UniProtKB - P0AC55 (GLNK_ECOLI)
Protein
Nitrogen regulatory protein P-II 2
Gene
glnK
Organism
Escherichia coli (strain K12)
Status
Functioni
P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of GlnA. When P-II is uridylylated to P-II-UMP, these events are reversed. When the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP, which causes the deadenylation of glutamine synthetase by GlnE, so activating the enzyme (By similarity).By similarity
GO - Molecular functioni
- ATP binding Source: GO_Central
- enzyme regulator activity Source: GO_Central
- identical protein binding Source: IntAct
GO - Biological processi
- positive regulation of nitrogen utilization Source: EcoCyc
- regulation of nitrogen utilization Source: GO_Central
Keywordsi
Biological process | Transcription, Transcription regulation |
Ligand | Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:PROTEIN-PII2 MetaCyc:PROTEIN-PII2 |
Names & Taxonomyi
Protein namesi | Recommended name: Nitrogen regulatory protein P-II 2 |
Gene namesi | Name:glnK Synonyms:ybaI Ordered Locus Names:b0450, JW0440 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000139772 | 1 – 112 | Nitrogen regulatory protein P-II 2Add BLAST | 112 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 51 | O-UMP-tyrosinePROSITE-ProRule annotation | 1 |
Post-translational modificationi
Uridylylated/deuridylylated by GlnD.
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P0AC55 |
PaxDbi | P0AC55 |
PRIDEi | P0AC55 |
2D gel databases
SWISS-2DPAGEi | P0AC55 |
Interactioni
Subunit structurei
Homotrimer.
Binary interactionsi
Hide detailsP0AC55
With | #Exp. | IntAct |
---|---|---|
amtB [P69681] | 3 | EBI-559503,EBI-9137905 |
glnB [P0A9Z1] | 3 | EBI-559503,EBI-551053 |
glnD [P27249] | 3 | EBI-559503,EBI-552032 |
itself | 3 | EBI-559503,EBI-559503 |
glnL [P0AFB5] | 5 | EBI-559503,EBI-701156 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4263149, 10 interactors 849476, 6 interactors |
DIPi | DIP-35006N |
IntActi | P0AC55, 14 interactors |
STRINGi | 511145.b0450 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AC55 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AC55 |
Family & Domainsi
Sequence similaritiesi
Belongs to the P(II) protein family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | COG0347, Bacteria |
HOGENOMi | CLU_082268_0_0_6 |
InParanoidi | P0AC55 |
PhylomeDBi | P0AC55 |
Family and domain databases
Gene3Di | 3.30.70.120, 1 hit |
InterProi | View protein in InterPro IPR002187, N-reg_PII IPR011322, N-reg_PII-like_a/b IPR015867, N-reg_PII/ATP_PRibTrfase_C IPR017918, N-reg_PII_CS IPR002332, N-reg_PII_urydylation_site |
PANTHERi | PTHR30115, PTHR30115, 1 hit |
Pfami | View protein in Pfam PF00543, P-II, 1 hit |
PIRSFi | PIRSF039144, GlnB, 1 hit |
PRINTSi | PR00340, PIIGLNB |
SMARTi | View protein in SMART SM00938, P-II, 1 hit |
SUPFAMi | SSF54913, SSF54913, 1 hit |
PROSITEi | View protein in PROSITE PS00638, PII_GLNB_CTER, 1 hit PS51343, PII_GLNB_DOM, 1 hit PS00496, PII_GLNB_UMP, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AC55-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLVTVIIKP FKLEDVREAL SSIGIQGLTV TEVKGFGRQK GHAELYRGAE
60 70 80 90 100
YSVNFLPKVK IDVAIADDQL DEVIDIVSKA AYTGKIGDGK IFVAELQRVI
110
RIRTGEADEA AL
Sequence cautioni
The sequence AAB40206 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U40429 Genomic DNA Translation: AAD14836.1 S79842 Genomic DNA Translation: AAA87955.1 U82664 Genomic DNA Translation: AAB40206.1 Different initiation. U00096 Genomic DNA Translation: AAC73553.1 AP009048 Genomic DNA Translation: BAE76230.1 |
PIRi | B64775 |
RefSeqi | NP_414984.1, NC_000913.3 WP_000780338.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73553; AAC73553; b0450 BAE76230; BAE76230; BAE76230 |
GeneIDi | 58463333 945087 |
KEGGi | ecj:JW0440 eco:b0450 |
PATRICi | fig|1411691.4.peg.1825 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U40429 Genomic DNA Translation: AAD14836.1 S79842 Genomic DNA Translation: AAA87955.1 U82664 Genomic DNA Translation: AAB40206.1 Different initiation. U00096 Genomic DNA Translation: AAC73553.1 AP009048 Genomic DNA Translation: BAE76230.1 |
PIRi | B64775 |
RefSeqi | NP_414984.1, NC_000913.3 WP_000780338.1, NZ_STEB01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GNK | X-ray | 2.00 | A/B | 1-112 | [»] | |
2GNK | X-ray | 2.00 | A | 1-112 | [»] | |
2NS1 | X-ray | 1.96 | B | 1-112 | [»] | |
2NUU | X-ray | 2.50 | G/H/I/J/K/L | 1-112 | [»] | |
SMRi | P0AC55 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263149, 10 interactors 849476, 6 interactors |
DIPi | DIP-35006N |
IntActi | P0AC55, 14 interactors |
STRINGi | 511145.b0450 |
2D gel databases
SWISS-2DPAGEi | P0AC55 |
Proteomic databases
jPOSTi | P0AC55 |
PaxDbi | P0AC55 |
PRIDEi | P0AC55 |
Genome annotation databases
EnsemblBacteriai | AAC73553; AAC73553; b0450 BAE76230; BAE76230; BAE76230 |
GeneIDi | 58463333 945087 |
KEGGi | ecj:JW0440 eco:b0450 |
PATRICi | fig|1411691.4.peg.1825 |
Organism-specific databases
EchoBASEi | EB2108 |
Phylogenomic databases
eggNOGi | COG0347, Bacteria |
HOGENOMi | CLU_082268_0_0_6 |
InParanoidi | P0AC55 |
PhylomeDBi | P0AC55 |
Enzyme and pathway databases
BioCyci | EcoCyc:PROTEIN-PII2 MetaCyc:PROTEIN-PII2 |
Miscellaneous databases
EvolutionaryTracei | P0AC55 |
PROi | PR:P0AC55 |
Family and domain databases
Gene3Di | 3.30.70.120, 1 hit |
InterProi | View protein in InterPro IPR002187, N-reg_PII IPR011322, N-reg_PII-like_a/b IPR015867, N-reg_PII/ATP_PRibTrfase_C IPR017918, N-reg_PII_CS IPR002332, N-reg_PII_urydylation_site |
PANTHERi | PTHR30115, PTHR30115, 1 hit |
Pfami | View protein in Pfam PF00543, P-II, 1 hit |
PIRSFi | PIRSF039144, GlnB, 1 hit |
PRINTSi | PR00340, PIIGLNB |
SMARTi | View protein in SMART SM00938, P-II, 1 hit |
SUPFAMi | SSF54913, SSF54913, 1 hit |
PROSITEi | View protein in PROSITE PS00638, PII_GLNB_CTER, 1 hit PS51343, PII_GLNB_DOM, 1 hit PS00496, PII_GLNB_UMP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLNK_ECOLI | |
Accessioni | P0AC55Primary (citable) accession number: P0AC55 Secondary accession number(s): P38504, P77118, Q2MBX6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | April 7, 2021 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families