short name: Grx4 Alternative name(s): Monothiol glutaredoxin
Gene namesⁱ	Name:grxD Synonyms:ydhD Ordered Locus Names:b1654, JW1646
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG12181 grxD

EcoGeneⁱ

EG12181 grxD

Subcellular locationⁱ

Cytoplasm
1 Publication
Manual assertion based on experiment inⁱ
- Ref.5
  "A novel monothiol glutaredoxin (Grx4) from Escherichia coli can serve as a substrate for thioredoxin reductase."
  Fernandes A.P., Fladvad M., Berndt C., Andresen C., Lillig C.H., Neubauer P., Sunnerhagen M., Holmgren A., Vlamis-Gardikas A.
  J. Biol. Chem. 280:24544-24552(2005) [PubMed] [Europe PMC] [Abstract]
  Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.

GO - Cellular componentⁱ

cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 15911532
- 18304323

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000102257	1 – 115	Glutaredoxin 4Add BLAST		115

Proteomic databases

EPDⁱ	P0AC69
PaxDbⁱ	P0AC69
PRIDEⁱ	P0AC69

2D gel databases

SWISS-2DPAGEⁱ	P0AC69

SWISS-2DPAGEⁱ

P0AC69

Interactionⁱ

Subunit structureⁱ

Homodimer.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
bolA	P0ABE2	4	EBI-545828,EBI-545774

With

Entry

#Exp.

IntAct

Notes

bolA

P0ABE2

EBI-545828,EBI-545774

Protein-protein interaction databases

BioGridⁱ	4260269, 437 interactors
Database of interacting proteins More...DIPⁱ	DIP-11729N
IntActⁱ	P0AC69, 18 interactors
STRINGⁱ	316385.ECDH10B_1788

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	3 – 14	Combined sources	12
Beta strandⁱ	16 – 23	Combined sources	8
Beta strandⁱ	25 – 30	Combined sources	6
Helixⁱ	31 – 41	Combined sources	11
Beta strandⁱ	48 – 51	Combined sources	4
Helixⁱ	52 – 54	Combined sources	3
Helixⁱ	56 – 66	Combined sources	11
Beta strandⁱ	67 – 70	Combined sources	4
Beta strandⁱ	73 – 76	Combined sources	4
Beta strandⁱ	79 – 83	Combined sources	5
Helixⁱ	84 – 92	Combined sources	9
Helixⁱ	95 – 107	Combined sources	13
Beta strandⁱ	108 – 110	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	P0AC69
SMRⁱ	P0AC69
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P0AC69

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	5 – 107	GlutaredoxinPROSITE-ProRule annotationAdd BLAST		103

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	84 – 85	Glutathione binding		2

Sequence similaritiesⁱ

Belongs to the glutaredoxin family. Monothiol subfamily.Curated

Keywords - Domainⁱ

Redox-active center

Phylogenomic databases

eggNOGⁱ	ENOG4105M2J Bacteria COG0278 LUCA
HOGENOMⁱ	HOG000095211
InParanoidⁱ	P0AC69
KEGG Orthology (KO) More...KOⁱ	K07390
OMAⁱ	KGTKLMP
PhylomeDBⁱ	P0AC69

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd03028 GRX_PICOT_like, 1 hit
InterProⁱ	View protein in InterPro IPR002109 Glutaredoxin IPR033658 GRX_PICOT-like IPR014434 Monothiol_GRX IPR004480 Monothiol_GRX-rel IPR036249 Thioredoxin-like_sf
PANTHERⁱ	PTHR10293 PTHR10293, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00462 Glutaredoxin, 1 hit
PIRSFⁱ	PIRSF005894 Monothiol_GRX, 1 hit
SUPFAMⁱ	SSF52833 SSF52833, 1 hit
TIGRFAMsⁱ	TIGR00365 TIGR00365, 1 hit
PROSITEⁱ	View protein in PROSITE PS51354 GLUTAREDOXIN_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0AC69-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSTTIEKIQR QIAENPILLY MKGSPKLPSC GFSAQAVQAL AACGERFAYV 
        60         70         80         90        100
DILQNPDIRA ELPKYANWPT FPQLWVDGEL VGGCDIVIEM YQRGELQQLI 
       110 
KETAAKYKSE EPDAE

Length:115

Mass (Da):12,879

Last modified:November 8, 2005 - v1

Checksum:ⁱ254B540430632645

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00096 Genomic DNA Translation: AAC74726.1 AP009048 Genomic DNA Translation: BAA15420.1 L01622 Genomic DNA Translation: AAC37010.1
PIRⁱ	H64922
NCBI Reference Sequences More...RefSeqⁱ	NP_416171.1, NC_000913.3 WP_000108172.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC74726; AAC74726; b1654 BAA15420; BAA15420; BAA15420
GeneIDⁱ	946169
KEGGⁱ	ecj:JW1646 eco:b1654
PATRICⁱ	fig\|1411691.4.peg.605

Protein	Similar proteins		Species	Score	Length	Source
P0AC69	Glutaredoxin 4		ECO57		115	UniRef100_P0AC71
Glutaredoxin 4		ECOL6		115
Glutaredoxin 4		SHIFL		115
Glutaredoxin		Escherichia coli NCCP15648		115
Glutaredoxin		Escherichia coli O145 str. RM9872		115
+201

Protein	Similar proteins		Species	Score	Length	Source
P0AC69	Glutaredoxin 4		ECO57		115	UniRef90_P0AC71
Glutaredoxin 4		ECOL6		115
Glutaredoxin 4		SHIFL		115
Glutaredoxin		Escherichia coli NCCP15648		115
Glutaredoxin		Escherichia coli O145 str. RM9872		115
+539

Protein	Similar proteins		Species	Score	Length	Source
P0AC69	Glutaredoxin 4		HAEIN		107	UniRef50_P45085
Glutaredoxin 4		HAEI8		107
Probable monothiol glutaredoxin GrlA		LEGPH		89
Glutaredoxin 4		BUCAI		108
Glutaredoxin 4		ECO57		115
+3344

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00096 Genomic DNA Translation: AAC74726.1 AP009048 Genomic DNA Translation: BAA15420.1 L01622 Genomic DNA Translation: AAC37010.1
PIRⁱ	H64922
RefSeqⁱ	NP_416171.1, NC_000913.3 WP_000108172.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1YKA	NMR	-	A	1-115	[»]
	2WCI	X-ray	1.90	A/B	1-115	[»]
ProteinModelPortalⁱ	P0AC69
SMRⁱ	P0AC69
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4260269, 437 interactors
DIPⁱ	DIP-11729N
IntActⁱ	P0AC69, 18 interactors
STRINGⁱ	316385.ECDH10B_1788

2D gel databases

SWISS-2DPAGEⁱ	P0AC69

SWISS-2DPAGEⁱ

P0AC69

Proteomic databases

EPDⁱ	P0AC69
PaxDbⁱ	P0AC69
PRIDEⁱ	P0AC69

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC74726; AAC74726; b1654 BAA15420; BAA15420; BAA15420
GeneIDⁱ	946169
KEGGⁱ	ecj:JW1646 eco:b1654
PATRICⁱ	fig\|1411691.4.peg.605

Organism-specific databases

EchoBASEⁱ	EB2098
EcoGeneⁱ	EG12181 grxD

Phylogenomic databases

eggNOGⁱ	ENOG4105M2J Bacteria COG0278 LUCA
HOGENOMⁱ	HOG000095211
InParanoidⁱ	P0AC69
KOⁱ	K07390
OMAⁱ	KGTKLMP
PhylomeDBⁱ	P0AC69

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG12181-MONOMER

BioCycⁱ

EcoCyc:EG12181-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	P0AC69
Protein Ontology More...PROⁱ	PR:P0AC69

Family and domain databases

CDDⁱ	cd03028 GRX_PICOT_like, 1 hit
InterProⁱ	View protein in InterPro IPR002109 Glutaredoxin IPR033658 GRX_PICOT-like IPR014434 Monothiol_GRX IPR004480 Monothiol_GRX-rel IPR036249 Thioredoxin-like_sf
PANTHERⁱ	PTHR10293 PTHR10293, 1 hit
Pfamⁱ	View protein in Pfam PF00462 Glutaredoxin, 1 hit
PIRSFⁱ	PIRSF005894 Monothiol_GRX, 1 hit
SUPFAMⁱ	SSF52833 SSF52833, 1 hit
TIGRFAMsⁱ	TIGR00365 TIGR00365, 1 hit
PROSITEⁱ	View protein in PROSITE PS51354 GLUTAREDOXIN_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GLRX4_ECOLI
Accessionⁱ	P0AC69Primary (citable) accession number: P0AC69 Secondary accession number(s): P37010, P77424
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
	Last sequence update:	November 8, 2005
	Last modified:	March 28, 2018
	This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0AC69 (GLRX4_ECOLI)

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Glutaredoxin 4

grxD

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

2D gel databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ