UniProtKB - P0AC02 (BAMD_ECOLI)
Protein
Outer membrane protein assembly factor BamD
Gene
bamD
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the outer membrane protein assembly complex (Bam), which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Constitutes, with BamA, the core component of the assembly machinery. Probably involved in transient protein interactions. Efficient substrate folding and insertion into the outer membrane requires all 5 subunits (PubMed:20378773, PubMed:21823654, PubMed:27686148). A lateral gate may open between the first and last strands of the BamA beta-barrel that allows substrate to insert into the outer membrane; comparison of the structures of complete and nearly complete Bam complexes show there is considerable movement of all 5 proteins (PubMed:27686148, PubMed:26900875, PubMed:26901871, PubMed:26744406).9 Publications
GO - Biological processi
- Gram-negative-bacterium-type cell outer membrane assembly Source: EcoCyc
- protein insertion into membrane Source: EcoCyc
Enzyme and pathway databases
BioCyci | EcoCyc:G7352-MONOMER |
Protein family/group databases
TCDBi | 1.B.33.1.3, the outer membrane protein insertion porin (bam complex) (ompip) family |
Names & Taxonomyi
Protein namesi | Recommended name: Outer membrane protein assembly factor BamDUniRule annotation |
Gene namesi | Name:bamDUniRule annotation Synonyms:yfiO Ordered Locus Names:b2595, JW2577 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell outer membrane UniRule annotation3 Publications; Lipid-anchor UniRule annotation2 Publications
GO - Cellular componenti
- Bam protein complex Source: EcoCyc
- cell outer membrane Source: EcoCyc
Keywords - Cellular componenti
Cell outer membrane, MembranePathology & Biotechi
Disruption phenotypei
Depletion decreases the density of outer membrane proteins, but does not significantly affect transport of lipopolysaccharides to the outer membrane.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | UniRule annotationAdd BLAST | 19 | |
ChainiPRO_0000036226 | 20 – 245 | Outer membrane protein assembly factor BamDAdd BLAST | 226 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 20 | N-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 20 | S-diacylglycerol cysteine1 Publication | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
jPOSTi | P0AC02 |
PaxDbi | P0AC02 |
PRIDEi | P0AC02 |
Interactioni
Subunit structurei
Part of the Bam complex, which is composed of the outer membrane protein BamA, and four lipoproteins BamB, BamC, BamD and BamE.
Forms a subcomplex with BamC and BamE.
Interacts directly with BamA. The Bam complex has the shape of a hat, with the BamA beta-barrel crown in the outer membrane and the periplasmic brim formed by the BamA POTRA domains and the 4 lipoproteins (PubMed:27686148, PubMed:26900875, PubMed:26901871, PubMed:26744406).
12 PublicationsBinary interactionsi
Hide detailsP0AC02
With | #Exp. | IntAct |
---|---|---|
atoA [P76459] | 2 | EBI-1128087,EBI-1128061 |
bamA [P0A940] | 26 | EBI-1128087,EBI-907371 |
bamC [P0A903] | 4 | EBI-1128087,EBI-1129043 |
bamE [P0A937] | 3 | EBI-1128087,EBI-6391574 |
Protein-protein interaction databases
BioGRIDi | 4260615, 311 interactors 851422, 3 interactors |
ComplexPortali | CPX-1923, BAM complex |
DIPi | DIP-51120N |
IntActi | P0AC02, 6 interactors |
MINTi | P0AC02 |
STRINGi | 511145.b2595 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AC02 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domaini
The N-terminal may interact with various proteins as a chaperone to assist in the folding and insertion of proteins into the outer membrane. The C-terminal region may serve as the link between BamA, BamC and BamE.1 Publication
Sequence similaritiesi
Belongs to the BamD family.UniRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG4105, Bacteria |
HOGENOMi | CLU_065982_0_2_6 |
InParanoidi | P0AC02 |
PhylomeDBi | P0AC02 |
Family and domain databases
CDDi | cd15830, BamD, 1 hit |
Gene3Di | 1.25.40.10, 1 hit |
HAMAPi | MF_00922, OM_assembly_BamD, 1 hit |
InterProi | View protein in InterPro IPR017689, BamD IPR039565, BamD-like IPR011990, TPR-like_helical_dom_sf |
Pfami | View protein in Pfam PF13525, YfiO, 1 hit |
SUPFAMi | SSF48452, SSF48452, 1 hit |
TIGRFAMsi | TIGR03302, OM_YfiO, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AC02-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTRMKYLVAA ATLSLFLAGC SGSKEEVPDN PPNEIYATAQ QKLQDGNWRQ
60 70 80 90 100
AITQLEALDN RYPFGPYSQQ VQLDLIYAYY KNADLPLAQA AIDRFIRLNP
110 120 130 140 150
THPNIDYVMY MRGLTNMALD DSALQGFFGV DRSDRDPQHA RAAFSDFSKL
160 170 180 190 200
VRGYPNSQYT TDATKRLVFL KDRLAKYEYS VAEYYTERGA WVAVVNRVEG
210 220 230 240
MLRDYPDTQA TRDALPLMEN AYRQMQMNAQ AEKVAKIIAA NSSNT
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 59 | D → Y in CAA94434 (Ref. 4) Curated | 1 |
Mass spectrometryi
Molecular mass is 26582 Da. Determined by ESI. With 3 palmitic acid (C16) acyl chains.1 Publication
Molecular mass is 26603 Da. Determined by ESI. With 2 palmitic (C16) and 1 stearic (C18) acid acyl chains.1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75644.1 AP009048 Genomic DNA Translation: BAA16480.1 Z70523 Genomic DNA Translation: CAA94434.1 |
PIRi | F65037 |
RefSeqi | NP_417086.1, NC_000913.3 WP_000197686.1, NZ_STEB01000040.1 |
Genome annotation databases
EnsemblBacteriai | AAC75644; AAC75644; b2595 BAA16480; BAA16480; BAA16480 |
GeneIDi | 58391812 947086 |
KEGGi | ecj:JW2577 eco:b2595 |
PATRICi | fig|1411691.4.peg.4142 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75644.1 AP009048 Genomic DNA Translation: BAA16480.1 Z70523 Genomic DNA Translation: CAA94434.1 |
PIRi | F65037 |
RefSeqi | NP_417086.1, NC_000913.3 WP_000197686.1, NZ_STEB01000040.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2YHC | X-ray | 1.80 | A | 29-245 | [»] | |
3Q5M | X-ray | 2.60 | A | 23-245 | [»] | |
3TGO | X-ray | 2.90 | A/B | 21-245 | [»] | |
5AYW | X-ray | 3.56 | D | 20-245 | [»] | |
5D0O | X-ray | 2.90 | D | 1-245 | [»] | |
5D0Q | X-ray | 3.50 | D/H | 1-245 | [»] | |
5EKQ | X-ray | 3.39 | D | 20-245 | [»] | |
5LJO | electron microscopy | 4.90 | D | 26-243 | [»] | |
6SN9 | electron microscopy | 9.80 | D | 26-243 | [»] | |
6V05 | electron microscopy | 4.10 | D | 1-245 | [»] | |
SMRi | P0AC02 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260615, 311 interactors 851422, 3 interactors |
ComplexPortali | CPX-1923, BAM complex |
DIPi | DIP-51120N |
IntActi | P0AC02, 6 interactors |
MINTi | P0AC02 |
STRINGi | 511145.b2595 |
Protein family/group databases
TCDBi | 1.B.33.1.3, the outer membrane protein insertion porin (bam complex) (ompip) family |
Proteomic databases
jPOSTi | P0AC02 |
PaxDbi | P0AC02 |
PRIDEi | P0AC02 |
Genome annotation databases
EnsemblBacteriai | AAC75644; AAC75644; b2595 BAA16480; BAA16480; BAA16480 |
GeneIDi | 58391812 947086 |
KEGGi | ecj:JW2577 eco:b2595 |
PATRICi | fig|1411691.4.peg.4142 |
Organism-specific databases
EchoBASEi | EB3974 |
Phylogenomic databases
eggNOGi | COG4105, Bacteria |
HOGENOMi | CLU_065982_0_2_6 |
InParanoidi | P0AC02 |
PhylomeDBi | P0AC02 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7352-MONOMER |
Miscellaneous databases
PROi | PR:P0AC02 |
Family and domain databases
CDDi | cd15830, BamD, 1 hit |
Gene3Di | 1.25.40.10, 1 hit |
HAMAPi | MF_00922, OM_assembly_BamD, 1 hit |
InterProi | View protein in InterPro IPR017689, BamD IPR039565, BamD-like IPR011990, TPR-like_helical_dom_sf |
Pfami | View protein in Pfam PF13525, YfiO, 1 hit |
SUPFAMi | SSF48452, SSF48452, 1 hit |
TIGRFAMsi | TIGR03302, OM_YfiO, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BAMD_ECOLI | |
Accessioni | P0AC02Primary (citable) accession number: P0AC02 Secondary accession number(s): P77146, Q47344 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | February 10, 2021 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families