UniProtKB - P0ABS5 (DNAG_ECOLI)
Protein
DNA primase
Gene
dnaG
Organism
Escherichia coli (strain K12)
Status
Functioni
RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.UniRule annotation2 Publications
Catalytic activityi
- SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate.UniRule annotation2 Publications EC:2.7.7.101
Cofactori
Protein has several cofactor binding sites:- Zn2+UniRule annotation2 PublicationsNote: Binds 1 zinc ion per monomer.UniRule annotation2 Publications
- Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation
Activity regulationi
Activity can be regulated by an intermolecular trans association between the zinc-binding domain and the catalytic core domain of two different primase molecules.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 265 | Magnesium 1; catalyticUniRule annotation | 1 | |
Metal bindingi | 309 | Magnesium 1; catalyticUniRule annotation | 1 | |
Metal bindingi | 309 | Magnesium 2UniRule annotation | 1 | |
Metal bindingi | 311 | Magnesium 2UniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 40 – 64 | CHC2-typeUniRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA primase activity Source: EcoliWiki
- zinc ion binding Source: EcoliWiki
GO - Biological processi
- DNA replication, synthesis of RNA primer Source: EcoliWiki
Keywordsi
Molecular function | DNA-binding, Nucleotidyltransferase, Transferase |
Biological process | DNA replication, Transcription |
Ligand | Magnesium, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10239-MONOMER MetaCyc:EG10239-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dnaGUniRule annotation Synonyms:dnaP, parB Ordered Locus Names:b3066, JW3038 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- cytoplasm Source: EcoliWiki
- primosome complex Source: UniProtKB-KW
Keywords - Cellular componenti
DNA-directed RNA polymerase, PrimosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 165 | W → A: Abolishes DNA-binding and primer synthesis. 1 Publication | 1 | |
Mutagenesisi | 199 | R → A: Abolishes primer synthesis but can still bind DNA. Abolishes DNA-binding; when associated with A-201. 1 Publication | 1 | |
Mutagenesisi | 201 | R → A: Abolishes primer synthesis but can still bind DNA. Abolishes DNA-binding; when associated with A-199. 1 Publication | 1 | |
Mutagenesisi | 576 | Q → A: Decreases interaction with DnaB and primase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000180490 | 1 – 581 | DNA primaseAdd BLAST | 581 |
Proteomic databases
jPOSTi | P0ABS5 |
PaxDbi | P0ABS5 |
PRIDEi | P0ABS5 |
Interactioni
Subunit structurei
Monomer.
Interacts with DnaB. The primase-helicase complex is composed of up to three DnaG bound to one DnaB hexamer.
UniRule annotation6 PublicationsBinary interactionsi
Hide detailsP0ABS5
With | #Exp. | IntAct |
---|---|---|
dnaB [P0ACB0] | 3 | EBI-549259,EBI-548978 |
rplA [P0A7L0] | 2 | EBI-549259,EBI-543771 |
ssb [P0AGE0] | 2 | EBI-549259,EBI-1118620 |
Protein-protein interaction databases
BioGRIDi | 4262146, 230 interactors 851886, 1 interactor |
ComplexPortali | CPX-1933, DnaB-DnaG primase-helicase complex CPX-5909, Replication restart primosome complex, priAC variant CPX-5910, Replication restart primosome complex, priAB variant CPX-5911, Replication restart primosome complex, priC-rep variant |
DIPi | DIP-47954N |
IntActi | P0ABS5, 17 interactors |
STRINGi | 511145.b3066 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0ABS5 |
SMRi | P0ABS5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ABS5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 259 – 341 | ToprimUniRule annotationAdd BLAST | 83 |
Domaini
Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.UniRule annotation3 Publications
Sequence similaritiesi
Belongs to the DnaG primase family.UniRule annotation
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 40 – 64 | CHC2-typeUniRule annotationAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | COG0358, Bacteria |
HOGENOMi | CLU_013501_5_4_6 |
InParanoidi | P0ABS5 |
PhylomeDBi | P0ABS5 |
Family and domain databases
CDDi | cd03364, TOPRIM_DnaG_primases, 1 hit |
DisProti | DP02134 |
Gene3Di | 1.10.860.10, 1 hit 3.90.580.10, 1 hit 3.90.980.10, 1 hit |
HAMAPi | MF_00974, DNA_primase_DnaG, 1 hit |
InterProi | View protein in InterPro IPR016136, DNA_helicase_N/primase_C IPR013264, DNA_primase_core_N IPR037068, DNA_primase_core_N_sf IPR019475, DNA_primase_DnaB-bd IPR006295, DNA_primase_DnaG IPR013173, DNA_primase_DnaG_DnaB-bd_dom IPR036977, DNA_primase_Znf_CHC2 IPR030846, DnaG_bac IPR034151, TOPRIM_DnaG_bac IPR006171, TOPRIM_domain IPR002694, Znf_CHC2 |
Pfami | View protein in Pfam PF10410, DnaB_bind, 1 hit PF08278, DnaG_DnaB_bind, 1 hit PF08275, Toprim_N, 1 hit PF01807, zf-CHC2, 1 hit |
PIRSFi | PIRSF002811, DnaG, 1 hit |
SMARTi | View protein in SMART SM00766, DnaG_DnaB_bind, 1 hit SM00493, TOPRIM, 1 hit SM00400, ZnF_CHCC, 1 hit |
TIGRFAMsi | TIGR01391, dnaG, 1 hit |
PROSITEi | View protein in PROSITE PS50880, TOPRIM, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ABS5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGRIPRVFI NDLLARTDIV DLIDARVKLK KQGKNFHACC PFHNEKTPSF
60 70 80 90 100
TVNGEKQFYH CFGCGAHGNA IDFLMNYDKL EFVETVEELA AMHNLEVPFE
110 120 130 140 150
AGSGPSQIER HQRQTLYQLM DGLNTFYQQS LQQPVATSAR QYLEKRGLSH
160 170 180 190 200
EVIARFAIGF APPGWDNVLK RFGGNPENRQ SLIDAGMLVT NDQGRSYDRF
210 220 230 240 250
RERVMFPIRD KRGRVIGFGG RVLGNDTPKY LNSPETDIFH KGRQLYGLYE
260 270 280 290 300
AQQDNAEPNR LLVVEGYMDV VALAQYGINY AVASLGTSTT ADHIQLLFRA
310 320 330 340 350
TNNVICCYDG DRAGRDAAWR ALETALPYMT DGRQLRFMFL PDGEDPDTLV
360 370 380 390 400
RKEGKEAFEA RMEQAMPLSA FLFNSLMPQV DLSTPDGRAR LSTLALPLIS
410 420 430 440 450
QVPGETLRIY LRQELGNKLG ILDDSQLERL MPKAAESGVS RPVPQLKRTT
460 470 480 490 500
MRILIGLLVQ NPELATLVPP LENLDENKLP GLGLFRELVN TCLSQPGLTT
510 520 530 540 550
GQLLEHYRGT NNAATLEKLS MWDDIADKNI AEQTFTDSLN HMFDSLLELR
560 570 580
QEELIARERT HGLSNEERLE LWTLNQELAK K
Sequence cautioni
The sequence CAA23531 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 24 | D → N in CAA23636 (PubMed:6222240).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01687 Genomic DNA Translation: AAA24600.1 V00274 Genomic DNA Translation: CAA23531.1 Frameshift. U28379 Genomic DNA Translation: AAA89146.1 U00096 Genomic DNA Translation: AAC76102.1 AP009048 Genomic DNA Translation: BAE77117.1 V00346 Genomic DNA Translation: CAA23636.1 |
PIRi | A03423, RYEC2 |
RefSeqi | NP_417538.1, NC_000913.3 WP_000918827.1, NZ_STEB01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC76102; AAC76102; b3066 BAE77117; BAE77117; BAE77117 |
GeneIDi | 58390557 947570 |
KEGGi | ecj:JW3038 eco:b3066 |
PATRICi | fig|1411691.4.peg.3664 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01687 Genomic DNA Translation: AAA24600.1 V00274 Genomic DNA Translation: CAA23531.1 Frameshift. U28379 Genomic DNA Translation: AAA89146.1 U00096 Genomic DNA Translation: AAC76102.1 AP009048 Genomic DNA Translation: BAE77117.1 V00346 Genomic DNA Translation: CAA23636.1 |
PIRi | A03423, RYEC2 |
RefSeqi | NP_417538.1, NC_000913.3 WP_000918827.1, NZ_STEB01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DD9 | X-ray | 1.60 | A | 111-433 | [»] | |
1DDE | X-ray | 1.70 | A | 111-433 | [»] | |
1EQN | X-ray | 2.90 | A/B/C/D/E | 111-429 | [»] | |
1T3W | X-ray | 2.80 | A/B | 434-581 | [»] | |
2HAJ | NMR | - | A | 434-581 | [»] | |
3B39 | X-ray | 2.35 | A/B | 111-429 | [»] | |
6CBR | X-ray | 1.50 | A/B | 434-581 | [»] | |
6CBS | X-ray | 1.85 | A/B | 434-581 | [»] | |
6CBT | X-ray | 2.10 | A/B | 434-581 | [»] | |
BMRBi | P0ABS5 | |||||
SMRi | P0ABS5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262146, 230 interactors 851886, 1 interactor |
ComplexPortali | CPX-1933, DnaB-DnaG primase-helicase complex CPX-5909, Replication restart primosome complex, priAC variant CPX-5910, Replication restart primosome complex, priAB variant CPX-5911, Replication restart primosome complex, priC-rep variant |
DIPi | DIP-47954N |
IntActi | P0ABS5, 17 interactors |
STRINGi | 511145.b3066 |
Proteomic databases
jPOSTi | P0ABS5 |
PaxDbi | P0ABS5 |
PRIDEi | P0ABS5 |
Genome annotation databases
EnsemblBacteriai | AAC76102; AAC76102; b3066 BAE77117; BAE77117; BAE77117 |
GeneIDi | 58390557 947570 |
KEGGi | ecj:JW3038 eco:b3066 |
PATRICi | fig|1411691.4.peg.3664 |
Organism-specific databases
EchoBASEi | EB0235 |
Phylogenomic databases
eggNOGi | COG0358, Bacteria |
HOGENOMi | CLU_013501_5_4_6 |
InParanoidi | P0ABS5 |
PhylomeDBi | P0ABS5 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10239-MONOMER MetaCyc:EG10239-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0ABS5 |
PROi | PR:P0ABS5 |
Family and domain databases
CDDi | cd03364, TOPRIM_DnaG_primases, 1 hit |
DisProti | DP02134 |
Gene3Di | 1.10.860.10, 1 hit 3.90.580.10, 1 hit 3.90.980.10, 1 hit |
HAMAPi | MF_00974, DNA_primase_DnaG, 1 hit |
InterProi | View protein in InterPro IPR016136, DNA_helicase_N/primase_C IPR013264, DNA_primase_core_N IPR037068, DNA_primase_core_N_sf IPR019475, DNA_primase_DnaB-bd IPR006295, DNA_primase_DnaG IPR013173, DNA_primase_DnaG_DnaB-bd_dom IPR036977, DNA_primase_Znf_CHC2 IPR030846, DnaG_bac IPR034151, TOPRIM_DnaG_bac IPR006171, TOPRIM_domain IPR002694, Znf_CHC2 |
Pfami | View protein in Pfam PF10410, DnaB_bind, 1 hit PF08278, DnaG_DnaB_bind, 1 hit PF08275, Toprim_N, 1 hit PF01807, zf-CHC2, 1 hit |
PIRSFi | PIRSF002811, DnaG, 1 hit |
SMARTi | View protein in SMART SM00766, DnaG_DnaB_bind, 1 hit SM00493, TOPRIM, 1 hit SM00400, ZnF_CHCC, 1 hit |
TIGRFAMsi | TIGR01391, dnaG, 1 hit |
PROSITEi | View protein in PROSITE PS50880, TOPRIM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DNAG_ECOLI | |
Accessioni | P0ABS5Primary (citable) accession number: P0ABS5 Secondary accession number(s): P02922 Q47613 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | April 7, 2021 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families