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UniProtKB - P0ABJ1 (CYOA_ECOLI)
Protein
Cytochrome bo(3) ubiquinol oxidase subunit 2
Gene
cyoA
Organism
Escherichia coli (strain K12)
Status
Functioni
Cytochrome bo3 ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron.
3 PublicationsActivity regulationi
Competitively inhibited by piericidin A, non-competitively inhibited by 2-n-heptyl-4-hydroxyquinoline N-oxide, NaN3 and KCN; 50% inhibition occurs at 2 µM, 2 µM, 15 mM and 10 µM, respectively. Inhibited by Zn2+ and Cd2+.1 Publication
GO - Molecular functioni
- copper ion binding Source: InterPro
- cytochrome bo3 ubiquinol oxidase activity Source: EcoCyc
- cytochrome-c oxidase activity Source: InterPro
- electron transfer activity Source: EcoCyc
- oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor Source: InterPro
- oxidoreduction-driven active transmembrane transporter activity Source: EcoCyc
- proton transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- aerobic electron transport chain Source: ComplexPortal
- aerobic respiration Source: EcoCyc
- ATP synthesis coupled electron transport Source: GO_Central
- electron transport coupled proton transport Source: ComplexPortal
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Respiratory chain, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:CYOA-MONOMER |
BRENDAi | 7.1.1.3, 2026 |
Protein family/group databases
TCDBi | 3.D.4.5.1, the proton-translocating cytochrome oxidase (cox) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome bo(3) ubiquinol oxidase subunit 2Alternative name(s): Cytochrome b562-o complex subunit II Cytochrome o ubiquinol oxidase subunit 2 Short name: Cytochrome o subunit 2 Oxidase bo(3) subunit 2 Ubiquinol oxidase chain B Ubiquinol oxidase polypeptide II Ubiquinol oxidase subunit 2 |
Gene namesi | Name:cyoA Ordered Locus Names:b0432, JW0422 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 2 Publications; Multi-pass membrane protein 2 Publications
Plasma Membrane
- integral component of external side of plasma membrane Source: EcoCyc
- integral component of plasma membrane Source: EcoliWiki
- plasma membrane Source: ComplexPortal
Other locations
- cytochrome o ubiquinol oxidase complex Source: EcoCyc
- respirasome Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 25 – 50 | PeriplasmicCuratedAdd BLAST | 26 | |
Transmembranei | 51 – 68 | HelicalCuratedAdd BLAST | 18 | |
Topological domaini | 69 – 92 | CytoplasmicCuratedAdd BLAST | 24 | |
Transmembranei | 93 – 111 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 112 – 315 | PeriplasmicCuratedAdd BLAST | 204 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Increased reduction of the ubiquinone pool (in aerobically grown minimal medium with glucose).2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | PROSITE-ProRule annotationAdd BLAST | 24 | |
ChainiPRO_0000006071 | 25 – 315 | Cytochrome bo(3) ubiquinol oxidase subunit 2Add BLAST | 291 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 25 | N-palmitoyl cysteinePROSITE-ProRule annotation | 1 | |
Lipidationi | 25 | S-diacylglycerol cysteinePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
jPOSTi | P0ABJ1 |
PaxDbi | P0ABJ1 |
PRIDEi | P0ABJ1 |
Interactioni
Subunit structurei
Heterooctamer of two A chains, two B chains, two C chains and two D chains.
3 PublicationsProtein-protein interaction databases
BioGRIDi | 4259512, 300 interactors |
ComplexPortali | CPX-2102, Cytochrome bo3 ubiquinol oxidase complex |
DIPi | DIP-47942N |
IntActi | P0ABJ1, 23 interactors |
STRINGi | 511145.b0432 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0ABJ1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ABJ1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 288 – 315 | DisorderedSequence analysisAdd BLAST | 28 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 290 – 315 | Basic and acidic residuesSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Belongs to the cytochrome c oxidase subunit 2 family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1622, Bacteria |
HOGENOMi | CLU_036876_6_1_6 |
InParanoidi | P0ABJ1 |
PhylomeDBi | P0ABJ1 |
Family and domain databases
CDDi | cd04212, CuRO_UO_II, 1 hit |
Gene3Di | 1.10.287.90, 1 hit 2.60.40.420, 1 hit |
InterProi | View protein in InterPro IPR045187, CcO_II IPR002429, CcO_II-like_C IPR010514, COX_ARM IPR008972, Cupredoxin IPR034227, CuRO_UO_II IPR011759, Cyt_c_oxidase_su2_TM_dom IPR036257, Cyt_c_oxidase_su2_TM_sf IPR006333, Cyt_o_ubiquinol_oxidase_su2 |
PANTHERi | PTHR22888, PTHR22888, 1 hit |
Pfami | View protein in Pfam PF00116, COX2, 1 hit PF06481, COX_ARM, 1 hit |
PIRSFi | PIRSF000292, Ubi_od_II, 1 hit |
SUPFAMi | SSF49503, SSF49503, 1 hit SSF81464, SSF81464, 1 hit |
TIGRFAMsi | TIGR01433, CyoA, 1 hit |
PROSITEi | View protein in PROSITE PS50857, COX2_CUA, 1 hit PS50999, COX2_TM, 1 hit PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0ABJ1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLRKYNKSL GWLSLFAGTV LLSGCNSALL DPKGQIGLEQ RSLILTAFGL
60 70 80 90 100
MLIVVIPAIL MAVGFAWKYR ASNKDAKYSP NWSHSNKVEA VVWTVPILII
110 120 130 140 150
IFLAVLTWKT THALEPSKPL AHDEKPITIE VVSMDWKWFF IYPEQGIATV
160 170 180 190 200
NEIAFPANTP VYFKVTSNSV MNSFFIPRLG SQIYAMAGMQ TRLHLIANEP
210 220 230 240 250
GTYDGISASY SGPGFSGMKF KAIATPDRAA FDQWVAKAKQ SPNTMSDMAA
260 270 280 290 300
FEKLAAPSEY NQVEYFSNVK PDLFADVINK FMAHGKSMDM TQPEGEHSAH
310
EGMEGMDMSH AESAH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05492 Genomic DNA Translation: AAA23631.1 U82664 Genomic DNA Translation: AAB40188.1 U00096 Genomic DNA Translation: AAC73535.1 AP009048 Genomic DNA Translation: BAE76212.1 M55258 Genomic DNA Translation: AAA23630.1 S67816 Genomic DNA Translation: AAB28885.1 |
PIRi | A42226 |
RefSeqi | NP_414966.1, NC_000913.3 WP_001239436.1, NZ_SSZK01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC73535; AAC73535; b0432 BAE76212; BAE76212; BAE76212 |
GeneIDi | 67416493 945080 |
KEGGi | ecj:JW0422 eco:b0432 |
PATRICi | fig|1411691.4.peg.1845 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05492 Genomic DNA Translation: AAA23631.1 U82664 Genomic DNA Translation: AAB40188.1 U00096 Genomic DNA Translation: AAC73535.1 AP009048 Genomic DNA Translation: BAE76212.1 M55258 Genomic DNA Translation: AAA23630.1 S67816 Genomic DNA Translation: AAB28885.1 |
PIRi | A42226 |
RefSeqi | NP_414966.1, NC_000913.3 WP_001239436.1, NZ_SSZK01000009.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CYW | X-ray | 2.50 | A | 111-315 | [»] | |
1CYX | X-ray | 2.30 | A | 111-315 | [»] | |
1FFT | X-ray | 3.50 | B/G | 1-315 | [»] | |
7CUB | electron microscopy | 2.55 | B | 25-315 | [»] | |
7CUQ | electron microscopy | 2.64 | B | 25-315 | [»] | |
7CUW | electron microscopy | 2.63 | B | 25-315 | [»] | |
SMRi | P0ABJ1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259512, 300 interactors |
ComplexPortali | CPX-2102, Cytochrome bo3 ubiquinol oxidase complex |
DIPi | DIP-47942N |
IntActi | P0ABJ1, 23 interactors |
STRINGi | 511145.b0432 |
Protein family/group databases
TCDBi | 3.D.4.5.1, the proton-translocating cytochrome oxidase (cox) superfamily |
Proteomic databases
jPOSTi | P0ABJ1 |
PaxDbi | P0ABJ1 |
PRIDEi | P0ABJ1 |
Genome annotation databases
EnsemblBacteriai | AAC73535; AAC73535; b0432 BAE76212; BAE76212; BAE76212 |
GeneIDi | 67416493 945080 |
KEGGi | ecj:JW0422 eco:b0432 |
PATRICi | fig|1411691.4.peg.1845 |
Organism-specific databases
EchoBASEi | EB0175 |
Phylogenomic databases
eggNOGi | COG1622, Bacteria |
HOGENOMi | CLU_036876_6_1_6 |
InParanoidi | P0ABJ1 |
PhylomeDBi | P0ABJ1 |
Enzyme and pathway databases
BioCyci | EcoCyc:CYOA-MONOMER |
BRENDAi | 7.1.1.3, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0ABJ1 |
PROi | PR:P0ABJ1 |
Family and domain databases
CDDi | cd04212, CuRO_UO_II, 1 hit |
Gene3Di | 1.10.287.90, 1 hit 2.60.40.420, 1 hit |
InterProi | View protein in InterPro IPR045187, CcO_II IPR002429, CcO_II-like_C IPR010514, COX_ARM IPR008972, Cupredoxin IPR034227, CuRO_UO_II IPR011759, Cyt_c_oxidase_su2_TM_dom IPR036257, Cyt_c_oxidase_su2_TM_sf IPR006333, Cyt_o_ubiquinol_oxidase_su2 |
PANTHERi | PTHR22888, PTHR22888, 1 hit |
Pfami | View protein in Pfam PF00116, COX2, 1 hit PF06481, COX_ARM, 1 hit |
PIRSFi | PIRSF000292, Ubi_od_II, 1 hit |
SUPFAMi | SSF49503, SSF49503, 1 hit SSF81464, SSF81464, 1 hit |
TIGRFAMsi | TIGR01433, CyoA, 1 hit |
PROSITEi | View protein in PROSITE PS50857, COX2_CUA, 1 hit PS50999, COX2_TM, 1 hit PS51257, PROKAR_LIPOPROTEIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CYOA_ECOLI | |
Accessioni | P0ABJ1Primary (citable) accession number: P0ABJ1 Secondary accession number(s): P18400, Q2MBZ4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | October 25, 2005 | |
Last modified: | February 23, 2022 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families