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Protein

Cytochrome bo(3) ubiquinol oxidase subunit 2

Gene

cyoA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytochrome bo3 ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron.3 Publications

Activity regulationi

Competitively inhibited by piericidin A, non-competitively inhibited by 2-n-heptyl-4-hydroxyquinoline N-oxide, NaN3 and KCN; 50% inhibition occurs at 2 µM, 2 µM, 15 mM and 10 µM, respectively. Inhibited by Zn2+ and Cd2+.1 Publication

GO - Molecular functioni

GO - Biological processi

  • aerobic electron transport chain Source: EcoCyc
  • aerobic respiration Source: EcoCyc
  • ATP synthesis coupled electron transport Source: GO_Central
  • electron transport coupled proton transport Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CYOA-MONOMER
MetaCyc:CYOA-MONOMER
BRENDAi1.10.3.10 2026

Protein family/group databases

TCDBi3.D.4.5.1 the proton-translocating cytochrome oxidase (cox) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome bo(3) ubiquinol oxidase subunit 2
Alternative name(s):
Cytochrome b562-o complex subunit II
Cytochrome o ubiquinol oxidase subunit 2
Short name:
Cytochrome o subunit 2
Oxidase bo(3) subunit 2
Ubiquinol oxidase chain B
Ubiquinol oxidase polypeptide II
Ubiquinol oxidase subunit 2
Gene namesi
Name:cyoA
Ordered Locus Names:b0432, JW0422
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10178 cyoA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 50PeriplasmicCuratedAdd BLAST26
Transmembranei51 – 68HelicalCuratedAdd BLAST18
Topological domaini69 – 92CytoplasmicCuratedAdd BLAST24
Transmembranei93 – 111HelicalCuratedAdd BLAST19
Topological domaini112 – 315PeriplasmicCuratedAdd BLAST204

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Increased reduction of the ubiquinone pool (in aerobically grown minimal medium with glucose).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24PROSITE-ProRule annotationAdd BLAST24
ChainiPRO_000000607125 – 315Cytochrome bo(3) ubiquinol oxidase subunit 2Add BLAST291

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi25N-palmitoyl cysteinePROSITE-ProRule annotation1
Lipidationi25S-diacylglycerol cysteinePROSITE-ProRule annotation1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

EPDiP0ABJ1
PaxDbiP0ABJ1
PRIDEiP0ABJ1

Interactioni

Subunit structurei

Heterooctamer of two A chains, two B chains, two C chains and two D chains.3 Publications

Protein-protein interaction databases

BioGridi4259512, 300 interactors
ComplexPortaliCPX-2102 Cytochrome o ubiquinol oxidase complex
DIPiDIP-47942N
IntActiP0ABJ1, 23 interactors
STRINGi316385.ECDH10B_0388

Structurei

Secondary structure

1315
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00089
ProteinModelPortaliP0ABJ1
SMRiP0ABJ1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ABJ1

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105E2S Bacteria
COG1622 LUCA
HOGENOMiHOG000084157
InParanoidiP0ABJ1
KOiK02297
PhylomeDBiP0ABJ1

Family and domain databases

CDDicd04212 CuRO_UO_II, 1 hit
Gene3Di1.10.287.90, 1 hit
2.60.40.420, 1 hit
InterProiView protein in InterPro
IPR002429 CcO_II-like_C
IPR010514 COX_ARM
IPR008972 Cupredoxin
IPR034227 CuRO_UO_II
IPR011759 Cyt_c_oxidase_su2_TM_dom
IPR036257 Cyt_c_oxidase_su2_TM_sf
IPR006333 Cyt_o_ubiquinol_oxidase_su2
PfamiView protein in Pfam
PF00116 COX2, 1 hit
PF06481 COX_ARM, 1 hit
PIRSFiPIRSF000292 Ubi_od_II, 1 hit
SUPFAMiSSF49503 SSF49503, 1 hit
SSF81464 SSF81464, 1 hit
TIGRFAMsiTIGR01433 CyoA, 1 hit
PROSITEiView protein in PROSITE
PS50857 COX2_CUA, 1 hit
PS50999 COX2_TM, 1 hit
PS51257 PROKAR_LIPOPROTEIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABJ1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRLRKYNKSL GWLSLFAGTV LLSGCNSALL DPKGQIGLEQ RSLILTAFGL
60 70 80 90 100
MLIVVIPAIL MAVGFAWKYR ASNKDAKYSP NWSHSNKVEA VVWTVPILII
110 120 130 140 150
IFLAVLTWKT THALEPSKPL AHDEKPITIE VVSMDWKWFF IYPEQGIATV
160 170 180 190 200
NEIAFPANTP VYFKVTSNSV MNSFFIPRLG SQIYAMAGMQ TRLHLIANEP
210 220 230 240 250
GTYDGISASY SGPGFSGMKF KAIATPDRAA FDQWVAKAKQ SPNTMSDMAA
260 270 280 290 300
FEKLAAPSEY NQVEYFSNVK PDLFADVINK FMAHGKSMDM TQPEGEHSAH
310
EGMEGMDMSH AESAH
Length:315
Mass (Da):34,911
Last modified:October 25, 2005 - v1
Checksum:i62EC951183B65724
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05492 Genomic DNA Translation: AAA23631.1
U82664 Genomic DNA Translation: AAB40188.1
U00096 Genomic DNA Translation: AAC73535.1
AP009048 Genomic DNA Translation: BAE76212.1
M55258 Genomic DNA Translation: AAA23630.1
S67816 Genomic DNA Translation: AAB28885.1
PIRiA42226
RefSeqiNP_414966.1, NC_000913.3
WP_001239436.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73535; AAC73535; b0432
BAE76212; BAE76212; BAE76212
GeneIDi945080
KEGGiecj:JW0422
eco:b0432
PATRICifig|1411691.4.peg.1845

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05492 Genomic DNA Translation: AAA23631.1
U82664 Genomic DNA Translation: AAB40188.1
U00096 Genomic DNA Translation: AAC73535.1
AP009048 Genomic DNA Translation: BAE76212.1
M55258 Genomic DNA Translation: AAA23630.1
S67816 Genomic DNA Translation: AAB28885.1
PIRiA42226
RefSeqiNP_414966.1, NC_000913.3
WP_001239436.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CYWX-ray2.50A111-315[»]
1CYXX-ray2.30A111-315[»]
1FFTX-ray3.50B/G1-315[»]
DisProtiDP00089
ProteinModelPortaliP0ABJ1
SMRiP0ABJ1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259512, 300 interactors
ComplexPortaliCPX-2102 Cytochrome o ubiquinol oxidase complex
DIPiDIP-47942N
IntActiP0ABJ1, 23 interactors
STRINGi316385.ECDH10B_0388

Protein family/group databases

TCDBi3.D.4.5.1 the proton-translocating cytochrome oxidase (cox) superfamily

Proteomic databases

EPDiP0ABJ1
PaxDbiP0ABJ1
PRIDEiP0ABJ1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73535; AAC73535; b0432
BAE76212; BAE76212; BAE76212
GeneIDi945080
KEGGiecj:JW0422
eco:b0432
PATRICifig|1411691.4.peg.1845

Organism-specific databases

EchoBASEiEB0175
EcoGeneiEG10178 cyoA

Phylogenomic databases

eggNOGiENOG4105E2S Bacteria
COG1622 LUCA
HOGENOMiHOG000084157
InParanoidiP0ABJ1
KOiK02297
PhylomeDBiP0ABJ1

Enzyme and pathway databases

BioCyciEcoCyc:CYOA-MONOMER
MetaCyc:CYOA-MONOMER
BRENDAi1.10.3.10 2026

Miscellaneous databases

EvolutionaryTraceiP0ABJ1
PROiPR:P0ABJ1

Family and domain databases

CDDicd04212 CuRO_UO_II, 1 hit
Gene3Di1.10.287.90, 1 hit
2.60.40.420, 1 hit
InterProiView protein in InterPro
IPR002429 CcO_II-like_C
IPR010514 COX_ARM
IPR008972 Cupredoxin
IPR034227 CuRO_UO_II
IPR011759 Cyt_c_oxidase_su2_TM_dom
IPR036257 Cyt_c_oxidase_su2_TM_sf
IPR006333 Cyt_o_ubiquinol_oxidase_su2
PfamiView protein in Pfam
PF00116 COX2, 1 hit
PF06481 COX_ARM, 1 hit
PIRSFiPIRSF000292 Ubi_od_II, 1 hit
SUPFAMiSSF49503 SSF49503, 1 hit
SSF81464 SSF81464, 1 hit
TIGRFAMsiTIGR01433 CyoA, 1 hit
PROSITEiView protein in PROSITE
PS50857 COX2_CUA, 1 hit
PS50999 COX2_TM, 1 hit
PS51257 PROKAR_LIPOPROTEIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCYOA_ECOLI
AccessioniPrimary (citable) accession number: P0ABJ1
Secondary accession number(s): P18400, Q2MBZ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: November 7, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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