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UniProtKB - P0ABH9 (CLPA_ECOLI)
Protein
ATP-dependent Clp protease ATP-binding subunit ClpA
Gene
clpA
Organism
Escherichia coli (strain K12)
Status
Functioni
ATP-dependent specificity component of the ClpAP protease. It directs the protease to specific substrates. It has unfoldase activity. The primary function of the ClpA-ClpP complex appears to be the degradation of unfolded or abnormal proteins.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 214 – 221 | ATPSequence analysis | 8 | |
Nucleotide bindingi | 495 – 502 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: EcoCyc
- ATP-dependent peptidase activity Source: CACAO
- ATP hydrolysis activity Source: EcoCyc
GO - Biological processi
- cellular response to heat Source: GO_Central
- protein unfolding Source: EcoCyc
- response to oxidative stress Source: EcoCyc
Keywordsi
Molecular function | Chaperone |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10156-MONOMER |
SABIO-RKi | P0ABH9 |
Protein family/group databases
MEROPSi | X20.001 |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent Clp protease ATP-binding subunit ClpA |
Gene namesi | Name:clpA Synonyms:lopD Ordered Locus Names:b0882, JW0866 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: EcoCyc
Other locations
- cytoplasm Source: GO_Central
- endopeptidase Clp complex Source: ComplexPortal
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191079 | 1 – 758 | ATP-dependent Clp protease ATP-binding subunit ClpAAdd BLAST | 758 |
Proteomic databases
jPOSTi | P0ABH9 |
PaxDbi | P0ABH9 |
PRIDEi | P0ABH9 |
Interactioni
Subunit structurei
Component of the ClpAP complex composed of six ClpA subunits assembled into a hexameric ring in the presence of ATP, and fourteen ClpP subunits arranged in two heptameric rings. Binds to ClpS.
2 PublicationsBinary interactionsi
P0ABH9
With | #Exp. | IntAct |
---|---|---|
barA [P0AEC5] | 3 | EBI-546140,EBI-546740 |
clpP [P0A6G7] | 11 | EBI-546140,EBI-370625 |
clpS [P0A8Q6] | 13 | EBI-546140,EBI-561456 |
fixX [P68646] | 2 | EBI-546140,EBI-1113234 |
Protein-protein interaction databases
BioGRIDi | 4261320, 452 interactors 850131, 5 interactors |
ComplexPortali | CPX-3175, Endopeptidase ClpAP complex |
DIPi | DIP-35409N |
IntActi | P0ABH9, 77 interactors |
MINTi | P0ABH9 |
STRINGi | 511145.b0882 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0ABH9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0ABH9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 145 | Clp RPROSITE-ProRule annotationAdd BLAST | 145 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 65 | Repeat 1PROSITE-ProRule annotationAdd BLAST | 64 | |
Regioni | 80 – 145 | Repeat 2PROSITE-ProRule annotationAdd BLAST | 66 | |
Regioni | 140 – 167 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 169 – 417 | IAdd BLAST | 249 | |
Regioni | 421 – 609 | IIAdd BLAST | 189 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 142 – 167 | Polar residuesSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Belongs to the ClpA/ClpB family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG0542, Bacteria |
HOGENOMi | CLU_005070_4_2_6 |
InParanoidi | P0ABH9 |
PhylomeDBi | P0ABH9 |
Family and domain databases
Gene3Di | 1.10.1780.10, 1 hit 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003959, ATPase_AAA_core IPR019489, Clp_ATPase_C IPR036628, Clp_N_dom_sf IPR004176, Clp_R_dom IPR013461, ClpA IPR001270, ClpA/B IPR018368, ClpA/B_CS1 IPR028299, ClpA/B_CS2 IPR041546, ClpA/ClpB_AAA_lid IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF07724, AAA_2, 1 hit PF17871, AAA_lid_9, 1 hit PF02861, Clp_N, 1 hit PF10431, ClpB_D2-small, 1 hit |
PRINTSi | PR00300, CLPPROTEASEA |
SMARTi | View protein in SMART SM00382, AAA, 2 hits SM01086, ClpB_D2-small, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF81923, SSF81923, 1 hit |
TIGRFAMsi | TIGR02639, ClpA, 1 hit |
PROSITEi | View protein in PROSITE PS51903, CLP_R, 1 hit PS00870, CLPAB_1, 1 hit PS00871, CLPAB_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0ABH9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLNQELELSL NMAFARAREH RHEFMTVEHL LLALLSNPSA REALEACSVD
60 70 80 90 100
LVALRQELEA FIEQTTPVLP ASEEERDTQP TLSFQRVLQR AVFHVQSSGR
110 120 130 140 150
NEVTGANVLV AIFSEQESQA AYLLRKHEVS RLDVVNFISH GTRKDEPTQS
160 170 180 190 200
SDPGSQPNSE EQAGGEERME NFTTNLNQLA RVGGIDPLIG REKELERAIQ
210 220 230 240 250
VLCRRRKNNP LLVGESGVGK TAIAEGLAWR IVQGDVPEVM ADCTIYSLDI
260 270 280 290 300
GSLLAGTKYR GDFEKRFKAL LKQLEQDTNS ILFIDEIHTI IGAGAASGGQ
310 320 330 340 350
VDAANLIKPL LSSGKIRVIG STTYQEFSNI FEKDRALARR FQKIDITEPS
360 370 380 390 400
IEETVQIING LKPKYEAHHD VRYTAKAVRA AVELAVKYIN DRHLPDKAID
410 420 430 440 450
VIDEAGARAR LMPVSKRKKT VNVADIESVV ARIARIPEKS VSQSDRDTLK
460 470 480 490 500
NLGDRLKMLV FGQDKAIEAL TEAIKMARAG LGHEHKPVGS FLFAGPTGVG
510 520 530 540 550
KTEVTVQLSK ALGIELLRFD MSEYMERHTV SRLIGAPPGY VGFDQGGLLT
560 570 580 590 600
DAVIKHPHAV LLLDEIEKAH PDVFNILLQV MDNGTLTDNN GRKADFRNVV
610 620 630 640 650
LVMTTNAGVR ETERKSIGLI HQDNSTDAME EIKKIFTPEF RNRLDNIIWF
660 670 680 690 700
DHLSTDVIHQ VVDKFIVELQ VQLDQKGVSL EVSQEARNWL AEKGYDRAMG
710 720 730 740 750
ARPMARVIQD NLKKPLANEL LFGSLVDGGQ VTVALDKEKN ELTYGFQSAQ
KHKAEAAH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 367 | A → G in AAA23583 (PubMed:2186030).Curated | 1 | |
Sequence conflicti | 411 | L → V in AAA23583 (PubMed:2186030).Curated | 1 | |
Sequence conflicti | 533 | L → V in AAA23583 (PubMed:2186030).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31045 Genomic DNA Translation: AAA23583.1 U00096 Genomic DNA Translation: AAC73969.1 AP009048 Genomic DNA Translation: BAA35601.1 M23220 Genomic DNA Translation: AAA23582.1 |
PIRi | B64827, SUECCA |
RefSeqi | NP_415403.1, NC_000913.3 WP_000934041.1, NZ_STEB01000006.1 |
Genome annotation databases
EnsemblBacteriai | AAC73969; AAC73969; b0882 BAA35601; BAA35601; BAA35601 |
GeneIDi | 66670844 945764 |
KEGGi | ecj:JW0866 eco:b0882 |
PATRICi | fig|1411691.4.peg.1395 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31045 Genomic DNA Translation: AAA23583.1 U00096 Genomic DNA Translation: AAC73969.1 AP009048 Genomic DNA Translation: BAA35601.1 M23220 Genomic DNA Translation: AAA23582.1 |
PIRi | B64827, SUECCA |
RefSeqi | NP_415403.1, NC_000913.3 WP_000934041.1, NZ_STEB01000006.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1K6K | X-ray | 1.80 | A | 1-143 | [»] | |
1KSF | X-ray | 2.60 | X | 1-758 | [»] | |
1LZW | X-ray | 2.50 | B | 1-146 | [»] | |
1MBU | X-ray | 2.30 | A/B | 1-142 | [»] | |
1MBV | X-ray | 3.30 | A | 1-142 | [»] | |
1MBX | X-ray | 2.25 | A/B | 1-142 | [»] | |
1MG9 | X-ray | 2.30 | B | 1-146 | [»] | |
1R6B | X-ray | 2.25 | X | 1-758 | [»] | |
1R6C | X-ray | 2.15 | X | 1-143 | [»] | |
1R6O | X-ray | 2.25 | A/B | 1-143 | [»] | |
1R6Q | X-ray | 2.35 | A/B | 1-143 | [»] | |
5OFO | electron microscopy | 4.60 | A/B/C/D/E/F | 609-635 | [»] | |
5OG1 | electron microscopy | 4.50 | A/B/C/D/E/F | 609-635 | [»] | |
6UQE | electron microscopy | 3.00 | A/B/C/D/E/F | 169-746 | [»] | |
6UQO | electron microscopy | 3.10 | A/B/C/D/E/F | 169-746 | [»] | |
6W1Z | electron microscopy | 2.70 | A/B/C/D/E/F | 1-758 | [»] | |
6W20 | electron microscopy | 3.00 | A/B/C/D/E/F | 1-758 | [»] | |
6W21 | electron microscopy | 3.30 | A/B/C/D/E/F | 1-758 | [»] | |
6W22 | electron microscopy | 3.00 | A/B/C/D/E/F | 1-758 | [»] | |
6W23 | electron microscopy | 3.10 | A/B/C/D/E/F | 1-758 | [»] | |
6W24 | electron microscopy | 3.40 | A/B/C/D/E/F | 1-758 | [»] | |
SMRi | P0ABH9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261320, 452 interactors 850131, 5 interactors |
ComplexPortali | CPX-3175, Endopeptidase ClpAP complex |
DIPi | DIP-35409N |
IntActi | P0ABH9, 77 interactors |
MINTi | P0ABH9 |
STRINGi | 511145.b0882 |
Protein family/group databases
MEROPSi | X20.001 |
Proteomic databases
jPOSTi | P0ABH9 |
PaxDbi | P0ABH9 |
PRIDEi | P0ABH9 |
Genome annotation databases
EnsemblBacteriai | AAC73969; AAC73969; b0882 BAA35601; BAA35601; BAA35601 |
GeneIDi | 66670844 945764 |
KEGGi | ecj:JW0866 eco:b0882 |
PATRICi | fig|1411691.4.peg.1395 |
Organism-specific databases
EchoBASEi | EB0154 |
Phylogenomic databases
eggNOGi | COG0542, Bacteria |
HOGENOMi | CLU_005070_4_2_6 |
InParanoidi | P0ABH9 |
PhylomeDBi | P0ABH9 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10156-MONOMER |
SABIO-RKi | P0ABH9 |
Miscellaneous databases
EvolutionaryTracei | P0ABH9 |
PROi | PR:P0ABH9 |
Family and domain databases
Gene3Di | 1.10.1780.10, 1 hit 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003959, ATPase_AAA_core IPR019489, Clp_ATPase_C IPR036628, Clp_N_dom_sf IPR004176, Clp_R_dom IPR013461, ClpA IPR001270, ClpA/B IPR018368, ClpA/B_CS1 IPR028299, ClpA/B_CS2 IPR041546, ClpA/ClpB_AAA_lid IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF07724, AAA_2, 1 hit PF17871, AAA_lid_9, 1 hit PF02861, Clp_N, 1 hit PF10431, ClpB_D2-small, 1 hit |
PRINTSi | PR00300, CLPPROTEASEA |
SMARTi | View protein in SMART SM00382, AAA, 2 hits SM01086, ClpB_D2-small, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF81923, SSF81923, 1 hit |
TIGRFAMsi | TIGR02639, ClpA, 1 hit |
PROSITEi | View protein in PROSITE PS51903, CLP_R, 1 hit PS00870, CLPAB_1, 1 hit PS00871, CLPAB_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CLPA_ECOLI | |
Accessioni | P0ABH9Primary (citable) accession number: P0ABH9 Secondary accession number(s): P15716, P77686 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | October 25, 2005 | |
Last modified: | February 23, 2022 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families