UniProtKB - P0AC55 (GLNK_ECOLI)

BLAST

>sp|P0AC55|GLNK_ECOLI Nitrogen regulatory protein P-II 2 OS=Escherichia coli (strain K12) OX=83333 GN=glnK PE=1 SV=1
MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAELYRGAEYSVNFLPKVK
IDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRIRTGEADEAAL

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History
Entry version 106 (07 Nov 2018)
Sequence version 1 (08 Nov 2005)
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Protein

Nitrogen regulatory protein P-II 2

Gene

glnK

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of GlnA. When P-II is uridylylated to P-II-UMP, these events are reversed. When the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP, which causes the deadenylation of glutamine synthetase by GlnE, so activating the enzyme (By similarity).By similarity

GO - Molecular functionⁱ

enzyme regulator activity Source: InterPro
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 15150219
- 24561554
nucleotide binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

positive regulation of nitrogen utilization
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 12366843

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Transcription, Transcription regulation
Ligand	Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:PROTEIN-PII2 MetaCyc:PROTEIN-PII2

BioCycⁱ

EcoCyc:PROTEIN-PII2
MetaCyc:PROTEIN-PII2

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Nitrogen regulatory protein P-II 2
Gene namesⁱ	Name:glnK Synonyms:ybaI Ordered Locus Names:b0450, JW0440
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG12191 glnK

EcoGeneⁱ

EG12191 glnK

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000139772	1 – 112	Nitrogen regulatory protein P-II 2Add BLAST		112

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	51	O-UMP-tyrosinePROSITE-ProRule annotation		1

Post-translational modificationⁱ

Uridylylated/deuridylylated by GlnD.

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	P0AC55
PRIDEⁱ	P0AC55

2D gel databases

SWISS-2DPAGEⁱ	P0AC55

SWISS-2DPAGEⁱ

P0AC55

Interactionⁱ

Subunit structureⁱ

Homotrimer.

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
itself		3	EBI-559503,EBI-559503
amtB	P69681	3	EBI-559503,EBI-9137905
glnB	P0A9Z1	3	EBI-559503,EBI-551053
glnD	P27249	3	EBI-559503,EBI-552032
glnL	P0AFB5	5	EBI-559503,EBI-701156

GO - Molecular functionⁱ

identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 15150219
- 24561554

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	4263149, 10 interactors
Database of interacting proteins More...DIPⁱ	DIP-35006N
IntActⁱ	P0AC55, 14 interactors
STRINGⁱ	316385.ECDH10B_0406

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	1 – 8	Combined sources	8
Helixⁱ	10 – 12	Combined sources	3
Helixⁱ	13 – 22	Combined sources	10
Beta strandⁱ	29 – 35	Combined sources	7
Beta strandⁱ	37 – 39	Combined sources	3
Beta strandⁱ	44 – 46	Combined sources	3
Beta strandⁱ	49 – 51	Combined sources	3
Beta strandⁱ	56 – 66	Combined sources	11
Helixⁱ	67 – 69	Combined sources	3
Helixⁱ	70 – 81	Combined sources	12
Beta strandⁱ	84 – 86	Combined sources	3
Beta strandⁱ	90 – 97	Combined sources	8
Turnⁱ	102 – 104	Combined sources	3
Helixⁱ	108 – 111	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	P0AC55
SMRⁱ	P0AC55
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P0AC55

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the P(II) protein family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4108YZA Bacteria COG0347 LUCA
HOGENOMⁱ	HOG000017847
InParanoidⁱ	P0AC55
KEGG Orthology (KO) More...KOⁱ	K04752
PhylomeDBⁱ	P0AC55

Family and domain databases

Gene3Dⁱ	3.30.70.120, 1 hit
InterProⁱ	View protein in InterPro IPR002187 N-reg_PII IPR011322 N-reg_PII-like_a/b IPR015867 N-reg_PII/ATP_PRibTrfase_C IPR017918 N-reg_PII_CS IPR002332 N-reg_PII_urydylation_site
PANTHERⁱ	PTHR30115 PTHR30115, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00543 P-II, 1 hit
PIRSFⁱ	PIRSF039144 GlnB, 1 hit
PRINTSⁱ	PR00340 PIIGLNB
SMARTⁱ	View protein in SMART SM00938 P-II, 1 hit
SUPFAMⁱ	SSF54913 SSF54913, 1 hit
PROSITEⁱ	View protein in PROSITE PS00638 PII_GLNB_CTER, 1 hit PS51343 PII_GLNB_DOM, 1 hit PS00496 PII_GLNB_UMP, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0AC55-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKLVTVIIKP FKLEDVREAL SSIGIQGLTV TEVKGFGRQK GHAELYRGAE 
        60         70         80         90        100
YSVNFLPKVK IDVAIADDQL DEVIDIVSKA AYTGKIGDGK IFVAELQRVI 
       110 
RIRTGEADEA AL

Length:112

Mass (Da):12,259

Last modified:November 8, 2005 - v1

Checksum:ⁱ1875CD92C162B537

Sequence cautionⁱ

The sequence AAB40206 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U40429 Genomic DNA Translation: AAD14836.1 S79842 Genomic DNA Translation: AAA87955.1 U82664 Genomic DNA Translation: AAB40206.1 Different initiation. U00096 Genomic DNA Translation: AAC73553.1 AP009048 Genomic DNA Translation: BAE76230.1
PIRⁱ	B64775
NCBI Reference Sequences More...RefSeqⁱ	NP_414984.1, NC_000913.3 WP_000780338.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73553; AAC73553; b0450 BAE76230; BAE76230; BAE76230
GeneIDⁱ	945087
KEGGⁱ	ecj:JW0440 eco:b0450
PATRICⁱ	fig\|1411691.4.peg.1825

Protein	Similar proteins		Species	Score	Length	Source
P0AC55	Nitrogen regulatory protein P-II 2		ECO57		112	UniRef100_P0AC57
Nitrogen regulatory protein P-II 2		ECOL6		112
Nitrogen regulatory protein P-II 2		SHIFL		112
Glutamine synthetase		ECOLX		158
Nitrogen regulatory protein P-II 2		Escherichia coli chi7122		158
+196

Protein	Similar proteins		Species	Score	Length	Source
P0AC55	Nitrogen regulatory protein P-II 2		ECO57		112	UniRef90_P0AC57
Nitrogen regulatory protein P-II 2		ECOL6		112
Nitrogen regulatory protein P-II 2		SHIFL		112
Glutamine synthetase		ECOLX		158
Nitrogen regulatory protein P-II 2		Escherichia coli chi7122		158
+208

Protein	Similar proteins		Species	Score	Length	Source
P0AC55	Nitrogen regulatory protein P-II 2		ECO57		112	UniRef50_P0AC57
Nitrogen regulatory protein P-II 2		ECOL6		112
Nitrogen regulatory protein P-II 2		SHIFL		112
Glutamine synthetase		ECOLX		158
Nitrogen regulatory protein P-II 2		Escherichia coli chi7122		158
+354

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U40429 Genomic DNA Translation: AAD14836.1 S79842 Genomic DNA Translation: AAA87955.1 U82664 Genomic DNA Translation: AAB40206.1 Different initiation. U00096 Genomic DNA Translation: AAC73553.1 AP009048 Genomic DNA Translation: BAE76230.1
PIRⁱ	B64775
RefSeqⁱ	NP_414984.1, NC_000913.3 WP_000780338.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1GNK	X-ray	2.00	A/B	1-112	[»]
	2GNK	X-ray	2.00	A	1-112	[»]
	2NS1	X-ray	1.96	B	1-112	[»]
	2NUU	X-ray	2.50	G/H/I/J/K/L	1-112	[»]
ProteinModelPortalⁱ	P0AC55
SMRⁱ	P0AC55
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4263149, 10 interactors
DIPⁱ	DIP-35006N
IntActⁱ	P0AC55, 14 interactors
STRINGⁱ	316385.ECDH10B_0406

2D gel databases

SWISS-2DPAGEⁱ	P0AC55

SWISS-2DPAGEⁱ

P0AC55

Proteomic databases

PaxDbⁱ	P0AC55
PRIDEⁱ	P0AC55

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73553; AAC73553; b0450 BAE76230; BAE76230; BAE76230
GeneIDⁱ	945087
KEGGⁱ	ecj:JW0440 eco:b0450
PATRICⁱ	fig\|1411691.4.peg.1825

Organism-specific databases

EchoBASEⁱ	EB2108
EcoGeneⁱ	EG12191 glnK

Phylogenomic databases

eggNOGⁱ	ENOG4108YZA Bacteria COG0347 LUCA
HOGENOMⁱ	HOG000017847
InParanoidⁱ	P0AC55
KOⁱ	K04752
PhylomeDBⁱ	P0AC55

Enzyme and pathway databases

BioCycⁱ	EcoCyc:PROTEIN-PII2 MetaCyc:PROTEIN-PII2

BioCycⁱ

EcoCyc:PROTEIN-PII2
MetaCyc:PROTEIN-PII2

Miscellaneous databases

EvolutionaryTraceⁱ	P0AC55
Protein Ontology More...PROⁱ	PR:P0AC55

Family and domain databases

Gene3Dⁱ	3.30.70.120, 1 hit
InterProⁱ	View protein in InterPro IPR002187 N-reg_PII IPR011322 N-reg_PII-like_a/b IPR015867 N-reg_PII/ATP_PRibTrfase_C IPR017918 N-reg_PII_CS IPR002332 N-reg_PII_urydylation_site
PANTHERⁱ	PTHR30115 PTHR30115, 1 hit
Pfamⁱ	View protein in Pfam PF00543 P-II, 1 hit
PIRSFⁱ	PIRSF039144 GlnB, 1 hit
PRINTSⁱ	PR00340 PIIGLNB
SMARTⁱ	View protein in SMART SM00938 P-II, 1 hit
SUPFAMⁱ	SSF54913 SSF54913, 1 hit
PROSITEⁱ	View protein in PROSITE PS00638 PII_GLNB_CTER, 1 hit PS51343 PII_GLNB_DOM, 1 hit PS00496 PII_GLNB_UMP, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GLNK_ECOLI
Accessionⁱ	P0AC55Primary (citable) accession number: P0AC55 Secondary accession number(s): P38504, P77118, Q2MBX6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
	Last sequence update:	November 8, 2005
	Last modified:	November 7, 2018
	This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0AC55 (GLNK_ECOLI)

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Nitrogen regulatory protein P-II 2

glnK

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

2D gel databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ