UniProtKB - P0ABR5 (HCAE_ECOLI)
Protein
3-phenylpropionate/cinnamic acid dioxygenase subunit alpha
Gene
hcaE
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively.1 Publication
Catalytic activityi
- EC:1.14.12.19
- (E)-cinnamate + H+ + NADH + O2 = (2E)-3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)prop-2-enoate + NAD+EC:1.14.12.19
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] clusterCuratedNote: Binds 1 [2Fe-2S] cluster.Curated
- Fe cationCuratedNote: Binds 1 Fe cation.Curated
: 3-phenylpropanoate degradation Pathwayi
This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 85 | Iron-sulfur (2Fe-2S)By similarity | 1 | |
Metal bindingi | 87 | Iron-sulfur (2Fe-2S); via pros nitrogenBy similarity | 1 | |
Metal bindingi | 105 | Iron-sulfur (2Fe-2S)By similarity | 1 | |
Metal bindingi | 108 | Iron-sulfur (2Fe-2S); via pros nitrogenBy similarity | 1 | |
Metal bindingi | 213 | IronBy similarity | 1 | |
Metal bindingi | 218 | IronBy similarity | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- 3-phenylpropionate dioxygenase activity Source: UniProtKB-UniRule
- iron ion binding Source: UniProtKB-UniRule
GO - Biological processi
- 3-phenylpropionate catabolic process Source: EcoCyc
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Aromatic hydrocarbons catabolism |
Ligand | 2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:PHENYLPRODIOXY-MONOMER MetaCyc:PHENYLPRODIOXY-MONOMER |
UniPathwayi | UPA00714 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:hcaE Synonyms:digA, hcaA, hcaA1, phdC1, yfhU Ordered Locus Names:b2538, JW2522 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085061 | 1 – 453 | 3-phenylpropionate/cinnamic acid dioxygenase subunit alphaAdd BLAST | 453 |
Proteomic databases
PaxDbi | P0ABR5 |
PRIDEi | P0ABR5 |
Interactioni
Subunit structurei
This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (HcaE and HcaF), a ferredoxin (HcaC) and a ferredoxin reductase (HcaD).
Protein-protein interaction databases
BioGRIDi | 4261577, 13 interactors |
ComplexPortali | CPX-5161, 3-phenylpropionate/cinnamic acid dioxygenase |
IntActi | P0ABR5, 5 interactors |
STRINGi | 511145.b2538 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 44 – 142 | RieskeAdd BLAST | 99 |
Sequence similaritiesi
Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family.Curated
Phylogenomic databases
eggNOGi | COG4638, Bacteria |
HOGENOMi | CLU_026244_4_0_6 |
InParanoidi | P0ABR5 |
PhylomeDBi | P0ABR5 |
Family and domain databases
CDDi | cd08881, RHO_alpha_C_NDO-like, 1 hit |
Gene3Di | 2.102.10.10, 1 hit |
HAMAPi | MF_01648, HcaE, 1 hit |
InterProi | View protein in InterPro IPR020875, HcaE IPR043266, RHO_NdoB-like_C IPR017941, Rieske_2Fe-2S IPR036922, Rieske_2Fe-2S_sf IPR015881, Ring-hydroxy_dOase_2Fe2S_BS IPR015879, Ring_hydroxy_dOase_asu_C_dom IPR001663, Rng_hydr_dOase-A |
Pfami | View protein in Pfam PF00355, Rieske, 1 hit PF00848, Ring_hydroxyl_A, 1 hit |
PRINTSi | PR00090, RNGDIOXGNASE |
SUPFAMi | SSF50022, SSF50022, 1 hit |
PROSITEi | View protein in PROSITE PS51296, RIESKE, 1 hit PS00570, RING_HYDROXYL_ALPHA, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ABR5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTPSDLNIY QLIDTQNGRV TPRIYTDPDI YQLELERIFG RCWLFLAHES
60 70 80 90 100
QIPKPGDFFN TYMGEDAVVV VRQKDGSIKA FLNQCRHRAM RVSYADCGNT
110 120 130 140 150
RAFTCPYHGW SYGINGELID VPLEPRAYPQ GLCKSHWGLN EVPCVESYKG
160 170 180 190 200
LIFGNWDTSA PGLRDYLGDI AWYLDGMLDR REGGTEIVGG VQKWVINCNW
210 220 230 240 250
KFPAEQFASD QYHALFSHAS AVQVLGAKDD GSDKRLGDGQ TARPVWETAK
260 270 280 290 300
DALQFGQDGH GSGFFFTEKP DANVWVDGAV SSYYRETYAE AEQRLGEVRA
310 320 330 340 350
LRLAGHNNIF PTLSWLNGTA TLRVWHPRGP DQVEVWAFCI TDKAASDEVK
360 370 380 390 400
AAFENSATRA FGPAGFLEQD DSENWCEIQK LLKGHRARNS KLCLEMGLGQ
410 420 430 440 450
EKRRDDGIPG ITNYIFSETA ARGMYQRWAD LLSSESWQEV LDKTAAYQQE
VMK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 20 | V → A in CAA86018 (Ref. 1) Curated | 1 | |
Sequence conflicti | 384 – 453 | GHRAR…QEVMK → ATAPATANCVWKWGLVRKSA ATTAFLALLTISFQKLPLVE CTNAGPIF in CAA86018 (Ref. 1) CuratedAdd BLAST | 70 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z37966 Genomic DNA Translation: CAA86018.1 U00096 Genomic DNA Translation: AAC75591.1 AP009048 Genomic DNA Translation: BAA16441.1 |
PIRi | A65031 |
RefSeqi | NP_417033.1, NC_000913.3 WP_000211172.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75591; AAC75591; b2538 BAA16441; BAA16441; BAA16441 |
GeneIDi | 57732360 946998 |
KEGGi | ecj:JW2522 eco:b2538 |
PATRICi | fig|1411691.4.peg.4196 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z37966 Genomic DNA Translation: CAA86018.1 U00096 Genomic DNA Translation: AAC75591.1 AP009048 Genomic DNA Translation: BAA16441.1 |
PIRi | A65031 |
RefSeqi | NP_417033.1, NC_000913.3 WP_000211172.1, NZ_STEB01000011.1 |
3D structure databases
SMRi | P0ABR5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261577, 13 interactors |
ComplexPortali | CPX-5161, 3-phenylpropionate/cinnamic acid dioxygenase |
IntActi | P0ABR5, 5 interactors |
STRINGi | 511145.b2538 |
Proteomic databases
PaxDbi | P0ABR5 |
PRIDEi | P0ABR5 |
Genome annotation databases
EnsemblBacteriai | AAC75591; AAC75591; b2538 BAA16441; BAA16441; BAA16441 |
GeneIDi | 57732360 946998 |
KEGGi | ecj:JW2522 eco:b2538 |
PATRICi | fig|1411691.4.peg.4196 |
Organism-specific databases
EchoBASEi | EB3229 |
Phylogenomic databases
eggNOGi | COG4638, Bacteria |
HOGENOMi | CLU_026244_4_0_6 |
InParanoidi | P0ABR5 |
PhylomeDBi | P0ABR5 |
Enzyme and pathway databases
UniPathwayi | UPA00714 |
BioCyci | EcoCyc:PHENYLPRODIOXY-MONOMER MetaCyc:PHENYLPRODIOXY-MONOMER |
Miscellaneous databases
PROi | PR:P0ABR5 |
Family and domain databases
CDDi | cd08881, RHO_alpha_C_NDO-like, 1 hit |
Gene3Di | 2.102.10.10, 1 hit |
HAMAPi | MF_01648, HcaE, 1 hit |
InterProi | View protein in InterPro IPR020875, HcaE IPR043266, RHO_NdoB-like_C IPR017941, Rieske_2Fe-2S IPR036922, Rieske_2Fe-2S_sf IPR015881, Ring-hydroxy_dOase_2Fe2S_BS IPR015879, Ring_hydroxy_dOase_asu_C_dom IPR001663, Rng_hydr_dOase-A |
Pfami | View protein in Pfam PF00355, Rieske, 1 hit PF00848, Ring_hydroxyl_A, 1 hit |
PRINTSi | PR00090, RNGDIOXGNASE |
SUPFAMi | SSF50022, SSF50022, 1 hit |
PROSITEi | View protein in PROSITE PS51296, RIESKE, 1 hit PS00570, RING_HYDROXYL_ALPHA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HCAE_ECOLI | |
Accessioni | P0ABR5Primary (citable) accession number: P0ABR5 Secondary accession number(s): P77590, P78203, Q47139 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | February 10, 2021 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families