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Protein

Cysteine synthase A

Gene

cysK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

In addition to its role in cysteine synthesis, stimulates the tRNase activity of CdiA-CT from E.coli strain 536 / UPEC; stimulation does not require O-acetylserine sulfhydrylase activity. CdiA is the toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria in a contact-dependent fashion (experiments done in strains BW25113 and X90, both K12 derivatives). This protein is not required for CDI of strain EC93, whose toxin may function by forming inner cell membrane pores.1 Publication

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.1 Publication
3-chloro-L-alanine + thioglycolate = S-carboxymethyl-L-cysteine + chloride.1 Publication

Cofactori

pyridoxal 5'-phosphate1 Publication

Kineticsi

  1. KM=40 mM for 3-chloro-L-alanine1 Publication
  2. KM=15.4 mM for thioglycolate1 Publication

    pH dependencei

    Optimum pH is 9-10.5.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius.1 Publication

    Pathwayi: L-cysteine biosynthesis

    This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Serine acetyltransferase (cysE)
    2. Cysteine synthase B (cysM), Cysteine synthase A (cysK)
    This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei8Allosteric inhibitorBy similarity1
    Binding sitei72Pyridoxal phosphateBy similarity1
    Binding sitei269Allosteric inhibitor; via amide nitrogenBy similarity1
    Binding sitei273Pyridoxal phosphateBy similarity1

    GO - Molecular functioni

    • cysteine synthase activity Source: EcoliWiki
    • L-cysteine desulfhydrase activity Source: EcoCyc
    • pyridoxal phosphate binding Source: EcoCyc
    • S-carboxymethylcysteine synthase activity Source: UniProtKB-EC
    • transferase activity Source: EcoliWiki

    GO - Biological processi

    • cellular amino acid biosynthetic process Source: EcoliWiki
    • cysteine biosynthetic process from serine Source: EcoCyc

    Keywordsi

    Molecular functionAllosteric enzyme, Lyase, Transferase
    Biological processAmino-acid biosynthesis, Cysteine biosynthesis
    LigandPyridoxal phosphate

    Enzyme and pathway databases

    BioCyciEcoCyc:ACSERLYA-MONOMER
    MetaCyc:ACSERLYA-MONOMER
    SABIO-RKiP0ABK5
    UniPathwayiUPA00136; UER00200

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine synthase A (EC:2.5.1.47)
    Short name:
    CSase A
    Alternative name(s):
    O-acetylserine (thiol)-lyase A
    Short name:
    OAS-TL A
    O-acetylserine sulfhydrylase A
    S-carboxymethylcysteine synthase (EC:4.5.1.51 Publication)
    Sulfate starvation-induced protein 5
    Short name:
    SSI5
    Gene namesi
    Name:cysK
    Synonyms:cysZ
    Ordered Locus Names:b2414, JW2407
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10192 cysK

    Subcellular locationi

    GO - Cellular componenti

    • cysteine synthase complex Source: EcoCyc
    • cytosol Source: EcoCyc

    Pathology & Biotechi

    Disruption phenotypei

    Significant decrease in tRNA nuclease activity of contact-dependent growth inhibitor CdiA-CT from strain 536 / UPEC (deletion in strain BW25113) (PubMed:22333533).1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi42K → A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved5 Publications
    ChainiPRO_00001670862 – 323Cysteine synthase AAdd BLAST322

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei42N6-(pyridoxal phosphate)lysineBy similarity1

    Proteomic databases

    EPDiP0ABK5
    PaxDbiP0ABK5
    PRIDEiP0ABK5

    2D gel databases

    SWISS-2DPAGEiP0ABK5

    Expressioni

    Inductioni

    Repressed by sulfate or cysteine.

    Interactioni

    Subunit structurei

    Homodimer (Ref. 6). Forms a cysteine synthase complex with 1 copy of CysE (By similarity). Interacts with CdiA-CT from strain 536 / UPEC, this is blocked upon preincubation with O-acetyl-L-serine. CysK forms a complex with CdiA-CT/CdiI (PubMed:22333533).By similarity2 Publications

    Protein-protein interaction databases

    BioGridi4259701, 23 interactors
    DIPiDIP-36170N
    IntActiP0ABK5, 14 interactors
    STRINGi316385.ECDH10B_2579

    Structurei

    3D structure databases

    ProteinModelPortaliP0ABK5
    SMRiP0ABK5
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni177 – 181Pyridoxal phosphate bindingBy similarity5

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105C6T Bacteria
    COG0031 LUCA
    HOGENOMiHOG000217394
    InParanoidiP0ABK5
    KOiK01738
    OMAiWDSGERY
    PhylomeDBiP0ABK5

    Family and domain databases

    InterProiView protein in InterPro
    IPR005856 Cys_synth
    IPR005859 CysK
    IPR001216 P-phosphate_BS
    IPR001926 PLP-dep
    IPR036052 Trypto_synt_PLP_dependent
    PfamiView protein in Pfam
    PF00291 PALP, 1 hit
    SUPFAMiSSF53686 SSF53686, 1 hit
    TIGRFAMsiTIGR01139 cysK, 1 hit
    TIGR01136 cysKM, 1 hit
    PROSITEiView protein in PROSITE
    PS00901 CYS_SYNTHASE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0ABK5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSKIFEDNSL TIGHTPLVRL NRIGNGRILA KVESRNPSFS VKCRIGANMI
    60 70 80 90 100
    WDAEKRGVLK PGVELVEPTS GNTGIALAYV AAARGYKLTL TMPETMSIER
    110 120 130 140 150
    RKLLKALGAN LVLTEGAKGM KGAIQKAEEI VASNPEKYLL LQQFSNPANP
    160 170 180 190 200
    EIHEKTTGPE IWEDTDGQVD VFIAGVGTGG TLTGVSRYIK GTKGKTDLIS
    210 220 230 240 250
    VAVEPTDSPV IAQALAGEEI KPGPHKIQGI GAGFIPANLD LKLVDKVIGI
    260 270 280 290 300
    TNEEAISTAR RLMEEEGILA GISSGAAVAA ALKLQEDESF TNKNIVVILP
    310 320
    SSGERYLSTA LFADLFTEKE LQQ
    Length:323
    Mass (Da):34,490
    Last modified:January 23, 2007 - v2
    Checksum:i20ADDE911845AE72
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti182L → W in AAA23654 (PubMed:3290198).Curated1
    Sequence conflicti186 – 187SR → TP in AAA23654 (PubMed:3290198).Curated2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X12615 Genomic DNA Translation: CAA31137.1
    M21451 Genomic DNA Translation: AAA23654.1
    U00096 Genomic DNA Translation: AAC75467.1
    AP009048 Genomic DNA Translation: BAA16288.1
    M21994 Genomic DNA Translation: AAA24383.1
    PIRiE65015 SYECAC
    RefSeqiNP_416909.1, NC_000913.3
    WP_000034402.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC75467; AAC75467; b2414
    BAA16288; BAA16288; BAA16288
    GeneIDi946877
    KEGGiecj:JW2407
    eco:b2414
    PATRICifig|1411691.4.peg.4317

    Similar proteinsi

    Entry informationi

    Entry nameiCYSK_ECOLI
    AccessioniPrimary (citable) accession number: P0ABK5
    Secondary accession number(s): P11096, P21546
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: January 23, 2007
    Last modified: March 28, 2018
    This is version 103 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

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