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Protein

Nitrogen regulatory protein P-II 2

Gene

glnK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of GlnA. When P-II is uridylylated to P-II-UMP, these events are reversed. When the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP, which causes the deadenylation of glutamine synthetase by GlnE, so activating the enzyme (By similarity).By similarity

GO - Molecular functioni

  • enzyme regulator activity Source: InterPro
  • identical protein binding Source: IntAct
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • positive regulation of nitrogen utilization Source: EcoCyc

Keywordsi

Biological processTranscription, Transcription regulation
LigandNucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PROTEIN-PII2
MetaCyc:PROTEIN-PII2

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulatory protein P-II 2
Gene namesi
Name:glnK
Synonyms:ybaI
Ordered Locus Names:b0450, JW0440
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12191 glnK

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001397721 – 112Nitrogen regulatory protein P-II 2Add BLAST112

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei51O-UMP-tyrosinePROSITE-ProRule annotation1

Post-translational modificationi

Uridylylated/deuridylylated by GlnD.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AC55
PRIDEiP0AC55

2D gel databases

SWISS-2DPAGEiP0AC55

Interactioni

Subunit structurei

Homotrimer.

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4263149, 10 interactors
DIPiDIP-35006N
IntActiP0AC55, 14 interactors
STRINGi316385.ECDH10B_0406

Structurei

Secondary structure

1112
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0AC55
SMRiP0AC55
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AC55

Family & Domainsi

Sequence similaritiesi

Belongs to the P(II) protein family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108YZA Bacteria
COG0347 LUCA
HOGENOMiHOG000017847
InParanoidiP0AC55
KOiK04752
PhylomeDBiP0AC55

Family and domain databases

Gene3Di3.30.70.120, 1 hit
InterProiView protein in InterPro
IPR002187 N-reg_PII
IPR011322 N-reg_PII-like_a/b
IPR015867 N-reg_PII/ATP_PRibTrfase_C
IPR017918 N-reg_PII_CS
IPR002332 N-reg_PII_urydylation_site
PANTHERiPTHR30115 PTHR30115, 1 hit
PfamiView protein in Pfam
PF00543 P-II, 1 hit
PIRSFiPIRSF039144 GlnB, 1 hit
PRINTSiPR00340 PIIGLNB
SMARTiView protein in SMART
SM00938 P-II, 1 hit
SUPFAMiSSF54913 SSF54913, 1 hit
PROSITEiView protein in PROSITE
PS00638 PII_GLNB_CTER, 1 hit
PS51343 PII_GLNB_DOM, 1 hit
PS00496 PII_GLNB_UMP, 1 hit

Sequencei

Sequence statusi: Complete.

P0AC55-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLVTVIIKP FKLEDVREAL SSIGIQGLTV TEVKGFGRQK GHAELYRGAE
60 70 80 90 100
YSVNFLPKVK IDVAIADDQL DEVIDIVSKA AYTGKIGDGK IFVAELQRVI
110
RIRTGEADEA AL
Length:112
Mass (Da):12,259
Last modified:November 8, 2005 - v1
Checksum:i1875CD92C162B537
GO

Sequence cautioni

The sequence AAB40206 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40429 Genomic DNA Translation: AAD14836.1
S79842 Genomic DNA Translation: AAA87955.1
U82664 Genomic DNA Translation: AAB40206.1 Different initiation.
U00096 Genomic DNA Translation: AAC73553.1
AP009048 Genomic DNA Translation: BAE76230.1
PIRiB64775
RefSeqiNP_414984.1, NC_000913.3
WP_000780338.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73553; AAC73553; b0450
BAE76230; BAE76230; BAE76230
GeneIDi945087
KEGGiecj:JW0440
eco:b0450
PATRICifig|1411691.4.peg.1825

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40429 Genomic DNA Translation: AAD14836.1
S79842 Genomic DNA Translation: AAA87955.1
U82664 Genomic DNA Translation: AAB40206.1 Different initiation.
U00096 Genomic DNA Translation: AAC73553.1
AP009048 Genomic DNA Translation: BAE76230.1
PIRiB64775
RefSeqiNP_414984.1, NC_000913.3
WP_000780338.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GNKX-ray2.00A/B1-112[»]
2GNKX-ray2.00A1-112[»]
2NS1X-ray1.96B1-112[»]
2NUUX-ray2.50G/H/I/J/K/L1-112[»]
ProteinModelPortaliP0AC55
SMRiP0AC55
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263149, 10 interactors
DIPiDIP-35006N
IntActiP0AC55, 14 interactors
STRINGi316385.ECDH10B_0406

2D gel databases

SWISS-2DPAGEiP0AC55

Proteomic databases

PaxDbiP0AC55
PRIDEiP0AC55

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73553; AAC73553; b0450
BAE76230; BAE76230; BAE76230
GeneIDi945087
KEGGiecj:JW0440
eco:b0450
PATRICifig|1411691.4.peg.1825

Organism-specific databases

EchoBASEiEB2108
EcoGeneiEG12191 glnK

Phylogenomic databases

eggNOGiENOG4108YZA Bacteria
COG0347 LUCA
HOGENOMiHOG000017847
InParanoidiP0AC55
KOiK04752
PhylomeDBiP0AC55

Enzyme and pathway databases

BioCyciEcoCyc:PROTEIN-PII2
MetaCyc:PROTEIN-PII2

Miscellaneous databases

EvolutionaryTraceiP0AC55
PROiPR:P0AC55

Family and domain databases

Gene3Di3.30.70.120, 1 hit
InterProiView protein in InterPro
IPR002187 N-reg_PII
IPR011322 N-reg_PII-like_a/b
IPR015867 N-reg_PII/ATP_PRibTrfase_C
IPR017918 N-reg_PII_CS
IPR002332 N-reg_PII_urydylation_site
PANTHERiPTHR30115 PTHR30115, 1 hit
PfamiView protein in Pfam
PF00543 P-II, 1 hit
PIRSFiPIRSF039144 GlnB, 1 hit
PRINTSiPR00340 PIIGLNB
SMARTiView protein in SMART
SM00938 P-II, 1 hit
SUPFAMiSSF54913 SSF54913, 1 hit
PROSITEiView protein in PROSITE
PS00638 PII_GLNB_CTER, 1 hit
PS51343 PII_GLNB_DOM, 1 hit
PS00496 PII_GLNB_UMP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGLNK_ECOLI
AccessioniPrimary (citable) accession number: P0AC55
Secondary accession number(s): P38504, P77118, Q2MBX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: November 7, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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