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Protein

2-hydroxy-3-oxopropionate reductase

Gene

garR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of tatronate semialdehyde to D-glycerate.UniRule annotation1 Publication

Catalytic activityi

D-glycerate + NAD(P)+ = 2-hydroxy-3-oxopropanoate + NAD(P)H.UniRule annotation1 Publication

Pathwayi: galactarate degradation

This protein is involved in step 3 of the subpathway that synthesizes D-glycerate from galactarate.UniRule annotation1 Publication
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Galactarate dehydratase (L-threo-forming) (garD)
  2. 5-keto-4-deoxy-D-glucarate aldolase (garL)
  3. 2-hydroxy-3-oxopropionate reductase (garR)
This subpathway is part of the pathway galactarate degradation, which is itself part of Carbohydrate acid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glycerate from galactarate, the pathway galactarate degradation and in Carbohydrate acid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95NADUniRule annotation1
Active sitei170UniRule annotation1
Binding sitei238NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi4 – 18NADUniRule annotationAdd BLAST15

GO - Molecular functioni

  • 2-hydroxy-3-oxopropionate reductase activity Source: EcoCyc
  • NAD binding Source: InterPro
  • NADP binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciEcoCyc:TSA-REDUCT-MONOMER
MetaCyc:TSA-REDUCT-MONOMER
UniPathwayi
UPA00565;UER00631

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxy-3-oxopropionate reductaseUniRule annotation (EC:1.1.1.60UniRule annotation1 Publication)
Alternative name(s):
Tartronate semialdehyde reductase1 PublicationUniRule annotation
Short name:
TSAR1 PublicationUniRule annotation
Gene namesi
Name:garR1 PublicationUniRule annotation
Synonyms:yhaE
Ordered Locus Names:b3125, JW5526
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11176 garR

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001730591 – 2942-hydroxy-3-oxopropionate reductaseAdd BLAST294

Proteomic databases

EPDiP0ABQ2
PaxDbiP0ABQ2
PRIDEiP0ABQ2

Expressioni

Inductioni

Induced by D-galactarate, D-glucarate and D-glycerate.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259488, 48 interactors
DIPiDIP-9742N
STRINGi316385.ECDH10B_3298

Structurei

3D structure databases

ProteinModelPortaliP0ABQ2
SMRiP0ABQ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HIBADH-related family. 2-hydroxy-3-oxopropionate reductase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CF3 Bacteria
COG2084 LUCA
HOGENOMiHOG000219608
InParanoidiP0ABQ2
KOiK00042
PhylomeDBiP0ABQ2

Family and domain databases

Gene3Di1.10.1040.10, 1 hit
HAMAPiMF_02032 Tartronate_sem_reduc, 1 hit
InterProiView protein in InterPro
IPR002204 3-OH-isobutyrate_DH-rel_CS
IPR008927 6-PGluconate_DH-like_C_sf
IPR013328 6PGD_dom2
IPR006115 6PGDH_NADP-bd
IPR015815 HIBADH-related
IPR036291 NAD(P)-bd_dom_sf
IPR029154 NADP-bd
IPR006398 Tartro_sem_red
PfamiView protein in Pfam
PF14833 NAD_binding_11, 1 hit
PF03446 NAD_binding_2, 1 hit
PIRSFiPIRSF000103 HIBADH, 1 hit
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01505 tartro_sem_red, 1 hit
PROSITEiView protein in PROSITE
PS00895 3_HYDROXYISOBUT_DH, 1 hit

Sequencei

Sequence statusi: Complete.

P0ABQ2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKVGFIGLGI MGKPMSKNLL KAGYSLVVAD RNPEAIADVI AAGAETASTA
60 70 80 90 100
KAIAEQCDVI ITMLPNSPHV KEVALGENGI IEGAKPGTVL IDMSSIAPLA
110 120 130 140 150
SREISEALKA KGIDMLDAPV SGGEPKAIDG TLSVMVGGDK AIFDKYYDLM
160 170 180 190 200
KAMAGSVVHT GEIGAGNVTK LANQVIVALN IAAMSEALTL ATKAGVNPDL
210 220 230 240 250
VYQAIRGGLA GSTVLDAKAP MVMDRNFKPG FRIDLHIKDL ANALDTSHGV
260 270 280 290
GAQLPLTAAV MEMMQALRAD GLGTADHSAL ACYYEKLAKV EVTR
Length:294
Mass (Da):30,427
Last modified:November 8, 2005 - v1
Checksum:i17DA392C2253278C
GO

Sequence cautioni

The sequence AAA57928 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAA14238 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE77172 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90212 Genomic DNA Translation: BAA14238.1 Different initiation.
U18997 Genomic DNA Translation: AAA57928.1 Different initiation.
U00096 Genomic DNA Translation: AAC76159.3
AP009048 Genomic DNA Translation: BAE77172.1 Different initiation.
RefSeqiNP_417594.3, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAC76159; AAC76159; b3125
BAE77172; BAE77172; BAE77172
GeneIDi947631
KEGGiecj:JW5526
eco:b3125
PATRICifig|511145.12.peg.3218

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90212 Genomic DNA Translation: BAA14238.1 Different initiation.
U18997 Genomic DNA Translation: AAA57928.1 Different initiation.
U00096 Genomic DNA Translation: AAC76159.3
AP009048 Genomic DNA Translation: BAE77172.1 Different initiation.
RefSeqiNP_417594.3, NC_000913.3

3D structure databases

ProteinModelPortaliP0ABQ2
SMRiP0ABQ2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259488, 48 interactors
DIPiDIP-9742N
STRINGi316385.ECDH10B_3298

Proteomic databases

EPDiP0ABQ2
PaxDbiP0ABQ2
PRIDEiP0ABQ2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76159; AAC76159; b3125
BAE77172; BAE77172; BAE77172
GeneIDi947631
KEGGiecj:JW5526
eco:b3125
PATRICifig|511145.12.peg.3218

Organism-specific databases

EchoBASEiEB1163
EcoGeneiEG11176 garR

Phylogenomic databases

eggNOGiENOG4105CF3 Bacteria
COG2084 LUCA
HOGENOMiHOG000219608
InParanoidiP0ABQ2
KOiK00042
PhylomeDBiP0ABQ2

Enzyme and pathway databases

UniPathwayi
UPA00565;UER00631

BioCyciEcoCyc:TSA-REDUCT-MONOMER
MetaCyc:TSA-REDUCT-MONOMER

Miscellaneous databases

PROiPR:P0ABQ2

Family and domain databases

Gene3Di1.10.1040.10, 1 hit
HAMAPiMF_02032 Tartronate_sem_reduc, 1 hit
InterProiView protein in InterPro
IPR002204 3-OH-isobutyrate_DH-rel_CS
IPR008927 6-PGluconate_DH-like_C_sf
IPR013328 6PGD_dom2
IPR006115 6PGDH_NADP-bd
IPR015815 HIBADH-related
IPR036291 NAD(P)-bd_dom_sf
IPR029154 NADP-bd
IPR006398 Tartro_sem_red
PfamiView protein in Pfam
PF14833 NAD_binding_11, 1 hit
PF03446 NAD_binding_2, 1 hit
PIRSFiPIRSF000103 HIBADH, 1 hit
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01505 tartro_sem_red, 1 hit
PROSITEiView protein in PROSITE
PS00895 3_HYDROXYISOBUT_DH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGARR_ECOLI
AccessioniPrimary (citable) accession number: P0ABQ2
Secondary accession number(s): P23523, Q2M984
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: November 7, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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