UniProtKB - P0ABU9 (TOLQ_ECOLI)
Protein
Tol-Pal system protein TolQ
Gene
tolQ
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity (PubMed:1683466, PubMed:17233825). Required, with TolR, for the proton motive force-dependent activation of TolA and for TolA-Pal interaction (PubMed:11722743). The Tol-Pal system is also required for polar localization of chemoreceptors clusters (PubMed:24720726). The system also appears to be required for the activity of several outer membrane-localized enzymes with cell wall remodeling activity (PubMed:32152098). Is involved in the uptake of group A colicins (colicins A, E1, E2, E3, and K) and in the uptake of filamentous phage DNA (PubMed:1683466, PubMed:3294803).1 Publication6 Publications
GO - Biological processi
- bacteriocin transport Source: EcoCyc
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-UniRule
- protein import Source: EcoliWiki
Keywordsi
Biological process | Cell cycle, Cell division |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11010-MONOMER |
Protein family/group databases
TCDBi | 1.A.30.2.2, the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Tol-Pal system protein TolQUniRule annotationCurated |
Gene namesi | Name:tolQ1 PublicationUniRule annotation Synonyms:fii1 Publication Ordered Locus Names:b0737, JW0727 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane UniRule annotation2 Publications; Multi-pass membrane protein UniRule annotation1 Publication1 Publication Note: Accumulates at cell constriction sites. Recruitment to the division site is dependent on FtsN activity.1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 15 | Periplasmic1 Publication1 PublicationAdd BLAST | 15 | |
Transmembranei | 16 – 36 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 37 – 138 | Cytoplasmic1 Publication1 PublicationAdd BLAST | 102 | |
Transmembranei | 139 – 159 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 160 – 170 | Periplasmic1 Publication1 PublicationAdd BLAST | 11 | |
Transmembranei | 171 – 191 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 192 – 230 | Cytoplasmic1 Publication2 PublicationsAdd BLAST | 39 |
GO - Cellular componenti
- cell division site Source: EcoCyc
- cell envelope Source: EcoCyc
- integral component of membrane Source: EcoliWiki
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutants lacking the tol-pal cluster suffer delayed outer membrane invagination and contain large outer membrane blebs at constriction sites and cell poles (PubMed:17233825). Tol-pal mutants fail to complete division and form cell chains, and fail to process denuded peptidoglycans at the septum (PubMed:32152098).2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 177 | A → V: Significantly reduces TolA-Pal interaction. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000145822 | 1 – 230 | Tol-Pal system protein TolQAdd BLAST | 230 |
Proteomic databases
jPOSTi | P0ABU9 |
PaxDbi | P0ABU9 |
PRIDEi | P0ABU9 |
Interactioni
Subunit structurei
The Tol-Pal system is composed of five core proteins: the inner membrane proteins TolA, TolQ and TolR, the periplasmic protein TolB and the outer membrane protein Pal. They form a network linking the inner and outer membranes and the peptidoglycan layer (PubMed:17233825). TolQ interacts with the N-terminal domain of TolA and with TolR (PubMed:7744737, PubMed:10419942).
1 Publication2 PublicationsProtein-protein interaction databases
BioGRIDi | 4259453, 275 interactors |
ComplexPortali | CPX-5782, Tol-Pal cell envelope complex |
DIPi | DIP-11008N |
STRINGi | 511145.b0737 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0811, Bacteria |
HOGENOMi | CLU_053325_2_2_6 |
InParanoidi | P0ABU9 |
PhylomeDBi | P0ABU9 |
Family and domain databases
HAMAPi | MF_02202, TolQ, 1 hit |
InterProi | View protein in InterPro IPR002898, MotA_ExbB_proton_chnl IPR014163, Tol-Pal_TolQ |
Pfami | View protein in Pfam PF01618, MotA_ExbB, 1 hit |
TIGRFAMsi | TIGR02796, tolQ, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ABU9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDMNILDLF LKASLLVKLI MLILIGFSIA SWAIIIQRTR ILNAAAREAE
60 70 80 90 100
AFEDKFWSGI ELSRLYQESQ GKRDNLTGSE QIFYSGFKEF VRLHRANSHA
110 120 130 140 150
PEAVVEGASR AMRISMNREL ENLETHIPFL GTVGSISPYI GLFGTVWGIM
160 170 180 190 200
HAFIALGAVK QATLQMVAPG IAEALIATAI GLFAAIPAVM AYNRLNQRVN
210 220 230
KLELNYDNFM EEFTAILHRQ AFTVSESNKG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16489 Genomic DNA Translation: AAA83919.1 U00096 Genomic DNA Translation: AAC73831.1 AP009048 Genomic DNA Translation: BAA35403.1 |
PIRi | B25980, BVECTQ |
RefSeqi | NP_415265.1, NC_000913.3 WP_000131314.1, NZ_STEB01000035.1 |
Genome annotation databases
EnsemblBacteriai | AAC73831; AAC73831; b0737 BAA35403; BAA35403; BAA35403 |
GeneIDi | 52076047 948900 |
KEGGi | ecj:JW0727 eco:b0737 |
PATRICi | fig|1411691.4.peg.1535 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16489 Genomic DNA Translation: AAA83919.1 U00096 Genomic DNA Translation: AAC73831.1 AP009048 Genomic DNA Translation: BAA35403.1 |
PIRi | B25980, BVECTQ |
RefSeqi | NP_415265.1, NC_000913.3 WP_000131314.1, NZ_STEB01000035.1 |
3D structure databases
SMRi | P0ABU9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259453, 275 interactors |
ComplexPortali | CPX-5782, Tol-Pal cell envelope complex |
DIPi | DIP-11008N |
STRINGi | 511145.b0737 |
Protein family/group databases
TCDBi | 1.A.30.2.2, the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily |
Proteomic databases
jPOSTi | P0ABU9 |
PaxDbi | P0ABU9 |
PRIDEi | P0ABU9 |
Genome annotation databases
EnsemblBacteriai | AAC73831; AAC73831; b0737 BAA35403; BAA35403; BAA35403 |
GeneIDi | 52076047 948900 |
KEGGi | ecj:JW0727 eco:b0737 |
PATRICi | fig|1411691.4.peg.1535 |
Organism-specific databases
EchoBASEi | EB1003 |
Phylogenomic databases
eggNOGi | COG0811, Bacteria |
HOGENOMi | CLU_053325_2_2_6 |
InParanoidi | P0ABU9 |
PhylomeDBi | P0ABU9 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11010-MONOMER |
Miscellaneous databases
PROi | PR:P0ABU9 |
Family and domain databases
HAMAPi | MF_02202, TolQ, 1 hit |
InterProi | View protein in InterPro IPR002898, MotA_ExbB_proton_chnl IPR014163, Tol-Pal_TolQ |
Pfami | View protein in Pfam PF01618, MotA_ExbB, 1 hit |
TIGRFAMsi | TIGR02796, tolQ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TOLQ_ECOLI | |
Accessioni | P0ABU9Primary (citable) accession number: P0ABU9 Secondary accession number(s): P05828 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | October 25, 2005 | |
Last modified: | December 2, 2020 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families