UniProtKB - P0ABU2 (YCHF_ECOLI)
Protein
Ribosome-binding ATPase YchF
Gene
ychF
Organism
Escherichia coli (strain K12)
Status
Functioni
ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner. Does not hydrolyze GTP.UniRule annotation1 Publication
Cofactori
Mg2+By similarity
Activity regulationi
ATPase activity is stimulated by potassium and other monovalent cations. Stimulation is maximal in the presence of K+, followed by NH4+, Rb+, Na+ and Cs+.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 16 | MagnesiumBy similarity | 1 | |
Metal bindingi | 36 | MagnesiumBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 12 – 17 | ATPUniRule annotation | 6 |
GO - Molecular functioni
- ATPase activity Source: EcoCyc
- ATP binding Source: UniProtKB-UniRule
- enzyme inhibitor activity Source: EcoCyc
- GTP binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- ribosomal large subunit binding Source: EcoCyc
- ribosome binding Source: EcoCyc
GO - Biological processi
- response to oxidative stress Source: EcoCyc
Keywordsi
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11404-MONOMER MetaCyc:EG11404-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosome-binding ATPase YchFUniRule annotation |
Gene namesi | Name:ychFUniRule annotation Synonyms:engD, gtp1 Ordered Locus Names:b1203, JW1194 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 12 | N → A: Shows similar hydrolysis activities in the presence of K(+) or Na(+). 1 Publication | 1 | |
Mutagenesisi | 78 | K → A: Shows similar hydrolysis activities in the presence of K(+) or Na(+). 1 Publication | 1 | |
Mutagenesisi | 78 | K → R: Retains K(+)-dependent stimulation of ATPase activity and differentiates between K(+) and Na(+). 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000201675 | 2 – 363 | Ribosome-binding ATPase YchFAdd BLAST | 362 |
Proteomic databases
jPOSTi | P0ABU2 |
PaxDbi | P0ABU2 |
PRIDEi | P0ABU2 |
2D gel databases
SWISS-2DPAGEi | P0ABU2 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260106, 36 interactors |
IntActi | P0ABU2, 6 interactors |
STRINGi | 511145.b1203 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 256 | OBG-type GAdd BLAST | 254 | |
Domaini | 278 – 361 | TGSPROSITE-ProRule annotationAdd BLAST | 84 |
Sequence similaritiesi
Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | COG0012, Bacteria |
HOGENOMi | CLU_018395_0_1_6 |
InParanoidi | P0ABU2 |
PhylomeDBi | P0ABU2 |
Family and domain databases
CDDi | cd04867, TGS_YchF_OLA1, 1 hit cd01900, YchF, 1 hit |
Gene3Di | 1.10.150.300, 1 hit 3.10.20.30, 1 hit |
HAMAPi | MF_00944, YchF_OLA1_ATPase, 1 hit |
InterProi | View protein in InterPro IPR004396, ATPase_YchF/OLA1 IPR012675, Beta-grasp_dom_sf IPR031167, G_OBG IPR006073, GTP_binding_domain IPR027417, P-loop_NTPase IPR004095, TGS IPR012676, TGS-like IPR023192, TGS-like_dom_sf IPR013029, YchF_C IPR041706, YchF_N |
Pfami | View protein in Pfam PF01926, MMR_HSR1, 1 hit PF06071, YchF-GTPase_C, 1 hit |
PIRSFi | PIRSF006641, CHP00092, 1 hit |
PRINTSi | PR00326, GTP1OBG |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF81271, SSF81271, 1 hit |
TIGRFAMsi | TIGR00092, TIGR00092, 1 hit |
PROSITEi | View protein in PROSITE PS51710, G_OBG, 1 hit PS51880, TGS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0ABU2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGFKCGIVGL PNVGKSTLFN ALTKAGIEAA NFPFCTIEPN TGVVPMPDPR
60 70 80 90 100
LDQLAEIVKP QRTLPTTMEF VDIAGLVKGA SKGEGLGNQF LTNIRETEAI
110 120 130 140 150
GHVVRCFEND NIIHVSGKVN PADDIEVINT ELALADLDTC ERAIHRVQKK
160 170 180 190 200
AKGGDKDAKA ELAVLEKCLP QLENAGMLRA LDLSAEEKAA IRYLSFLTLK
210 220 230 240 250
PTMYIANVNE DGFENNPYLD QVREIAAKEG SVVVPVCAAV EADIAELDDE
260 270 280 290 300
ERDEFMQELG LEEPGLNRVI RAGYKLLNLQ TYFTAGVKEV RAWTIPVGAT
310 320 330 340 350
APQAAGKIHT DFEKGFIRAQ TISFEDFITY KGEQGAKEAG KMRAEGKDYI
360
VKDGDVMNFL FNV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74287.1 AP009048 Genomic DNA Translation: BAA36061.1 X61941 Genomic DNA Translation: CAA43946.1 U02423 Genomic DNA Translation: AAA67557.1 |
PIRi | H64866 |
RefSeqi | NP_415721.1, NC_000913.3 WP_000505866.1, NZ_STEB01000023.1 |
Genome annotation databases
EnsemblBacteriai | AAC74287; AAC74287; b1203 BAA36061; BAA36061; BAA36061 |
GeneIDi | 48725884 945769 |
KEGGi | ecj:JW1194 eco:b1203 |
PATRICi | fig|1411691.4.peg.1081 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74287.1 AP009048 Genomic DNA Translation: BAA36061.1 X61941 Genomic DNA Translation: CAA43946.1 U02423 Genomic DNA Translation: AAA67557.1 |
PIRi | H64866 |
RefSeqi | NP_415721.1, NC_000913.3 WP_000505866.1, NZ_STEB01000023.1 |
3D structure databases
SMRi | P0ABU2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260106, 36 interactors |
IntActi | P0ABU2, 6 interactors |
STRINGi | 511145.b1203 |
2D gel databases
SWISS-2DPAGEi | P0ABU2 |
Proteomic databases
jPOSTi | P0ABU2 |
PaxDbi | P0ABU2 |
PRIDEi | P0ABU2 |
Genome annotation databases
EnsemblBacteriai | AAC74287; AAC74287; b1203 BAA36061; BAA36061; BAA36061 |
GeneIDi | 48725884 945769 |
KEGGi | ecj:JW1194 eco:b1203 |
PATRICi | fig|1411691.4.peg.1081 |
Organism-specific databases
EchoBASEi | EB1376 |
Phylogenomic databases
eggNOGi | COG0012, Bacteria |
HOGENOMi | CLU_018395_0_1_6 |
InParanoidi | P0ABU2 |
PhylomeDBi | P0ABU2 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11404-MONOMER MetaCyc:EG11404-MONOMER |
Miscellaneous databases
PROi | PR:P0ABU2 |
Family and domain databases
CDDi | cd04867, TGS_YchF_OLA1, 1 hit cd01900, YchF, 1 hit |
Gene3Di | 1.10.150.300, 1 hit 3.10.20.30, 1 hit |
HAMAPi | MF_00944, YchF_OLA1_ATPase, 1 hit |
InterProi | View protein in InterPro IPR004396, ATPase_YchF/OLA1 IPR012675, Beta-grasp_dom_sf IPR031167, G_OBG IPR006073, GTP_binding_domain IPR027417, P-loop_NTPase IPR004095, TGS IPR012676, TGS-like IPR023192, TGS-like_dom_sf IPR013029, YchF_C IPR041706, YchF_N |
Pfami | View protein in Pfam PF01926, MMR_HSR1, 1 hit PF06071, YchF-GTPase_C, 1 hit |
PIRSFi | PIRSF006641, CHP00092, 1 hit |
PRINTSi | PR00326, GTP1OBG |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF81271, SSF81271, 1 hit |
TIGRFAMsi | TIGR00092, TIGR00092, 1 hit |
PROSITEi | View protein in PROSITE PS51710, G_OBG, 1 hit PS51880, TGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YCHF_ECOLI | |
Accessioni | P0ABU2Primary (citable) accession number: P0ABU2 Secondary accession number(s): P31216, P76018 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 126 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families