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UniProtKB - P0AC30 (FTSX_ECOLI)
Protein
Cell division protein FtsX
Gene
ftsX
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the ABC transporter FtsEX involved in cellular division. Important for assembly or stability of the septal ring. Encoded in an operon consisting of genes ftsY, ftsE and ftsX.
2 PublicationsMiscellaneous
Overexpression leads to strong inhibition of growth, cell filamentation and eventually cell death.
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: EcoliWiki
- positive regulation of cell division Source: ComplexPortal
Keywordsi
Biological process | Cell cycle, Cell division |
Enzyme and pathway databases
BioCyci | EcoCyc:FTSX-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Cell division protein FtsX |
Gene namesi | Name:ftsX Synonyms:ftsS Ordered Locus Names:b3462, JW3427 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 3 Publications; Multi-pass membrane protein 3 Publications
Note: Localizes to the septal ring at the later stages of cell growth and remains there until division is complete. This localization is dependent on localization of FtsZ, FtsA and ZipA, but not on the downstream division proteins FtsK, FtsQ or FtsI.
Cell Wall
- Gram-negative-bacterium-type cell wall Source: UniProtKB
Plasma Membrane
- plasma membrane Source: EcoCyc
- plasma membrane protein complex Source: ComplexPortal
Other locations
- ATPase complex Source: ComplexPortal
- cell division site Source: EcoliWiki
- division septum Source: ComplexPortal
- integral component of membrane Source: EcoliWiki
- membrane Source: ComplexPortal
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 74 | CytoplasmicSequence analysisAdd BLAST | 74 | |
Transmembranei | 75 – 95 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 96 – 223 | PeriplasmicSequence analysisAdd BLAST | 128 | |
Transmembranei | 224 – 244 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 245 – 275 | CytoplasmicSequence analysisAdd BLAST | 31 | |
Transmembranei | 276 – 296 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 297 – 322 | PeriplasmicSequence analysisAdd BLAST | 26 | |
Transmembranei | 323 – 343 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 344 – 352 | CytoplasmicSequence analysis | 9 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Double deletion mutant FtsEX grows well on plates with LB medium with 1% NaCl forming healthy colonies at 30 degrees Celsius, whereas at 37 or 42 degrees Celsius, the colonies are very tiny and sick. In culture medium it exhibits mild elongation of cells at 30 degrees Celsius, and become very extensive at higher temperatures. This double mutant does not grow on LB culture medium without NaCl (LBON) at any temperature, possibly due to excessive lethal filamentation. Supplementation with any of a variety of osmolytes, such as glucose, sucrose, glycerol, or NaCl, completely rescues the growth on LBON medium. Though the growth is completely inhibited on LBON plates, the cultures grown in LBON broth show an initial increase in cell mass before the cessation of growth, and at this stage, the culture is extremely filamentous. The filaments grown in LB at 42 degrees Celsius or in LBON at 30 degrees Celsius are very long and appear to have elongated cells. They are normal and regularly spaced without any significant chromosomal aberrations or partition defects.1 Publication
Chemistry databases
ChEMBLi | CHEMBL3309011 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166792 | 1 – 352 | Cell division protein FtsXAdd BLAST | 352 |
Proteomic databases
jPOSTi | P0AC30 |
PaxDbi | P0AC30 |
PRIDEi | P0AC30 |
Interactioni
Subunit structurei
Forms a membrane-associated complex with FtsE.
Binary interactionsi
P0AC30
With | #Exp. | IntAct |
---|---|---|
envC [P37690] | 2 | EBI-1119111,EBI-15948725 |
Protein-protein interaction databases
BioGRIDi | 4262498, 606 interactors |
ComplexPortali | CPX-4602, FtsEX ABC cell division complex |
DIPi | DIP-35993N |
IntActi | P0AC30, 7 interactors |
STRINGi | 511145.b3462 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AC30 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 19 – 48 | DisorderedSequence analysisAdd BLAST | 30 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2177, Bacteria |
HOGENOMi | CLU_073546_0_0_6 |
InParanoidi | P0AC30 |
PhylomeDBi | P0AC30 |
Family and domain databases
InterProi | View protein in InterPro IPR004513, ABC_transpt_FtsX IPR040690, FtsX_ECD |
PANTHERi | PTHR47755, PTHR47755, 1 hit |
Pfami | View protein in Pfam PF18075, FtsX_ECD, 1 hit |
PIRSFi | PIRSF003097, FtsX, 1 hit |
TIGRFAMsi | TIGR00439, ftsX, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AC30-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNKRDAINHI RQFGGRLDRF RKSVGGSGDG GRNAPKRAKS SPKPVNRKTN
60 70 80 90 100
VFNEQVRYAF HGALQDLKSK PFATFLTVMV IAISLTLPSV CYMVYKNVNQ
110 120 130 140 150
AATQYYPSPQ ITVYLQKTLD DDAAAGVVAQ LQAEQGVEKV NYLSREDALG
160 170 180 190 200
EFRNWSGFGG ALDMLEENPL PAVAVVIPKL DFQGTESLNT LRDRITQING
210 220 230 240 250
IDEVRMDDSW FARLAALTGL VGRVSAMIGV LMVAAVFLVI GNSVRLSIFA
260 270 280 290 300
RRDSINVQKL IGATDGFILR PFLYGGALLG FSGALLSLIL SEILVLRLSS
310 320 330 340 350
AVAEVAQVFG TKFDINGLSF DECLLLLLVC SMIGWVAAWL ATVQHLRHFT
PE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04398 Genomic DNA Translation: CAA27986.1 U00039 Genomic DNA Translation: AAB18437.1 U00096 Genomic DNA Translation: AAC76487.1 AP009048 Genomic DNA Translation: BAE77831.1 |
PIRi | S03132, CEECFX |
RefSeqi | NP_417919.1, NC_000913.3 WP_001042003.1, NZ_STEB01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC76487; AAC76487; b3462 BAE77831; BAE77831; BAE77831 |
GeneIDi | 66672654 947969 |
KEGGi | ecj:JW3427 eco:b3462 |
PATRICi | fig|511145.12.peg.3561 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04398 Genomic DNA Translation: CAA27986.1 U00039 Genomic DNA Translation: AAB18437.1 U00096 Genomic DNA Translation: AAC76487.1 AP009048 Genomic DNA Translation: BAE77831.1 |
PIRi | S03132, CEECFX |
RefSeqi | NP_417919.1, NC_000913.3 WP_001042003.1, NZ_STEB01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6TPI | X-ray | 2.10 | B/C | 110-209 | [»] | |
SMRi | P0AC30 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262498, 606 interactors |
ComplexPortali | CPX-4602, FtsEX ABC cell division complex |
DIPi | DIP-35993N |
IntActi | P0AC30, 7 interactors |
STRINGi | 511145.b3462 |
Chemistry databases
ChEMBLi | CHEMBL3309011 |
Proteomic databases
jPOSTi | P0AC30 |
PaxDbi | P0AC30 |
PRIDEi | P0AC30 |
Genome annotation databases
EnsemblBacteriai | AAC76487; AAC76487; b3462 BAE77831; BAE77831; BAE77831 |
GeneIDi | 66672654 947969 |
KEGGi | ecj:JW3427 eco:b3462 |
PATRICi | fig|511145.12.peg.3561 |
Organism-specific databases
EchoBASEi | EB0341 |
Phylogenomic databases
eggNOGi | COG2177, Bacteria |
HOGENOMi | CLU_073546_0_0_6 |
InParanoidi | P0AC30 |
PhylomeDBi | P0AC30 |
Enzyme and pathway databases
BioCyci | EcoCyc:FTSX-MONOMER |
Miscellaneous databases
PROi | PR:P0AC30 |
Family and domain databases
InterProi | View protein in InterPro IPR004513, ABC_transpt_FtsX IPR040690, FtsX_ECD |
PANTHERi | PTHR47755, PTHR47755, 1 hit |
Pfami | View protein in Pfam PF18075, FtsX_ECD, 1 hit |
PIRSFi | PIRSF003097, FtsX, 1 hit |
TIGRFAMsi | TIGR00439, ftsX, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FTSX_ECOLI | |
Accessioni | P0AC30Primary (citable) accession number: P0AC30 Secondary accession number(s): P10122, Q2M7C5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | February 23, 2022 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families