UniProtKB - P0ABN1 (KDGL_ECOLI)
Protein
Diacylglycerol kinase
Gene
dgkA
Organism
Escherichia coli (strain K12)
Status
Functioni
Recycling of diacylglycerol produced during the turnover of membrane phospholipid.
Catalytic activityi
- EC:2.7.1.107
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- diacylglycerol kinase activity Source: EcoCyc
- identical protein binding Source: EcoCyc
- metal ion binding Source: EcoCyc
GO - Biological processi
- phosphatidic acid biosynthetic process Source: EcoCyc
- response to UV Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:DIACYLGLYKIN-MONOMER MetaCyc:DIACYLGLYKIN-MONOMER |
BRENDAi | 2.7.1.107, 2026 |
Chemistry databases
SwissLipidsi | SLP:000001806 |
Names & Taxonomyi
Protein namesi | Recommended name: Diacylglycerol kinase (EC:2.7.1.107)Short name: DAGK Alternative name(s): Diglyceride kinase Short name: DGK |
Gene namesi | Name:dgkA Ordered Locus Names:b4042, JW4002 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 33 | CytoplasmicCuratedAdd BLAST | 32 | |
Transmembranei | 34 – 49 | HelicalCuratedAdd BLAST | 16 | |
Topological domaini | 50 – 52 | PeriplasmicCurated | 3 | |
Transmembranei | 53 – 69 | HelicalCuratedAdd BLAST | 17 | |
Topological domaini | 70 – 95 | CytoplasmicCuratedAdd BLAST | 26 | |
Transmembranei | 96 – 119 | HelicalCuratedAdd BLAST | 24 | |
Topological domaini | 120 – 122 | PeriplasmicCurated | 3 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000195260 | 2 – 122 | Diacylglycerol kinaseAdd BLAST | 121 |
Proteomic databases
jPOSTi | P0ABN1 |
PaxDbi | P0ABN1 |
PRIDEi | P0ABN1 |
Interactioni
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4261719, 281 interactors 852837, 2 interactors |
DIPi | DIP-60228N |
STRINGi | 511145.b4042 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0ABN1 |
SMRi | P0ABN1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ABN1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the bacterial diacylglycerol kinase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0818, Bacteria |
HOGENOMi | CLU_112343_3_1_6 |
InParanoidi | P0ABN1 |
PhylomeDBi | P0ABN1 |
Family and domain databases
CDDi | cd14264, DAGK_IM, 1 hit |
Gene3Di | 1.10.3830.10, 1 hit |
InterProi | View protein in InterPro IPR000829, DAGK IPR033718, DAGK_prok IPR036945, DAGK_sf |
PANTHERi | PTHR34299, PTHR34299, 1 hit |
Pfami | View protein in Pfam PF01219, DAGK_prokar, 1 hit |
PROSITEi | View protein in PROSITE PS01069, DAGK_PROKAR, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0ABN1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD
60 70 80 90 100
VDAITRVLLI SSVMLVMIVE ILNSAIEAVV DRIGSEYHEL SGRAKDMGSA
110 120
AVLIAIIVAV ITWCILLWSH FG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K00127 Genomic DNA Translation: AAA24394.1 U00006 Genomic DNA Translation: AAC43136.1 U00096 Genomic DNA Translation: AAC77012.1 AP009048 Genomic DNA Translation: BAE78044.1 |
PIRi | A00667, KIECDG |
RefSeqi | NP_418466.1, NC_000913.3 WP_000002907.1, NZ_STEB01000022.1 |
Genome annotation databases
EnsemblBacteriai | AAC77012; AAC77012; b4042 BAE78044; BAE78044; BAE78044 |
GeneIDi | 48723107 948543 |
KEGGi | ecj:JW4002 eco:b4042 |
PATRICi | fig|1411691.4.peg.2666 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K00127 Genomic DNA Translation: AAA24394.1 U00006 Genomic DNA Translation: AAC43136.1 U00096 Genomic DNA Translation: AAC77012.1 AP009048 Genomic DNA Translation: BAE78044.1 |
PIRi | A00667, KIECDG |
RefSeqi | NP_418466.1, NC_000913.3 WP_000002907.1, NZ_STEB01000022.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2KDC | NMR | - | A/B/C | 2-122 | [»] | |
3ZE3 | X-ray | 2.05 | A/B/C/D/E/F | 2-122 | [»] | |
3ZE4 | X-ray | 3.70 | A/B/C | 2-122 | [»] | |
3ZE5 | X-ray | 3.10 | A/B/C | 2-122 | [»] | |
4BPD | X-ray | 3.30 | A/B/C/D/E/F | 2-122 | [»] | |
4BRB | X-ray | 2.55 | A/B/C/D/E/F | 2-122 | [»] | |
4BRR | X-ray | 2.44 | A/B/C/D/E/F | 2-122 | [»] | |
4CJZ | X-ray | 3.25 | A/B/C | 2-122 | [»] | |
4CK0 | X-ray | 2.92 | A/B/C | 2-122 | [»] | |
4D2E | X-ray | 2.28 | A/B/C/D/E/F | 2-122 | [»] | |
4UP6 | X-ray | 3.80 | A/B/C | 2-122 | [»] | |
4UXW | X-ray | 3.15 | A/B/C | 2-122 | [»] | |
4UXX | X-ray | 2.70 | A/B/C | 2-122 | [»] | |
4UXZ | X-ray | 2.18 | A/B/C/D/E/F | 2-122 | [»] | |
4UYO | X-ray | 2.18 | A/B/C/D/E/F | 2-122 | [»] | |
5D56 | X-ray | 2.80 | A/B/C/D/E/F | 2-122 | [»] | |
5D57 | X-ray | 2.80 | A/B/C/D/E/F | 2-122 | [»] | |
5D6I | X-ray | 3.09 | A/B/C | 2-122 | [»] | |
5DWK | X-ray | 2.60 | A/B/C/D/E/F | 2-122 | [»] | |
BMRBi | P0ABN1 | |||||
SMRi | P0ABN1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261719, 281 interactors 852837, 2 interactors |
DIPi | DIP-60228N |
STRINGi | 511145.b4042 |
Chemistry databases
SwissLipidsi | SLP:000001806 |
Proteomic databases
jPOSTi | P0ABN1 |
PaxDbi | P0ABN1 |
PRIDEi | P0ABN1 |
Genome annotation databases
EnsemblBacteriai | AAC77012; AAC77012; b4042 BAE78044; BAE78044; BAE78044 |
GeneIDi | 48723107 948543 |
KEGGi | ecj:JW4002 eco:b4042 |
PATRICi | fig|1411691.4.peg.2666 |
Organism-specific databases
EchoBASEi | EB0220 |
Phylogenomic databases
eggNOGi | COG0818, Bacteria |
HOGENOMi | CLU_112343_3_1_6 |
InParanoidi | P0ABN1 |
PhylomeDBi | P0ABN1 |
Enzyme and pathway databases
BioCyci | EcoCyc:DIACYLGLYKIN-MONOMER MetaCyc:DIACYLGLYKIN-MONOMER |
BRENDAi | 2.7.1.107, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0ABN1 |
PROi | PR:P0ABN1 |
Family and domain databases
CDDi | cd14264, DAGK_IM, 1 hit |
Gene3Di | 1.10.3830.10, 1 hit |
InterProi | View protein in InterPro IPR000829, DAGK IPR033718, DAGK_prok IPR036945, DAGK_sf |
PANTHERi | PTHR34299, PTHR34299, 1 hit |
Pfami | View protein in Pfam PF01219, DAGK_prokar, 1 hit |
PROSITEi | View protein in PROSITE PS01069, DAGK_PROKAR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KDGL_ECOLI | |
Accessioni | P0ABN1Primary (citable) accession number: P0ABN1 Secondary accession number(s): P00556, Q2M6R2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 118 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families