UniProtKB - P0ABZ1 (FLIG_ECOLI)
Protein
Flagellar motor switch protein FliG
Gene
fliG
Organism
Escherichia coli (strain K12)
Status
Functioni
FliG is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 160 | Part of the EHPQR-motif | 1 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- motor activity Source: InterPro
GO - Biological processi
- bacterial-type flagellum assembly Source: EcoCyc
- bacterial-type flagellum-dependent cell motility Source: EcoCyc
- chemotaxis Source: UniProtKB-KW
Keywordsi
Biological process | Chemotaxis, Flagellar rotation |
Enzyme and pathway databases
BioCyci | EcoCyc:FLIG-FLAGELLAR-SWITCH-PROTEIN |
Names & Taxonomyi
Protein namesi | Recommended name: Flagellar motor switch protein FliG |
Gene namesi | Name:fliG Synonyms:fla AII.2, fla BII Ordered Locus Names:b1939, JW1923 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- bacterial-type flagellum basal body Source: UniProtKB-SubCell
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Bacterial flagellum, Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 252 | Q → W: Decreases binding to YcgR, significantly improves motility in a pdeH disruption mutant. 1 Publication | 1 | |
Mutagenesisi | 284 | D → W: Slightly improves motility in a pdeH disruption mutant. 1 Publication | 1 | |
Mutagenesisi | 292 | N → W: Decreases binding to YcgR, significantly improves motility in a pdeH disruption mutant. 1 Publication | 1 | |
Mutagenesisi | 295 | P → W: Decreases binding to YcgR, significantly improves motility in a pdeH disruption mutant. 1 Publication | 1 | |
Mutagenesisi | 298 | L → W: Slightly improves motility in a pdeH disruption mutant. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000184089 | 1 – 331 | Flagellar motor switch protein FliGAdd BLAST | 331 |
Proteomic databases
PaxDbi | P0ABZ1 |
PRIDEi | P0ABZ1 |
Interactioni
Subunit structurei
Forms dimers and other homooligomers.
Interacts with MotA via residues in its C-terminus.
Interacts with flagellar brake protein YcgR.
1 PublicationBinary interactionsi
Hide detailsP0ABZ1
With | #Exp. | IntAct |
---|---|---|
fliF [P25798] | 4 | EBI-1126524,EBI-1126492 |
itself | 6 | EBI-1126524,EBI-1126524 |
fliM [P06974] | 16 | EBI-1126524,EBI-560439 |
ycgR [P76010] | 3 | EBI-1126524,EBI-554507 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4260384, 25 interactors 850805, 7 interactors |
ComplexPortali | CPX-1082, Flagellar Motor Switch Complex, CW variant CPX-1085, Flagellar Motor Switch Complex, CCW variant |
DIPi | DIP-408N |
IntActi | P0ABZ1, 26 interactors |
MINTi | P0ABZ1 |
STRINGi | 511145.b1939 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 125 – 128 | Part of the EHPQR-motif | 4 |
Sequence similaritiesi
Belongs to the FliG family.Curated
Phylogenomic databases
eggNOGi | COG1536, Bacteria |
HOGENOMi | CLU_047835_2_0_6 |
InParanoidi | P0ABZ1 |
PhylomeDBi | P0ABZ1 |
Family and domain databases
InterProi | View protein in InterPro IPR000090, Flg_Motor_Flig IPR023087, Flg_Motor_Flig_C IPR011002, FliG_a-hlx IPR032779, FliG_M IPR028263, FliG_N |
PANTHERi | PTHR30534, PTHR30534, 1 hit |
Pfami | View protein in Pfam PF01706, FliG_C, 1 hit PF14841, FliG_M, 1 hit PF14842, FliG_N, 1 hit |
PIRSFi | PIRSF003161, FliG, 1 hit |
PRINTSi | PR00954, FLGMOTORFLIG |
SUPFAMi | SSF48029, SSF48029, 2 hits |
TIGRFAMsi | TIGR00207, fliG, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ABZ1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNLTGTDKS VILLMTIGED RAAEVFKHLS QREVQTLSAA MANVTQISNK
60 70 80 90 100
QLTDVLAEFE QEAEQFAALN INANDYLRSV LVKALGEERA ASLLEDILET
110 120 130 140 150
RDTASGIETL NFMEPQSAAD LIRDEHPQII ATILVHLKRA QAADILALFD
160 170 180 190 200
ERLRHDVMLR IATFGGVQPA ALAELTEVLN GLLDGQNLKR SKMGGVRTAA
210 220 230 240 250
EIINLMKTQQ EEAVITAVRE FDGELAQKII DEMFLFENLV DVDDRSIQRL
260 270 280 290 300
LQEVDSESLL IALKGAEQPL REKFLRNMSQ RAADILRDDL ANRGPVRLSQ
310 320 330
VENEQKAILL IVRRLAETGE MVIGSGEDTY V
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 159 – 170 | LRIAT…GVQPA → FVSHLWRRAPS in AAA75241 (PubMed:8224881).CuratedAdd BLAST | 12 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13243 Genomic DNA Translation: AAA75241.1 U46011 Genomic DNA Translation: AAB60186.1 U00096 Genomic DNA Translation: AAC75006.1 AP009048 Genomic DNA Translation: BAA15764.1 D89826 Genomic DNA Translation: BAA14030.1 |
PIRi | H64957 |
RefSeqi | NP_416449.1, NC_000913.3 WP_000067950.1, NZ_STEB01000050.1 |
Genome annotation databases
EnsemblBacteriai | AAC75006; AAC75006; b1939 BAA15764; BAA15764; BAA15764 |
GeneIDi | 48136032 946451 |
KEGGi | ecj:JW1923 eco:b1939 |
PATRICi | fig|1411691.4.peg.312 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13243 Genomic DNA Translation: AAA75241.1 U46011 Genomic DNA Translation: AAB60186.1 U00096 Genomic DNA Translation: AAC75006.1 AP009048 Genomic DNA Translation: BAA15764.1 D89826 Genomic DNA Translation: BAA14030.1 |
PIRi | H64957 |
RefSeqi | NP_416449.1, NC_000913.3 WP_000067950.1, NZ_STEB01000050.1 |
3D structure databases
SMRi | P0ABZ1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260384, 25 interactors 850805, 7 interactors |
ComplexPortali | CPX-1082, Flagellar Motor Switch Complex, CW variant CPX-1085, Flagellar Motor Switch Complex, CCW variant |
DIPi | DIP-408N |
IntActi | P0ABZ1, 26 interactors |
MINTi | P0ABZ1 |
STRINGi | 511145.b1939 |
Proteomic databases
PaxDbi | P0ABZ1 |
PRIDEi | P0ABZ1 |
Genome annotation databases
EnsemblBacteriai | AAC75006; AAC75006; b1939 BAA15764; BAA15764; BAA15764 |
GeneIDi | 48136032 946451 |
KEGGi | ecj:JW1923 eco:b1939 |
PATRICi | fig|1411691.4.peg.312 |
Organism-specific databases
EchoBASEi | EB1607 |
Phylogenomic databases
eggNOGi | COG1536, Bacteria |
HOGENOMi | CLU_047835_2_0_6 |
InParanoidi | P0ABZ1 |
PhylomeDBi | P0ABZ1 |
Enzyme and pathway databases
BioCyci | EcoCyc:FLIG-FLAGELLAR-SWITCH-PROTEIN |
Miscellaneous databases
PROi | PR:P0ABZ1 |
Family and domain databases
InterProi | View protein in InterPro IPR000090, Flg_Motor_Flig IPR023087, Flg_Motor_Flig_C IPR011002, FliG_a-hlx IPR032779, FliG_M IPR028263, FliG_N |
PANTHERi | PTHR30534, PTHR30534, 1 hit |
Pfami | View protein in Pfam PF01706, FliG_C, 1 hit PF14841, FliG_M, 1 hit PF14842, FliG_N, 1 hit |
PIRSFi | PIRSF003161, FliG, 1 hit |
PRINTSi | PR00954, FLGMOTORFLIG |
SUPFAMi | SSF48029, SSF48029, 2 hits |
TIGRFAMsi | TIGR00207, fliG, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FLIG_ECOLI | |
Accessioni | P0ABZ1Primary (citable) accession number: P0ABZ1 Secondary accession number(s): P31067, P76915 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | October 25, 2005 | |
Last modified: | December 2, 2020 | |
This is version 113 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families