UniProtKB - P0ABU5 (ELBB_ECOLI)
Protein
Glyoxalase ElbB
Gene
elbB
Organism
Escherichia coli (strain K12)
Status
Functioni
Displays glyoxalase activity, catalyzing the conversion of glyoxal to glycolate (PubMed:26678554). However, this apparent glyoxalase activity may reflect a protein deglycase activity, which could be the primary function of this protein like other DJ-1 superfamily members such as PARK7, YajL, YhbO and HchA (Probable). Is not able to use methylglyoxal as substrate (PubMed:26678554).Curated1 Publication
Caution
Was originally thought (PubMed:9603997) to be involved in the early steps of isoprenoid biosynthesis.Curated
Catalytic activityi
Kineticsi
kcat is 0.48 min(-1) with glyoxal as substrate (at pH 7.4 and 37 degrees Celsius).1 Publication
- KM=1.21 mM for glyoxal (at pH 7.4 and 37 degrees Celsius)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 135 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- lyase activity Source: UniProtKB-KW
GO - Biological processi
- isoprenoid biosynthetic process Source: EcoliWiki
- positive regulation of isoprenoid metabolic process Source: EcoliWiki
Keywordsi
Molecular function | Lyase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11383-MONOMER MetaCyc:EG11383-MONOMER |
Protein family/group databases
MEROPSi | C56.975 |
Names & Taxonomyi
Protein namesi | Recommended name: Glyoxalase ElbB1 Publication (EC:4.2.1.-1 Publication)Alternative name(s): Sigma cross-reacting protein 27A1 Publication Short name: SCRP-27A1 Publication |
Gene namesi | Name:elbB1 Publication Synonyms:elb21 Publication, yzzB Ordered Locus Names:b3209, JW3176 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201681 | 1 – 217 | Glyoxalase ElbBAdd BLAST | 217 |
Proteomic databases
jPOSTi | P0ABU5 |
PaxDbi | P0ABU5 |
PRIDEi | P0ABU5 |
2D gel databases
SWISS-2DPAGEi | P0ABU5 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4262428, 13 interactors 852212, 5 interactors |
DIPi | DIP-48002N |
IntActi | P0ABU5, 6 interactors |
STRINGi | 511145.b3209 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0ABU5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ABU5 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase C56 family.Curated
Phylogenomic databases
eggNOGi | COG3155, Bacteria |
HOGENOMi | CLU_072952_1_0_6 |
InParanoidi | P0ABU5 |
PhylomeDBi | P0ABU5 |
Family and domain databases
Gene3Di | 3.40.50.880, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR026041, ElbB |
PANTHERi | PTHR10224:SF12, PTHR10224:SF12, 1 hit |
PIRSFi | PIRSF006320, Elb2, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ABU5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKIGVILSG CGVYDGSEIH EAVLTLLAIS RSGAQAVCFA PDKQQVDVIN
60 70 80 90 100
HLTGEAMTET RNVLIEAARI TRGEIRPLAQ ADAAELDALI VPGGFGAAKN
110 120 130 140 150
LSNFASLGSE CTVDRELKAL AQAMHQAGKP LGFMCIAPAM LPKIFDFPLR
160 170 180 190 200
LTIGTDIDTA EVLEEMGAEH VPCPVDDIVV DEDNKIVTTP AYMLAQNIAE
210
AASGIDKLVS RVLVLAE
Sequence cautioni
The sequence AAA58011 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 111 | C → S in BAA02487 (Ref. 1) Curated | 1 | |
Sequence conflicti | 195 – 217 | AQNIA…LVLAE → RRTLQKRRAALISWFPACWF WLNE in BAA02487 (Ref. 1) CuratedAdd BLAST | 23 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13188 Genomic DNA Translation: BAA02487.1 U18997 Genomic DNA Translation: AAA58011.1 Different initiation. U00096 Genomic DNA Translation: AAC76241.2 AP009048 Genomic DNA Translation: BAE77253.1 |
PIRi | C65112 |
RefSeqi | NP_417676.2, NC_000913.3 WP_001300411.1, NZ_SSZK01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC76241; AAC76241; b3209 BAE77253; BAE77253; BAE77253 |
GeneIDi | 48108747 947903 |
KEGGi | ecj:JW3176 eco:b3209 |
PATRICi | fig|1411691.4.peg.3522 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13188 Genomic DNA Translation: BAA02487.1 U18997 Genomic DNA Translation: AAA58011.1 Different initiation. U00096 Genomic DNA Translation: AAC76241.2 AP009048 Genomic DNA Translation: BAE77253.1 |
PIRi | C65112 |
RefSeqi | NP_417676.2, NC_000913.3 WP_001300411.1, NZ_SSZK01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OY1 | X-ray | 2.95 | A/B/C/D | 1-217 | [»] | |
1VHQ | X-ray | 1.65 | A/B | 1-217 | [»] | |
SMRi | P0ABU5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262428, 13 interactors 852212, 5 interactors |
DIPi | DIP-48002N |
IntActi | P0ABU5, 6 interactors |
STRINGi | 511145.b3209 |
Protein family/group databases
MEROPSi | C56.975 |
2D gel databases
SWISS-2DPAGEi | P0ABU5 |
Proteomic databases
jPOSTi | P0ABU5 |
PaxDbi | P0ABU5 |
PRIDEi | P0ABU5 |
Genome annotation databases
EnsemblBacteriai | AAC76241; AAC76241; b3209 BAE77253; BAE77253; BAE77253 |
GeneIDi | 48108747 947903 |
KEGGi | ecj:JW3176 eco:b3209 |
PATRICi | fig|1411691.4.peg.3522 |
Organism-specific databases
EchoBASEi | EB1356 |
Phylogenomic databases
eggNOGi | COG3155, Bacteria |
HOGENOMi | CLU_072952_1_0_6 |
InParanoidi | P0ABU5 |
PhylomeDBi | P0ABU5 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11383-MONOMER MetaCyc:EG11383-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0ABU5 |
PROi | PR:P0ABU5 |
Family and domain databases
Gene3Di | 3.40.50.880, 1 hit |
InterProi | View protein in InterPro IPR029062, Class_I_gatase-like IPR026041, ElbB |
PANTHERi | PTHR10224:SF12, PTHR10224:SF12, 1 hit |
PIRSFi | PIRSF006320, Elb2, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ELBB_ECOLI | |
Accessioni | P0ABU5Primary (citable) accession number: P0ABU5 Secondary accession number(s): P26428, P76673, Q2M903 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | October 25, 2005 | |
Last modified: | October 7, 2020 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries