UniProtKB - P0ABV6 (TOLR_ECOLI)
Protein
Tol-Pal system protein TolR
Gene
tolR
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity (PubMed:1683466, PubMed:17233825). Required, with TolQ, for the proton motive force-dependent activation of TolA and for TolA-Pal interaction (PubMed:11722743). The Tol-Pal system is also required for polar localization of chemoreceptors clusters (PubMed:24720726). The system also appears to be required for the activity of several outer membrane-localized enzymes with cell wall remodeling activity (PubMed:32152098). Modeling suggests that non-covalent binding of OmpA (from the outer membrane) and TolR (from the inner membrane) to peptidoglycan maintains the position of the cell wall in the periplasm, holding it approximately equidistant from both the inner and outer membranes. Trimeric Lpp controls the width of the periplasm, adjusts its tilt angle to accommodate to the available space, and can compensate in part for an absence of OmpA (Probable).1 Publication5 Publications
(Microbial infection) Involved in the uptake of group A colicins (colicins A, E1, E2, E3, and K) and in the uptake of filamentous phage DNA.2 Publications
Miscellaneous
There are about 900 TolR molecules per cell.1 Publication
GO - Molecular functioni
- transmembrane transporter activity Source: InterPro
GO - Biological processi
- bacteriocin transport Source: EcoliWiki
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-UniRule
- protein transport Source: InterPro
Keywordsi
Biological process | Cell cycle, Cell division |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11011-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Tol-Pal system protein TolRUniRule annotationCurated |
Gene namesi | Name:tolR1 PublicationUniRule annotation Ordered Locus Names:b0738, JW0728 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane UniRule annotation2 Publications; Single-pass membrane protein UniRule annotation1 Publication1 Publication Note: Accumulates at cell constriction sites. Recruitment to the division site is dependent on FtsN activity.1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 17 | Cytoplasmic2 PublicationsAdd BLAST | 17 | |
Transmembranei | 18 – 38 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 39 – 142 | Periplasmic1 Publication1 PublicationAdd BLAST | 104 |
GO - Cellular componenti
- cell division site Source: EcoCyc
- integral component of membrane Source: EcoliWiki
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoliWiki
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutants lacking the tol-pal cluster suffer delayed outer membrane invagination and contain large outer membrane blebs at constriction sites and cell poles (PubMed:17233825). Tol-pal mutants fail to complete division and form cell chains, and fail to process denuded peptidoglycans at the septum (PubMed:32152098).2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 23 | D → A: Decreases TolA-Pal interaction. 1 Publication | 1 | |
Mutagenesisi | 23 | D → E: No change in TolA-Pal interaction. 1 Publication | 1 | |
Mutagenesisi | 23 | D → R: Abolishes TolA-Pal interaction. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000129140 | 1 – 142 | Tol-Pal system protein TolRAdd BLAST | 142 |
Proteomic databases
jPOSTi | P0ABV6 |
PaxDbi | P0ABV6 |
PRIDEi | P0ABV6 |
Interactioni
Subunit structurei
The Tol-Pal system is composed of five core proteins: the inner membrane proteins TolA, TolQ and TolR, the periplasmic protein TolB and the outer membrane protein Pal. They form a network linking the inner and outer membranes and the peptidoglycan layer (PubMed:17233825). TolR forms homodimers (PubMed:10419942, PubMed:26354441).
Interacts with the N-terminal domain of TolA and with TolQ (PubMed:7744737, PubMed:10419942).
1 Publication3 PublicationsProtein-protein interaction databases
BioGRIDi | 4261829, 235 interactors 849704, 1 interactor |
ComplexPortali | CPX-5782, Tol-Pal cell envelope complex |
DIPi | DIP-48143N |
IntActi | P0ABV6, 4 interactors |
STRINGi | 511145.b0738 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0ABV6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domaini
The N-terminal transmembrane domain is involved in interaction with TolA and TolQ, while the central and C-terminal domains are involved in dimerization. The C-terminal domain is also involved in interaction with TolA and in association with the membranes (PubMed:10419942). May undergo large scale structural remodeling, where the N- and C-terminal sequences unfold in order for the protein to both reach and bind the peptidoglycan layer around 90 Angstroms away from the inner membrane (PubMed:26354441).2 Publications
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0848, Bacteria |
HOGENOMi | CLU_085305_1_3_6 |
InParanoidi | P0ABV6 |
PhylomeDBi | P0ABV6 |
Family and domain databases
HAMAPi | MF_02203, TolR, 1 hit |
InterProi | View protein in InterPro IPR003400, ExbD IPR014168, Tol-Pal_TolR |
PANTHERi | PTHR30558, PTHR30558, 1 hit |
Pfami | View protein in Pfam PF02472, ExbD, 1 hit |
TIGRFAMsi | TIGR02801, tolR, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ABV6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARARGRGRR DLKSEINIVP LLDVLLVLLL IFMATAPIIT QSVEVDLPDA
60 70 80 90 100
TESQAVSSND NPPVIVEVSG IGQYTVVVEK DRLERLPPEQ VVAEVSSRFK
110 120 130 140
ANPKTVFLIG GAKDVPYDEI IKALNLLHSA GVKSVGLMTQ PI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16489 Genomic DNA Translation: AAA83921.1 U00096 Genomic DNA Translation: AAC73832.1 AP009048 Genomic DNA Translation: BAA35404.1 |
PIRi | C25980, BVECTR |
RefSeqi | NP_415266.1, NC_000913.3 WP_000090097.1, NZ_STEB01000035.1 |
Genome annotation databases
EnsemblBacteriai | AAC73832; AAC73832; b0738 BAA35404; BAA35404; BAA35404 |
GeneIDi | 49585513 945328 |
KEGGi | ecj:JW0728 eco:b0738 |
PATRICi | fig|1411691.4.peg.1534 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16489 Genomic DNA Translation: AAA83921.1 U00096 Genomic DNA Translation: AAC73832.1 AP009048 Genomic DNA Translation: BAA35404.1 |
PIRi | C25980, BVECTR |
RefSeqi | NP_415266.1, NC_000913.3 WP_000090097.1, NZ_STEB01000035.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5BY4 | X-ray | 1.70 | A | 36-142 | [»] | |
SMRi | P0ABV6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261829, 235 interactors 849704, 1 interactor |
ComplexPortali | CPX-5782, Tol-Pal cell envelope complex |
DIPi | DIP-48143N |
IntActi | P0ABV6, 4 interactors |
STRINGi | 511145.b0738 |
Proteomic databases
jPOSTi | P0ABV6 |
PaxDbi | P0ABV6 |
PRIDEi | P0ABV6 |
Genome annotation databases
EnsemblBacteriai | AAC73832; AAC73832; b0738 BAA35404; BAA35404; BAA35404 |
GeneIDi | 49585513 945328 |
KEGGi | ecj:JW0728 eco:b0738 |
PATRICi | fig|1411691.4.peg.1534 |
Organism-specific databases
EchoBASEi | EB1004 |
Phylogenomic databases
eggNOGi | COG0848, Bacteria |
HOGENOMi | CLU_085305_1_3_6 |
InParanoidi | P0ABV6 |
PhylomeDBi | P0ABV6 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11011-MONOMER |
Miscellaneous databases
PROi | PR:P0ABV6 |
Family and domain databases
HAMAPi | MF_02203, TolR, 1 hit |
InterProi | View protein in InterPro IPR003400, ExbD IPR014168, Tol-Pal_TolR |
PANTHERi | PTHR30558, PTHR30558, 1 hit |
Pfami | View protein in Pfam PF02472, ExbD, 1 hit |
TIGRFAMsi | TIGR02801, tolR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TOLR_ECOLI | |
Accessioni | P0ABV6Primary (citable) accession number: P0ABV6 Secondary accession number(s): P05829 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | October 25, 2005 | |
Last modified: | December 2, 2020 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families