glnK

Functionⁱ

P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of GlnA. When P-II is uridylylated to P-II-UMP, these events are reversed. When the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP, which causes the deadenylation of glutamine synthetase by GlnE, so activating the enzyme (By similarity).By similarity

GO - Molecular functionⁱ

enzyme regulator activity Source: InterPro
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 15150219
- 24561554
nucleotide binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

positive regulation of nitrogen utilization
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 12366843
regulation of transcription, DNA-templated Source: UniProtKB-KW
transcription, DNA-templated Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Transcription, Transcription regulation
Ligand	Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:PROTEIN-PII2 MetaCyc:PROTEIN-PII2

BioCycⁱ

EcoCyc:PROTEIN-PII2
MetaCyc:PROTEIN-PII2

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Nitrogen regulatory protein P-II 2
Gene namesⁱ	Name:glnK Synonyms:ybaI Ordered Locus Names:b0450, JW0440
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG12191 glnK

EcoGeneⁱ

EG12191 glnK

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000139772	1 – 112	Nitrogen regulatory protein P-II 2Add BLAST		112

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	51	O-UMP-tyrosinePROSITE-ProRule annotation		1

Post-translational modificationⁱ

Uridylylated/deuridylylated by GlnD.

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	P0AC55
PRIDEⁱ	P0AC55

2D gel databases

SWISS-2DPAGEⁱ	P0AC55

SWISS-2DPAGEⁱ

P0AC55

Interactionⁱ

Subunit structureⁱ

Homotrimer.

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
itself		3	EBI-559503,EBI-559503
amtB	P69681	3	EBI-559503,EBI-9137905
glnB	P0A9Z1	3	EBI-559503,EBI-551053
glnD	P27249	3	EBI-559503,EBI-552032
glnL	P0AFB5	5	EBI-559503,EBI-701156

Show more details

GO - Molecular functionⁱ

identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 15150219
- 24561554

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	4263149, 10 interactors
Database of interacting proteins More...DIPⁱ	DIP-35006N
IntActⁱ	P0AC55, 14 interactors
STRINGⁱ	316385.ECDH10B_0406

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	1 – 8	Combined sources	8
Helixⁱ	10 – 12	Combined sources	3
Helixⁱ	13 – 22	Combined sources	10
Beta strandⁱ	29 – 35	Combined sources	7
Beta strandⁱ	37 – 39	Combined sources	3
Beta strandⁱ	44 – 46	Combined sources	3
Beta strandⁱ	49 – 51	Combined sources	3
Beta strandⁱ	56 – 66	Combined sources	11
Helixⁱ	67 – 69	Combined sources	3
Helixⁱ	70 – 81	Combined sources	12
Beta strandⁱ	84 – 86	Combined sources	3
Beta strandⁱ	90 – 97	Combined sources	8
Turnⁱ	102 – 104	Combined sources	3
Helixⁱ	108 – 111	Combined sources	4

3D structure databases

ProteinModelPortalⁱ	P0AC55
SMRⁱ	P0AC55
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P0AC55

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the P(II) protein family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4108YZA Bacteria COG0347 LUCA
HOGENOMⁱ	HOG000017847
InParanoidⁱ	P0AC55
KEGG Orthology (KO) More...KOⁱ	K04752
OMAⁱ	AIIRMER
PhylomeDBⁱ	P0AC55

Family and domain databases

Gene3Dⁱ	3.30.70.120, 1 hit
InterProⁱ	View protein in InterPro IPR002187 N-reg_PII IPR011322 N-reg_PII-like_a/b IPR015867 N-reg_PII/ATP_PRibTrfase_C IPR017918 N-reg_PII_CS IPR002332 N-reg_PII_urydylation_site
PANTHERⁱ	PTHR30115 PTHR30115, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00543 P-II, 1 hit
PIRSFⁱ	PIRSF039144 GlnB, 1 hit
PRINTSⁱ	PR00340 PIIGLNB
SMARTⁱ	View protein in SMART SM00938 P-II, 1 hit
SUPFAMⁱ	SSF54913 SSF54913, 1 hit
PROSITEⁱ	View protein in PROSITE PS00638 PII_GLNB_CTER, 1 hit PS51343 PII_GLNB_DOM, 1 hit PS00496 PII_GLNB_UMP, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0AC55-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKLVTVIIKP FKLEDVREAL SSIGIQGLTV TEVKGFGRQK GHAELYRGAE 
        60         70         80         90        100
YSVNFLPKVK IDVAIADDQL DEVIDIVSKA AYTGKIGDGK IFVAELQRVI 
       110 
RIRTGEADEA AL

Length:112

Mass (Da):12,259

Last modified:November 8, 2005 - v1

Checksum:ⁱ1875CD92C162B537

GO

Sequence cautionⁱ

The sequence AAB40206 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U40429 Genomic DNA Translation: AAD14836.1 S79842 Genomic DNA Translation: AAA87955.1 U82664 Genomic DNA Translation: AAB40206.1 Different initiation. U00096 Genomic DNA Translation: AAC73553.1 AP009048 Genomic DNA Translation: BAE76230.1
PIRⁱ	B64775
NCBI Reference Sequences More...RefSeqⁱ	NP_414984.1, NC_000913.3 WP_000780338.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73553; AAC73553; b0450 BAE76230; BAE76230; BAE76230
GeneIDⁱ	945087
KEGGⁱ	ecj:JW0440 eco:b0450
PATRICⁱ	fig\|1411691.4.peg.1825

Protein	Similar proteins		Species	Score	Length
P0AC55	Nitrogen regulatory protein P-II 2		ECO57		112
Nitrogen regulatory protein P-II 2		ECOL6		112
Nitrogen regulatory protein P-II 2		SHIFL		112
Nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB		Escherichia coli NCCP15648		112
Nitrogen regulatory protein P-II, glnK		Klebsiella pneumoniae IS22		112
+192

Protein	Similar proteins		Species	Score	Length
P0AC55	Nitrogen regulatory protein P-II 2		ECO57		112
Nitrogen regulatory protein P-II 2		ECOL6		112
Nitrogen regulatory protein P-II 2		SHIFL		112
Nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB		Escherichia coli NCCP15648		112
Nitrogen regulatory protein P-II, glnK		Klebsiella pneumoniae IS22		112
+205

Protein	Similar proteins		Species	Score	Length
P0AC55	Nitrogen regulatory protein P-II 2		ECO57		112
Nitrogen regulatory protein P-II 2		ECOL6		112
Nitrogen regulatory protein P-II 2		SHIFL		112
Nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB		Escherichia coli NCCP15648		112
Nitrogen regulatory protein P-II, glnK		Klebsiella pneumoniae IS22		112
+1064

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U40429 Genomic DNA Translation: AAD14836.1 S79842 Genomic DNA Translation: AAA87955.1 U82664 Genomic DNA Translation: AAB40206.1 Different initiation. U00096 Genomic DNA Translation: AAC73553.1 AP009048 Genomic DNA Translation: BAE76230.1
PIRⁱ	B64775
NCBI Reference Sequences More...RefSeqⁱ	NP_414984.1, NC_000913.3 WP_000780338.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1GNK	X-ray	2.00	A/B	1-112	[»]
	2GNK	X-ray	2.00	A	1-112	[»]
	2NS1	X-ray	1.96	B	1-112	[»]
	2NUU	X-ray	2.50	G/H/I/J/K/L	1-112	[»]
ProteinModelPortalⁱ	P0AC55
SMRⁱ	P0AC55
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4263149, 10 interactors
Database of interacting proteins More...DIPⁱ	DIP-35006N
IntActⁱ	P0AC55, 14 interactors
STRINGⁱ	316385.ECDH10B_0406

2D gel databases

SWISS-2DPAGEⁱ	P0AC55

SWISS-2DPAGEⁱ

P0AC55

Proteomic databases

PaxDbⁱ	P0AC55
PRIDEⁱ	P0AC55

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73553; AAC73553; b0450 BAE76230; BAE76230; BAE76230
GeneIDⁱ	945087
KEGGⁱ	ecj:JW0440 eco:b0450
PATRICⁱ	fig\|1411691.4.peg.1825

Organism-specific databases

EchoBASEⁱ	EB2108
EcoGeneⁱ	EG12191 glnK

Phylogenomic databases

eggNOGⁱ	ENOG4108YZA Bacteria COG0347 LUCA
HOGENOMⁱ	HOG000017847
InParanoidⁱ	P0AC55
KEGG Orthology (KO) More...KOⁱ	K04752
OMAⁱ	AIIRMER
PhylomeDBⁱ	P0AC55

Enzyme and pathway databases

BioCycⁱ	EcoCyc:PROTEIN-PII2 MetaCyc:PROTEIN-PII2

BioCycⁱ

EcoCyc:PROTEIN-PII2
MetaCyc:PROTEIN-PII2

Miscellaneous databases

EvolutionaryTraceⁱ	P0AC55
Protein Ontology More...PROⁱ	PR:P0AC55

Family and domain databases

Gene3Dⁱ	3.30.70.120, 1 hit
InterProⁱ	View protein in InterPro IPR002187 N-reg_PII IPR011322 N-reg_PII-like_a/b IPR015867 N-reg_PII/ATP_PRibTrfase_C IPR017918 N-reg_PII_CS IPR002332 N-reg_PII_urydylation_site
PANTHERⁱ	PTHR30115 PTHR30115, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00543 P-II, 1 hit
PIRSFⁱ	PIRSF039144 GlnB, 1 hit
PRINTSⁱ	PR00340 PIIGLNB
SMARTⁱ	View protein in SMART SM00938 P-II, 1 hit
SUPFAMⁱ	SSF54913 SSF54913, 1 hit
PROSITEⁱ	View protein in PROSITE PS00638 PII_GLNB_CTER, 1 hit PS51343 PII_GLNB_DOM, 1 hit PS00496 PII_GLNB_UMP, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GLNK_ECOLI
Accessionⁱ	P0AC55Primary (citable) accession number: P0AC55 Secondary accession number(s): P38504, P77118, Q2MBX6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
	Last sequence update:	November 8, 2005
	Last modified:	March 28, 2018
	This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families

UniProtKB

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Supporting data

UniProtKB - P0AC55 (GLNK_ECOLI)

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Nitrogen regulatory protein P-II 2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

2D gel databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ