UniProtKB - P0AC47 (FRDB_ECOLI)
Protein
Fumarate reductase iron-sulfur subunit
Gene
frdB
Organism
Escherichia coli (strain K12)
Status
Functioni
Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; fumarate reductase is used during anaerobic growth, and succinate dehydrogenase is used during aerobic growth. The QFR enzyme complex binds 2 quinones in or near the membrane; 1 near the [3Fe-4S] cluster (QP is proximal to the [3Fe-4S] cluster, on the cytoplasmic side of the membrane) while QD (the distal cluster) is on the other side of the membrane. It is not clear if both of the quinol-binding sites are functionally relevant (PubMed:10373108, PubMed:11850430).2 Publications
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] cluster1 PublicationNote: Binds 1 [2Fe-2S] cluster.1 Publication
- [3Fe-4S] cluster1 PublicationNote: Binds 1 [3Fe-4S] cluster.1 Publication
- [4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 14 | MenaquinoneCombined sources1 Publication | 1 | |
Metal bindingi | 58 | Iron-sulfur 1 (2Fe-2S)Combined sources1 Publication | 1 | |
Metal bindingi | 63 | Iron-sulfur 1 (2Fe-2S)Combined sources1 Publication | 1 | |
Metal bindingi | 66 | Iron-sulfur 1 (2Fe-2S)Combined sources1 Publication | 1 | |
Metal bindingi | 78 | Iron-sulfur 1 (2Fe-2S)Combined sources1 Publication | 1 | |
Metal bindingi | 149 | Iron-sulfur 2 (4Fe-4S)Combined sources1 Publication | 1 | |
Metal bindingi | 152 | Iron-sulfur 2 (4Fe-4S)Combined sources1 Publication | 1 | |
Metal bindingi | 155 | Iron-sulfur 2 (4Fe-4S)Combined sources1 Publication | 1 | |
Metal bindingi | 159 | Iron-sulfur 3 (3Fe-4S)Combined sources1 Publication | 1 | |
Metal bindingi | 205 | Iron-sulfur 3 (3Fe-4S)Combined sources1 Publication | 1 | |
Metal bindingi | 211 | Iron-sulfur 3 (3Fe-4S)Combined sources1 Publication | 1 | |
Metal bindingi | 215 | Iron-sulfur 2 (4Fe-4S)Combined sources1 Publication | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: EcoCyc
- 3 iron, 4 sulfur cluster binding Source: EcoCyc
- 4 iron, 4 sulfur cluster binding Source: EcoCyc
- electron transfer activity Source: EcoCyc
- fumarate reductase (menaquinone) Source: UniProtKB-EC
- iron-sulfur cluster binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- anaerobic respiration Source: EcoCyc
- bacterial-type flagellum assembly Source: EcoCyc
- bacterial-type flagellum-dependent cell motility Source: EcoCyc
- fermentation Source: EcoCyc
- tricarboxylic acid cycle Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Transport, Tricarboxylic acid cycle |
Ligand | 2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:FUM-FE-S MetaCyc:FUM-FE-S |
Names & Taxonomyi
Protein namesi | Recommended name: Fumarate reductase iron-sulfur subunit (EC:1.3.5.4)Alternative name(s): Quinol-fumarate reductase iron-sulfur subunit1 Publication Short name: QFR iron-sulfur subunit1 Publication |
Gene namesi | Name:frdB Ordered Locus Names:b4153, JW4114 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Cytosol
- cytosol Source: EcoCyc
Plasma Membrane
- plasma membrane fumarate reductase complex Source: EcoCyc
Other locations
- membrane Source: UniProtKB
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 58 | C → S: Affects center 1 (2Fe-2S). 1 Publication | 1 | |
Mutagenesisi | 63 | C → S: Affects center 1 (2Fe-2S). 1 Publication | 1 | |
Mutagenesisi | 66 | C → S: Affects center 1 (2Fe-2S). 1 Publication | 1 | |
Mutagenesisi | 78 | C → S: Affects center 1 (2Fe-2S). 1 Publication | 1 |
Chemistry databases
DrugBanki | DB07490, 2-[1-(4-CHLORO-PHENYL)-ETHYL]-4,6-DINITRO-PHENOL DB07918, 2-heptyl-4-hydroxyquinoline N-oxide |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000158698 | 2 – 244 | Fumarate reductase iron-sulfur subunitAdd BLAST | 243 |
Proteomic databases
jPOSTi | P0AC47 |
PaxDbi | P0AC47 |
PRIDEi | P0AC47 |
Interactioni
Subunit structurei
Part of an enzyme complex containing four subunits: a flavoprotein (FrdA), an iron-sulfur protein (FrdB), and two hydrophobic anchor proteins (FrdC and FrdD).
2 PublicationsBinary interactionsi
Hide detailsP0AC47
With | #Exp. | IntAct |
---|---|---|
frdA [P00363] | 4 | EBI-906724,EBI-550480 |
mutT [P08337] | 4 | EBI-906724,EBI-1121389 |
ompW [P0A915] | 2 | EBI-906724,EBI-1132929 |
Protein-protein interaction databases
BioGRIDi | 4262701, 452 interactors 852958, 2 interactors |
ComplexPortali | CPX-1967, Plasma membrane fumarate reductase complex |
DIPi | DIP-9682N |
IntActi | P0AC47, 7 interactors |
STRINGi | 511145.b4153 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AC47 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AC47 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 16 – 97 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 82 | |
Domaini | 140 – 169 | 4Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 30 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 226 – 229 | Menaquinone bindingCombined sources1 Publication | 4 |
Sequence similaritiesi
Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.Curated
Phylogenomic databases
eggNOGi | COG0479, Bacteria |
HOGENOMi | CLU_044838_3_2_6 |
InParanoidi | P0AC47 |
PhylomeDBi | P0AC47 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
Gene3Di | 1.10.1060.10, 1 hit 3.10.20.30, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006058, 2Fe2S_fd_BS IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR012675, Beta-grasp_dom_sf IPR009051, Helical_ferredxn IPR004489, Succ_DH/fum_Rdtase_Fe-S IPR025192, Succ_DH/fum_Rdtase_N |
Pfami | View protein in Pfam PF13085, Fer2_3, 1 hit PF13183, Fer4_8, 1 hit |
SUPFAMi | SSF46548, SSF46548, 1 hit SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR00384, dhsB, 1 hit |
PROSITEi | View protein in PROSITE PS00197, 2FE2S_FER_1, 1 hit PS51085, 2FE2S_FER_2, 1 hit PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AC47-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEMKNLKIE VVRYNPEVDT APHSAFYEVP YDATTSLLDA LGYIKDNLAP
60 70 80 90 100
DLSYRWSCRM AICGSCGMMV NNVPKLACKT FLRDYTDGMK VEALANFPIE
110 120 130 140 150
RDLVVDMTHF IESLEAIKPY IIGNSRTADQ GTNIQTPAQM AKYHQFSGCI
160 170 180 190 200
NCGLCYAACP QFGLNPEFIG PAAITLAHRY NEDSRDHGKK ERMAQLNSQN
210 220 230 240
GVWSCTFVGY CSEVCPKHVD PAAAIQQGKV ESSKDFLIAT LKPR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01611 Genomic DNA Translation: AAA23438.1 U14003 Genomic DNA Translation: AAA97052.1 U00096 Genomic DNA Translation: AAC77113.1 AP009048 Genomic DNA Translation: BAE78157.1 V00277 Genomic DNA Translation: CAA23534.1 |
PIRi | A00377, RDECFS |
RefSeqi | NP_418577.1, NC_000913.3 WP_000829498.1, NZ_STEB01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC77113; AAC77113; b4153 BAE78157; BAE78157; BAE78157 |
GeneIDi | 58391485 948666 |
KEGGi | ecj:JW4114 eco:b4153 |
PATRICi | fig|1411691.4.peg.2545 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01611 Genomic DNA Translation: AAA23438.1 U14003 Genomic DNA Translation: AAA97052.1 U00096 Genomic DNA Translation: AAC77113.1 AP009048 Genomic DNA Translation: BAE78157.1 V00277 Genomic DNA Translation: CAA23534.1 |
PIRi | A00377, RDECFS |
RefSeqi | NP_418577.1, NC_000913.3 WP_000829498.1, NZ_STEB01000014.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KF6 | X-ray | 2.70 | B/N | 2-244 | [»] | |
1KFY | X-ray | 3.60 | B/N | 2-244 | [»] | |
1L0V | X-ray | 3.30 | B/N | 2-244 | [»] | |
2B76 | X-ray | 3.30 | B/N | 2-244 | [»] | |
3CIR | X-ray | 3.65 | B/N | 2-244 | [»] | |
3P4P | X-ray | 2.80 | B/N | 2-244 | [»] | |
3P4Q | X-ray | 3.35 | B/N | 2-244 | [»] | |
3P4R | X-ray | 3.05 | B/N | 2-244 | [»] | |
3P4S | X-ray | 3.10 | B/N | 2-244 | [»] | |
SMRi | P0AC47 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262701, 452 interactors 852958, 2 interactors |
ComplexPortali | CPX-1967, Plasma membrane fumarate reductase complex |
DIPi | DIP-9682N |
IntActi | P0AC47, 7 interactors |
STRINGi | 511145.b4153 |
Chemistry databases
DrugBanki | DB07490, 2-[1-(4-CHLORO-PHENYL)-ETHYL]-4,6-DINITRO-PHENOL DB07918, 2-heptyl-4-hydroxyquinoline N-oxide |
Proteomic databases
jPOSTi | P0AC47 |
PaxDbi | P0AC47 |
PRIDEi | P0AC47 |
Genome annotation databases
EnsemblBacteriai | AAC77113; AAC77113; b4153 BAE78157; BAE78157; BAE78157 |
GeneIDi | 58391485 948666 |
KEGGi | ecj:JW4114 eco:b4153 |
PATRICi | fig|1411691.4.peg.2545 |
Organism-specific databases
EchoBASEi | EB0327 |
Phylogenomic databases
eggNOGi | COG0479, Bacteria |
HOGENOMi | CLU_044838_3_2_6 |
InParanoidi | P0AC47 |
PhylomeDBi | P0AC47 |
Enzyme and pathway databases
BioCyci | EcoCyc:FUM-FE-S MetaCyc:FUM-FE-S |
Miscellaneous databases
EvolutionaryTracei | P0AC47 |
PROi | PR:P0AC47 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
Gene3Di | 1.10.1060.10, 1 hit 3.10.20.30, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006058, 2Fe2S_fd_BS IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR012675, Beta-grasp_dom_sf IPR009051, Helical_ferredxn IPR004489, Succ_DH/fum_Rdtase_Fe-S IPR025192, Succ_DH/fum_Rdtase_N |
Pfami | View protein in Pfam PF13085, Fer2_3, 1 hit PF13183, Fer4_8, 1 hit |
SUPFAMi | SSF46548, SSF46548, 1 hit SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR00384, dhsB, 1 hit |
PROSITEi | View protein in PROSITE PS00197, 2FE2S_FER_1, 1 hit PS51085, 2FE2S_FER_2, 1 hit PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FRDB_ECOLI | |
Accessioni | P0AC47Primary (citable) accession number: P0AC47 Secondary accession number(s): P00364, Q2M6E9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 128 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families